JP2023549339A - 対立遺伝子の、ｒｎａによりコードされるｄｎａ置換のための組成物および方法 - Google Patents

対立遺伝子の、ｒｎａによりコードされるｄｎａ置換のための組成物および方法 Download PDF

Info

Publication number
JP2023549339A
JP2023549339A JP2023527427A JP2023527427A JP2023549339A JP 2023549339 A JP2023549339 A JP 2023549339A JP 2023527427 A JP2023527427 A JP 2023527427A JP 2023527427 A JP2023527427 A JP 2023527427A JP 2023549339 A JP2023549339 A JP 2023549339A
Authority
JP
Japan
Prior art keywords
crispr
type
nucleic acid
cas effector
effector protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2023527427A
Other languages
English (en)
Japanese (ja)
Other versions
JP2023549339A5 (https=
JPWO2022098993A5 (https=
Inventor
キム，ヨンジュ
ワッツ，ジョセフ・マシュー
ヒュンメル，アーロン
ローウィット，シャイ・ジョシュア
シュヴァルク，デイヴィッド
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pairwise Plants Services Inc
Original Assignee
Pairwise Plants Services Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pairwise Plants Services Inc filed Critical Pairwise Plants Services Inc
Publication of JP2023549339A publication Critical patent/JP2023549339A/ja
Publication of JP2023549339A5 publication Critical patent/JP2023549339A5/ja
Publication of JPWO2022098993A5 publication Critical patent/JPWO2022098993A5/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2023527427A 2020-11-06 2021-11-05 対立遺伝子の、ｒｎａによりコードされるｄｎａ置換のための組成物および方法 Pending JP2023549339A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063110386P 2020-11-06 2020-11-06
US63/110,386 2020-11-06
PCT/US2021/058235 WO2022098993A2 (en) 2020-11-06 2021-11-05 Compositions and methods for rna-encoded dna-replacement of alleles

Publications (3)

Publication Number Publication Date
JP2023549339A true JP2023549339A (ja) 2023-11-24
JP2023549339A5 JP2023549339A5 (https=) 2024-11-13
JPWO2022098993A5 JPWO2022098993A5 (https=) 2024-11-13

Family

ID=79927158

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2023527427A Pending JP2023549339A (ja) 2020-11-06 2021-11-05 対立遺伝子の、ｒｎａによりコードされるｄｎａ置換のための組成物および方法

Country Status (11)

Country Link
US (1) US20220145334A1 (https=)
EP (1) EP4240844A2 (https=)
JP (1) JP2023549339A (https=)
KR (1) KR20230106633A (https=)
CN (1) CN116745418A (https=)
AU (1) AU2021374974A1 (https=)
CA (1) CA3200521A1 (https=)
CL (1) CL2023001284A1 (https=)
IL (1) IL302526A (https=)
MX (1) MX2023005150A (https=)
WO (1) WO2022098993A2 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115279898A (zh) 2019-10-23 2022-11-01 成对植物服务股份有限公司 用于植物中rna模板化编辑的组合物和方法
CA3160186A1 (en) 2019-11-05 2021-05-14 Pairwise Plants Services, Inc. Compositions and methods for rna-encoded dna-replacement of alleles
WO2022265905A2 (en) 2021-06-14 2022-12-22 Pairwise Plants Services, Inc. Reporter constructs, compositions comprising the same, and methods of use thereof
US20230266293A1 (en) 2021-09-21 2023-08-24 Pairwise Plants Services, Inc. Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same
EP4448742A1 (en) * 2021-12-17 2024-10-23 Massachusetts Institute Of Technology Programmable insertion approaches via reverse transcriptase recruitment
EP4634375A2 (en) 2022-12-16 2025-10-22 Pairwise Plants Services, Inc. Fusion proteins comprising a cas12a polypeptide and an intein and methods of use therof
AU2023413056A1 (en) 2022-12-21 2025-06-12 Pairwise Plants Services, Inc. Engineered proteins and methods of use thereof
IL326806A (en) * 2023-08-31 2026-04-01 Basf Agricultural Solutions Us Llc CRISPR-CAS type V primer editing, class 2
WO2025072453A1 (en) 2023-09-26 2025-04-03 Pairwise Plants Services, Inc. Engineered deaminases, compositions comprising the same, and methods of use thereof
US20250129348A1 (en) 2023-10-09 2025-04-24 Pairwise Plants Services, Inc. Circular permutants, compositions comprising the same, and methods of use thereof
US20250207154A1 (en) * 2023-12-21 2025-06-26 Pairwise Plants Services, Inc. Compositions and methods for rna-encoded dna-replacement of alleles
WO2026019871A1 (en) 2024-07-17 2026-01-22 Pairwise Plants Services, Inc. Engineered cytidine deaminases
US20260022361A1 (en) 2024-07-17 2026-01-22 Pairwise Plants Services, Inc. Engineered proteins and methods of use thereof
CN120366358B (zh) * 2025-06-25 2025-10-10 中国农业大学 一种毕赤酵母高效基因编辑系统、其构建方法及在高产虫草素工程菌株中的应用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019161290A1 (en) * 2018-02-15 2019-08-22 Sigma-Aldrich Co. Llc Engineered cas9 systems for eukaryotic genome modification
WO2019217941A1 (en) * 2018-05-11 2019-11-14 Beam Therapeutics Inc. Methods of suppressing pathogenic mutations using programmable base editor systems
WO2019217943A1 (en) * 2018-05-11 2019-11-14 Beam Therapeutics Inc. Methods of editing single nucleotide polymorphism using programmable base editor systems
WO2020018166A1 (en) * 2018-07-16 2020-01-23 The Regents Of The University Of Michigan Nuclease-mediated nucleic acid modification
WO2020191153A2 (en) * 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
JP2020530287A (ja) * 2017-07-11 2020-10-22 シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニーＳｉｇｍａ−Ａｌｄｒｉｃｈ Ｃｏ． ＬＬＣ 標的化されたゲノム修飾を増強するためのヌクレオソーム相互作用タンパク質ドメインの使用

