KR100399720B1 - Composition containing extract derived from natural products that have growth-inhibition activity against dandruff causing microorganism - Google Patents

Abstract

The present invention relates to an anti-dandruff composition comprising a plant extract. In particular, the present invention relates to low-toxic natural antibacterial plant extracts, plant essential oils and compounds derived from the plant extracts that inhibit the growth of the pathogenic yeast Pytilosporum obale.

Description

Anti-dandruff composition comprising plant extracts {COMPOSITION CONTAINING EXTRACT DERIVED FROM NATURAL PRODUCTS THAT HAVE GROWTH-INHIBITION ACTIVITY AGAINST DANDRUFF CAUSING MICROORGANISM}

[TECHNICAL FIELD OF THE INVENTION]

The present invention relates to an anti-dandruff composition comprising a plant extract, and more particularly, to an anti-dandruff composition comprising a plant extract having an antimicrobial activity against dandruff, an essential oil and an antibacterial compound derived from the plant extract.

[Private Technology]

Dandruff is a type of seborrhoeic dermatitis in the scalp, which occurs mainly on the face, head, armpits, and genital areas where sebaceous glands are actively active, and occurs immediately after birth or after puberty.

Recently, it has been known that the causative agent of dandruff is yeast, and interest has recently increased due to the discovery of a fungus called yeast Pyrosporum ovale or Malassezia furfur (Kane J. Summerbell RC). Trichophyton, Microsporum, Epidermophyton, and agents of superficialmycoses.1999.In: Murray PR, Barron EJ, Pfaller MA, Tenover FC, Yolken RH (Eds.), Manual of Clinical Microbiology, 7 ^th ed., American Society for Microbiology Press , Washington, DC. Pp. 1275-1294).

Pytirosporum obale and Malassezia puffer are normal fungi in the scalp (Roberts SOB. 1969. Pityrosporum orbiculare : incidence and distribution in clinically normal skin.British Journal of Dermatology 81: 246). It is found in about the same number in people who do not. It is believed that dandruff is caused by differences in susceptibility to dandruff (Bergbrant IM. 1995. Seborrhoeic dermatitis and Pityrospotum yeasts. Current Topics in Medical Mycology 6: 95-112).

As anti-dandruff drugs, new antimicrobials have been developed in the past decades and appear in the world market. Ketoconazole has proved to be very effective in the treatment of pityriasis versicolor or dandruff (Rausch LJ, Jacobs PH. 1984. Cutis 34: 470-471; Hay RJ, Midgeley G. 1984. Clinical and Experimental Dermatology 9: 571-573; Savin RC. 1984. Journal of the American Academy of Dermatology 10: 824-830).

Although ketoconazole is currently used as an active ingredient in dandruff treatments such as nizoral, there is a problem that can cause adverse effects on the endocrine system if ketoconazole is used for a long time (Schuermeyer T, Nieschlag E. 1982. Ketoconazoleinduced drop in serum and saliva) testosterone.Lancet 1098p; Janssen PAJ, Symoens JE. 1983. American Journal of Medicine 74: 80-85)

In addition, itraconazole was newly synthesized through research to develop an antibacterial agent that is safer than a ketoconazole but is safer for the human body. Ytriazole derivatives have been found to be very effective against many pathogenic fungi associated with humans and have high affinity for keratinous tissues (Van Cutsem J, Van Gerven F, Zaman R, Heeres J, Hanssen PAJ. 1983. Pharmacological and preclinical results with a new oral and topical broad spectrum antifungal R 51211. Proceedings of the 13 ^th International Congress of Chemotherapy, Vienna, Part 40: 1-11). Itraconazole inhibits ergosterol synthesis and acts as a mechanism of binding to P450, a fungal oxidase system that causes membrane function and membrane permeability abnormalities (Grant SM, Clissold SP. 1989. Drugs 37: 310-344).

However, because itraconazole is not transported through sweat glands like ketoronazole, it has a disadvantage in that it is a little slower when taken with oral medication, and it is reported that at least 1,000 mg of prescription is required daily to prevent satisfactory treatment and recurrence. (Grant SM, Clissold SP. 1989. Drugs 37: 310-344). In addition, the majority of patients are prescribed several kinds of drugs at the same time, but the administration of the combination may cause problems with the toxicity of the liver cells and the immune system, which may increase the efficiency or adverse effects of one drug ( Svejgaard E. 1995. Side effects and interactions of oral antifungals.Journal of the European Academy of Dermatology and Venereology 5, page S11; Bickers DR. 1994. Journal of the American Academy of Dermatology 31: S87-S90), with headaches or nausea. It also causes the same symptoms (Georgiou S, Monastirli A, Pasmatzi E, Zografakis C, Tsambaos D. 1997. Oral itraconazole therapy for pityriasis versicolor. Journal of the European Academy of Dermatology and Venereology 8: 203-207).

