JPWO2017022722A1 - Fatigue reducing agent - Google Patents

Abstract

The present invention provides a fatigue-reducing agent comprising a bee-derived propolis belonging to the genus Bee as an active ingredient.

Description

  The present invention relates to a fatigue reducing agent.

  Propolis is a wax-like composition obtained by solidifying a resin collected by various honey bee insects from various plants, and has been used as a food for a long time, and is known to have various physiological activities. For example, Patent Document 1 discloses that a mixture of guarana water extract, propolis water extract, DHA, phosphatidylserine, phosphatidylcholine and vitamin E has an improving effect on dementia, chronic fatigue syndrome and the like. Is disclosed.

  As the bee of the honeybee family that produces propolis, those belonging to the genus Apis, the genus Trigona, or the genus Melipona are known. Patent Document 2 discloses an anti-fatigue agent containing, as an active ingredient, a propolis extract produced by hedgehog bees.

JP 2008-50331 A JP-A-2005-281182

  In patent document 1, the effect regarding the fatigue only by propolis is not confirmed. Moreover, in patent document 1, only the evaluation by a questionnaire is made and the influence on a body is not confirmed by the objective method.

  The honeybees described in Patent Document 2 are literally bees that have degenerated their needles in the process of evolution, and have a long queen bee life of more than 10 years. The nest itself is made of propolis and has high antibacterial properties. It is characterized by fewer illnesses because it makes honey. However, the hedgehog bees have a property that colonies collapse when exposed to low temperatures, so their habitat is limited to the tropics and cannot be widely used. Moreover, in patent document 2, the fatigue reduction effect of the propolis derived from the bee belonging to the genus Bee is not found.

  An object of the present invention is to provide a novel use of a propolis derived from a bee belonging to the genus Bee.

  The present invention provides a fatigue relieving agent containing bee-derived propolis belonging to the genus Bee or a processed product thereof as an active ingredient.

  The fatigue reducing agent is preferably for oral use.

  The fatigue reducing agent of the present invention can be used as a food composition for reducing fatigue. The food composition includes foods, health foods, functional labeling foods (functional foods), dietary supplements, supplements and foods for specified health use. Propolis has been conventionally used as food and the like, and safety to living bodies has been established. Therefore, the said food composition for fatigue reduction can also be ingested continuously over a long period of time.

  The present invention can also be considered as a propolis derived from a bee belonging to the genus Bee or a processed product thereof for use in reducing fatigue. In addition, the present invention can be regarded as an agent containing bee-derived propolis belonging to the genus Bee or a processed product thereof as an active ingredient for use in reducing fatigue.

  The present invention can also be regarded as the use of bee-derived propolis belonging to the genus Bee or a processed product thereof in the production of a fatigue reducing agent.

  The present invention can also be regarded as a method for reducing fatigue, comprising administering an effective amount of a propolis derived from a bee belonging to the genus Bee or a processed product thereof to a subject in need thereof.

  ADVANTAGE OF THE INVENTION According to this invention, the fatigue reducing agent which contains a propolis or its processed material as an active ingredient can be provided.

It is a graph which shows a time-dependent change of the oxygen intake per body weight. It is a graph which shows a time-dependent change of exercise intensity. It is a graph which shows a time-dependent change of minute ventilation. It is a graph which shows a time-dependent change of the maximum expiration rate. It is a graph which shows a time-dependent change of energy consumption. It is a graph which shows the time-dependent change of the anti-fatigue feeling after walking exercise. It is a graph which shows the time-dependent change of the fatigue feeling felt in everyday life.

  Hereinafter, embodiments for carrying out the present invention will be described in detail. However, the present invention is not limited to the following embodiments.

  The fatigue reducing agent of the present invention contains propolis or a processed product thereof as an active ingredient.

  Propolis is a resinous or waxy substance that forms the nest wall of the beehive. Propolis is known to contain cinnamic acid derivatives, flavonoids, vitamins, minerals and the like.

  The propolis used in this embodiment is derived from a bee belonging to the genus Bee family. Nine species such as Apis mellifera and Apis cerana belong to the genus Bee, and propolis derived from any species may be used. Among the bees, it is preferable to use a propolis derived from the honey bee. There are 24-28 subspecies in honeybees, and propolis derived from any subspecies may be used. In particular, it is preferable to use a propolis derived from an African bee honey bee that is a hybrid of an A. mellifera scutellata, which is one of the subspecies of honey bee, and another European subspecies of honey bee.

  Propolis can be obtained, for example, as a beekeeping product according to a conventional method. Propolis may be derived from any origin, such as from Japan, Brazil, China, European countries, Oceania, USA, and the like. Further, the propolis may be derived from any plant such as derived from aleklin, derived from eucalyptus, derived from poplar, or derived from a plant belonging to the genus Kurusia. The propolis may have any rank such as brown, red, yellow, green, super green, ultra green and the like. The propolis is preferably a propolis derived from an African bee honey bee produced in Brazil from the viewpoint of high physiological activity and availability.

