KR20190143659A - Composition for controlling plant storage disease comprising tobacco root extract and the manufacturing method of the same - Google Patents

Abstract

The present invention relates to a composition for controlling a plant storage disease, which comprises a tobacco root extract as an active ingredient, and to a manufacturing method thereof. The composition of the present invention has anti-bacterial activity against Aspergillus sp. bacteria and Penicillium sp. bacteria.

Description

Composition for controlling plant storage disease comprising tobacco root extract and the manufacturing method of the same}

The present invention relates to a composition for controlling plant storage bottles containing a tobacco root extract as an active ingredient having an antimicrobial activity against Aspergillus and Penicillium sp.

"Plant storage disease" (plant storage disease) is a disease that occurs after the harvest of stored agricultural products, if the temperature and humidity are not controlled during storage to promote the growth of pathogens, the crops after harvesting will be greatly damaged. In general, diseases occurring during transportation after harvesting are transport diseases, markets and retail stores that produce agricultural products such as market diseases, and market diseases among diseases caused by vegetables and fruits are called fruit and vegetable diseases. Currently, domestic horticultural crops amount to 11 trillion won, but damages to storage bottles are serious enough to cause losses of about 3 trillion won during the distribution after harvest. The use of chemical synthetic pesticides in the control of plant diseases is decreasing worldwide due to the Rio Convention, etc., and it is a low toxicity and eco-friendly method to prevent the corruption of storage fruits due to the need for environmentally friendly plant disease control methods. Development of pesticide control methods is becoming important.

In fruits, diseases caused by Penicillium spp. Are not caused during the growing period of the crop, but are mainly caused by wounds in the process of harvesting, storing, and transporting the crops. 10-30%, and some underdeveloped countries make up more than 30%. In addition, if the bottle occurs during the storage of fruits, such as pears, apples, citrus fruits, and grapes, which have high value added, the merchandise may be greatly degraded, resulting in economic losses.

Aspergillus flavus and A. parasiticus , like Penicillium, are another fungal species belonging to the Eurotia species, with the same evolutionary properties. Aspergillus fungi live in nuts and grains, producing a substance called aflatoxins, which is a potent carcinogenic compound. In addition, fungi that mature blue milk cheese made from milk and Eurotia fungi that cause plant diseases in citrus fruit have been found to fall into the same category. Eurotia fungi of this nature also include pathogenic fungi that cause diseases such as aspergilloses.

Previous studies have shown that natural compounds such as jasmonic acid, amino acids and their derivatives beta-aminobutyl acid (βacid) regulate plant growth in order to improve fruit resistance to plant storage diseases. There have been developed methods to control physical storage bottles such as methods of use, visible light, UV, X-ray, gamma irradiation, heat treatment, and fume treatment, but only a few pesticides are derived from natural products. Pesticides derived from natural products can solve problems such as the emergence of resistant bacteria to chemical pesticides, development costs are low, low toxicity and environmentally friendly advantages, the development of pesticides derived from natural products is constantly being required.

Under these circumstances, the present inventors have made diligent efforts to discover natural substances having plant storage disease control effects, and as a result, tobacco root extracts have been isolated from the genus Aspergillus or Penicillium genus. The present invention was completed by confirming the growth inhibition effect.

It is an object of the present invention to provide a composition for controlling plant storage bottles and a method for preparing the same, comprising the tobacco root extract as an active ingredient.

However, the problem to be solved by the present invention is not limited to the above-mentioned problem, another task that is not mentioned will be clearly understood by those skilled in the art from the following description.

According to an embodiment of the present invention, a composition for controlling plant storage bottles is provided, which contains tobacco root extract as an active ingredient.

According to one side, the plant storage disease may be caused by the genus Aspergillus (Aspergillus) or Penicillium (Penicillium).

According to one side, the genus Aspergillus is Aspergillus niger , the genus Penicillium penicillium Italicum ( Penicilium) italicum ), Penicilium expansum .

According to one side, the tobacco root extract may be extracted from Brazilian Mapocho, Black Rustica or yellow species tobacco.

According to one side, the tobacco root extract may be obtained by using at least one selected from acetone, alcohol, lower alcohol having 1 to 4 carbon atoms, ethyl acetate, and 1,3-butylene glycol as the extraction solvent.

According to one side, the tobacco root extract may be 70% acetone extract of the root of brazilian mapacho, 70% acetone extract of the black rosica root or ethanol extract of the yellow species root.

According to another embodiment of the present invention, there is provided a plant storage bottle control agent comprising a composition according to an embodiment of the present invention.