On the other hand, the ingredients mainly used in anti-dandruff shampoo or lotion are selenium sulfide or zinc pyrithion.

Dandruff is not a shortened or serious illness, but it is a psychological stress for most people. Methods and ingredients to prevent or treat such dandruff are continuously being studied, but the results of studies on ingredients that can be ecologically friendly, harmless to the environment and human beings, and bring about a reduction in the number of dandruff bacteria are still inadequate.

Therefore, an object of the present invention is to provide a nontoxic plant extract having excellent antibacterial activity against fungi.

It is also an object of the present invention to provide a nontoxic plant extract having excellent antibacterial activity against dandruff.

It is another object of the present invention to provide a plant-derived compound that has antibacterial activity against dandruff, is simple to manufacture, and harmless to humans.

In order to achieve the above object, the present invention provides a juniperus oxedrus, Eletaria Serdamomum,Juniper's Communis, Tagetes Glandolipera, Anthemis Nobilis, Korean Drum Sativa, Symbogongon Narders, Salvia Offinalis, Pogostemon Patchouli, Citrus Bergamia, Pelargonium Graveolens, Citrus Aurantifolia, Citrus Paradish, Salvia Scleria, Pimento Racemosa, Menta Piperita, Origanum Bulgari, Migtus Communis, Eugenia Karyopilata, Eucalyptus Globulus, Cana Odorata, Aniva Rososadora, Citrus Retigulata, Melalyuca Tenipolia, Cedus Atlantica, Eucalyptus Citridora, Artesia Absintia, Boswellia Carteri, Rosemarinus Officinalis, Pipenigrum, Cymbogon Martini, Citrus Aurantium, Timothy Bulgarias, Citrus Reticulata, Abies Alba, Timus Mastina, Cymbogon Citrus, Menta Fulleum, Betty Vera Zijanoides, Citrus Limoneum, Menta Spikata, Eugenia Carrillotta, Capsicum Anum Bur Agulo Island, Zinga Officeinalis, Mystica Fragrance, Ravendula Hybrid, Citrus Aurantium, Citrus Synensis, Cupress Sempervirens, Santalum Alboom, Oxymum Basilicum, Therapeutic Dolaprata Burr Hondé, Citrus Unsiu, Fennel, Broiler, cinnamon, Bodybuilding, Eccentric and It provides an anti-dandruff composition comprising an extract extracted from at least one selected plant from the group consisting of ginger.

The present invention also relates to eugenol, isoeugenol, pullegon, carbachol, beta-tuzaphyllin, thymol, alpha-terpineol, 3-phenylpropioaldehyde, 4-hydroxy benzaldehyde, trans-cinnamaldehyde, salicylate. At least one compound selected from the group consisting of silaldehyde, cinnamic alcohol, cinnamic acid methyl ester, (R)-(+)-beta-citronellol, beta-(+)-citronellol, citral and geraniol It provides an anti-dandruff composition comprising a.

Hereinafter, the present invention will be described in detail.

