JP2023515670A - キロ塩基スケールでのｒｎａガイドゲノムリコンビニアリング - Google Patents

キロ塩基スケールでのｒｎａガイドゲノムリコンビニアリング Download PDF

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JP2023515670A
JP2023515670A JP2022552549A JP2022552549A JP2023515670A JP 2023515670 A JP2023515670 A JP 2023515670A JP 2022552549 A JP2022552549 A JP 2022552549A JP 2022552549 A JP2022552549 A JP 2022552549A JP 2023515670 A JP2023515670 A JP 2023515670A
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sequence
protein
nucleic acid
aptamer
cas9
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JPWO2021178432A5 (https=
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リー コン，
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Leland Stanford Junior University
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Leland Stanford Junior University
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    • C12N15/09—Recombinant DNA-technology
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
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    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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JP2022552549A 2020-03-03 2021-03-02 キロ塩基スケールでのｒｎａガイドゲノムリコンビニアリング Pending JP2023515670A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202062984618P 2020-03-03 2020-03-03
US62/984,618 2020-03-03
US202163146447P 2021-02-05 2021-02-05
US63/146,447 2021-02-05
PCT/US2021/020513 WO2021178432A1 (en) 2020-03-03 2021-03-02 Rna-guided genome recombineering at kilobase scale

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JP2023515670A true JP2023515670A (ja) 2023-04-13
JPWO2021178432A5 JPWO2021178432A5 (https=) 2024-03-07
JP2023515670A5 JP2023515670A5 (https=) 2024-03-07

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US (1) US20230091242A1 (https=)
EP (1) EP4114845A4 (https=)
JP (1) JP2023515670A (https=)
KR (1) KR20220151175A (https=)
CN (1) CN115667283A (https=)
AU (1) AU2021231769A1 (https=)
BR (1) BR112022017196A2 (https=)
CA (1) CA3173526A1 (https=)
IL (1) IL296057A (https=)
MX (1) MX2022010835A (https=)
WO (1) WO2021178432A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4396340A4 (en) * 2021-09-01 2025-09-24 Univ Leland Stanford Junior RNA-GUIDED GENOME RECOMBINATION AT THE KILOBASE SCALE
JP2025505732A (ja) * 2022-02-10 2025-02-28 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー キロ塩基スケールでのｒｎａガイドゲノムリコンビニアリング
WO2023154892A1 (en) * 2022-02-10 2023-08-17 Possible Medicines Llc Rna-guided genome recombineering at kilobase scale
WO2024168265A1 (en) * 2023-02-10 2024-08-15 Possible Medicines Llc Aav delivery of rna guided recombination system
WO2024168253A1 (en) * 2023-02-10 2024-08-15 Possible Medicines Llc Delivery of an rna guided recombination system
CN120555441B (zh) * 2025-05-29 2026-04-17 西北农林科技大学 基于CtIP蛋白提高CRISPR/Cas9介导的外源基因整合效率的DNA适配体及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018506987A (ja) * 2015-03-03 2018-03-15 ザ ジェネラル ホスピタル コーポレイション 変更ＰＡＭ特異性を有する遺伝子操作ＣＲＩＳＰＲ−Ｃａｓ９ヌクレアーゼ
JP2018520686A (ja) * 2015-07-15 2018-08-02 ラトガース，ザ ステート ユニバーシティ オブ ニュージャージー ヌクレアーゼ非依存的な標的化遺伝子編集プラットフォームおよびその用途

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AU752105B2 (en) * 1997-12-05 2002-09-05 Europaisches Laboratorium Fur Molekularbiologie (Embl) Novel dna cloning method
US9388430B2 (en) * 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
WO2016022363A2 (en) * 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
WO2016205759A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation
WO2019089910A1 (en) * 2017-11-01 2019-05-09 Ohio State Innovation Foundation Highly compact cas9-based transcriptional regulators for in vivo gene regulation
KR20210152597A (ko) * 2017-12-22 2021-12-15 (주)지플러스생명과학 키메라 게놈 조작 분자 및 방법
EP4396340A4 (en) * 2021-09-01 2025-09-24 Univ Leland Stanford Junior RNA-GUIDED GENOME RECOMBINATION AT THE KILOBASE SCALE

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
JP2018506987A (ja) * 2015-03-03 2018-03-15 ザ ジェネラル ホスピタル コーポレイション 変更ＰＡＭ特異性を有する遺伝子操作ＣＲＩＳＰＲ−Ｃａｓ９ヌクレアーゼ
JP2018520686A (ja) * 2015-07-15 2018-08-02 ラトガース，ザ ステート ユニバーシティ オブ ニュージャージー ヌクレアーゼ非依存的な標的化遺伝子編集プラットフォームおよびその用途

Non-Patent Citations (1)

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Title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. Vol.85, Issue 21, JPN6025010055, 2019, pages 01367 - 19, ISSN: 0005546872 *

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IL296057A (en) 2022-10-01
EP4114845A1 (en) 2023-01-11
MX2022010835A (es) 2022-09-29
AU2021231769A1 (en) 2022-09-29
WO2021178432A1 (en) 2021-09-10
KR20220151175A (ko) 2022-11-14
EP4114845A4 (en) 2024-03-06
CN115667283A (zh) 2023-01-31
US20230091242A1 (en) 2023-03-23
WO2021178432A9 (en) 2021-10-28
BR112022017196A2 (pt) 2022-10-25
CA3173526A1 (en) 2021-09-10

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