According to one side, the control agent may be in the form of granules, liquids, hydrates, granules, dispersants or capsules.

According to one side, the control agent may further comprise one or more adjuvants selected from surfactants, pressure-sensitive adhesives, extenders, wetting agents, dispersants and nutrients.

According to another embodiment of the present invention, preparing the roots of yellow species, brazilian mapacho or black Rustica tobacco; Preparing an extract by immersing the root of the tobacco in an extraction solvent; And concentrating the extract by filtration. It includes, a method for producing a composition for controlling plant storage bottles is provided.

According to one side, after the filtration and concentration step, adding at least one adjuvant selected from surfactants, tackifiers, extenders, wetting agents, dispersants and nutrients; It may further include.

According to one side, the extraction solvent may be at least one selected from acetone, alcohol, lower alcohol having 1 to 4 carbon atoms, ethyl acetate and 1,3-butylene glycol.

The composition for controlling plant storage bottles of the present invention exhibits excellent antibacterial activity against Aspergillus or Penicillium bacteria, and contains tobacco root extract as an active ingredient to control plant storage bottles without environmental pollution or human toxicity. can do.

In addition, the production method of the plant storage bottle control composition of the present invention can be mass-produced tobacco root extract in a simple process.

It is to be understood that the effects of the present invention are not limited to the above effects, and include all effects deduced from the configuration of the invention described in the detailed description or claims of the present invention.

1 is Aspergillus niger, Penicillium italicum , Penicillium Photograph showing the growth inhibition of brazilian mappa , black rosica and yellow root extract against expansun .

Hereinafter, exemplary embodiments will be described in detail with reference to the accompanying drawings. However, various changes may be made to the embodiments so that the scope of the patent application is not limited or limited by these embodiments. It is to be understood that all changes, equivalents, and substitutes for the embodiments are included in the scope of rights.

The terminology used herein is for the purpose of description and should not be construed as limiting. Singular expressions include plural expressions unless the context clearly indicates otherwise. In this specification, terms such as "comprise" or "have" are intended to indicate that there is a feature, number, step, action, component, part, or combination thereof described on the specification, and one or more other features. It is to be understood that the present invention does not exclude the possibility of the presence or the addition of numbers, steps, operations, components, components, or a combination thereof.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art. Terms such as those defined in the commonly used dictionaries should be construed as having meanings consistent with the meanings in the context of the related art and shall not be construed in ideal or excessively formal meanings unless expressly defined in this application. Do not.

In addition, in the description with reference to the accompanying drawings, the same components regardless of reference numerals will be given the same reference numerals and duplicate description thereof will be omitted. In the following description of the embodiment, when it is determined that the detailed description of the related known technology may unnecessarily obscure the gist of the embodiment, the detailed description thereof will be omitted.

As used herein, the term "storage disease" means that when storing crops, fruits, bulbs, vegetables, etc., if the temperature and humidity are not controlled to promote the growth of pathogens, the crops after harvesting are severely damaged. Means the bottle. In general, the diseases occurring during transport after harvest are transported from the distribution process of agricultural products such as transit disease, market and retail stores. (靑 果) is called a disease.

As used herein, the term "control" refers to the prevention, treatment or alleviation of symptoms of storage diseases occurring in plants such as citrus fruits, grapes, apples, onions and the like.

According to an embodiment of the present invention, a composition for controlling plant storage bottles is provided, which contains tobacco root extract as an active ingredient.

According to one side, wherein the plant storage disease may be caused by Aspergillus (Aspergillus) in or penny silryum (Penicillium) in fungi, more specifically Aspergillus nigeo (Aspergillus niger), Penny silryum Italian glutamicum ( Penicillium italicum), Penny silryum X may be caused by the paenseom (Penicilium expansum).

In addition, the plant in which the plant storage disease develops may be an apple, pear, grape, garlic, onion, cucumber, melon, tomato or citrus.

Tobacco root extract contained in the composition of the present invention may be extracted from brazilian mappacho, black rosica or yellow tobacco. Brazil mapacho is a species grown in the highlands or cold regions of South America, mainly in Mexico, and in Germany and Switzerland. The nicotine content of leaves is higher than that of conventional tobacco, so it is grown for nicotine harvesting. Black rustica is Nicotiana A tobacco belonging to the Rustica species, used together with Nicotiana tabacum for the manufacture of tobacco tobacco. Yellow species ( Nicotiana tabacum . L , Accession No. KF109 (3506), (Deposit: National Species Resources, Registered Date 2011.04.22)) are varieties of leaf tobacco cultivated mainly in Korea.

