JPWO2021102390A5 - - Google Patents
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- Publication number
- JPWO2021102390A5 JPWO2021102390A5 JP2022529540A JP2022529540A JPWO2021102390A5 JP WO2021102390 A5 JPWO2021102390 A5 JP WO2021102390A5 JP 2022529540 A JP2022529540 A JP 2022529540A JP 2022529540 A JP2022529540 A JP 2022529540A JP WO2021102390 A5 JPWO2021102390 A5 JP WO2021102390A5
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- parapalindromic
- recombinase polypeptide
- nucleic acid
- region
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229920001184 polypeptide Polymers 0.000 claims description 67
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 67
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 67
- 102000018120 Recombinases Human genes 0.000 claims description 63
- 108010091086 Recombinases Proteins 0.000 claims description 63
- 239000002773 nucleotide Substances 0.000 claims description 58
- 125000003729 nucleotide group Chemical group 0.000 claims description 58
- 150000007523 nucleic acids Chemical class 0.000 claims description 50
- 108020004707 nucleic acids Proteins 0.000 claims description 44
- 102000039446 nucleic acids Human genes 0.000 claims description 44
- 210000003527 eukaryotic cell Anatomy 0.000 claims description 33
- 238000000034 method Methods 0.000 claims description 29
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 24
- 238000012217 deletion Methods 0.000 claims description 16
- 230000037430 deletion Effects 0.000 claims description 16
- 210000005260 human cell Anatomy 0.000 claims description 16
- 238000003780 insertion Methods 0.000 claims description 16
- 230000037431 insertion Effects 0.000 claims description 16
- 238000012986 modification Methods 0.000 claims description 16
- 230000004048 modification Effects 0.000 claims description 16
- 238000006467 substitution reaction Methods 0.000 claims description 16
- 210000004962 mammalian cell Anatomy 0.000 claims description 11
- 210000004027 cell Anatomy 0.000 claims description 10
- 238000003556 assay Methods 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 150000001413 amino acids Chemical class 0.000 claims description 2
- 210000001236 prokaryotic cell Anatomy 0.000 claims description 2
- 239000013603 viral vector Substances 0.000 claims 3
- 239000013607 AAV vector Substances 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 108020004999 messenger RNA Proteins 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 24
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962939525P | 2019-11-22 | 2019-11-22 | |
| US62/939,525 | 2019-11-22 | ||
| US202063039309P | 2020-06-15 | 2020-06-15 | |
| US63/039,309 | 2020-06-15 | ||
| US202063068402P | 2020-08-21 | 2020-08-21 | |
| US63/068,402 | 2020-08-21 | ||
| PCT/US2020/061705 WO2021102390A1 (en) | 2019-11-22 | 2020-11-22 | Recombinase compositions and methods of use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2023502473A JP2023502473A (ja) | 2023-01-24 |
| JPWO2021102390A5 true JPWO2021102390A5 (de) | 2023-12-18 |
Family
ID=75980912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022529540A Pending JP2023502473A (ja) | 2019-11-22 | 2020-11-22 | リコンビナーゼ組成物及び使用方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20230131847A1 (de) |
| EP (1) | EP4061940A4 (de) |
| JP (1) | JP2023502473A (de) |
| CN (1) | CN115397984A (de) |
| CA (1) | CA3162499A1 (de) |
| WO (1) | WO2021102390A1 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112021003380A2 (pt) | 2018-08-28 | 2021-05-18 | Flagship Pioneering Innovations Vi, Llc | métodos e composições para modulação de um genoma |
| EP4114941A4 (de) | 2020-03-04 | 2024-10-16 | Flagship Pioneering Innovations Vi Llc | Verbesserte verfahren und zusammensetzungen zur modulierung eines genoms |
| BR112023001648A2 (pt) | 2020-07-27 | 2023-04-04 | Anjarium Biosciences Ag | Moléculas de dna de fita dupla, veículo de entrega e método para preparar uma molécula de dna com extremidade em grampo |
| JP2024533311A (ja) | 2021-09-08 | 2024-09-12 | フラッグシップ パイオニアリング イノベーションズ シックス，エルエルシー | ゲノムを調節するための方法及び組成物 |
| EP4416279A1 (de) * | 2021-10-14 | 2024-08-21 | Asimov, Inc. | Integrasen, landepadarchitekturen und manipulierte zellen damit |
| EP4448742A1 (de) * | 2021-12-17 | 2024-10-23 | Massachusetts Institute Of Technology | Programmierbare insertionsansätze zur rekrutierung der reversen transkriptase |
| WO2024081738A2 (en) * | 2022-10-11 | 2024-04-18 | The Trustees Of Columbia University In The City Of New York | Compositions, methods, and systems for dna modification |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8304233B2 (en) * | 2002-06-04 | 2012-11-06 | Poetic Genetics, Llc | Methods of unidirectional, site-specific integration into a genome, compositions and kits for practicing the same |
| FR2850668B1 (fr) * | 2003-01-31 | 2005-04-08 | Centre Nat Rech Scient | Elements genetiques mobiles appartenant a la famille mariner chez les eucaryotes hydrothermaux |
| US9034650B2 (en) * | 2005-02-02 | 2015-05-19 | Intrexon Corporation | Site-specific serine recombinases and methods of their use |
| WO2008100424A2 (en) * | 2007-02-09 | 2008-08-21 | University Of Hawaii | Animals and cells with genomic target sites for transposase-mediated transgenesis |
| EP2527448A1 (de) * | 2011-05-23 | 2012-11-28 | Novozymes A/S | Gleichzeitige standortspezifische Integrationen von mehreren Genkopien in faserige Pilze |
| JP2022542839A (ja) * | 2019-07-19 | 2022-10-07 | フラッグシップ パイオニアリング イノベーションズ シックス，エルエルシー | リコンビナーゼ組成物及び使用方法 |
-
2020
- 2020-11-22 WO PCT/US2020/061705 patent/WO2021102390A1/en unknown
- 2020-11-22 EP EP20890444.1A patent/EP4061940A4/de active Pending
- 2020-11-22 JP JP2022529540A patent/JP2023502473A/ja active Pending
- 2020-11-22 CA CA3162499A patent/CA3162499A1/en active Pending
- 2020-11-22 CN CN202080094315.0A patent/CN115397984A/zh active Pending
-
2022
- 2022-05-20 US US17/749,788 patent/US20230131847A1/en active Pending
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