KR100334248B1 - Method of preparing functional crops inoculated by mushroom - Google Patents

Abstract

The present invention relates to a method of increasing the germination rate of cereals, inoculating the seed of the mushrooms to the germinated grains, to produce nutritious grains, and more specifically, by germinating the grains, and then inoculating the seed of the mushrooms to the germinated grains. It is intended to produce functional cereals that increase digestibility and nutritional value and the efficacy of cereals.

Description

Method of preparing functional grains inoculated by mushroom fungi {Method of preparing functional crops inoculated by mushroom}

The present invention relates to a method of increasing the germination rate of cereals, inoculating the seed of the mushrooms in the germinated grains, and producing nutritious grains, and more particularly, after the grains are germinated by treating chitosan, By inoculating spawn, it is intended to produce functional grains that increase digestibility and enhance nutritional value and grain efficacy.

In general, chitin is used in binders, emulsifiers, tubes and the like, and may be used in artificial kidney membranes, biodegradable drug carriers, and the like for medical applications. On the other hand, reports of chitosan used in plants showed that US Patent No. 4,199,496 reported that chitosan could be used as a fertilizer, and that chitosan reduced plant collapse and promoted stem diameter and root growth. . In US Pat. No. 4,964,894 and the like, chitosan used in combination with amino acids is known to promote the germination of effective plant seeds. In addition, chitosan oligosaccharides were coated on the leaves to show antimicrobial activity against the phytopathogenic fungus Fusarium sorani, and the oligosaccharides were coated on the seeds and tested. The chitinase activity required for seed germination was 1.3 times higher than that of untreated seeds. There was also a report to increase. In addition, germinated grains have been reported to increase digestibility.

The inventors of the present invention have developed a method of promoting the germination of grains using chitosan using compounds other than amino acids to increase the digestibility of grains.

Traditionally, mushrooms have been grown on trees. Several improved mushroom cultivation methods have been developed, one of which is the use of cereals in mushroom production. According to the method described in U.S. Patent No. 1,869,517, the dried cereals are placed in a bottle containing a certain amount of water and calcium carbonate, which is closed with a cotton cap, sterilized at 121 ° C for 35 to 45 minutes, cooled and then bottled. Inoculate, close the bottle again and incubate as needed.

However, in this method, when the grain near the bottom of the bottle is sterilized, it is easy to form a lump, so that the inoculation can form mycelia that are difficult to remove from the bottle, and the sterilization period is long, and much of the mycelia is contaminated. There is a problem of destabilization.

The present invention solves the above problems, and the object of the present invention is to provide a method for producing functional cereals to increase the digestive efficiency, nutritional value and the efficacy of cereals.

1 is a schematic diagram of a method for producing functional cereals inoculated with mushroom fungi.

In order to achieve the above object, the present invention comprises the steps of immersing the grains in chitosan solution, germinating the immersed grains, inoculating the seed to the germinated grains and culturing the inoculated grains After inoculating the mushroom fungus to provide a method of producing functional grains through a freeze-drying process.

As said grains, soybeans including brown rice, red beans, yulmu, buckwheat, millet, crude, green beans, and yellow beans are preferable.

As for the spawn to be used for inoculation of the germinated grains, agaricus, matsutake, eggplant, shiitake, zelkova, Cordyceps sinensis, ganoderma lucidum, leafy bird, spiritual mushroom, etc. are preferable.

Above The concentration of the chitosan solution is not particularly effective at 300 ppm or less, and 300 to 3000 ppm is preferable since the chitosan solution has no effect.

When the chitosan solution is prepared, it is preferable to dissolve chitosan using a solvent containing herbal medicine extracts including green tea, duchung tea, and ginseng. In the above, since it did not show the difference of germination rate, and there is no economical efficiency, 0.01%-0.1% are preferable.

As for said immersion temperature and time, 5 to 20 hours are preferable at 10-25 degreeC.

Germination conditions were 0.001-0.1% green tea extract containing 300-3,000 ppm chitosan as a solvent, soaked at 18-20 ° C for 5-15 hours, and then germinated for 1 to 3 hours while spraying the solution at 15-25 ° C. In the present invention, 0.01 to 0.05% green tea extract containing 300 to 500 ppm chitosan was used as a solvent, and then immersed at 18 to 20 ° C. for 7 to 10 hours, and then sprayed with the solution at 18 to 20 ° C. It is suitable to germinate for -3 days.

The conditions for culturing the inoculated grains are sterilized by the germinated grains, it is preferable to incubate for 7 to 20 days at 18-30 ℃, in the present invention, after sterilizing germinated grains at 110 ℃ 30 minutes in an autoclave, It is appropriate to incubate for 10 to 15 days at 20 to 25 ℃.

Chitosan is a derivative of chitin, which is a polysaccharide composed mainly of β- (1-4) -2-acetamido-2-dioxy-D-glucose (N-acetyl-D-glucosamine) residues. Chitin is found a lot in nature. It is found in arthropods such as crustaceans, insects and spiders, and mollusks such as squids, and also in various fungal species.

In shellfish and the like, the shell consists of a protein chemically bound to chitin mixed with calcium carbonate. To separate the chitin from the protein, the shell is treated with an aqueous alkaline solution to break the bond between the protein and the chitin. Then, in order to separate chitin from calcium carbonate, calcium carbonate is removed and an acid such as hydrochloric acid is treated to obtain pure chitin.

Desalted chitin is washed on a rotary vacuum filter and dried in a rotary warm air dryer. The chitin is then converted to chitosan, one of its derivatives.