Tobacco root extract of the present invention may be obtained by using at least one selected from acetone, alcohol, lower alcohol having 1 to 4 carbon atoms, ethyl acetate and 1,3-butylene glycol as the extraction solvent.

According to one side, the tobacco root extract may be 70% acetone extract of brazilian mapacho root, 70% acetone extract of black rosica root or ethanol extract of yellow species root. 70% acetone extracts of brazilian maple leaf and black rosica root and ethanol extracts of yellow seed root showed better antimicrobial activity than other solvent extracts. It shows better antimicrobial activity compared to the ethanol extract of the root (see Example 3, Table 2).

According to another embodiment of the present invention, there is provided a plant storage bottle control agent comprising a composition according to an embodiment of the present invention.

Control agents of the present invention may be formulated in the form of granules, liquids, hydrates, granules, dispersants or capsules mixed with an agriculturally acceptable carrier, but is not limited thereto. Liquids and hydrating agents can be diluted to 1/1000 as needed.

In the above, the "agrochemically acceptable carrier" is combined with the active ingredient of the control agent composition of the present invention to promote the spreading, storage and / or transport of the active ingredient without inhibiting the activity of the active ingredient and to plant or human body. Any substance that does not exhibit acceptable toxicity.

The control agent of the present invention may further comprise at least one auxiliary agent selected from surfactants, pressure-sensitive adhesives, extenders, wetting agents, dispersants and nutrients in the plant storage bottle control composition of the present invention. Such adjuvants may be selected and used as appropriate from those known in the art.

For example, the surfactant may be polycarboxylate, sodium lignosulfonate, calcium lignosulfonate, sodium dialkyl sulfosuccinate, sodium alkyl aryl sulfonate, polyoxyethylene alkyl phenyl ether, sodium tripolyphosphate, poly From oxyethylene alkyl aryl phosphoric esters, polyoxyethylene alkyl aryl ethers, polyoxyethylene alkyl aryl polymers, polyoxyalkylone alkyl phenyl ethers, polyoxyethylene nonyl phenyl ethers, sodium sulfonate naphthalene formaldehyde, Triton 100 and Tween 80 One or more selected may be used.

Adhesives include starch, hide glue, casein, dextrin, plant gum, animal protein, plant protein, shellac, natural rubber, sodium silicate, alkyd, acrylic ester, polyamide, polystyrene, synthetic rubber, polyvinyl alcohol, urea One or more selected from resins, phenolic resins, unsaturated polyester resins, epoxy resins, melamine resins, furan resins, and alkyd resins can be used.

Alkylnaphthalene sulfonates (particularly diisopropylnaphthalenesulfonate or diisobutylnaphthalenesulfonate) can be used as wetting agents, and nonionic dispersants (ethylene oxide / propylene oxide block polymers, alkylphenol polyglycol ethers, triglycerides) can be used as dispersants. Styrylphenol polyglycol, etc.), anionic dispersants (ligninsulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, dibutylnaphthalenesulfonic acid, alkylarylsulfonates, alkyl sulfates, alkylsulfonates, etc.) or mixtures thereof can be used.

Extenders and nutrients include sodium alginate, starch, soybean meal, bran, granular fiber, yuan, diatomaceous earth, zeolite, bentonite, talc, kaolin, pyrophyllite, white carbon, sugars, soy flour, rice, wheat, ocher and dextrin One or more selected may be used. In addition, depending on the formulation of the control agent, the purpose of use may further include an antifreeze, preservatives, electrodeposition disintegrants and the like.

According to another embodiment of the present invention, preparing a yellow species, brazilian mapocho or black rosica tobacco; Preparing an extract by immersing the tobacco in an extraction solvent; And concentrating the extract by filtration. It includes, a method for producing a composition for controlling plant storage bottles is provided.

According to one side, after the filtration and concentration step, adding at least one adjuvant selected from surfactants, tackifiers, extenders, wetting agents, dispersants and nutrients; It may further include.

As described above, the extraction solvent may be at least one selected from acetone, alcohol, lower alcohols having 1 to 4 carbon atoms, ethyl acetate, and 1,3-butylene glycol, more preferably 70% acetone or ethanol Can be.

The plant storage bottle control agent of the present invention is preferably treated by the method of foliage treatment on the plant ground part from the 10th day after planting the crop or immediately before the onset of the disease, but is not limited thereto, and is sprayed immediately before harvesting the crop. You can also use the method.

