JPWO2022098993A5 - - Google Patents
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- JPWO2022098993A5 JPWO2022098993A5 JP2023527427A JP2023527427A JPWO2022098993A5 JP WO2022098993 A5 JPWO2022098993 A5 JP WO2022098993A5 JP 2023527427 A JP2023527427 A JP 2023527427A JP 2023527427 A JP2023527427 A JP 2023527427A JP WO2022098993 A5 JPWO2022098993 A5 JP WO2022098993A5
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- JP
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- crispr
- protein
- nucleic acid
- seq
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- 238000000034 method Methods 0.000 claims description 159
- 239000012636 effector Substances 0.000 claims description 152
- 102000004169 proteins and genes Human genes 0.000 claims description 134
- 108090000623 proteins and genes Proteins 0.000 claims description 134
- 150000007523 nucleic acids Chemical class 0.000 claims description 124
- 102000039446 nucleic acids Human genes 0.000 claims description 105
- 108020004707 nucleic acids Proteins 0.000 claims description 105
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- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 76
- 108020001507 fusion proteins Proteins 0.000 claims description 63
- 102000037865 fusion proteins Human genes 0.000 claims description 63
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 53
- 229920001184 polypeptide Polymers 0.000 claims description 52
- 230000027455 binding Effects 0.000 claims description 32
- 108091033409 CRISPR Proteins 0.000 claims description 29
- 239000002773 nucleotide Substances 0.000 claims description 29
- 125000003729 nucleotide group Chemical group 0.000 claims description 29
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 22
- 108020004414 DNA Proteins 0.000 claims description 18
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- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 18
- 108091008324 binding proteins Proteins 0.000 claims description 17
- 230000006780 non-homologous end joining Effects 0.000 claims description 17
- 101150022010 gam gene Proteins 0.000 claims description 11
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 10
- 108010077544 Chromatin Proteins 0.000 claims description 8
- 210000003483 chromatin Anatomy 0.000 claims description 8
- -1 H1G Proteins 0.000 claims description 5
- 230000004570 RNA-binding Effects 0.000 claims description 5
- 230000003993 interaction Effects 0.000 claims description 5
- 238000010354 CRISPR gene editing Methods 0.000 claims description 4
- 108091028113 Trans-activating crRNA Proteins 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 102100031235 Chromodomain-helicase-DNA-binding protein 1 Human genes 0.000 claims description 3
- 102000052510 DNA-Binding Proteins Human genes 0.000 claims description 3
- 101710116602 DNA-Binding protein G5P Proteins 0.000 claims description 3
- 101100391812 Escherichia phage Mu gam gene Proteins 0.000 claims description 3
- 101000777047 Homo sapiens Chromodomain-helicase-DNA-binding protein 1 Proteins 0.000 claims description 3
- 101710162453 Replication factor A Proteins 0.000 claims description 3
- 101710176758 Replication protein A 70 kDa DNA-binding subunit Proteins 0.000 claims description 3
- 101710176276 SSB protein Proteins 0.000 claims description 3
- 101710126859 Single-stranded DNA-binding protein Proteins 0.000 claims description 3
- 108020004459 Small interfering RNA Proteins 0.000 claims description 3
- 239000013000 chemical inhibitor Substances 0.000 claims description 3
- 238000003197 gene knockdown Methods 0.000 claims description 3
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- 230000004850 protein–protein interaction Effects 0.000 claims description 2
- 102100034343 Integrase Human genes 0.000 claims 13
- 102000023732 binding proteins Human genes 0.000 claims 5
- 230000011559 double-strand break repair via nonhomologous end joining Effects 0.000 claims 5
- 235000018102 proteins Nutrition 0.000 description 120
- 102100031780 Endonuclease Human genes 0.000 description 63
- 108060002716 Exonuclease Proteins 0.000 description 32