The inventors of the present invention juniperus oxedrus (Juniperus oxycedrus), Eletaria SerdamomumElettaria cardamomum),Juniper's CommunisJuniperus communis), Karum CarviCarum carvi), Tagetes GlandoliferaTagettes glandulifera), Anthemis NobilisAnthemis nobilis), Korean Drum SativaCoriandrum sativum), Symbogongon Narders (Cymbopogon nardus), Salvia officialisSalvia officinalis), Pogostemon patchouliPogostemon patchouli), Citrus BergamoaCitrus bergamia), Pelargonium Graveolens (Pelargonium graveolens), Citrus AurantifoliaCitrus aurantifolia), Citrus Paradish (Citrus paradisi), Salvia ScleriaSalvia sclerea), Pimento Lacemosa (Pimento racemosa), Menta piperita (Mentha piperita), Origanum Bulgari (Origanum vulgare), MIGUS COMMONISMyrtus communis), Eugenia Karyopilata (Eugenia caryophyllata), Eucalyptus globulus (Eucalyptus globulus), Kana Odorata (Canaa odorata), Aniva RosodoraAniba rosaeodora), Citrus retigulata (Citrus reticulata), Melal yucca tenifolia (Melaleuca tenifolia), Cedus Atlantica (Cedus atlantica), Eucalyptus citrudoraEucalyptus citriodora), Artesia Absinthium (Artemesia absinthium), Boswellia CarteriBoswellia carterii), Rosemarinus OfficinalisRosmarinus officinalis), Pipenigrum (Piper nigrum), Cympogon MartiniCymbopogon martini), Citrus Aurantium (Citrus aurantium), Timothy Bulgarias (Thymus vulgaris), Citrus reticulata (Citrus reticulata), Abies Alba (Abies alba), Timothy Mastisina (Thymus masticina), Cympogon citrus (Cymbopogon citratus), Menta Poole (Mentha pulegium), Betty Vera ZijanoidesVetivera zizanoides), Citrus limonum (Citrus limonum), Menta Spicata (Mentha spicata), Eugenia Carrillo Pilate (Eugenia caryophyllata), Capsicum Anum Bur Agulo IslandCapsicum annuumvar.angulosum), Zinga OfficeisalisZinger officinalis), Mystica Fragrance (Myristica fragrans), Ravendula HybridLavendula hybrida), Citrus Aurantium (Citrus aurantium), Citrus SynenssisCitrus sinensis), Cuprence Sempervirens (Cupressus sempervirens), Santalum Alboom (Santalum album), Oxymum BasilicumOcimum basilicum), Surprise Dolaprata BurhondeThujapsis dolabratavar.hondai), Citrus Unseau (Citrus unshiu), Fennel (Foeniculum vulgare), Broiler (Cinnamomum sieboldii), cinnamon(Cinnamomum cassia), Seshin (Asarum heterotropoides), Eccentric (Gentianopsis barbata) And ginger(Zingiber officinale) To prepare a plant extract, and confirmed the antimicrobial activity of the plant extract. As a result, it was possible to confirm the antimicrobial activity against the dandruff bacteria of the extracts extracted from the plants.

The plant extracts of the present invention are prepared by a conventional plant extraction method, and preferably, the ground products of the plant are mixed with distilled water, alcohol or an organic solvent, and filtered to dry the solvent. The alcohol preferably has 1 to 5 carbon atoms, more preferably ethanol, methanol, propanol or butanol, and most preferably methanol. The organic solvent is preferably hexane or chloroform, most preferably hexane. Extraction sites include flowers, leaves, stems, bark, buds, roots, resins, fruits, seeds, and plants of plants.

In addition, the plant extracts of the present invention can be prepared with essential oils. Plant essential oils can be extracted by a conventional essential oil extraction method, or a commercial oil can be purchased and used. Typical extraction methods of essential oils include distillation using water or steam and compression, and in general, only components having excellent low molecular weight volatility are obtained using steam distillation. The plant essential oil of the present invention is preferably extracted from flowers, leaves, stems, buds, roots, resins, fruits, seeds, plants and the like. The plant essential oils sold can be purchased and used according to the extraction part of the plant. Conventional extraction sites and plant essential oils for each plant are shown below, but are not limited thereto.

The essential oils extracted from the leaves are Timus Bulgari's Thyme white, Tazetes Glandolipera's Tagetes, Cimpopogon Narder's Citronella java, and Salvia Officinalis (Sage), Pogostemon Patchouli's Patchouli, Pimento Lasmososa's Bay, Oregano's Bulgari's Oregano, Mysterus Komunis's Myrtle, Eugene Karyopilata's Cloveleaf, Eucalyptus of Eucalyptus globulus, Tea tree of Melalucaca tenifolia, Lemon eucalyptus of Eucalyptus citrudora, Franzensense of Boswellia Carteri Frankenscence, Petitgrain of Citrus Aurantium, Thyme Red of Timus Bulgari, Marjoram of Timus Masthina, Lemongrass of Cimbopogon Cilatus And Pennyroyal of Menta Fulleium.

The essential oils extracted from the fruit are Cardamone ceylon of Eletaria Cerdamomum, Juniperberry of Junius Komunis, Coriander of Korean Dream Satiboom, and Black Pepper of Pipenigrum. pepper, Pimento berry from Capsicum annum bur Agulosum, Nutmeg from Myristica fragrance and fennel oil.

The essential oil extracted from the seeds is Carum Carby's Caraway.

Essential oils extracted from flowers include Chamomile roman of Anemises Nobilis, Geranium of Pelargonium Graveolens, Clary sage of Salvia Scleria, Peppermint of Menta Piperita (Peppermint), Ylang ylang of Cana Odorata, Tangerine of Citrus Retigulata, Rosemary of Rosemarinus Orignalis, Spearmint of Menta Spicata, Ravendul Hybrida Lavender, basil of oxymum basil, and clove oil of eugenia caryophylla.