Hereinafter, the present invention will be described in more detail with reference to Examples. The following examples are described for the purpose of illustrating the present invention, but the scope of the present invention is not limited thereto.

Example  1: Preparation of Tobacco Root Extract

The roots of Black Rustica, Brazilian Mapocho and Yellow Tobacco were prepared and chopped. 100g of the cut tobacco roots were immersed in 1L 70% acetone solution and extracted for 7 days at room temperature, and hot water extraction (90 ° C., 3 hours) was immersed in the same amount of 70% ethanol solution, 100% ethanol solution and sterile distilled water. It was. Each of them was filtered and concentrated to prepare a tobacco root extract.

Example  2: Plant storage bottles  Evaluation of antimicrobial activity against induced bacteria

Aspergillus niger , Penicilium italicum , Penicilium expansum was prepared by receiving the strains stored in the Rural Development Administration. Strained Aspergillus niger strains were used in PDA medium (potato dextrose agar, Difco Co.), Penicilium italicum, Penicilium expansum strain was cultured for 5 to 7 days 25 ℃ using a MEA medium (malt extract agar, Difco CO.) by collecting the spores formed were used as inoculum. The spores formed were inoculated into a Erlenmeyer flask containing 100 ml of PDB (potato dextrose broth), MEB (malt extract broth) medium, and incubated at 120C for 4 days (120 rpm). The cultured mycelium was homogenized using a blender (Warring blander, USA), and then mixed in a 10% ratio to the upper MEA medium (0.8% agar), and 10 ml aliquots were dispensed onto the lower MEA medium to prepare the assay medium. It was.

Paper disk (diameter 8 mm) to which the 70% acetone tobacco root extract prepared in Example 1 was added was placed on the assay medium and incubated at 25 ° C. for 3 to 4 days to inhibit growth (inhibition zone). Size, mm) was measured and the results are shown in Table 1 below.

sample Growth inhibition effect by the amount of sample added (inhibition zone, mm) A. niger P. italicum P. expansum 20mg 15 mg 10mg 5mg 20mg 15 mg 10mg 5mg 20mg 15 mg 10mg 5mg Yellow bell 25 22 16.5 11.5 12 - - - 9 - - - Brazilian Mapocho 30 28 26.5 21 30 28 26 16 14 10 - - Black Rustica 33.5 31 26 22 25 20 14.5 - 12 - - -

Through the results shown in Table 1, the tobacco root extract showed an excellent growth inhibitory effect on A. niger, it can be confirmed that the growth inhibition effect is concentration dependent. In particular, the root extracts of brazilian mappacho and black rosica are found to have inhibitory effects on P. italicum and P. expanson as well as A. niger .

Example  3: Extraction solvent  Antimicrobial Activity Evaluation by Star

Aspergillus niger , Penicilium of the solvent-specific extract prepared in Example 1 using the method described in Example 2 italicum , Penicilium Growth inhibition of the expansum was measured, and the results are shown in Table 2 below.

sample Extraction solvent Growth Inhibition Effect by Extraction Solvent (inhibition zone, mm / 15mg loading) A. niger P. italicum P. expansum Yellow bell 70% acetone 22 18 - 70% ethanol 22 12 - 100% ethanol 26 14 15 Sterile distilled water 14.5 20 - Brazilian Mapocho 70% acetone 30 16 17 70% ethanol 22 12 - 100% ethanol 24 14 - Sterile distilled water 20 - - Black Rustica 70% acetone 24 18 - 70% ethanol 18 14 - 100% ethanol 22 16 - Sterile distilled water 18 12 -

Through the results shown in Table 2, it can be seen that the roots of brazilian mapacho and black rosica are 70% acetone extracts, yellow species roots, ethanol extract is superior to the growth inhibitory effect compared to extracts using other solvents. Therefore, it is preferable that the composition for controlling plant storage bottles contains Brazilian Mapocho, 70% acetone extract of black rootica root or ethanol extract of yellow species root as an active ingredient.