  The propolis may be, for example, a propolis bulk. The processed propolis may be obtained by, for example, subjecting a raw propolis mass to processing such as grinding, supercritical extraction, water or hydrophilic organic solvent extraction, concentration or pulverization of the extract, and granulation of powder. . Among these, the hydrophilic organic solvent extract obtained by the hydrophilic organic solvent extraction of propolis is preferable because the active ingredient of propolis is efficiently and well-balanced in a short time. As the hydrophilic organic solvent used for extraction, ethanol is preferred.

  A commercially available product may be used as propolis or a processed product of propolis. Specific examples of commercially available products including propolis derived from honey bees or processed products of propolis include, for example, Propolis 300 of Yamada Bee Farm Co., Ltd., Propolis fluid 30 (containing Brazilian propolis), propolis help, propolis granules, propolis granules APC , Propolis Mild, Propolis Drink, Neo Propolis Granules, Propolis Granules, Propolis Fluid, Propolis Mild Fluid, Eucalyptus, Made by Morikawa Kendo Co., Ltd. Labeille Propolis (Liquid Type), Labeille Propolis (Capsule Type), Labeille propolis honey and the like.

  In the fatigue reducing agent according to the present embodiment, the above-described propolis bulk or processed product of propolis may be used in only one form, or two or more forms may be used in combination. The content of propolis and the processed product in the fatigue reducing agent may be, for example, 0.002% by mass to 50% by mass in terms of solid content, and 0.01% by mass to 45% by mass with respect to the total amount of the fatigue reducing agent. Or 0.1 mass% or more and 40 mass% or less.

  Since the fatigue reducing agent according to the present embodiment contains propolis or a processed product thereof as an active ingredient, it can reduce fatigue during exercise and daily life by ingestion. Specifically, by ingesting the fatigue reducing agent according to the present embodiment, as shown in the examples described later, when performing the same amount of exercise, oxygen intake, minute ventilation, maximum Expiratory speed, exercise intensity, energy consumption and the like can be reduced, and physical fatigue during exercise and daily life can be reduced. Among these, the effect of reducing minute ventilation and fatigue feeling in daily life is high. That is, by taking the fatigue reducing agent according to the present embodiment, the motor function can be improved, and the physical burden during exercise and daily life can be reduced. Therefore, the fatigue reducing agent according to the present embodiment can also be referred to as a motor function improving agent.

  The subject ingesting the fatigue reducing agent according to the present embodiment may be any person who desires the fatigue reducing effect, and is particularly effective for those who tend to lack exercise momentarily. For example, it is effective for middle-aged and elderly people aged 40 to 65 who are worried about lack of exercise and fatigue.

  The fatigue reducing agent according to the present embodiment has a remarkable effect at the latest after about 4 weeks from the start of daily continuous intake, and has a higher effect by continuing the intake. In general, it is considered that exercise function can be improved by daily training such as fitness, aerobic exercise, etc., and ease of fatigue can be reduced. By ingesting, there is an effect of improving motor function, reducing fatigue, etc. at an early stage of about 4 weeks to 8 weeks after the start of ingestion, even without particularly training. Therefore, it is particularly suitable for those who want to improve motor function early and reduce fatigue without relying on training. The intake of the fatigue reducing agent according to the present embodiment may be performed in parallel with the training.

  The fatigue reducing agent according to the present embodiment may contain only propolis which is an active ingredient or a processed product of propolis, and may further contain other ingredients as long as the effects of the present invention are not hindered. Good. Examples of other components include pharmaceutically acceptable components (for example, excipients, binders, lubricants, disintegrants, emulsifiers, surfactants, bases, solubilizers, suspending agents). , Food-acceptable ingredients (eg minerals, vitamins, flavonoids, quinones, polyphenols, amino acids, nucleic acids, essential fatty acids, cooling agents, binders, sweeteners, disintegrants, lubricants, colorants Fragrances, stabilizers, preservatives, sustained release regulators, surfactants, solubilizers, wetting agents).

  The fatigue reducing agent according to the present embodiment can be used at a dose of 1 mg or more and 550 mg or less per day for an adult with a body weight of 60 kg in terms of the amount of active ingredient, and preferably used at a dose of 3 mg or more and 500 mg or less. More preferably, it is used at a dose of 7 mg or more and 400 mg or less. The volume can be appropriately set within the above-mentioned range according to factors such as the health condition of the person who ingests, the administration method, and combinations with other agents. In addition, the fatigue reducing agent according to the present embodiment has a sufficient fatigue reducing effect even if the dose is 460 mg or less, 300 mg or less, or 230 mg or less in terms of an active ingredient per day for an adult weighing 60 kg.