Essential oils extracted from the bark are Bergamot of citrus bergamia, Lime dis 5F of citrus aurantifolia, Grapefruits of citrus paradish, Mandarin of citrus reticulata, citrus limo Lemon 10 Fold of Noum, Bitter Orange of Citrus Aurantium, Orange of Citrus Synensis and Citrus of Citrus Unciu.

The essential oils extracted from the trees are Cade oil of Junusus oxedrus, Rosewood of Aniva Rosodora, Cedarwood of Cedus Atlantica, and Wormwood of Artemesia absinthium Wormwood, Cypress of Cuperence Sempervirens and Sandalwood of Santalum Alboom.

The essential oils extracted from the grass are Palmarosa of Cympogon Martini and Vetiver haiti of Betty Vera Zijanoides.

The essential oil extracted from the resin is, the essential oil extracted from the conifer is the Fir needle of the ivy alba, the essential oil extracted from the bud is Clovebud of Caryo pilarta, and the essential oil extracted from the root is the ginger oil Lees's Ginger, Sessin's Sessin Oil, Hemisphere's Cystic Oil and Ginger's Ginger Oil. Essential oils extracted from the bark are cinnamon cinnamon and broiler oil.

The plant extracts of the present invention have antifungal activity against dandruff, and particularly have antiproliferative and growth inhibitory activity against phytirosporium obale known as dandruff.

In addition, in the present invention, the low molecular weight compounds contained in a large amount in the plant extracts were selected, and the antibacterial activity of each of the compounds was observed, and the compounds having the dandruff antibacterial activity were identified.

Antimicrobial compounds having an activity against dandruff of the present invention are eugenol, isoeugenol, pulegone, carvacrol, beta-tujapilcine (β-thujaplicine), thymol ( thymol), alpha-terpineol (α-terpineol), 3- phenyl propionic aldehyde (3-phenylpropionaldehyde), 4- hydroxybenzaldehyde (4-hydroxy bezaldehyde), trans- Shinan aldehyde (trans -cinnamaldehyde), salicylate Aldehyde (salicylaldehyde), cinnanam alcohol, cinnamic acid methyl ester, (R)-(+)-beta-citronellol ((R)-(+)-β-citronellol), Beta-(+)-citronellol (β-(+)-citronellol), citral, ( + ) -limonene ((±) -limonene), alpha-pinene, (+) Beta-pinene ((+)-β-pinene), (s)-(-)-limonene ((s)-(-)-limonene), (-)-menthol ((-)-menthol), gerani Geraniol and linalool. In particular, eugenol and isoeugenol are in clove extract, pullegon is in Korean drum sativaum, carbacrol, beta-tuzapilysin and thymol in Nahanbaek (Thermossis Dolaprata Burhonde), trans-cinnamaldehyde, Salicylate and cinnamic alcohol are representative compounds contained in broilers and cinnamon. The antimicrobial compounds may be purchased from commercially available compounds, and may be separated and purified from the plant essential oils or plant extracts through known techniques.

The present invention also provides an anti-dandruff bacterium comprising a plant extract or an antibacterial compound. The plant extract is juniperus oxysedrus, Eletaria Serdamomum,Juniper's Communis, Karum Carvi, Tagetes Glandolipera, Anthemis Nobilis, Korean Drum Sativa, Symbogongon Narders, Salvia Offinalis, Pogostemon Patchouli,0Citrus Bergamia, Pelargonium Graveolens, Citrus Aurantifolia, Citrus Paradish, Salvia Scleria, Pimento Racemosa, Menta Piperita, Origanum Bulgari, Migtus Communis, Eugenia Karyopilata, Eucalyptus Globulus, Cana Odorata, Aniva Rososadora, Citrus Retigulata, Melalyuca Tenipolia, Cedus Atlantica, Eucalyptus Citridora, Artesia Absintia, Boswellia Carteri, Rosemarinus Officinalis, Pipenigrum, Cymbogon Martini, Citrus Aurantium, Timothy Bulgarias, Citrus Reticulata, Abies Alba, Timus Mastina, Cymbogon Citrus, Menta Fulleum, Betty Vera Zijanoides, Citrus Limoneum, Menta Spikata, Eugenia Carrillotta, Capsicum Anum Bur Agulo Island, Zinga Officeinalis, Mystica Fragrance, Ravendula Hybrid, Citrus Aurantium, Citrus Synensis, Cupress Sempervirens, Santalum Alboom, Oxymum Basilicum, Therapeutic Dolaprata Burr Hondé, Citrus Unsiu, Fennel, Broiler, cinnamon, Bodybuilding, Eccentric and It is an extract extracted from plants selected from one or more species from the group consisting of ginger. The plant extract is an extract extracted with distilled water, alcohol or an organic solvent, or an essential oil.

