JPWO2016136377A1 - Automatic analyzer - Google Patents

Abstract

When stirring the mixed solution of the sample and the reagent discharged from the sample, bubbles may be caught in the mixed solution. In the automatic analyzer that measures the light obtained by irradiating the liquid with light from the light source and obtains the amount of components in the liquid, the bubbles generated in this way refract, reflect, and scatter the light, May affect measurement results. In addition, if this bubble is ruptured or moved during the measurement, and there is a change in size due to a temperature change, this effect further increases. By providing a step on the side wall of the reaction vessel and guiding the bubbles generated during agitation to the top of the step, the light reflected by the bubbles does not enter the light receiving part, so the components in the liquid are not affected by the bubbles. Provided is an automatic analyzer capable of obtaining a quantity.

Description

  The present invention relates to an automatic analyzer that automatically analyzes components such as blood.

  As a device for analyzing target components contained in biological samples such as blood, transmitted light and scattered light of single or multiple wavelengths obtained by irradiating the reaction solution mixed with the sample and reagent with light from the light source Automatic analyzers that measure the amount of light are widely used.

  Automatic analyzers include devices for biochemical analysis that perform quantitative and qualitative analysis of target components in biological samples in the fields of biochemical tests and hematology tests, and blood coagulation that measures the coagulation ability of blood samples. There are devices for analysis.

  Blood, which is a sample used in the latter automatic analyzer, flows while maintaining fluidity inside the blood vessel, but once it bleeds, clotting factors present in plasma and platelets are activated in a chain, Blood fibrinogen is converted into fibrin and deposited, leading to hemostasis. The blood coagulation ability includes an exogenous one that coagulates blood leaked out of the blood vessel and an intrinsic one that coagulates blood in the blood vessel. The measurement items related to the analysis for determining blood coagulation ability as the blood coagulation time include prothrombin time (PT) in the extrinsic blood coagulation reaction test, activated partial thromboplastin time (APTT) in the intrinsic blood coagulation reaction test, and fibrinogen amount ( Fbg) and the like. In these items, it is necessary to sufficiently agitate the mixed solution of the sample and the reagent in order to stably progress the blood coagulation reaction.

  Patent Document 1 relates to a technology for stirring a mixed solution of a sample and a reagent, and in Patent Document 1, a recess for regulating and holding the shape of a liquid in a stirring container, and a side wall and a bottom wall having a predetermined thickness for defining the recess And the liquid held by the liquid held in the recess is configured so that the rising of the meniscus formed by the liquid held in the recess is lower than the rising of the meniscus formed by the liquid held in the container. A technique for uniformly stirring the liquid is described.

  Further, in Patent Document 2, a sound wave is irradiated to a reaction container containing a mixed solution of a sample and a reagent in order to exclude bubbles near the liquid surface generated at the time of dispensing the sample and the reagent from the measurement light path. The technology to be described is described.

JP 2006-349582 A Japanese Patent Laid-Open No. 2002-200725

  When the reagent is discharged to the sample, or when the mixed liquid of the sample and the reagent is stirred, bubbles may be generated and may be caught in the mixed liquid. In such a state, when an automatic analyzer is used to irradiate the liquid mixture with light from the light source and measure the amount of light such as transmitted light or scattered light, the bubbles are refracted by the detected light. As a result, measurement results may be affected by causing reflection, scattering, and the like. In addition, if the generated bubbles are repelled or moved during measurement, or if the volume changes due to temperature changes, the amount of transmitted or scattered light detected also fluctuates, resulting in highly accurate measurement. The result may not be obtained.

  In recent years, especially in automated analyzers, high-precision analysis is required, and since the amount of samples and reagents has been reduced, this effect can be said to be even greater.

  However, as described above, the configuration of the stirring container described in Patent Document 1 contributes to uniform stirring of the contained liquid. However, there is nothing regarding the influence of bubbles generated by such discharge and stirring operations. Not considered.

