JPWO2022181660A5 - - Google Patents
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- JPWO2022181660A5 JPWO2022181660A5 JP2023502465A JP2023502465A JPWO2022181660A5 JP WO2022181660 A5 JPWO2022181660 A5 JP WO2022181660A5 JP 2023502465 A JP2023502465 A JP 2023502465A JP 2023502465 A JP2023502465 A JP 2023502465A JP WO2022181660 A5 JPWO2022181660 A5 JP WO2022181660A5
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- JP
- Japan
- Prior art keywords
- vldl
- cell culture
- fatty liver
- substance
- test substance
- Prior art date
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- 238000004113 cell culture Methods 0.000 claims 9
- 108010062497 VLDL Lipoproteins Proteins 0.000 claims 8
- 239000000126 substance Substances 0.000 claims 5
- 208000004930 Fatty Liver Diseases 0.000 claims 3
- 206010019708 Hepatic steatosis Diseases 0.000 claims 3
- 108010007622 LDL Lipoproteins Proteins 0.000 claims 3
- 102000007330 LDL Lipoproteins Human genes 0.000 claims 3
- 239000012228 culture supernatant Substances 0.000 claims 3
- 208000010706 fatty liver disease Diseases 0.000 claims 3
- 210000003494 hepatocyte Anatomy 0.000 claims 3
- 238000000034 method Methods 0.000 claims 3
- 231100000240 steatosis hepatitis Toxicity 0.000 claims 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims 2
- 230000000370 effect on dyslipidemia Effects 0.000 claims 2
- 238000012216 screening Methods 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 238000004264 monolayer culture Methods 0.000 claims 1
Claims (6)
- 培養上清中の超低密度リポタンパク質（ＶＬＤＬ）のうちＬａｒｇｅ－ＶＬＤＬの増減を指標にして、脂質異常に効果を有する物質のスクリーニング方法に使用するための、ヒト脂肪肝モデル細胞培養物であって、
該細胞の培養上清がＶＬＤＬ及び低密度リポタンパク質（ＬＤＬ）を含有し、該ＶＬＤＬが該ＬＤＬよりも多く含むことを特徴とする、細胞培養物。 - 単層細胞培養物である、請求項１に記載の細胞培養物。
- 前記ＶＬＤＬが、Ｌａｒｇｅ－ＶＬＤＬ、Ｍｅｄｉｕｍ－ＶＬＤＬ、及びＳｍａｌｌ－ＶＬＤＬを含み、該Ｌａｒｇｅ－ＶＬＤＬをＶＬＤＬ全体の７０質量％以上の割合で含む、請求項１又は２に記載の細胞培養物。
- 脂肪肝由来のヒト肝細胞を、ジメチルスルホキシドを含む培地中にて培養して得られた細胞培養物である、請求項１～３のいずれか一項に記載の細胞培養物。
- 脂肪肝由来のヒト肝細胞が、ヒト肝細胞を有するキメラ非ヒト動物より回収されたものである、請求項４に記載の細胞培養物。
- 脂質異常に効果を有する物質のスクリーニング方法であって、
請求項１～５のいずれか１項に記載の細胞培養物に被検物質を投与する工程、ならびに、
被験物質を投与した細胞培養物と投与されていない細胞培養物との間で培養上清中の超低密度リポタンパク質（ＶＬＤＬ）のうちＬａｒｇｅ－ＶＬＤＬの量を比較する工程、を含む方法。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021030911 | 2021-02-26 | ||
| JP2021030911 | 2021-02-26 | ||
| PCT/JP2022/007494 WO2022181660A1 (ja) | 2021-02-26 | 2022-02-24 | スクリーニング方法に使用するためのヒト脂肪肝モデル細胞 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JPWO2022181660A1 JPWO2022181660A1 (ja) | 2022-09-01 |
| JPWO2022181660A5 true JPWO2022181660A5 (ja) | 2023-07-13 |
| JP7449626B2 JP7449626B2 (ja) | 2024-03-14 |
Family
ID=83049072
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023502465A Active JP7449626B2 (ja) | 2021-02-26 | 2022-02-24 | スクリーニング方法に使用するためのヒト脂肪肝モデル細胞 |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP4299720A1 (ja) |
| JP (1) | JP7449626B2 (ja) |
| CN (1) | CN116917462A (ja) |
| CA (1) | CA3211975A1 (ja) |
| WO (1) | WO2022181660A1 (ja) |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2146735C (en) | 1994-04-11 | 2007-02-20 | Chise Tateno | Liver parenchymal cells having clonal growth ability, method for obtaining same, method for subculturing same, and subculturing system of primary hepatocytes |
| JP3157984B2 (ja) | 1994-04-11 | 2001-04-23 | 科学技術振興事業団 | 初代培養肝細胞の継代培養系 |
| JP3266766B2 (ja) | 1994-08-23 | 2002-03-18 | 科学技術振興事業団 | ローン性増殖能を有する肝実質細胞とその取得方法、並びにその継代培養方法 |
| JPH0915225A (ja) | 1995-04-28 | 1997-01-17 | Tosoh Corp | 血清リポタンパク質の分析方法 |
| JP3014322B2 (ja) | 1996-05-28 | 2000-02-28 | 科学技術振興事業団 | 小型肝細胞の培養方法 |
| JP3211941B2 (ja) | 1996-12-26 | 2001-09-25 | 科学技術振興事業団 | ヒト小型肝細胞の取得方法と、この細胞の初代培養および継代培養方法 |
| JP2002045087A (ja) | 2000-05-19 | 2002-02-12 | Japan Science & Technology Corp | キメラ動物 |
| WO2007052789A1 (ja) | 2005-11-04 | 2007-05-10 | Skylight Biotech Inc. | 大血管障害を判別するためのリポタンパク質の分析方法 |
| JP2007228962A (ja) | 2006-01-31 | 2007-09-13 | Hiroshima Industrial Promotion Organization | ヒト肝細胞キメララットの作製方法 |
| US8278499B2 (en) | 2006-06-29 | 2012-10-02 | Hiroshima Industrial Promotion Organization | Nonalcoholic steatohepatitis model rodent and fatty liver model rodent |
| CA2868292A1 (en) | 2012-03-27 | 2013-10-03 | National University Corporation Kumamoto University | Immunodeficient mouse with humanized liver |
| JP2014204713A (ja) * | 2013-03-21 | 2014-10-30 | 秋田県 | 肝細胞分化ステージを評価する新規測定法 |
| JP7379934B2 (ja) | 2019-08-26 | 2023-11-15 | マツダ株式会社 | 車両の後部構造 |
| JP7039045B2 (ja) * | 2019-08-26 | 2022-03-22 | 株式会社フェニックスバイオ | ヒト脂肪肝モデル細胞 |
-
2022
- 2022-02-24 JP JP2023502465A patent/JP7449626B2/ja active Active
- 2022-02-24 EP EP22759695.4A patent/EP4299720A1/en active Pending
- 2022-02-24 CN CN202280017368.1A patent/CN116917462A/zh active Pending
- 2022-02-24 CA CA3211975A patent/CA3211975A1/en active Pending
- 2022-02-24 WO PCT/JP2022/007494 patent/WO2022181660A1/ja active Application Filing
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