Hereinafter, the present invention will be described in detail through non-limiting comparative examples and examples.

Example 1

Example 1-1

To determine the effect of chitosan on the germination of cereals, especially brown rice, brown rice was immersed at 17 ° C for 10 hours in a solution made of 300 ppm of oligo chitosan dissolved in 0.005% citric acid solution, and then dried. Germination was carried out for 6 to 7 hours while spraying the chitosan solution while maintaining the temperature at 20 ° C. so as not to dry the brown rice.

Example 1-2

The solution of 3,000 ppm of oligo chitosan dissolved in 0.005% citric acid solution was immersed for 10 hours at 17 ° C, and then drained to keep the germination room temperature at 20 ° C while spraying the chitosan solution to prevent the brown rice from drying. Germination was carried out for 7 hours.

Comparative Example 1

Brown rice was immersed in 0.005% citric acid solution at 17 ° C. for 10 hours and then taken out, while germination was carried out for 6 to 7 hours while spraying chitosan solution to keep the germination chamber temperature at 20 ° C.

As a result of germination test, the germination rate of oligo chitosan immersion solution of 300ppm and 3,000ppm was as high as 95%, compared with 68% of control. In addition, the decay generated during immersion was also greatly improved.

[Table 1] Germination rate and rot according to chitosan concentration

Comparative Example 1 Example 1-1 Example 1-2 Germination rate 68% 94% 95% Corruption Severe Very improved Significantly improved

Example 2

The germination rate of the green tea extract of 0.01% as a solvent and the oligo chitosan solution as 0, 300 and 3000ppm as in Comparative Example 1 and Example 1 was investigated, and as shown in Table 2, the germination rate and decay odor were greatly improved.

This is thought to be due to the flavonoid component and the polyphenol component in green tea along with the effect of chitosan.

[Table 2] Germination rate and decay when preparing chitosan solution using green tea extract as a solvent

Chitosan concentration 0 300 ppm 3,000 ppm Germination rate 70% 96% 98% Corruption Slightly improved Very improved Significantly improved

Example 3

Example 3-1

The grains germinated by Examples 1 and 2 were directly inoculated with the seed of Agaricus and incubated at 20 to 25 ° C. for 10 to 20 days, resulting in evenly spreading mushroom grains.

Example 3-2

The grains germinated by Examples 1 and 2 were directly inoculated with seed seeds of the cluster and incubated at 20 to 25 ° C. for 10 to 20 days, resulting in evenly spreading mushroom grains.

Example 3-3

The grains germinated by Examples 1 and 2 were directly inoculated with the seed of the situation and incubated at 20 to 25 ° C. for 10 to 20 days. As a result, mushroom grains with evenly spread mycelia were produced.

Example 3-4

The grains germinated by Examples 1 and 2 were directly inoculated with the seeds of shiitake and incubated at 20 to 25 ° C. for 10 to 20 days. As a result, mushroom grains with evenly spread mycelia were produced.

Example 3-5

The grains germinated by Examples 1 and 2 were directly inoculated with seedlings of zelkova and incubated at 20 to 25 ° C. for 10 to 20 days, resulting in evenly spreading mushroom grains.

Example 3-6

The grains germinated by Examples 1 and 2 were directly inoculated with the seeds of Cordyceps sinensis and incubated at 20 to 25 ° C. for 10 to 20 days, resulting in evenly spreading mushroom grains.

Example 3-7

The grains germinated by Examples 1 and 2 were directly inoculated with seedlings of Ganoderma lucidum and incubated at 20 to 25 ° C. for 10 to 20 days, resulting in evenly spreading mushroom grains.

Example 3-8

The grains germinated by Examples 1 and 2 were directly inoculated with seedlings of leaf mushrooms and incubated at 20 to 25 ° C. for 10 to 20 days, resulting in evenly spreading mushroom grains.

Example 3-9

The grains germinated by Examples 1 and 2 were directly inoculated with the spawn of the young mushrooms and incubated at 20 to 25 ° C. for 10 to 20 days to produce mushroom grains evenly spread over the mycelia.

Example 4

The mushroom grains obtained in Example 3 were lyophilized.

According to the present invention having the above-described configuration, the germination rate of the grains treated with chitosan is increased by about 40% to produce a functional grain having a high nutritional absorption and mushroom flavor as well as a high digestive absorption rate.

Claims (7)

a) dipping cereals in chitosan solution; b) germinating the soaked cereals; c) inoculating mushroom spawn to grains germinated by constant culture conditions; d) culturing the inoculated grains; And e) a method of producing functional grains inoculated with mushroom fungi comprising the step of lyophilizing the cultured grains. [Claim 2] The method according to claim 1, wherein the chitosan solution is prepared by dissolving chitosan in a solvent containing an extract of green tea, chungchung tea or ginseng. The method of claim 2, wherein the concentration of the extract is 0.001 to 0.05% by weight inoculated mushroom fungi production method. The method of producing functional cereals inoculated with mushroom fungi according to claim 1, wherein the soaking conditions are 10 to 25 DEG C and 5 to 15 hours. The method for producing functional grains according to claim 1, wherein the culture conditions of the germinated grains are inoculated with mushroom fungi which is 7 to 20 days at 18 to 30 ° C. The method according to claim 1, wherein the grains are brown rice, red beans, barley, buckwheat, millet, crude, mung beans, and yellow beans. The method of claim 1, wherein the mushroom is agaricus, matsutake, circumference, shiitake, zelkova, Cordyceps sinensis, ganoderma lucidum, leafy, new mushrooms.