  Further, in Patent Document 2, bubbles generated during stirring can be removed by irradiating a sound wave to a reaction container containing a mixed solution of a sample and a reagent. It is necessary to provide a mechanism, and the apparatus configuration is complicated. Furthermore, in the blood coagulation analysis described above, the coagulation reaction may be started immediately after mixing the sample and the reagent, and it may not be possible to secure time for removing bubbles from the mixed solution.

  In the present invention, in view of the above-described problems, the influence on the measurement result due to the bubbles present in the mixed liquid of the sample and the reagent is reduced without requiring a complicated configuration, and high accuracy can be obtained even for a very small amount of measurement object. It is an object of the present invention to provide an apparatus for realizing analysis and a method using the apparatus.

  As one aspect for solving the above-described problems, a reaction container that contains a mixed liquid of a sample and a reagent, a dispensing mechanism that dispenses the sample and the reagent, and the mixed liquid are accommodated in the reaction container. A light source that emits light from the lower side of the reaction container, a light receiving unit that receives light emitted from the light source, an analysis unit, and a control unit that controls the operation of the dispensing mechanism, The inner side surface of the reaction vessel is provided with a step portion formed so that the diameter decreases toward the center, and the control unit dispenses the sample after dispensing the sample into the reaction vessel. With the mechanism in contact with the inner side surface of the reaction vessel, the reagent was dispensed into the reaction vessel containing the sample, and the total amount of the mixture of the sample and reagent was contained in the reaction vessel. In this state, the height of the liquid surface of the mixed solution is located above the stepped portion. Sea urchin, and controlling the operation of the dispensing mechanism

  According to the above-described aspect, the influence on the measurement result due to the bubbles present in the mixed solution of the sample and the reagent is reduced without requiring a complicated configuration, and high-precision analysis can be performed even for a small amount of measurement target. Can be realized.

The figure which shows the basic composition of the automatic analyzer which concerns on this Embodiment. The figure which shows the shape of the reaction container which concerns on this Embodiment (1st Embodiment). The figure which shows the structure of the reaction container and optical system which concern on this Embodiment (1st Embodiment). The enlarged view which shows the structure of the reaction container and optical system which concern on this Embodiment (2nd Embodiment). The enlarged view which shows the vicinity area | region of the level | step-difference part in the reaction container which concerns on this Embodiment (1st Embodiment). The flowchart which shows operation | movement of the reagent dispensing probe which concerns on this Embodiment (3rd Embodiment). The figure explaining operation | movement of the reagent dispensing probe which concerns on this Embodiment (3rd Embodiment). The figure explaining operation | movement of the reagent dispensing probe which dispenses the multiple types of solution which concerns on this Embodiment (4th Embodiment). The timing chart shown about the mixing of the sample and reagent which concern on this Embodiment (5th Embodiment), and data processing. The figure which shows the example of the other shape of the reaction container which concerns on this Embodiment (1st Embodiment). The figure explaining the angle which the side wall and step part of the reaction container concerning this Embodiment (2nd Embodiment) form.

  Hereinafter, embodiments for carrying out the present invention will be described in detail with reference to the drawings. Note that throughout the drawings, components having the same functions in the drawings are denoted by the same reference symbols in principle, and description thereof may be omitted.

(Configuration of the first embodiment)
<Overall configuration of device>
FIG. 1 shows a basic configuration of an automatic analyzer according to the present embodiment. Here, an example of an apparatus for performing blood coagulation analysis will be described as one aspect of the automatic analyzer. As shown in the figure, the automatic analyzer 100 mainly includes a sample disk 102, a reagent disk 104, a sample dispensing mechanism 106, a reagent dispensing mechanism 107, a sample dispensing port 108, an analysis port 109, and a reaction container supply unit 110. , A cleaning mechanism 111, a reaction container discarding unit 112, a reaction container transfer mechanism 113, a control unit 114, and the like.

  The sample disk 102 is a disk-shaped unit that can freely rotate in the left-right direction, and a plurality of sample containers (sample containers) 101 that contain samples such as standard samples and test samples can be arranged on the circumference thereof.