- 102000013165 exonuclease Human genes 0.000 description 32
- 108090000652 Flap endonucleases Proteins 0.000 description 23
- 102000004150 Flap endonucleases Human genes 0.000 description 23
- 235000001014 amino acid Nutrition 0.000 description 21
- 102000040430 polynucleotide Human genes 0.000 description 20
- 108091033319 polynucleotide Proteins 0.000 description 20
- 239000002157 polynucleotide Substances 0.000 description 20
- 108020001580 protein domains Proteins 0.000 description 19
- 230000014789 establishment of RNA localization Effects 0.000 description 15
- 101150088049 dna2 gene Proteins 0.000 description 13
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- 230000035772 mutation Effects 0.000 description 10
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- 101710163270 Nuclease Proteins 0.000 description 7
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- 108700004991 Cas12a Proteins 0.000 description 5
- 108020004705 Codon Proteins 0.000 description 5
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- 230000004048 modification Effects 0.000 description 5
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- 108010008532 Deoxyribonuclease I Proteins 0.000 description 4
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 4
- 101000918264 Homo sapiens Exonuclease 1 Proteins 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 102000045455 human EXO1 Human genes 0.000 description 4
- 238000005457 optimization Methods 0.000 description 4
- 125000006850 spacer group Chemical group 0.000 description 4
- 101710132601 Capsid protein Proteins 0.000 description 3
- 101710094648 Coat protein Proteins 0.000 description 3
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 101710141454 Nucleoprotein Proteins 0.000 description 3
- 101710083689 Probable capsid protein Proteins 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 108010011170 Ala-Trp-Arg-His-Pro-Gln-Phe-Gly-Gly Proteins 0.000 description 2
- 241000203069 Archaea Species 0.000 description 2
- 101710185850 Exodeoxyribonuclease Proteins 0.000 description 2
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- 108020005004 Guide RNA Proteins 0.000 description 2
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
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- 239000003446 ligand Substances 0.000 description 2
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- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 241000604451 Acidaminococcus Species 0.000 description 1
- 101710159080 Aconitate hydratase A Proteins 0.000 description 1
- 101710159078 Aconitate hydratase B Proteins 0.000 description 1
- 108091023037 Aptamer Proteins 0.000 description 1
- 101100107610 Arabidopsis thaliana ABCF4 gene Proteins 0.000 description 1
- 241000605902 Butyrivibrio Species 0.000 description 1
- 230000007018 DNA scission Effects 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
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- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 102000015335 Ku Autoantigen Human genes 0.000 description 1
- 108010025026 Ku Autoantigen Proteins 0.000 description 1
- 241001112693 Lachnospiraceae Species 0.000 description 1
- 101710135898 Myc proto-oncogene protein Proteins 0.000 description 1
- 102100038895 Myc proto-oncogene protein Human genes 0.000 description 1
- 102000009572 RNA Polymerase II Human genes 0.000 description 1
- 108010009460 RNA Polymerase II Proteins 0.000 description 1
- 108091008103 RNA aptamers Proteins 0.000 description 1
- 102000044126 RNA-Binding Proteins Human genes 0.000 description 1
- 101710105008 RNA-binding protein Proteins 0.000 description 1
- 101100068078 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GCN4 gene Proteins 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 101710150448 Transcriptional regulator Myc Proteins 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
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- 210000004671 cell-free system Anatomy 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000006471 dimerization reaction Methods 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 125000001475 halogen functional group Chemical group 0.000 description 1