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ221259A (en) 1986-07-31 1990-05-28 Calgene Inc Seed specific transcriptional regulation
US6040504A (en) 1987-11-18 2000-03-21 Novartis Finance Corporation Cotton promoter
ES2060765T3 (es) 1988-05-17 1994-12-01 Lubrizol Genetics Inc Sistema promotor de ubiquitina en plantas.
US5641876A (en) 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
EP0452269B1 (en) 1990-04-12 2002-10-09 Syngenta Participations AG Tissue-preferential promoters
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
CA2116449C (en) 1991-08-27 2005-04-05 Vaughan Alan Hilder Proteins with insecticidal properties against homopteran insects and their use in plant protection
UA48104C2 (uk) 1991-10-04 2002-08-15 Новартіс Аг Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника
CA2319079A1 (en) 1998-02-20 1999-08-26 Zeneca Limited Pollen specific promoter
AR044724A1 (es) 2000-03-27 2005-10-05 Syngenta Participations Ag Promotores del virus de la rizadura amarilla del cestrum
IL151886A (en) 2000-03-27 2010-11-30 Syngenta Participations Ag Promoters of a virus that causes curling of a yellow leaf of a cyst
ATE495264T1 (de) 2003-10-06 2011-01-15 Syngenta Participations Ag In pflanzenplastiden funktionsfähiger promotor
KR100537955B1 (ko) 2003-10-29 2005-12-20 학교법인고려중앙학원 꽃가루 특이적 유전자 발현 프로모터
AU2013205606B2 (en) 2012-02-01 2015-05-21 Corteva Agriscience Llc Synthetic Brassica-derived chloroplast transit peptides
WO2016020856A2 (en) 2014-08-05 2016-02-11 MabQuest SA Immunological reagents
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
WO2018022634A1 (en) * 2016-07-26 2018-02-01 The General Hospital Corporation Variants of crispr from prevotella and francisella 1 (cpf1)
US11268092B2 (en) * 2018-01-12 2022-03-08 GenEdit, Inc. Structure-engineered guide RNA
CA3160186A1 (en) * 2019-11-05 2021-05-14 Pairwise Plants Services, Inc. Compositions and methods for rna-encoded dna-replacement of alleles
EP4085141A4 (en) * 2019-12-30 2024-03-06 The Broad Institute, Inc. GENOME EDITING USING REVERSE TRANSCRIPTASE FOR FULLY ACTIVE CRISPR COMPLEXES
EP4217490A2 (en) * 2020-09-24 2023-08-02 The Broad Institute Inc. Prime editing guide rnas, compositions thereof, and methods of using the same
WO2022065689A1 (ko) * 2020-09-24 2022-03-31 고려대학교 산학협력단 편집 효율이 향상된 프라임 편집 기반 유전자 교정용 조성물 및 이의 용도
IL308806A (en) * 2021-06-01 2024-01-01 Arbor Biotechnologies Inc Gene editing systems including nuclease crisper and their uses