  Similar to the sample disk 102, the reagent disk 104 is a disk-shaped unit that is rotatable in the left-right direction, and includes a reagent container 103 that contains a reagent containing a component that reacts with a component of each inspection item included in the sample. A plurality can be arranged on the circumference. In addition, the reagent disk 104 can be configured so that the reagent in the arranged reagent container 103 can be kept cold.

  The reaction container transfer mechanism 113 transfers the reaction container 105 used for analysis from the reaction container supply unit 110 to the sample dispensing port 108. Further, the reaction container 105 into which the sample has been dispensed is transferred from the sample dispensing port 108 to the analysis port 109. After the analysis is completed, the reaction container 105 in the analysis port 109 is transferred to the reaction container discarding unit 112. The reaction container transfer mechanism 113 further includes a reciprocation between the reaction container discarding unit 112 and the sample dispensing port 108 as a reset operation before the start of analysis, and an operation after the completion of the analysis as the reaction container discarding unit 112 and the analysis port. A reciprocal motion with 109 is performed.

  The sample dispensing mechanism 106 sucks the sample in the sample container 101 held on the sample disk 102 and dispenses the sample into the reaction container 105 installed in the sample dispensing port 108. Here, the operation of the sample dispensing mechanism 106 is controlled based on an instruction of the control unit 114 in accordance with the operation of a sample syringe pump (not shown).

  The reagent dispensing mechanism 107 sucks the reagent in the reagent container 103 held on the reagent disk 104, and dispenses it into the reaction container 105 installed in the analysis port 109 and containing the sample. Here, the operation of the reagent dispensing mechanism 107 is controlled based on an instruction from the control unit 114 in accordance with an operation of a reagent syringe pump (not shown).

  In the cleaning mechanism 111, the sample dispensing mechanism 106 and the reagent dispensing mechanism 107 are cleaned.

  The light source 115 is installed below the reaction container 105, and the light receiving unit 116 is installed below the liquid level when the entire amount of the mixed liquid is stored in the reaction container 105. The installed reaction container 105 is irradiated with light from the light source 115 from below, and this light is scattered by the precipitate produced by the reaction of the mixed solution. As the amount of precipitates increases, the amount of scattered light also increases. Therefore, the amount of precipitates is calculated by detecting the scattered light with the light receiving unit 116. For example, in a coagulation test item, when a specimen and a reagent react, time passes and fibrin precipitates. As the fibrin is deposited, the amount of light scattered increases. By detecting this amount of light, the amount of fibrinogen (Fbg) can be inspected. In addition, by monitoring the amount of light in the same manner using a reagent corresponding to the test item, other coagulation items such as prothrombin time (PT) and activated partial thromboplastin time (APTT) can also be tested. For example, the light source 115 is disposed below the reaction vessel 105, and two light receiving units 116 are disposed facing each other so as to be 90 ° with respect to the optical axis of the light source 115. The amount of scattered light of the mixed solution irradiated from the lower part of the reaction vessel 115 increases as fibrin precipitates.

  In the above-described example, the configuration in which the two light receiving units 116 are provided on the side surface of the analysis port 109 with respect to one light source 115 has been described. However, the configuration is not limited thereto. Can be changed. In the case of a configuration including one light receiving unit 116, the light receiving unit 116 is not arranged facing the above-described arrangement, but is arranged at an appropriate angle such as 90 ° with respect to the optical axis of the light source 115.

The control unit 114 moves the sample disk 102, the reagent disk 104, the sample dispensing probe 106, and the reagent dispensing probe 107 vertically and horizontally, the operations of the sample syringe pump and reagent syringe pump (not shown), and the cleaning mechanism 111. Operation of various configurations constituting the automatic analyzer 100, such as the operation of supplying the cleaning water not to be performed, the operation of the light source 115 and the light receiving unit 116 of the analysis port 109, and the data processing operation such as the calculation of the concentration of the target component based on the detection result Perform control such as condition setting. In this figure, the control unit 114 is connected to each component unit and controls the entire automatic analyzer. However, each component unit may be provided with an independent control unit.
<Composition of reaction vessel>
Next, the configuration of the reaction vessel 105 according to the present embodiment will be described. FIG. 2 is a diagram showing the shape of the reaction vessel according to the present embodiment.