The anti-dandruff composition of the present invention has growth inhibitory activity against yeast or pathogenic fungi existing on the outer shell, and preferably has growth inhibitory activity against dandruff.

The anti-dandruff composition can be used for inhibiting the growth of pathogenic fungi or yeasts, and especially can be used for the prevention, treatment and disinfection of dermatitis. The antidandruff composition may be prepared as a detergent, a medicament or a cosmetic. Examples of the cleaning agent include shampoo, rinse, soap, and the like. There are disinfectants, therapeutic agents, and preventive agents, and cosmetics include lotions, skins, creams, and essences. In case of using the anti-dandruff composition as a medicament, possible formulations include warning agents, granules, lotions, liniments, limonades and fragrances. Iii) powders, syrups, liquids, aerosols, extracts, elixiers, ointments, liquid extracts, Emulsions, Suspensions, Acupuncture Agents, Premises, Eye Drops, Tablets, Suppositories, Injections, Alcohols, Cataplasma ), Capsules, creams, troches, tinctures, pasta or pills.

The anti-dandruff composition of the present invention preferably comprises 0.1 to 20% by weight of plant extracts or essential oils, but is not limited thereto. In addition, the anti-dandruff composition may further include one or more acceptable pharmacological compositions depending on the formulation and the method of use. Pharmacological compositions are excipients or diluents, for example saline, buffered saline, dextrose, water, glycerol and ethanol.

Hereinafter, examples of the present invention will be described. The following examples are only for illustrating the present invention and the present invention is not limited to the following examples.

Example 1: Plant Extract Preparation

Buds of cinnamon, broilers, hemispheres and cloves were purchased through Boeun Herbal Medicine in Gyeongdong Market, Seoul, and methanol extracts were obtained for each. All the methanol extract manufacturing method was carried out in the same manner, as an example showed a methanol extraction method of cinnamon.

Grind 300 g of cinnamon finely with a blender, place in a 500 ml Erlenmeyer flask, mix 200 ml of methanol, leave for 2 days at room temperature in the dark, and filter the resulting filtrate under reduced pressure (EYELA autojack NAJ-160, Japan). Concentrated. The filtrate filtered through filter paper under reduced pressure was concentrated again in the same manner by adding 200 ml of methanol to finally prepare a methanol extract.

Example 2: Preparation of Plant Essential Oils

Plant essential oils described in Table 1 below were purchased.

Refined name Botanical name The name extraction One Thyme white Timus Bulgari Lamiaceae leaf 2 Ked oil Juniperus oxedrus Cypressaceae wood 3 Cardamom Seilon Eletaria Serdamomum Ginger fruit 4 Juniper berry Juniper's Communis Cypressaceae berries 5 Caraway Seed Karum Carvi Morphology seed 6 Tagetes Tazetes Glandolipera Chrysanthemum leaf (herb) 7 Chamomile Roman Anthemis Nobilis Chrysanthemum flower 8 Coriander Korean Room Sativaboom Morphology fruit 9 Citronella java Symbogongon Narders Rice plant Leaf 10 sage Salvia Officinalis Lamiaceae leaf (herb) 11 Patchouli Pogostemon Patchouli Lamiaceae leaf 12 Bergamot Citrus Bergamia Rhyme peel 13 Geranium Pelargonium Graveolens Mouse grass flower (herb) 14 Lime Discover 5F Citrus Aurantifolia Rhyme peel 15 Grapefruit Citrus Paradish Rhyme peel 16 Claire sage Salvia Scleria Lamiaceae flower

