JPWO2021178432A5 - - Google Patents
Download PDFInfo
- Publication number
- JPWO2021178432A5 JPWO2021178432A5 JP2022552549A JP2022552549A JPWO2021178432A5 JP WO2021178432 A5 JPWO2021178432 A5 JP WO2021178432A5 JP 2022552549 A JP2022552549 A JP 2022552549A JP 2022552549 A JP2022552549 A JP 2022552549A JP WO2021178432 A5 JPWO2021178432 A5 JP WO2021178432A5
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- protein
- composition
- aptamer
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 30
- 150000007523 nucleic acids Chemical class 0.000 claims description 28
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims description 24
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims description 24
- 108091023037 Aptamer Proteins 0.000 claims description 22
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 22
- 230000000813 microbial effect Effects 0.000 claims description 22
- 239000013598 vector Substances 0.000 claims description 20
- 210000004027 cell Anatomy 0.000 claims description 18
- 108090000623 proteins and genes Proteins 0.000 claims description 18
- 108020004707 nucleic acids Proteins 0.000 claims description 16
- 102000039446 nucleic acids Human genes 0.000 claims description 16
- 102000004169 proteins and genes Human genes 0.000 claims description 16
- 108091033409 CRISPR Proteins 0.000 claims description 14
- 102000023732 binding proteins Human genes 0.000 claims description 14
- 108091008324 binding proteins Proteins 0.000 claims description 14
- 108010079855 Peptide Aptamers Proteins 0.000 claims description 12
- 108091008103 RNA aptamers Proteins 0.000 claims description 12
- 102000037865 fusion proteins Human genes 0.000 claims description 12
- 108020001507 fusion proteins Proteins 0.000 claims description 12
- 108091033319 polynucleotide Proteins 0.000 claims description 12
- 102000040430 polynucleotide Human genes 0.000 claims description 12
- 239000002157 polynucleotide Substances 0.000 claims description 12
- 101000911710 Escherichia phage T7 Exonuclease Proteins 0.000 claims description 6
- 108060002716 Exonuclease Proteins 0.000 claims description 6
- 108020005004 Guide RNA Proteins 0.000 claims description 6
- 230000000295 complement effect Effects 0.000 claims description 6
- 102000013165 exonuclease Human genes 0.000 claims description 6
- 230000030648 nucleus localization Effects 0.000 claims description 6
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims description 4
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims description 4
- 241000193996 Streptococcus pyogenes Species 0.000 claims description 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 4
- 101100107610 Arabidopsis thaliana ABCF4 gene Proteins 0.000 claims description 2
- 101710132601 Capsid protein Proteins 0.000 claims description 2
- 108700004991 Cas12a Proteins 0.000 claims description 2
- 101710094648 Coat protein Proteins 0.000 claims description 2
- 102220605874 Cytosolic arginine sensor for mTORC1 subunit 2_D10A_mutation Human genes 0.000 claims description 2
- 241000588724 Escherichia coli Species 0.000 claims description 2
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 claims description 2
- 101710125418 Major capsid protein Proteins 0.000 claims description 2
- 101710141454 Nucleoprotein Proteins 0.000 claims description 2
- 101710083689 Probable capsid protein Proteins 0.000 claims description 2
- 101100068078 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GCN4 gene Proteins 0.000 claims description 2
- 101100166144 Staphylococcus aureus cas9 gene Proteins 0.000 claims description 2
- 210000003527 eukaryotic cell Anatomy 0.000 claims description 2
- 210000005260 human cell Anatomy 0.000 claims description 2
- 238000001727 in vivo Methods 0.000 claims description 2
- 210000004962 mammalian cell Anatomy 0.000 claims description 2
- 238000012986 modification Methods 0.000 claims description 2
- 230000004048 modification Effects 0.000 claims description 2
- 230000007115 recruitment Effects 0.000 claims description 2
- 210000000130 stem cell Anatomy 0.000 claims description 2
- 238000006467 substitution reaction Methods 0.000 claims description 2
- 238000002054 transplantation Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 description 11
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202062984618P | 2020-03-03 | 2020-03-03 | |
| US62/984,618 | 2020-03-03 | ||
| US202163146447P | 2021-02-05 | 2021-02-05 | |
| US63/146,447 | 2021-02-05 | ||
| PCT/US2021/020513 WO2021178432A1 (en) | 2020-03-03 | 2021-03-02 | Rna-guided genome recombineering at kilobase scale |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2023515670A JP2023515670A (ja) | 2023-04-13 |
| JPWO2021178432A5 true JPWO2021178432A5 (https=) | 2024-03-07 |
| JP2023515670A5 JP2023515670A5 (https=) | 2024-03-07 |
Family
ID=77614129
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022552549A Pending JP2023515670A (ja) | 2020-03-03 | 2021-03-02 | キロ塩基スケールでのｒｎａガイドゲノムリコンビニアリング |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20230091242A1 (https=) |