  For example, in a cylindrical cell having a diameter of about 6 mm and a total length of about 26 mm, the reaction vessel 105 is positioned below the inner diameter D1 on the upper side (opening 1503) side, with a position about 7 mm from the bottom as a boundary ( A stepped portion 1051 formed so that the inner diameter D2 on the side of the bottom 1504) becomes small (so that D1> D2 in the figure), and is positioned above the stepped portion 1051 and on the inner side wall surface of the cylindrical cell A protrusion 1052 that protrudes to form a convex shape is provided. For example, the height of the protrusion 1052 (horizontal width) is about 0.2 mm, and the width in the vertical direction with respect to the horizontal is about 1 mm. The distance from the opening 1053 to the protrusion 1052 is about 11 mm, and the distance from the bottom 1054 to the protrusion 1052 is about 15 mm. Regarding the operation of the sample dispensing mechanism 106 and the reagent dispensing mechanism 107, the control unit 114 sets the height of the liquid level S of the mixed solution in the reaction vessel 105 when the sample and the reagent mixed solution are all stored. The height of the stepped portion 1051 is higher than the height of the protruding portion 1052, and the amount of the protruding portion 1052 and the mixed liquid is not in contact with each other. Instruct and control to dispense the sample and the reagent into the reaction vessel 105, respectively. Here, the protrusions 1052 are formed at the same height along the inner side wall surface of the reaction vessel 105.

  For example, in the analysis using the reaction vessel 105 having the above-described dimensions, when the liquid volume of the mixed solution of the sample and the reagent is set to 75 μL, the height of the liquid surface S is about 8 mm from the bottom 1054. Even when the entire amount of the mixed liquid is accommodated, the height of the liquid surface S is located higher than the height of the stepped portion 1051, and the stepped portion 1051 is completely present in the liquid. Further, the height of the liquid level S is positioned below the protrusion 1052.

  In the reaction vessel 105 described above, the outer diameter of the cylindrical cell is made constant, and the lower (bottom portion 1054) is lower than the inner diameter D1 on the upper side (opening 1053 side) with the position where the stepped portion 1051 is formed as a boundary. The example in which the inner diameter D2 of the side) is reduced has been described. However, as shown in FIG. 10, for example, with the position where the step 1051 is formed as a boundary, the lower outer diameter is smaller than the upper outer diameter, that is, on the outer side wall of the reaction vessel 105. Alternatively, a step can be formed.

  However, since the shape of the reaction vessel 105 becomes complicated, the thickness of the side wall of the reaction vessel 105 in the region below the position where the stepped portion 1051 is formed should be thicker than other regions. Is desirable. In this case, since the outer diameter of the reaction vessel 105 is constant, there is an advantage that the analysis can be started without changing the configuration of the apparatus such as the arrangement of the optical system.

Here, the height (horizontal width) h of the stepped portion 1051 will be described with reference to FIG. FIG. 5 is an enlarged view of a region near the step portion 1051 in the reaction vessel 105 according to the present embodiment. Here, let r be the radius of the bubble 501 that can be generated in the mixed solution of the sample and the reagent. First, light emitted from the light source 115 enters the bubble 501 at an angle θ ′. Next, the light incident on the bubble 501 is reflected at an angle of θ. Since the light receiving unit 116 is located below the liquid level (not shown in the figure), in order to prevent the influence of the bubble 501 on the measurement result, the traveling direction of the reflected light is within the detection range of the light receiving unit 116. Must be configured not to be included.
In this figure, as an example, a case will be described in which the angle of the reflected light is set to be 90 ° or more with respect to the optical axis of the incident light so as not to be incident on the light receiving unit 116. In this case, when 2 (θ−θ ′) is 90 ° or more and, for example, θ ′ is 0, θ is set to 45 ° or more. Further, the height h of the step 1501 at this time is equal to or greater than r (1 + sin θ). For example, since the diameter of the bubble 501 that is likely to be generated is about 0.4 mm, if the height of the stepped portion 1051 is about 0.3 mm, the light reflected by the bubble 501 enters the light receiving unit 116. It will not be. Further, since the diameter of the bubble 501 that can be generated is large and is known to be about 1.0 mm, it is desirable that the step portion 1051h be configured to be in the range of about 0.2 mm to 1.0 mm. .

