KR100447441B1 - Skin whitening agent containing bird's nest extract as tyrosinase inhibiting agent as main component and albutin or kojic acid as auxiliary component - Google Patents

Abstract

PURPOSE: A skin whitening agent containing the bird's nest extract as a tyrosinase inhibiting agent as a main component and albutin or kojic acid as an auxiliary component is provided. The agent exhibits superior whitening effect, solves problems, such as, temporal changes during storage and causes no skin irritation. CONSTITUTION: The skin whitening agent contains 0.01 to 10% by weight of a mixture of the bird's nest extract which helps inhibit tyrosinase activity and albutin or kojic acid, based on the total weight of the agent. For an example, 2.0% by weight of the bird's nest extract is mixed with 1.0% by weight of albutin, 6.0% by weight of fluid paraffin, 4.0% by weight of squalane, 0.5% by weight of sorbitan sesquiolate, 1.5% by weight of Polysorbate 60, 10.0% by weight of bees wax, 5.0% by weight of capric/caprylic triglyceride, 6.0% by weight of concentrated glycerine, 3.0% by weight of propylene glycol, 0.2% by weight of triethanolamine and a trace amount of antiseptics, pigments, fragrance and the balance of purified water to give nourishing cream.

Description

Blending Whitening Composition

The present invention relates to a mixed whitening composition, and more particularly, to a mixed whitening composition containing sea bird's nest extract extracted from sea bird's nest as a main component, and containing arbutin or kojic acid as auxiliary components.

Human skin color depends mainly on the number, size, type and distribution of melanocytes containing melanin contained in skin cells. Melanosomes are produced by melanocytes, and the produced melanin plays an important role in preventing skin damage and skin cancer caused by light rays by protecting the skin from excessive ultraviolet rays. However, pigmentation of the skin due to overproduction of melanin caused by daylight, hormonal changes, inflammation, and drugs causes various problems in terms of beauty. Hyperpigmentation of the skin, such as blemishes and freckles, is due to an increase in the amount of melanin, which is due to the accelerated melanogenesis process or an increase in the number of melanocytes.

The most important role in melanin formation is known as the tyrosinase enzyme, which acts on an amino acid called tyrosine in the tissue to produce dopaquinones and steps from this dopaquinone. It produces melanin through the automatic oxidation process.

Therefore, the method of using hydroquinone, tretinoin, kojic acid, arbutin, ascorbic acid, glutathione, and the like as a whitening ingredient in the cosmetic field in the prior art, by inhibiting the activity of tyrosinase enzyme, inhibiting melanin production, Some have been used. However, when manufacturing cosmetics using the above-mentioned ingredients, there is a disadvantage in that the coloration during the manufacturing process or the storage of the product, in order to reduce the coloration to use a large amount of additives or to add a small amount of the active ingredient, In this case, there may be a side effect caused by such additive components, so there is a problem in safety for the skin, and in the case of using an active ingredient in a small amount, there is a disadvantage in that sufficient whitening effects cannot be obtained as desired.

Therefore, while using a smaller amount than the existing whitening agent has excellent whitening effect, there is a need for the development of a whitening agent without side effects such as irritation to the skin.

The present inventors searched for substances that are effective for preventing hyperpigmentation among various natural products of nature in order to solve the problems of the conventional whitening materials and to find more excellent whitening materials, and among the whitening materials that can be used in cosmetics to date It has been found that extracts can be used as tyrosinase inhibitors.

In addition, the present inventors can compensate for the shortcomings of the existing whitening components due to the whitening synergy effect between the two components when using a combination of the existing whitening component and the bird's nest extract, and obtain the desired whitening effect even with a small amount of the formulation It was confirmed that the present invention was completed.

Accordingly, an object of the present invention is to provide a cosmetic whitening composition for cosmetics having a superior whitening effect and no side effects such as skin irritation while using a smaller amount than the existing whitening agent.

Figure 1A is a graph showing the inhibition rate of tyrosinase activity according to the change in the concentration of algae extract and arbutin.

Figure 1B is a graph showing the inhibition rate of tyrosinase activity according to the concentration of sea bird's nest extract or arbutin.

Figure 2A is a graph showing the inhibitory rate of tyrosinase activity according to the change in the concentration of the bird's nest extract and koji acid.

Figure 2B is a graph showing the inhibition rate of tyrosinase activity according to the concentration change of sea bird's nest extract or kojic acid.

The present invention provides a mixed whitening composition, which contains a bird's nest extract extracted from a bird's nest as a main component, and contains arbutin or kojic acid as an auxiliary component.

The bird's nest extract used in the present invention is extracted from the bird's nest nesting the sea material in the sea, the bird's nest is already marketed as food for food in Thailand, Phuket, Indonesia and Singapore, etc. In the ancient Chinese imperial family, queens and aristocrats have been known to have been used as an adjuvant to make the skin of the face and body beautiful and white.

The whitening composition according to the present invention can be applied to softening cream (skin), nutrient cream (lotion), nourishing cream, massage cream, essence or pack.