| EP (1) | EP4114845A4 (https=) |
| JP (1) | JP2023515670A (https=) |
| KR (1) | KR20220151175A (https=) |
| CN (1) | CN115667283A (https=) |
| AU (1) | AU2021231769A1 (https=) |
| BR (1) | BR112022017196A2 (https=) |
| CA (1) | CA3173526A1 (https=) |
| IL (1) | IL296057A (https=) |
| MX (1) | MX2022010835A (https=) |
| WO (1) | WO2021178432A1 (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4396340A4 (en) * | 2021-09-01 | 2025-09-24 | Univ Leland Stanford Junior | RNA-GUIDED GENOME RECOMBINATION AT THE KILOBASE SCALE |
| JP2025505732A (ja) * | 2022-02-10 | 2025-02-28 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | キロ塩基スケールでのｒｎａガイドゲノムリコンビニアリング |
| WO2023154892A1 (en) * | 2022-02-10 | 2023-08-17 | Possible Medicines Llc | Rna-guided genome recombineering at kilobase scale |
| WO2024168265A1 (en) * | 2023-02-10 | 2024-08-15 | Possible Medicines Llc | Aav delivery of rna guided recombination system |
| WO2024168253A1 (en) * | 2023-02-10 | 2024-08-15 | Possible Medicines Llc | Delivery of an rna guided recombination system |
| CN120555441B (zh) * | 2025-05-29 | 2026-04-17 | 西北农林科技大学 | 基于CtIP蛋白提高CRISPR/Cas9介导的外源基因整合效率的DNA适配体及其应用 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU752105B2 (en) * | 1997-12-05 | 2002-09-05 | Europaisches Laboratorium Fur Molekularbiologie (Embl) | Novel dna cloning method |
| US9388430B2 (en) * | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| WO2016022363A2 (en) * | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| EP3265559B1 (en) * | 2015-03-03 | 2021-01-06 | The General Hospital Corporation | Engineered crispr-cas9 nucleases with altered pam specificity |
| WO2016205759A1 (en) * | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
| US11479793B2 (en) * | 2015-07-15 | 2022-10-25 | Rutgers, The State University Of New Jersey | Nuclease-independent targeted gene editing platform and uses thereof |
| WO2019089910A1 (en) * | 2017-11-01 | 2019-05-09 | Ohio State Innovation Foundation | Highly compact cas9-based transcriptional regulators for in vivo gene regulation |
| KR20210152597A (ko) * | 2017-12-22 | 2021-12-15 | (주)지플러스생명과학 | 키메라 게놈 조작 분자 및 방법 |
| EP4396340A4 (en) * | 2021-09-01 | 2025-09-24 | Univ Leland Stanford Junior | RNA-GUIDED GENOME RECOMBINATION AT THE KILOBASE SCALE |
-
2021
- 2021-03-02 IL IL296057A patent/IL296057A/en unknown
- 2021-03-02 CA CA3173526A patent/CA3173526A1/en active Pending
- 2021-03-02 EP EP21764351.9A patent/EP4114845A4/en active Pending
- 2021-03-02 WO PCT/US2021/020513 patent/WO2021178432A1/en not_active Ceased
- 2021-03-02 KR KR1020227033540A patent/KR20220151175A/ko active Pending
- 2021-03-02 AU AU2021231769A patent/AU2021231769A1/en active Pending
- 2021-03-02 BR BR112022017196A patent/BR112022017196A2/pt unknown
- 2021-03-02 MX MX2022010835A patent/MX2022010835A/es unknown
- 2021-03-02 JP JP2022552549A patent/JP2023515670A/ja active Pending
- 2021-03-02 US US17/905,457 patent/US20230091242A1/en active Pending
- 2021-03-02 CN CN202180033011.8A patent/CN115667283A/zh active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2022201038B2 (en) | Compositions and methods for promoting homology directed repair | |
| US10597648B2 (en) | Engineered cascade components and cascade complexes | |
| US11840694B2 (en) | Truncated CRISPR-Cas proteins for DNA targeting | |
| CN111757889A (zh) | 新型CRISPR/Cas12f酶和系统 | |
| CN113015798B (zh) | CRISPR-Cas12a酶和系统 | |
| KR100865602B1 (ko) | 스캐폴드 구조의 ｃ-타입 렉틴 유사 도메인을 가지는단백질의 조합 라이브러리 | |
| IL309801B1 (en) | Using nucleosome interacting protein domains to enhance targeted genome modification | |
| US20130143225A1 (en) | Variant reverse transcriptase | |
| KR20240055073A (ko) | 클래스 ii, v형 crispr 시스템 | |
| CN110520163A (zh) | 独立于dna双链断裂的靶向基因编辑平台及其用途 | |
| KR20180015731A (ko) | 캄필로박터 제주니 crispr/cas 시스템 유래 rgen을 이용한 유전체 교정 | |
| JP2002537790A (ja) | 核酸の組換えクローニングにおける使用のための組成物および方法 | |
| US20240254464A1 (en) | Cleavage-inactive cas12f1, cleavage-inactive cas12f1-based fusion protein, crispr gene-editing system comprising same, and preparation method and use thereof | |
| WO2003091429A1 (en) | Antimicrobial polypeptide and utizliation thereof | |
| JPWO2022056324A5 (https=) | ||
| JPWO2020160514A5 (https=) | ||
| Firth et al. | A lipoprotein signal peptide encoded by the staphylococcal conjugative plasmid pSK41 exhibits an activity resembling that of Enterococcus faecalis pheromone cAD1 | |
| JPWO2021178432A5 (https=) | ||
| WO2024078645A3 (zh) | Cas蛋白及其应用 | |
| JPWO2023169410A5 (https=) | ||
| WO2007129087A1 (en) | Plasmid curing | |
| US8003348B2 (en) | Method for the mass expression of an antimicrobial peptide by co-expression of a basic antimicrobial peptide and an acidic peptide using a translational coupling system | |
| JPWO2023154877A5 (https=) | ||
| KR101286733B1 (ko) | 세포표면에서 발현되는 항균 펩타이드 다중합체 | |
| US20210139917A1 (en) | Peptide macrocyclization enzyme |