Example  4: Evaluation of antimicrobial activity by tobacco parts

After preparing and cutting finely the yellow leaf and stem, 100 g of the cut yellow leaf and stem was immersed in 1L 70% acetone solution and extracted for 7 days at room temperature, and then filtered and concentrated to extract tobacco leaf and stem extract. Prepared. Root extract of the yellow species was prepared in Example 1 above. Aspergillus niger , Penicilium italicum , Penicilium by the method described in Example 2 with the addition amount of 70% acetone extract of the leaves, stems and roots to 15mg Growth inhibition of the expansum was measured and shown in Table 3 below.

sample part Growth inhibition effect by extraction site (inhibition zone, mm / 15mg loading) A. niger P. italicum P. expansum Yellow bell Root 23 17 - leaf - - - stem - - -

As can be seen in Table 3 above, when using a leaf, stem extract Aspergillus niger , Penicilium italicum , Penicilium expansum It did not show antimicrobial activity against. The above results indicate that tobacco root extract is Aspergillus niger , Penicilium italicum , Penicilium This suggests that it can be usefully used to control plant storage diseases caused by expansum .

Example  5: citrus Storage bottle  Control effect test

After disinfecting the tangerines shipped from Jeju Island with 2% sodium hypochlorite (NaOCl) solution, use a needle (bundled 5) to cut the surface (? 3㎜) and use Aspergillus niger , Penicillium italicum , Penicillium expansun spore suspension was inoculated. One hour after inoculation, 50 µl (100 mg / ml) of the 70% hydrous acetone extract prepared in Example 1 was injected into the wound and plated at a radius of 2 cm. Then, the tissue was placed in a plastic box (70% ethanol sterilization) on which a tissue was soaked with sterile water and injured in a 25 ° C. thermostat for 5 days, and the degree of disease was observed.

As a result, as shown in Figure 1, brazilian mappacho, black rosica, and yellow species root extract are allAspergillus niger, Penicillium italicum , Penicillium expansunReduction by bacteria and production of mycelia or spores were suppressed. In particular, brazilian mapacho and black rosica root extracts showed superior anti-microbial inhibitory effect compared to the yellow root extract. This result, the tobacco root extract of the present inventionAspergillus niger, Penicillium italicum , Penicillium expansun This suggests that it can be usefully used to control plant storage diseases caused by bacteria.

Although the embodiments have been described with reference to the accompanying drawings, those skilled in the art may apply various technical modifications and variations based on the above. For example, the techniques described may be performed in a different order than the described method, and / or the components described may be combined or combined in a different form than the described method, or replaced or substituted by other components or equivalents. Appropriate results can be achieved.

Therefore, other implementations, other embodiments, and equivalents to the claims are within the scope of the following claims.

Claims (12)

A composition for controlling plant storage bottles, containing tobacco root extract as an active ingredient.
The method of claim 1,
The plant storage bottle is caused by the genus Aspergillus ( Aspergillus ) or Penicillium ( Penicillium) , the composition for controlling plant storage bottles.
The method of claim 2,
The Aspergillus genus Aspergillus nigeo (Aspergillus niger), and the genus of the penny silryum penny silryum Italian glutamicum (Penicillium italicum ), Penicilium expansum ( Penicilium expansum ), a composition for controlling plant storage bottles.
The method of claim 1,
The tobacco root extract is extracted from brazilian mapacho, black rosica or yellow tobacco, plant storage bottle control composition.
The method of claim 4, wherein
The tobacco root extract is obtained by acetone, alcohol, lower alcohols having 1 to 4 carbon atoms, ethyl acetate and 1,3-butylene glycol obtained as an extraction solvent, plant storage bottle control composition.
The method of claim 5,
The tobacco root extract is a 70% acetone extract of the roots of brazilian mapacho, 70% acetone extract of the black rosica root or ethanol extract of the yellow species root, plant storage bottle control composition.
A plant storage bottle control agent comprising the composition of any one of claims 1 to 6.
The method of claim 7, wherein
The control agent is a plant storage bottle control agent in the form of granules, liquids, hydrates, granules, dispersants or capsules.
The method of claim 7, wherein
The control agent further comprises at least one auxiliary agent selected from surfactants, pressure-sensitive adhesives, extenders, wetting agents, dispersants and nutrients, plant storage bottle control agent.
Preparing the roots of yellow, brazilian, or black Rustic tobacco;
Preparing an extract by immersing the root of the tobacco in an extraction solvent; And
Filtering and extracting the extract; Comprising a method for producing a composition for controlling plant storage bottles.
The method of claim 10,
After the filtration and concentration steps,
Adding at least one adjuvant selected from surfactants, tackifiers, extenders, wetting agents, dispersants and nutrients; Further comprising, a method for producing a composition for controlling plant storage bottles.
The method according to claim 10 or 11, wherein
The extraction solvent is at least one selected from acetone, alcohol, lower alcohol having 1 to 4 carbon atoms, ethyl acetate, and 1,3-butylene glycol, the method for producing a plant storage bottle control composition.

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