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020530287A (ja) * 2017-07-11 2020-10-22 シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニーＳｉｇｍａ−Ａｌｄｒｉｃｈ Ｃｏ． ＬＬＣ 標的化されたゲノム修飾を増強するためのヌクレオソーム相互作用タンパク質ドメインの使用
WO2019161290A1 (en) * 2018-02-15 2019-08-22 Sigma-Aldrich Co. Llc Engineered cas9 systems for eukaryotic genome modification
WO2019217941A1 (en) * 2018-05-11 2019-11-14 Beam Therapeutics Inc. Methods of suppressing pathogenic mutations using programmable base editor systems
WO2019217943A1 (en) * 2018-05-11 2019-11-14 Beam Therapeutics Inc. Methods of editing single nucleotide polymorphism using programmable base editor systems
WO2020018166A1 (en) * 2018-07-16 2020-01-23 The Regents Of The University Of Michigan Nuclease-mediated nucleic acid modification
WO2020191153A2 (en) * 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
WO2020191234A1 (en) * 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ANZALONE, A.V., ET AL.: ""Reprogramming eukaryotic translation with ligand responsive synthetic RNA switches."", NATURE METHODS, vol. 13, no. 5, JPN7025004512, May 2016 (2016-05-01), pages 453 - 458, ISSN: 0005709759 *
ANZALONE, A.V., ET AL.: ""Search-and replase genome editing without double-strand breaks or donor DNA."", NATURE, vol. 576, JPN6025005927, 5 December 2019 (2019-12-05), pages 149 - 157, ISSN: 0005709758 *
CUI, L., ET AL.: ""Consequences of Cas9 cleavage in the chromosome of Escherichia coli."", NUCLEIC ACIDS RESEARCH, vol. 44, no. 9, JPN7025004511, 19 May 2016 (2016-05-19), pages 4243 - 4251, ISSN: 0005709761 *
WANG, Y., ET AL.: ""A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in", NUCLEIC ACIDS RESEARCH, vol. 32, no. 3, JPN7025000603, 18 February 2004 (2004-02-18), pages 1197 - 1207, ISSN: 0005709760 *
YU, C.H., ET AL.: ""Exploiting preQ1 riboswitches to regulate ribosomal frameshifting."", ACS CHEMICAL BIOLOGY, vol. 8, no. 4, JPN6025042248, 17 January 2013 (2013-01-17), pages 733 - 740, ISSN: 0005822433 *

Also Published As

Publication number Publication date
US20220145334A1 (en) 2022-05-12
CN116745418A (zh) 2023-09-12
AU2021374974A9 (en) 2025-03-20
KR20230106633A (ko) 2023-07-13
WO2022098993A3 (en) 2022-06-16
IL302526A (en) 2023-07-01
MX2023005150A (es) 2023-05-26
CL2023001284A1 (es) 2023-12-22
AU2021374974A1 (en) 2023-05-25
EP4240844A2 (en) 2023-09-13
WO2022098993A2 (en) 2022-05-12
CA3200521A1 (en) 2022-05-12

Similar Documents

Publication Publication Date Title
JP7785002B2 (ja) 対立遺伝子のｒｎａにコードされたｄｎａの置換のための組成物および方法
JP2023549339A (ja) 対立遺伝子の、ｒｎａによりコードされるｄｎａ置換のための組成物および方法
CN114867852A (zh) V型crispr-cas碱基编辑器及其使用方法
AU2020369599A1 (en) Compositions and methods for RNA-templated editing in plants
JP2023539668A (ja) 操作されたＣＲＩＳＰＲ－Ｃａｓタンパク質およびその使用方法
JP7765390B2 (ja) 鋳型を用いた編集のためのｄｎａポリメラーゼのリクルート
WO2024182746A1 (en) Engineered proteins and methods of use thereof
US20240279659A1 (en) Recruitment methods and compounds, compositions and systems for recruitment
EP4634375A2 (en) Fusion proteins comprising a cas12a polypeptide and an intein and methods of use therof
US20250109389A1 (en) Fusion proteins, compositions comprising the same, and methods of use thereof
US20250207154A1 (en) Compositions and methods for rna-encoded dna-replacement of alleles
US20250129348A1 (en) Circular permutants, compositions comprising the same, and methods of use thereof
WO2025259524A1 (en) Nucleic acids for guide expression

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20241105

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20241105

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20251016

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20260116

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20260324