  The fatigue reducing agent according to this embodiment may be administered orally (ingested) or parenterally. The fatigue reducing agent according to the present embodiment may be administered once a day as long as the amount of active ingredient per day is within the above-mentioned range, or twice a day, three times a day, etc. May be administered separately.

  The fatigue reducing agent according to this embodiment is preferably administered orally. By administering orally, the functional component of propolis can be efficiently taken into the body, and higher physiological activity can be brought about. Therefore, the fatigue reducing agent according to this embodiment is preferably for oral use.

  The fatigue reducing agent according to the present embodiment may have any shape such as a solid, a liquid, and a paste. The form of the fatigue reducing agent according to the present embodiment may be, for example, an uncoated tablet, a sugar-coated tablet, a granule, a powder, a tablet, and a capsule (hard capsule, soft capsule, seamless capsule). The fatigue reducing agent according to the present embodiment can be prepared, for example, by mixing propolis which is an active ingredient or a processed product of propolis and, if necessary, other ingredients and forming the dosage form.

  The fatigue reducing agent according to the present embodiment can be used as a pharmaceutical, a quasi drug, and a food composition itself, and added to a pharmaceutical, a quasi drug, and a food composition. The food composition preferably emphasizes the tertiary function of the food (physical condition control function, that is, fatigue reduction function, motor function improvement function, etc.). Examples of food compositions in which the tertiary function of food is emphasized include health foods, functional labeling foods (functional foods), nutritional supplements, supplements, and foods for specified health use.

  The pharmaceutical, quasi-drug or food composition comprising the fatigue-reducing agent according to the present embodiment, or the pharmaceutical, quasi-drug or food composition containing the fatigue-reducing agent according to the present embodiment is used for reducing fatigue and exercising function. It may be for improvement. In addition, the medicine, quasi drug, or food may be provided with an indication such as reducing fatigue and improving exercise function.

  The content of the fatigue reducing agent according to this embodiment in the pharmaceutical, quasi-drug, and food composition is such that the amount of the active ingredient taken per day is within the above-described range. What is necessary is just to set suitably according to the kind of thing.

  When the fatigue reducing agent according to the present embodiment is used as the food composition itself or added to the food composition, the form of the food composition is not particularly limited. For example, beverages (coffee, juice, tea beverage, Soft drinks such as jelly drinks, milk drinks, lactic acid bacteria drinks, yogurt drinks, carbonated drinks, etc., spreads (such as custard cream), pastes (such as fruit paste), pastries (chocolate, donuts, pies, cream puffs, gums, Jelly, candy, cookies, cakes, pudding, etc.), Japanese sweets (Daifuku, rice cakes, buns, castella, anmitsu, mutton etc.), ice confections (ice cream, popsicle, sherbet, etc.), foods (curry, beef bowl, Miscellaneous cooking, miso soup, soup, meat sauce, pasta, pickles, jam, etc.), seasonings (dressing, sprinkles, umami) Seasonings, may be a arsenide) of soup.

  The fatigue reducing agent according to the present embodiment is a food composition in which the tertiary function of food is emphasized (for example, health food, functional indication food (functional food), dietary supplement, supplement, or food for specified health use) itself. In addition to the above-described food composition, the form of the food composition in which the tertiary function of the food is emphasized is used, for example, in addition to the food composition in which the tertiary function of the food is emphasized. , Uncoated tablets, dragees, granules, powders, tablets, capsules (hard capsules, soft capsules, seamless capsules).

  When the fatigue reducing agent according to the present embodiment is used as a pharmaceutical product or quasi-drug itself or added to the pharmaceutical product or quasi-drug, the form of the pharmaceutical product or quasi-drug is not particularly limited. Tablets, dragees, granules, powders, tablets, capsules (hard capsules, soft capsules, seamless capsules) may be used.

  The manufacturing method of the pharmaceutical, the quasi-drug, or the food composition to which the fatigue reducing agent according to this embodiment is added is not particularly limited, and can be appropriately followed by a known method. For example, by mixing the fatigue reducing agent according to the present embodiment with an intermediate product or final product in the manufacturing process of a pharmaceutical product, quasi-drug or food composition, the pharmaceutical product, quasi-drug or A food composition can be obtained.

  The present invention described above can also be regarded as propolis or a processed product thereof for use in reducing fatigue. The present invention can also be regarded as an agent containing propolis or a processed product thereof as an active ingredient for use in reducing fatigue. The present invention can also be regarded as the use of propolis or a processed product thereof in the manufacture of a fatigue reducing agent.

  The present invention described above can also be regarded as a method for reducing fatigue, including administering an effective amount of propolis or a processed product thereof to a subject in need thereof.