- 239000000833 heterodimer Substances 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- ZAHRKKWIAAJSAO-UHFFFAOYSA-N rapamycin Natural products COCC(O)C(=C/C(C)C(=O)CC(OC(=O)C1CCCCN1C(=O)C(=O)C2(O)OC(CC(OC)C(=CC=CC=CC(C)CC(C)C(=O)C)C)CCC2C)C(C)CC3CCC(O)C(C3)OC)C ZAHRKKWIAAJSAO-UHFFFAOYSA-N 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- QFJCIRLUMZQUOT-HPLJOQBZSA-N sirolimus Chemical compound C1C[C@@H](O)[C@H](OC)C[C@@H]1C[C@@H](C)[C@H]1OC(=O)[C@@H]2CCCCN2C(=O)C(=O)[C@](O)(O2)[C@H](C)CC[C@H]2C[C@H](OC)/C(C)=C/C=C/C=C/[C@@H](C)C[C@@H](C)C(=O)[C@H](OC)[C@H](O)/C(C)=C/[C@@H](C)C(=O)C1 QFJCIRLUMZQUOT-HPLJOQBZSA-N 0.000 description 1
- 229960002930 sirolimus Drugs 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063110386P | 2020-11-06 | 2020-11-06 | |
| US63/110,386 | 2020-11-06 | ||
| PCT/US2021/058235 WO2022098993A2 (en) | 2020-11-06 | 2021-11-05 | Compositions and methods for rna-encoded dna-replacement of alleles |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2023549339A JP2023549339A (ja) | 2023-11-24 |
| JP2023549339A5 JP2023549339A5 (https=) | 2024-11-13 |
| JPWO2022098993A5 true JPWO2022098993A5 (https=) | 2024-11-13 |
Family
ID=79927158
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023527427A Pending JP2023549339A (ja) | 2020-11-06 | 2021-11-05 | 対立遺伝子の、ｒｎａによりコードされるｄｎａ置換のための組成物および方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20220145334A1 (https=) |
| EP (1) | EP4240844A2 (https=) |
| JP (1) | JP2023549339A (https=) |
| KR (1) | KR20230106633A (https=) |
| CN (1) | CN116745418A (https=) |
| AU (1) | AU2021374974A1 (https=) |
| CA (1) | CA3200521A1 (https=) |
| CL (1) | CL2023001284A1 (https=) |
| IL (1) | IL302526A (https=) |
| MX (1) | MX2023005150A (https=) |
| WO (1) | WO2022098993A2 (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115279898A (zh) | 2019-10-23 | 2022-11-01 | 成对植物服务股份有限公司 | 用于植物中rna模板化编辑的组合物和方法 |
| CA3160186A1 (en) | 2019-11-05 | 2021-05-14 | Pairwise Plants Services, Inc. | Compositions and methods for rna-encoded dna-replacement of alleles |
| WO2022265905A2 (en) | 2021-06-14 | 2022-12-22 | Pairwise Plants Services, Inc. | Reporter constructs, compositions comprising the same, and methods of use thereof |
| US20230266293A1 (en) | 2021-09-21 | 2023-08-24 | Pairwise Plants Services, Inc. | Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same |
| EP4448742A1 (en) * | 2021-12-17 | 2024-10-23 | Massachusetts Institute Of Technology | Programmable insertion approaches via reverse transcriptase recruitment |
| EP4634375A2 (en) | 2022-12-16 | 2025-10-22 | Pairwise Plants Services, Inc. | Fusion proteins comprising a cas12a polypeptide and an intein and methods of use therof |
| AU2023413056A1 (en) | 2022-12-21 | 2025-06-12 | Pairwise Plants Services, Inc. | Engineered proteins and methods of use thereof |
| IL326806A (en) * | 2023-08-31 | 2026-04-01 | Basf Agricultural Solutions Us Llc | CRISPR-CAS type V primer editing, class 2 |
| WO2025072453A1 (en) | 2023-09-26 | 2025-04-03 | Pairwise Plants Services, Inc. | Engineered deaminases, compositions comprising the same, and methods of use thereof |
| US20250129348A1 (en) | 2023-10-09 | 2025-04-24 | Pairwise Plants Services, Inc. | Circular permutants, compositions comprising the same, and methods of use thereof |
| US20250207154A1 (en) * | 2023-12-21 | 2025-06-26 | Pairwise Plants Services, Inc. | Compositions and methods for rna-encoded dna-replacement of alleles |
| WO2026019871A1 (en) | 2024-07-17 | 2026-01-22 | Pairwise Plants Services, Inc. | Engineered cytidine deaminases |
| US20260022361A1 (en) | 2024-07-17 | 2026-01-22 | Pairwise Plants Services, Inc. | Engineered proteins and methods of use thereof |
| CN120366358B (zh) * | 2025-06-25 | 2025-10-10 | 中国农业大学 | 一种毕赤酵母高效基因编辑系统、其构建方法及在高产虫草素工程菌株中的应用 |
Family Cites Families (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ221259A (en) | 1986-07-31 | 1990-05-28 | Calgene Inc | Seed specific transcriptional regulation |
| US6040504A (en) | 1987-11-18 | 2000-03-21 | Novartis Finance Corporation | Cotton promoter |