17 Bay Pimento Lacemosa Plating quantity leaf 18 Peppermint Menta Piperita Lamiaceae flower (herb) 19 oregano Origanum Bulgari Lamiaceae leaf 20 Myrtle MIGUS COMMONIS Plating quantity leaf 21 Cloverlip Eugenia Karyopilata Ash Tree leaf 22 Eucalyptus Eucalyptus Globulus Plating quantity leaf 23 Ylang-ylang Kana Odorata King tree flower 24 Rosewood Aniva Rossodora Camphoraceae wood 25 Tangerine Citrus Retigulata Rhyme flower 26 Tea tree Melalyuca Tenipolia Plating quantity leaf 27 Cedar Wood Cedus Atlantica Cypressaceae wood 28 Lemon Eucalyptus Eucalyptus Citridora Plating quantity leaf stem 29 Warmwood Artesia Absinthium Chrysanthemum wood 30 Frankensen Boswellia Carteri Sumac leaf 31 Rosemary Rosemarinus Orignalis Lamiaceae flower (herb) 32 Black Pepper Fife Nigrum Peppercorns fruit 33 Palmarosa Cymbogon Martini Rice plant grass 34 Pettigrain Citrus Aurantium Rhyme leaf (twig) 35 Thyme red Timus Bulgari Lamiaceae leaf (herb) 36 Mandarin Citrus Reticulata Rhyme peel 37 Funny Avies Alba Pine tree cone 38 Marjoram Timus Mastina Lamiaceae leaf (herb) 39 Lemongrass Cympogon Cilatus Rice plant leaf 40 Phenyl Royal Menta Fulleium Lamiaceae leaf 41 Vetiver Haiti Betivera Zijanoides Rice plant grass 42 Lemon 10Fold Citrus limonum Rhyme peel 43 Spearmint Menta Spikata Lamiaceae flower, leaf 44 Clove Bird Eugenia Caryopilata Ash Tree bud 45 Pimento Berry Capsicum Anum Bur Agulo Island Eggplant Fruit 46 Jinger Zinger Offisinalis Ginger root 47 Nut Mug Mystica Fragrance Nutmeg fruit 48 lavender Ravendula Hybrid Lamiaceae flower 49 Bitter Orange Citrus Aurantium Rhyme peel 50 Orange Citrus sinensis Rhyme peel 51 Cypress Cupress Sempervirens Cypressaceae twig 52 Sandalwood Santalum Alboom Unidirectional wood 53 basil Oxymum Basilicum Lamiaceae flower (herb) 54 Cinnamon Shin Nam Om Cassia Camphoraceae flower (herb) 55 Khiva Tujasys Dolabrata Bur, Hornday Cypressaceae sawdust 56 Citrus Citrus Unsiu Rhyme peel 57 fennel Penny Column Bulgari Pictograph fruit 58 Broiler Sinnam Brothers Sieboldi Camphoraceae bark 59 Sesin Asarum Heterotrophoides Rat Vine and root 60 Eccentric Gentianopsis Barbata Gentian root 61 ginger Genghis Office Ginger root

Cinnamon was purchased or supplied from Sigma Aldrich and Hiba from Japan Institute of Solar Chemical Research. The essential oil is prepared by steam distillation.

As an example, an essential oil manufacturing method is shown below.

Or Hanbaek (Thujapsis dolabrata var. Hondai) sawdust, Cinnamon (C. cassia) leaf and tangerine (Citrus unshiu) menteul of the flask was then heated over menteul (heating mentle) placed in a 3 L flask and concentrated by finely grinding the shell into each mixer The temperature was adjusted to about 70 ° C and the hexanes were mixed and heated for 3 hours. The amount of sample required for distillation once was 250 g, and after extraction, a small amount of anhydrous sodium sulfate (Na ₂ SO ₄ ) was added to remove moisture contained in the extract, and then left in a desiccator. 2) and concentrated.

Example 3: Determination of antimicrobial activity of plant extracts

One species of Pityrosporum ovale KCCM 11894 (ATCC 12078) was distributed at the Korean Culture Center of Microorganisms.

Growth inhibition was tested by the paper disc diffusion method, which is used to compare or test the antimicrobial activity against Pytirosporum obale (Squiquera L, Plotkin L, Mathov I, Galimberti R). , Leoni J. 1996. Analysis of the antifungal activity of ketoconazole, zinc pyrithione, and ciclopirox olamine against Pityrosporum ovale.A diffusionassay for cultures in solid media.Journal of the European Academy of Dermatology and Venereology 7: 26-29).

60.0 g of Malt Extract Agar, phytirosporum obale, 20.0 g of Ox-bile (desicated), 10.0 g of Tween40, 2.5 g of Glycerol mono-oleate, 1.0 of distilled water The medium containing L was inoculated at a concentration of 10 ⁵ -10 ⁶ CFU / ml.

Each methanol extract (Example 1), essential oil (Example 2) or antibacterial compound was dissolved in ethanol and treated with 50 ul around 8 mm in diameter and the solvent was volatilized in the hood for 5 minutes. Each treated place was placed on a medium inoculated with phytirosporum obale and exhaled incubation at 28 ° C. for 3 days.