The present invention will be described in more detail as follows.

There are two kinds of sea bird's nest extract, liquid type and gel type according to the extraction method from sea bird's nest.

In the present invention, a commercial bird's nest extract (from CAMPO RESEARCH) was used, and the extract was placed in a 1: 1 mixture of ethanol and water, cooled and steamed and then gelled (Gel). ), Which is then mixed with ethanol in a ratio of 1: 2 to form a liquid formulation.

As components of the bird's nest extract used in the present invention, polysaccharide is 90%, L-ascorbic acid 2%, phenolase 1.5%, polyphenol oxidase 1.5%, catecholoxidase 1.5%, phenylalanine It contains 1%, 1.0% of L-tyrosine and other small amounts of enzymes, vitamin cofactors and gelatin.

The physical properties of the bird's nest extract used in the present invention has a specific gravity of 0.980 at 20 ℃, a refractive index of 1.475 at 20 ℃, dry residues account for 45% at 160 ℃, including heavy metals (arsenic, lead, mercury, etc.) Content) is 0.05 ppm or less.

In the mixed whitening composition according to the present invention, the compounding ratio of arbutin or kojic acid as an auxiliary component and sea bird's nest extract as a main component is 1: 0.1 to 10, preferably 1: 1 to 5 by weight.

Arbutin or kojic acid mixed with the bird's nest extract at a compounding ratio as described above may be added to the softened longevity (skin), nourishing longevity (lotion), nutrition cream, massage cream, essence or pack, etc., each formulation It may generally contain 0.01 to 10.0% by weight, preferably 0.1 to 3.0% by weight based on the total weight of.

The tyrosinase inhibitory effect of the mixed whitening composition according to the present invention may be determined by methods described in the literature, such as those of H. S. Mason and T. Nagath. [Reference: H.S. Mason, Biochem. Biophysics. Acta. 111, 134 (1965); T. Nagath, Experimentia, 28 (6), 634 (1972)].

The present invention will be described with reference to the following examples, but the present invention is not limited thereto, and various embodiments and changes can be made without departing from the spirit and scope of the present invention.

EXAMPLE

Composition Example 1: Nourishing Cream

Using the above ingredients to prepare the desired nutrition cream according to the conventional method in the cosmetic manufacturing field.

Composition Example 2: Nutrients (Lotion)

Using the above ingredients to prepare the desired nutrient cosmetics according to a conventional method in the cosmetic manufacturing field.

Composition Example 3: Softening Longevity (Skin)

Using the above ingredients to prepare the desired softening cosmetics according to the conventional method in the cosmetic manufacturing field.

Composition Example 4: Essence

The above ingredients were used to prepare the desired essence according to conventional methods in the field of cosmetic manufacture.

Composition Example 5: Massage Cream

Using the above ingredients to prepare the desired massage cream according to a conventional method in the cosmetic manufacturing field.

Composition Example 6: Pack

Using the above ingredients, the desired pack was prepared according to a conventional method in the cosmetic manufacturing field.

Performance Evaluation Test

1: Inhibitory Effect of Combination of Swallowweed Extract and Arbutin on Tyrosinase

Tyrosinase activity inhibition experiment of the mixed whitening composition of the present invention was carried out according to the method of Mason (H.S. Mason) and Nagath (T. Nagath). As the test whitening agent, the first group used a combination composition in which the concentration of arbutin was fixed at 0.03% and the concentration of sea bird's nest extract was changed to 0, 0.01, 0.03, 0.1 and 0.3%, and the second group was the concentration of sea bird's nest extract. Was fixed at 0.03% and the concentration of arbutin was changed to 0, 0.01, 0.03, 0.1 and 0.3%. In addition, the third group used only the sea bird's nest extract at concentrations of 0, 0.01, 0.03, 0.1, and 1.0%, and the fourth group used only arbutin at a concentration of 0, 0.01, 0.03, 0.1, and 1.0%. . 1 ml of each said composition was extract | collected, and it was set as the sample. To each of the prepared whitening agent samples, 0.1 ml of tyrosinase solution (5 mg of tyrosinase (3400 units / solid mg) dissolved in 10 ml of 1/15 M phosphate buffer) and 0.9 ml of 1/15 M phosphate buffer (pH 6.8) were added thereto. The mixture was preheated at 37 ° C. for 10 minutes, and then 1 ml of 0.03% L-DOPA (L-3,4-dihydroxyphenylalanine) solution was added thereto, and the mixture was reacted at 37 ° C. for 10 minutes. At this time, the control group was added 1 ml of 1 / 15M phosphate buffer (pH 6.8) instead of each whitening agent sample. After the reaction, the absorbance of the reaction mixture was measured at 470 nm using an absorption spectrometer (UVT06856, MICROPLATE READER from MOLECULAR DEVICE). The tyrosinase activity inhibition rate of each whitening sample was calculated by the following formula.

The results are shown in Tables 1 and 2 below.