  FIG. 3 is a diagram showing the configuration of the reaction vessel 105 according to the present embodiment, and the optical system including the light source 115 and the light receiving unit 116. FIG. 3A shows the reaction vessel 105 that does not include the step 1501 but includes only the protrusion 1502. In this configuration, since the step portion 1501 is not provided, the light reflected after entering the bubble 501 is detected by the light receiving portion 116.

  On the other hand, FIG. 3B shows the reaction vessel 105 having both the stepped portion 1501 and the protruding portion 1502. In this configuration, since the step portion 1501 is provided, the light reflected after entering the bubble 501 is reflected at an angle larger than 90 ° with respect to the optical axis of the incident light. 116 is not detected.

  Here, in the present embodiment, the case where the angle of the reflected light is configured to be 90 ° or more with respect to the optical axis of the incident light is not limited to this, and as described later, Even if this angle is 90 ° or less, it may be configured so as to satisfy a condition that deviates from the detection range of the light receiving unit 116.

  In addition, here, the relationship with the detection range of the light receiving unit 116 based only on the presence or absence of the stepped portion 1501 has been described. It can also be set not to be included.

(Second Embodiment)
In this embodiment, the influence on the measurement due to the configuration of the step portion 1051 of the reaction vessel 105 described above will be described in more detail with reference to FIG. FIG. 4 is an enlarged view showing the configuration of the reaction vessel and the optical system according to the present embodiment. 4A shows the reaction vessel 105 without the stepped portion 1501, and FIG. 4B shows the reaction vessel 105 with the stepped portion 1501.

  As shown in FIG. 4B, the control unit 114 is configured such that the liquid level S of the mixed liquid is higher than the position of the stepped part 1501 in a state where the whole amount of the mixed liquid of the sample and the reagent is dispensed into the reaction container 105. The dispensing amount by the sample dispensing mechanism 106 and the reagent dispensing mechanism 107 is controlled so that the distance becomes upward. Here, when the same amount and the same dispensing conditions are used to dispense into each reaction vessel 105 in FIGS. 4 (a) and 4 (b) and contain the mixed solution, the liquid level S shown in FIG. 4 (b). Is a position higher than the height of the liquid surface S shown in FIG. Since the bubbles 501 generated in the mixed liquid float on the liquid surface S, the higher the liquid surface S is, the higher the position of the bubbles 501 is with respect to the light receiving unit 116. Accordingly, in FIG. 4B, when the light emitted from the light source 115 is incident on the bubble 501 and then reflected, it can be prevented from being included in the detection range of the light receiving unit 116. In other words, the reaction container 105 having the stepped portion 1501 can be configured such that the reflected light is not received without narrowing the viewing angle that is the detection range of the light receiving unit 116. Therefore, even when a larger bubble 501 is generated, the influence on the measurement result can be reduced. For example, when the reaction vessel 105 having the above dimensions is used, when the stepped portion 1051 is not provided as shown in FIG. 4 (a), when 75 μL of the mixed liquid of the sample and the reagent is contained as described above, The height of the liquid level S is about 5.2 mm from the bottom 1054. On the other hand, in the reaction vessel 105 provided with the step portion 1051 as shown in FIG. 4B, the height of the liquid level S is about 7.5 mm from the bottom portion 1054. Even if a large bubble 501 is generated, the reflected light generated by entering the bubble 501 does not enter the viewing angle of the light receiving unit 116, so that the influence on the measurement result of the bubble 501 can be excluded.