The same experiment was performed by dissolving itraconazole in chloroform as a control. Table 2 shows the growth inhibition ring size by the methanol extract and essential oils. Both methanol extract and essential oil showed inhibitory activity against Pytilosporum obale. Table 20 shows only those showing growth inhibition of 12 mm or more when 20 mg of them were treated.

Inhibitory ring, mm 20 mg 10 mg 5 mg 2.5 mg 1.25 mg Remarks Thyme white 18 18 14 8 8 Ked oil 14 13 8 8 8 Caraway Seed 18 13 12 8 8 Coriander 33 15 9 8 8 growth Citronella java 40 8 8 8 8 growth Geranium 18 15 14 14 10 Lime Discover 5F 23 22 8 8 8 Claire sage 13 11 10 9 8 Bay 20 17 15 12 8 Peppermint 25 17 16 8 8 Cloverlip 18 17 16 13 10 Rosewood 25 23 23 15 8 growth Tea tree 23 12 11 8 8 Lemon Eucalyptus 20 12 11 8 8 growth Warmwood 15 8 8 8 8 Frankensen 12 12 9 8 8 Palmarosa 25 25 25 18 15 Pettigrain 12 10 9 8 8 Thyme red 18 18 14 10 8 Lemongrass 45 45 30 20 16 growth Penin Royal 25 22 10 9 8 Lemon 10Fold 43 12 12 11 8 growth Spearmint 24 12 10 8 8 Clove Bird 18 18 16 16 11 Pimento Berry 17 14 14 12 8 Nut Mug 15 12 10 9 8 lavender 15 15 11 8 8 basil 18 12 10 8 8 Khiva 31 25 18 18 16 Eucalyptus 18 14 12 10 8 Citrus 13 11 8 8 8 Cinnamon 45 35 32 30 22 Broiler Methanol Extract 40 28 25 25 16 Cinnamon Methanol Extract 45 33 32 31 22 Sesine Methanol Extract 12 12 12 10 8 Cylindrical Methanol Extract 30 28 20 12 8 Clove Methanol Extract 17 14 14 10 8 Itraconazole 13 13 13 12 12 ethanol - - - - - chloroform - - - - -

As shown in Table 2, the phytorosporium obale in plant essential oil extracts were examined after 24 hours of treatment. Time White, Ked Oil, Caraway Seed, Coriander, Citronella Java, Geranium, Lime Dis 5F, Clevis Resage, Bay, Peppermint, Clover Lip, Rosewood, Tea Tree, Lemon Eucalyptus, Warmwood, Franken Sense, Palmarosa, Petigrain, Thyme Red, Lemongrass, Penin Royal, Lemon 10Fold, Spearmint, Clove Bird, Pimento 20 mg of berry, nut mug, lavender, basil, heba, eucalyptus, citrus, cinnamon, broiler methanol extract, cinnamon methanol extract, sesine methanol extract, single-headed methanol extract and clove methanol extract showed strong inhibitory activity Especially coriander, citronella java, lemongrass, lemon 10fold, heba broth methanol extract, cinnamon methanol weight The water and pyeonnoe methanol extract showed more than 30 mm high inhibitory activity.

Essential oils and plant extracts with antimicrobial activity of 15 mm or more at 10 mg treatment level were thyme white, coriander, geranium, lime dis 5F, bay, peppermint, cloblip, rosewood, palmarosa, thyme red, lemongrass, penny royal It is clovebird, lavender, heba, cinnamon, broiler methanol extract, cinnamon methanol extract, and hemisphere methanol extract. At the 5 mg treatment level, all plant essential oils and extracts except Ravendon maintained high growth inhibition activity. At the lowest concentration of 1.25 mg in this experiment, palmarosa, lemongrass, heba, cinnamon, broiler methanol extract and cinnamon methanol extract showed strong antimicrobial activity.

Growth inhibition activity was further confirmed after 3 days after 24 hours. As a result, coriander, citronella java, rosewood, lemon eucalyptus, lemongrass, and lemon 10fold, growth inhibitory activity against dandruff was weakened rapidly, and colony of bacteria was formed in the place where it was initially suppressed, and the growth of the bacteria was recovered. Aspect was observed. This phenomenon inhibits the growth of dandruff at high concentrations, but it is estimated that the bacteria growing around the margin grow gradually due to the evaporation of the active ingredient volatilized over time. Other plant extracts retained their inhibitory activity after one week.

