AU2021231769A1 - RNA-guided genome recombineering at kilobase scale - Google Patents

RNA-guided genome recombineering at kilobase scale Download PDF

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AU2021231769A1
AU2021231769A1 AU2021231769A AU2021231769A AU2021231769A1 AU 2021231769 A1 AU2021231769 A1 AU 2021231769A1 AU 2021231769 A AU2021231769 A AU 2021231769A AU 2021231769 A AU2021231769 A AU 2021231769A AU 2021231769 A1 AU2021231769 A1 AU 2021231769A1
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protein
sequence
aptamer
nucleic acid
seq
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AU2021231769A
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Le Cong
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Leland Stanford Junior University
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Leland Stanford Junior University
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2800/00—Nucleic acids vectors
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2021231769A 2020-03-03 2021-03-02 RNA-guided genome recombineering at kilobase scale Pending AU2021231769A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202062984618P 2020-03-03 2020-03-03
US62/984,618 2020-03-03
US202163146447P 2021-02-05 2021-02-05
US63/146,447 2021-02-05
PCT/US2021/020513 WO2021178432A1 (en) 2020-03-03 2021-03-02 Rna-guided genome recombineering at kilobase scale

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AU2021231769A1 true AU2021231769A1 (en) 2022-09-29

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US (1) US20230091242A1 (https=)
EP (1) EP4114845A4 (https=)
JP (1) JP2023515670A (https=)
KR (1) KR20220151175A (https=)
CN (1) CN115667283A (https=)
AU (1) AU2021231769A1 (https=)
BR (1) BR112022017196A2 (https=)
CA (1) CA3173526A1 (https=)
IL (1) IL296057A (https=)
MX (1) MX2022010835A (https=)
WO (1) WO2021178432A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2024002482A (es) * 2021-09-01 2024-09-19 Univ Leland Stanford Junior Recombinacion del genoma guiada por arn en escala de kilobase.
WO2023154892A1 (en) * 2022-02-10 2023-08-17 Possible Medicines Llc Rna-guided genome recombineering at kilobase scale
JP2025505732A (ja) * 2022-02-10 2025-02-28 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー キロ塩基スケールでのｒｎａガイドゲノムリコンビニアリング
WO2024168253A1 (en) * 2023-02-10 2024-08-15 Possible Medicines Llc Delivery of an rna guided recombination system
WO2024168265A1 (en) * 2023-02-10 2024-08-15 Possible Medicines Llc Aav delivery of rna guided recombination system
CN120555441B (zh) * 2025-05-29 2026-04-17 西北农林科技大学 基于CtIP蛋白提高CRISPR/Cas9介导的外源基因整合效率的DNA适配体及其应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6509156B1 (en) * 1997-12-05 2003-01-21 Europaisches Laboratorium Fur Molekularoiologie (Embl) DNA cloning method relying on the E. coli recE/recT recombination system
US9322037B2 (en) * 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US10077453B2 (en) * 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
KR20230156800A (ko) * 2015-03-03 2023-11-14 더 제너럴 하스피탈 코포레이션 변경된 PAM 특이성을 갖는 조작된 CRISPR-Cas9 뉴클레아제
WO2016205759A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation
JP7044373B2 (ja) * 2015-07-15 2022-03-30 ラトガース，ザ ステート ユニバーシティ オブ ニュージャージー ヌクレアーゼ非依存的な標的化遺伝子編集プラットフォームおよびその用途
WO2019089910A1 (en) * 2017-11-01 2019-05-09 Ohio State Innovation Foundation Highly compact cas9-based transcriptional regulators for in vivo gene regulation
EP3728589A4 (en) * 2017-12-22 2021-11-03 G+Flas Life Sciences CHEMICAL GENOMIC ENGINEERING MOLECULES AND PROCESSES
MX2024002482A (es) * 2021-09-01 2024-09-19 Univ Leland Stanford Junior Recombinacion del genoma guiada por arn en escala de kilobase.

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Publication number Publication date
BR112022017196A2 (pt) 2022-10-25
WO2021178432A9 (en) 2021-10-28
WO2021178432A1 (en) 2021-09-10
EP4114845A1 (en) 2023-01-11
CA3173526A1 (en) 2021-09-10
EP4114845A4 (en) 2024-03-06
US20230091242A1 (en) 2023-03-23
KR20220151175A (ko) 2022-11-14
JP2023515670A (ja) 2023-04-13
MX2022010835A (es) 2022-09-29
IL296057A (en) 2022-10-01
CN115667283A (zh) 2023-01-31

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