  Here, FIG. 11 is a diagram illustrating an angle at which the stepped portion 1051 is formed with respect to the side wall of the reaction vessel 105 according to the present embodiment. As shown in this figure, when the angle α at which the stepped portion 1501 is formed is 120 ° or more with respect to the side wall, the liquid can be stirred without scattering when the reagent is discharged. Further, when the angle α is larger than 150 °, the traveling direction of the light reflected by the bubble 501 falls within the range of the field of view of the light receiving unit 116. That is, when dispensing the reagent into the sample, the effect is that the optical path of the light reflected from the bubble 501 deviates from the visual field of the light receiving unit 116 at the time of analysis without mixing the bubble 501 in the mixed solution. In order to realize simultaneously, it is desirable to form the level | step-difference part 1051 so that the angle (alpha) which the side wall of the reaction container 105 and the level | step-difference part 1051 may be 120 degrees or more and 150 degrees or less.

  By providing the stepped portion 1051 on the side wall of the reaction vessel 105 in this way, the light reflected by the bubble 501 does not enter the detection range of the light receiving unit 116, and therefore measurement by the bubble 501 generated in the mixed liquid of the sample and the reagent. The influence of data disturbance and the like can be suppressed. In addition, when the reagent is dispensed, the control unit 114 agitates the sample over a wider area by controlling the operation so that the reagent dispensing mechanism 107 discharges the reagent at a position above the stepped portion 1051. Therefore, the effect of improving the stirring efficiency can be expected.

  The reaction vessel 105 is preferably made of a material that transmits light from the light source 115, such as a colorless and transparent plastic, and more preferably polypropylene. In addition, the same effect can be obtained by using glass or the like.

(Third embodiment)
In the present embodiment, operations of sample dispensing and reagent dispensing in the automatic analyzer will be described with reference to FIGS. Note that the execution contents of these flowcharts are controlled by the control unit 114. First, in step 601, the sample dispensing mechanism 106 sucks the sample 702 held in the sample container (sample container) 101 installed on the sample disk 102. The reaction container transfer mechanism 113 installs the reaction container 103 in the sample dispensing port 108. The sample dispensing mechanism 106 dispenses the sample 702 into the reaction container 105 installed in the sample dispensing port 108 (S601). The reaction container transfer mechanism 113 transfers the reaction container 105 from the sample dispensing port 108 to the analysis port 109 and installs it. The sample placed in the analysis port 109 is kept at a temperature suitable for the reaction by a constant temperature mechanism (not shown). For example, in the case of analysis of blood coagulation reaction, the temperature is kept at 37 ° C.

  In step 602, the reagent held in the reagent container 103 installed on the reagent disk 104 is aspirated by the reagent dispensing mechanism 107 (S602). The reagent dispensing probe 107 is equipped with a heater, and is kept at 37 ° C., for example, in the analysis of blood coagulation reaction. The aspirated reagent 703 is preheated in the reagent dispensing probe 107.

  After the preheating is completed, in step 603, the reagent dispensing mechanism 107 moves horizontally and moves to the center and upward of the reaction vessel 105 (S603). This is shown in FIG. The reagent dispensing probe 107a includes water 701 for extruding the reagent 703 and a gap 705 provided to prevent the reagent 703 from being diluted by the water 701 before discharging the reagent 703. Yes.

  In step 604, the reagent dispensing probe 107a of the reagent dispensing mechanism 107 is lowered (S604). At this time, the control unit 114 determines the liquid level when the height of the tip of the reagent dispensing probe 107a is below the projection 1052 and the mixed liquid 704 of the sample and the reagent is contained in its entirety. The lowering operation of the reagent dispensing probe 107a is controlled so as to be positioned within the range above S.

  Subsequently, in Step 605, the reagent dispensing probe 107a of the reagent dispensing mechanism 107 moves in the horizontal direction, the tip of the reagent dispensing probe 107a is in contact with the inner wall surface of the reaction vessel 105, and the reagent dispensing probe 107a is moved in the horizontal direction. The probe 107a stops at a position where the side surface of the probe 107a contacts the protrusion 1052 provided on the inner wall surface of the reaction vessel 105 (S605).

  In step 606, the reagent 703 is discharged in a state where the reagent dispensing probe 107a of the reagent dispensing mechanism 107 is stopped, that is, in a state where it is in contact with the protrusion 1052 (S606). Here, the sample 702 and the reagent 703 are mixed by the momentum when the reagent 703 is discharged. This state is shown in FIG.