| ES2060765T3 (es) | 1988-05-17 | 1994-12-01 | Lubrizol Genetics Inc | Sistema promotor de ubiquitina en plantas. |
| US5641876A (en) | 1990-01-05 | 1997-06-24 | Cornell Research Foundation, Inc. | Rice actin gene and promoter |
| EP0452269B1 (en) | 1990-04-12 | 2002-10-09 | Syngenta Participations AG | Tissue-preferential promoters |
| US5459252A (en) | 1991-01-31 | 1995-10-17 | North Carolina State University | Root specific gene promoter |
| CA2116449C (en) | 1991-08-27 | 2005-04-05 | Vaughan Alan Hilder | Proteins with insecticidal properties against homopteran insects and their use in plant protection |
| UA48104C2 (uk) | 1991-10-04 | 2002-08-15 | Новартіс Аг | Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника |
| CA2319079A1 (en) | 1998-02-20 | 1999-08-26 | Zeneca Limited | Pollen specific promoter |
| AR044724A1 (es) | 2000-03-27 | 2005-10-05 | Syngenta Participations Ag | Promotores del virus de la rizadura amarilla del cestrum |
| IL151886A (en) | 2000-03-27 | 2010-11-30 | Syngenta Participations Ag | Promoters of a virus that causes curling of a yellow leaf of a cyst |
| ATE495264T1 (de) | 2003-10-06 | 2011-01-15 | Syngenta Participations Ag | In pflanzenplastiden funktionsfähiger promotor |
| KR100537955B1 (ko) | 2003-10-29 | 2005-12-20 | 학교법인고려중앙학원 | 꽃가루 특이적 유전자 발현 프로모터 |
| AU2013205606B2 (en) | 2012-02-01 | 2015-05-21 | Corteva Agriscience Llc | Synthetic Brassica-derived chloroplast transit peptides |
| WO2016020856A2 (en) | 2014-08-05 | 2016-02-11 | MabQuest SA | Immunological reagents |
| US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| WO2018022634A1 (en) * | 2016-07-26 | 2018-02-01 | The General Hospital Corporation | Variants of crispr from prevotella and francisella 1 (cpf1) |
| KR102523217B1 (ko) * | 2017-07-11 | 2023-04-20 | 시그마-알드리치 컴퍼니., 엘엘씨 | 표적된 게놈 변형을 개선하기 위한 뉴클레오솜 상호작용 단백질 도메인 사용 |
| US11268092B2 (en) * | 2018-01-12 | 2022-03-08 | GenEdit, Inc. | Structure-engineered guide RNA |
| CN111902536B (zh) * | 2018-02-15 | 2024-07-30 | 西格马-奥尔德里奇有限责任公司 | 用于真核基因组修饰的改造的cas9系统 |
| AU2019266327B2 (en) * | 2018-05-11 | 2025-11-06 | Beam Therapeutics Inc. | Methods of editing single nucleotide polymorphism using programmable base editor systems |
| JP7558929B2 (ja) * | 2018-05-11 | 2024-10-01 | ビーム セラピューティクス インク． | プログラム可能塩基エディターシステムを用いて病原性変異を抑制する方法 |
| WO2020018166A1 (en) * | 2018-07-16 | 2020-01-23 | The Regents Of The University Of Michigan | Nuclease-mediated nucleic acid modification |
| JP7657726B2 (ja) * | 2019-03-19 | 2025-04-07 | ザ ブロード インスティテュート，インコーポレーテッド | 編集ヌクレオチド配列を編集するための方法および組成物 |
| CA3160186A1 (en) * | 2019-11-05 | 2021-05-14 | Pairwise Plants Services, Inc. | Compositions and methods for rna-encoded dna-replacement of alleles |
| EP4085141A4 (en) * | 2019-12-30 | 2024-03-06 | The Broad Institute, Inc. | GENOME EDITING USING REVERSE TRANSCRIPTASE FOR FULLY ACTIVE CRISPR COMPLEXES |
| EP4217490A2 (en) * | 2020-09-24 | 2023-08-02 | The Broad Institute Inc. | Prime editing guide rnas, compositions thereof, and methods of using the same |
| WO2022065689A1 (ko) * | 2020-09-24 | 2022-03-31 | 고려대학교 산학협력단 | 편집 효율이 향상된 프라임 편집 기반 유전자 교정용 조성물 및 이의 용도 |
| IL308806A (en) * | 2021-06-01 | 2024-01-01 | Arbor Biotechnologies Inc | Gene editing systems including nuclease crisper and their uses |
-
2021
- 2021-11-05 JP JP2023527427A patent/JP2023549339A/ja active Pending
- 2021-11-05 MX MX2023005150A patent/MX2023005150A/es unknown
- 2021-11-05 IL IL302526A patent/IL302526A/en unknown
- 2021-11-05 US US17/520,246 patent/US20220145334A1/en active Pending
- 2021-11-05 WO PCT/US2021/058235 patent/WO2022098993A2/en not_active Ceased
- 2021-11-05 EP EP21844079.0A patent/EP4240844A2/en active Pending
- 2021-11-05 AU AU2021374974A patent/AU2021374974A1/en active Pending
- 2021-11-05 CA CA3200521A patent/CA3200521A1/en active Pending
- 2021-11-05 KR KR1020237017966A patent/KR20230106633A/ko active Pending
- 2021-11-05 CN CN202180088340.2A patent/CN116745418A/zh active Pending
-
2023
- 2023-05-03 CL CL2023001284A patent/CL2023001284A1/es unknown
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