  Hereinafter, the present invention will be described more specifically based on examples. However, the present invention is not limited to the following examples.

<Test method>
Forty-five men and women aged 40 to 65 who replied “I do not usually exercise very much” in the questionnaire questions were selected as subjects. The group was divided into three groups of 15 persons so that the age and gender ratios were as equal as possible, and were divided into (A) propolis intake and walking exercise group, (B) propolis intake group, and (C) walking exercise group, respectively.

  Group (A) and Group (B) are soft capsules of propolis extract (trade name “Propolis 300”, manufactured by Yamada Bee Farm, Brazilian propolis extract, derived from African bee honeybee, 75.6 mg / grain) 3 times a day. Grain was taken daily. In the groups (A) and (C), the walking exercise for 30 minutes or more / day was continued for 3 weeks or more for 12 weeks. Immediately before the start of the study (week 0), at the 4th and 8th weeks after the start of the study, the following breath gas metabolism measurements are conducted, and a questionnaire is conducted at the 12th week of the study to examine the effects on health and physical fitness. It was.

[Measurement of breath gas metabolism]
Each subject performed a walking exercise on the treadmill for 15 minutes while wearing a breath gas metabolism measurement mask. The walking movement was resting for 2 minutes, warming up for 1 minute, speeds of 60 m, 80 m and 100 m for 3 minutes each for 9 minutes in total, cooling down for 1 minute and recovery period of 2 minutes for a total of 15 minutes.

  Using an exhaled gas metabolism measuring device (METALYZER 3B, manufactured by Coletex), the oxygen amount and carbon dioxide concentration in the exhaled breath during the walking exercise were measured over time. In addition, the respiratory rate during walking exercise (number of breaths during walking exercise), minute ventilation (respiratory rate during walking exercise), maximum expiratory rate (maximum respiratory rate during walking exercise) and energy consumption were measured. . About oxygen consumption and energy consumption, the value per body weight of each test subject was calculated. In addition, the maximum expiratory speed indicates that the exercise can be performed without raising the breath as the value is small, and the energy consumption indicates that the exercise can be performed at the same level with less energy as the value is small. Is.

  The exercise intensity was calculated from the measured carbon dioxide emission and oxygen intake. The exercise intensity is a value calculated from the amount of oxygen intake during exercise and the amount of oxygen intake at rest. The larger the value, the greater the load on the exercise performed.

  The average value for 15 minutes during walking exercise was calculated for each of the above items. The value of each item immediately before the start of the test (week 0) was used as a reference (100%), and changes over time after 4 weeks were recorded. The results are shown in FIGS. In the figure, the * mark indicates that the p value is less than 0.05 at 0 weeks.

〔questionnaire〕
Immediately before the start of the test (week 0) and 12 weeks after the start of the test, a questionnaire was conducted on the anti-fatigue feeling after walking exercise and the feeling of fatigue felt in daily life. Anti-fatigue after walking exercise is evaluated by visual evaluation scale (VAS) method, with the worst feeling exhausted so that nothing can be done being evaluated as 0, and the best feeling not feeling fatigue at all being evaluated as 100. Obtained. The feeling of fatigue felt in daily life was evaluated on a scale of 0 when the person felt that he / she did not feel, and the degree of fatigue when he / she felt it (1: light, 5: heavy). The results are shown in FIGS. In the figure, an asterisk (*) indicates that the p value is less than 0.05 at 0 weeks.

<Result>
As a result of the measurement of exhaled gas metabolism, (A) In the propolis intake + walking group, oxygen intake per body weight (Fig. 1), exercise intensity (Fig. 2), energy consumption (Fig. 5) were significant 4 weeks after the start of the test. There was a drop in numbers. (C) In the walking group, these values tended to increase, and no significant decrease in the values was observed. (B) In the propolis intake group, these values were significantly decreased from 4 weeks to 8 weeks later. In addition, the minute ventilation (FIG. 3) and the maximum expiratory rate (FIG. 4) were also significantly decreased in the (B) propolis intake group.

  In the questionnaire results, (B) fatigue in exercise (FIG. 6) and fatigue in daily life (FIG. 7) were significantly improved in the propolis intake group.

  From the above results, it was shown that propolis intake has an effect of improving exercise function even when the amount of exercise is insufficient, reducing the relative load on the same amount of exercise, and reducing fatigue. In addition, even when propolis intake and walking exercise were used in combination, it was shown that there was an effect of improving motor function and reducing fatigue, but these effects were better in the case of taking propolis without walking exercise. It was shown to be expensive.

Claims (3)

  An anti-fatigue agent comprising a bee-derived propolis belonging to the genus Bee or a processed product thereof as an active ingredient.   The fatigue reducing agent according to claim 1, which is for oral use.   A food composition for reducing fatigue, comprising the fatigue reducing agent according to claim 1.

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