  As shown in FIG. 7B, when discharging the reagent 703, the tip of the reagent dispensing probe 107a is brought into contact with a protrusion 1052 formed on the side surface on the inner side of the reaction container 105, whereby the reagent 703 is discharged. Flows along the inner wall to the lower side of the reaction vessel 105, and can be mixed with the sample 702 without entraining the bubbles 501. In addition, the sample 702 and the reagent 703 can be mixed more efficiently than when the reagent 703 is discharged from the vicinity of the center of the reaction vessel 105 and mixed. Note that in this embodiment, the liquid mixture of the sample 702 and the reagent 703 is stirred by the force with which the reagent 703 is discharged without stirring using a separate stirring unit.

  In step 607, after the reagent 703 is discharged, the reagent dispensing probe 107a of the reagent dispensing mechanism 107 is moved so as to return to the vicinity of the center of the reaction container (S607). This situation is shown in FIG.

  As shown in FIG. 7C, after the position of the reagent dispensing probe 107a is returned to the vicinity of the center of the reaction vessel 105, an ascending operation is performed in step 608 (S608). As another form, without passing through step 607, in step 608, the reagent dispensing probe 107a is moved up while moving the tip to the vicinity of the center of the reaction vessel 105, that is, moved up in an oblique direction. Can do. As described above, the reagent dispensing probe 107a can be raised after being moved close to the central portion of the reaction vessel 105, or can be moved obliquely upward.

  In step 609, the reagent dispensing probe 107a moves to the cleaning mechanism 111 in the horizontal direction after the ascending operation, and is cleaned (S609). This state is shown in FIG. In the cleaning mechanism 111, the reagent dispensing probe 107a is cleaned with the cleaning water 706.

  In step 610, if the next dispensing operation is necessary, the process returns to step 601 and the same operation is repeated. On the other hand, when the next dispensing operation is unnecessary, the process ends here.

(Fourth embodiment)
In the present embodiment, the dispensing operation of reagent dispensing in the automatic analyzer will be described with reference to FIG. 8, particularly the operation of dispensing a plurality of types of solutions using a common reagent dispensing mechanism.

  First, the reagent dispensing probe 107 a of the reagent dispensing mechanism 107 moves down from a predetermined position above the reaction container 105. At this time, the height of the tip of the reagent dispensing probe 107a is below the stepped portion 1051 and to a height within a range above the liquid level S when the entire amount of the first liquid 707 is stored. Descend.

  Subsequently, as shown in FIG. 8 (a), the reagent dispensing probe 107a moves in the horizontal direction and stops at a position where the tip of the reagent dispensing probe 107a is brought into contact with the stepped portion 1051 provided on the inner wall surface of the reaction vessel 105. To do.

  As shown in FIG. 8B, the first liquid 707 is discharged in a state where the reagent dispensing probe 107a is stopped, that is, in a state where the reagent dispensing probe 107a is in contact with the stepped portion 1051. After the reagent is discharged, the reagent dispensing probe 107a returns to the position near the center of the reaction vessel 105 and moves up as shown in FIG. After the ascending operation, the reagent dispensing probe 107a moves horizontally to the position where the cleaning mechanism 111 is provided, and is cleaned with the cleaning water 706 as shown in FIG. Here, the first liquid is designed so that the height of the liquid surface S is lower than the position of the step portion 1051 in a state where the entire amount of the first liquid is accommodated in the reaction vessel 105. By bringing the tip of the reagent dispensing probe 107a into contact with the step 1051 provided on the inner side wall of the reaction vessel 105, the range in which the first liquid is lifted by capillary action is within the range in contact with the step 1051. Therefore, the contamination range of the reagent dispensing probe 107a can be narrowed. Further, the first liquid does not adhere above the position of the step portion 1051.

  Subsequently, a second liquid (not shown) is sucked using the reagent dispensing probe 107 a and discharged into the reaction container 105. The subsequent operation is the same as the reagent dispensing operation shown in FIGS. When the reagent dispensing probe 107a ejects the second liquid, since the tip of the reagent dispensing probe 107a is located above the protrusion 1052, the first liquid adheres to the reagent dispensing probe 107a when the second liquid is ejected. There is nothing to do.