Table 1: Tyrosinase Inhibitory Activity According to the Concentration of Sea Swallow Extract and Arbutin

Note) The above figures are expressed as mean ± standard deviation.

Table 2: Tyrosinase Inhibitory Activity According to the Concentration of Sea Bird Extract or Arbutin

Note) The above figures are expressed as mean ± standard deviation.

1A shows that the concentration of arbutin was fixed at 0.03% and the concentration of seabird extract was changed to 0-0.3%, and the concentration of arthropod extract was fixed at 0.03% and the concentration of arbutin was changed to 0-0.3%. The tyrosinase activity inhibition rate of the second group was shown as a graph. In addition, Figure 1B graphically shows the inhibition rate of tyrosinase activity of the third group in which only the seaweed collection extract was changed to 0 to 1.0% concentration and the fourth group in which only arbutin was changed to 0 to 1.0% concentration.

As shown in FIGS. 1A and 1B, the tyrosinase inhibitory effect of the first group and the second group using the combination of arbutin and the bird's nest extract is the third group and the fourth group using the bird's nest extract or arbutin alone. It can be seen that it is greater than the tyrosinase inhibitory effect. In addition, the effect of excessive use of a single component (e.g., concentration of 1.0% in group 3 or 4) and a small mixture of two components (e.g., group 1 or 2) As a result of comparing the effects of the mixed concentration in the group (0.13 to 0.33%), the effects of both are almost similar. Therefore, even when a small amount of the whitening composition mixed with arbutin according to the present invention can be seen that the tyrosinase inhibitory effect is excellent.

2: Inhibitory Effect of Combination of Swallowweed Extract and Kojic Acid on Tyrosinase

Except for using kojic acid in place of arbutin was carried out in the same manner as in the performance evaluation test 1. That is, as the test whitening agent, the first group uses a compound composition in which the concentration of kojic acid is fixed to 0.03% and the concentration of sea bird's nest extract is changed to 0, 0.01, 0.03, 0.1, and 0.3%. A combination composition was used in which the concentration of the extract was fixed at 0.03% and the concentration of kojic acid was changed to 0, 0.01, 0.03, 0.1 and 0.3%. In addition, the third group used only the sea bird's nest extract at concentrations of 0, 0.01, 0.03, 0.1, and 1.0%, and the fourth group used only arbutin at a concentration of 0, 0.01, 0.03, 0.1, and 1.0%. .

The results are shown in Tables 3 and 4 below.

Table 3: Tyrosinase Inhibitory Activity According to the Concentration of Sea Swallow Extract and Koji Acid

Note) The above figures are expressed as mean ± standard deviation.

Table 4: Tyrosinase Inhibitory Activity According to Concentration of Sea Swallow Extract or Kojic Acid

Note) The above figures are expressed as mean ± standard deviation.

In FIG. 2A, the concentration of kojic acid is fixed at 0.03%, the concentration of the first group and the bird's nest extract is changed to 0.03%, and the concentration of kojic acid is 0 to 0.3%. The tyrosinase activity inhibition rate of the second group changed to 0.3% is shown graphically. In addition, in FIG. 2B, the tyrosinase activity inhibition rate of the third group in which only the seaweed collection extract was changed to 0-1.0% concentration and the fourth group in which only koji acid was changed to 0-1.0% concentration are shown in the graph.

As shown in FIG. 2A and FIG. 2B, the tyrosinase inhibitory effect of the first group and the second group using the combination of kojic acid and the bird's nest extract is the third group and the fourth group using the bird's nest extract or koji acid alone. It can be seen that the greater than the tyrosinase inhibitory effect of. In addition, the effect of excessive use of a single component (e.g., concentration of 1.0% in group 3 or 4) and a small mixture of two components (e.g., group 1 or 2) As a result of comparing the effects of the mixed concentration in the group (0.13 to 0.33%), the effects of both are almost similar. Therefore, it can be seen that the tyrosinase inhibitory effect is excellent even when a small amount of the whitening composition mixed with kojic acid according to the present invention is used.

As can be seen from the results of the above performance evaluation test, the mixed whitening composition of the present invention is compared with the whitening effect on tyrosinase inhibitory action and melanin pigmentation inhibitory action obtained when the conventional arbutin or kojic acid is used alone, Excellent whitening effect and excellent whitening effect can be obtained even in small amount.

Claims (4)

A mixed whitening composition comprising, as a main component, a bird's nest extract extracted from a bird's nest, and containing arbutin or kojic acid as a secondary component. The mixed whitening composition according to claim 1, wherein the mixing ratio of the arbutin and the bird's nest extract is 1: 0.1 to 10% by weight. The mixed whitening composition according to claim 1, wherein the mixing ratio of the kojic acid and the bird's nest extract is 1: 0.1 to 10% by weight. The mixed whitening composition according to any one of claims 1 to 3, wherein a blend of any one of the auxiliary components and the seaweed extract is contained in an amount of 0.01 to 10% by weight based on the total weight of the composition.

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