Itraconazole, used as a control, did not show any concentration-dependent pattern of growth inhibition of more than 12 mm at all treatment levels. This result is presumed to be due to the diffusion of itraconazole in the medium.

Example 4: Determination of antimicrobial activity of antimicrobial compounds

Growth inhibition activity of the antimicrobial compounds was measured in the same manner as in Example 3, and the size of growth inhibition ring is shown in Table 3 below.

essential oil Inhibitory ring, mm 5 mg 2.5 mg 1.25 mg 0.625 mg Eugenol 17 13 10 8 Isoeugenol 15 11 9 8 Pullegon 33 8 8 8 Cabatrol 20 15 12 8 Beta-tuzaplicin 45 45 44 40 Thymol 20 15 10 8 Alpha-terpineol 24 15 8 8 3-phenylpropioaldehyde 28 14 9 8 4-hydroxy benzaldehyde 13 8 8 8 Trans-cinnaaldehyde 33 30 27 23 Salicylate 50 50 9 8 Shinnamil Alcohol 16 9 8 8 Cinnamic Acid Methyl Ester 15 10 8 8 (R)-(+)-beta-citronellol 19 18 10 8 Beta-(+)-citronellol 21 14 8 8 Citral 35 22 15 10 (±) -limonene 17 13 9 8 Alpha-Pyrene 13 11 8 8 (+)-Beta-pyrene 14 12 8 8 (s)-(-)-limonene 12 9 8 8 (-)-menthol 18 9 8 8 Geraniol 22 19 14 9 Linalol 12 10 8 8 Itraconazole 12 12 12 12 ethanol - - - - chloroform - - - -

As shown in Table 3 above, compounds showing similar or better activity compared to itraconazole used as a control include eugenol, isoeugenol, pullegon, carbacrol, beta-tuzaplicin, thymol, Alpha-terpineol, 3-phenylpropionaldehyde, 4-hydroxy benzaldehyde, trans-cinnaaldehyde, salicylaldehyde, cinnamic alcohol, cinnamic acid ethyl ester, (R)-(+)-beta-citronellol, beta -(+)-Citronellol, citral, (±) -limonene, α-pinene, (+)-β-pinene, (s)-(-)-limonene, (-)-menthol, geraniol and Linalol, at 2.5 mg eugenol, isoeugenol, carbacroll, beta-tuzaflisin, thymol, alpha terpineol, 3-phenylpropionaldehyde, trans-cinnamaldehyde, salicylaldehyde, cinnamic acid methyl ester, (R)-(+)-beta-citronellol, beta-(+)-citronellol, citral, (±) -limonene, (+)-beta-pinene and geraniol It showed strong antimicrobial activity.

At the lowest treatment level, 0.625 mg, beta-tuzaplicin and trans-cinnamaldehyde showed very high growth inhibitory activity.

The anti-dandruff composition comprising the plant extract or the antimicrobial compound of the present invention exhibits excellent antibacterial activity against dandruff, and can be used as a natural antimicrobial agent as well as to prevent or treat dandruff inhabiting the skin and to have low toxicity.

Claims (5)

(Correction) Timus Bulgari, Juniper Oxy Cedrus, Eletaria Serdamomum,Juniper's Communis, Tagetes Glandolipera, Anthemis Nobilis, Korean Drum Sativa, Symbogongon Narders, Salvia Offinalis, Pogostemon Patchouli, Citrus Bergamia, Pelargonium Graveolens, Citrus Aurantifolia and An anti-dandruff composition comprising an extract extracted from at least one plant selected from the group consisting of citrus paradish. (Correction) The anti-dandruff composition according to claim 1, wherein the extract is extracted using at least one solvent selected from the group consisting of ethanol, methanol, propanol, butanol, hexane, chloroform and distilled water. The anti-dandruff composition according to claim 1, wherein the extract is a plant essential oil. (Correction) The plant essential oil according to claim 3, wherein the plant essential oil is thyme white, thyme red, ked oil, cardamom selon, juniper berry, tagetes, chamomile roman, coriander, citronella java, sage, patchuli, bergamot, geranium , Anti-dandruff composition selected from the group consisting of lime dis 5F and grapefruit. (Correction) eugenol, isoeugenol, pullegon, carbachol, beta-tuzaphyllin, thymol, 3-phenylpropioaldehyde, 4-hydroxy benzaldehyde, trans-cinnamaldehyde, salicylaldehyde, cinnamic alcohol, cinnamic An anti-dandruff composition comprising at least one compound selected from the group consisting of acid methyl ester, (R)-(+)-beta-citronellol, beta-(+)-citronellol, citral and geraniol.