  For example, in the analysis item of blood coagulation time, when the first liquid and the second liquid are a sample and a reagent, the coagulation reaction starts when the two liquids are brought into contact with each other by the reagent dispensing probe 107a. As a result, the reactant is fixed to the reagent dispensing probe 107a, or the inside of the reagent dispensing probe 107a is clogged, and cleaning becomes difficult. Therefore, as described above, when the first liquid is discharged by the reagent dispensing probe 107a, the tip is brought into contact with the stepped portion 1051, and when the second liquid is discharged, the protrusion 1052 and the reagent dispensing probe 107a By bringing the side surface into contact with each other and bringing the tip into contact with the inner wall of the reaction vessel 105, it is possible to prevent the two kinds of liquids from being contaminated. In this case, the reagent dispensing mechanism 107 is used as a dispensing mechanism common to the sample dispensing mechanism 106.

(Fifth embodiment)
In this embodiment, the timing of sample and reagent mixing and data processing in the automatic analyzer will be described with reference to FIG. FIG. 9 is a timing chart showing mixing of a sample and a reagent and data processing according to the present embodiment.

  First, the time when the reagent 703 is discharged by the reagent dispensing mechanism 107 is set to a measurement time of 0 seconds. The sample 702 and the reagent 703 are mixed by the discharge force of the reagent 703. Moreover, stirring is advanced by the convection generated at this time. Gradually the convection decays and the convection settles after about 1 second. The bubble 501 is generated when the reagent 703 is discharged and contacts the sample 702. The bubble 501 moves in the mixed solution 704 along the convection of the sample 702 and the reagent 703. As the convection between the reagent 703 and the sample 702 attenuates, the bubbles 501 rise to the liquid surface S side. After about 1 second, when the convection is settled, the bubble 501 reaches the liquid surface S and stops. As the data processing operation, in the analysis port 109, the light receiving unit 116 starts to receive light from the light source 115 simultaneously with the discharge of the reagent 703. However, as described above, since the liquid mixture 704 is in convection for about 1 second, the photometric data contains a lot of noise. Data received after about 1 second after sample discharge, in which the convection between the reagent 703 and the sample 702 is settled, is used for data processing such as calculation of the concentration of the target component in the sample 702.

  As a modification of the above-described embodiment, the outer diameter of the reaction vessel 105 may be a polygon, such as a quadrangle, instead of a cylinder. Even in this case, an effect similar to that of the above-described embodiment can be obtained.

(Sixth embodiment)
In the above-described embodiment, the case where the reagent and the sample are stirred by the discharge force of the reagent has been described. However, the reagent and the sample may be configured to be stirred using a separate stirring mechanism. Here, examples of the stirring mechanism include a stirring bar, a spatula, and a method such as ultrasonic irradiation. Even in such a configuration, by making the configurations of the reaction vessel 105, the light source 115, and the light receiving unit 116 the same as the above-described embodiment, it is possible to obtain the effect of reducing the influence of the measurement result by the bubbles 501.

  In addition, this invention is not limited to above-described embodiment, Various modifications are included. For example, the above-described embodiment has been described in detail for easy understanding of the present invention, and is not necessarily limited to one having all the configurations described. Further, a part of the configuration of one embodiment can be replaced with the configuration of another embodiment, and the configuration of another embodiment can be added to the configuration of one embodiment. . Moreover, it is possible to add / delete / replace other configurations for a part of the configuration of each embodiment.

DESCRIPTION OF SYMBOLS 100 ... Automatic analyzer 101 ... Sample container 102 ... Sample disk 103 ... Reagent container 104 ... Reagent disk 105 ... Reaction container 106 ... Sample dispensing mechanism 107 ... Reagent Dispensing mechanism 107a ... Reagent dispensing probe 108 ... Sample dispensing port 109 ... Analysis port 110 ... Reaction vessel supply unit 111 ... Cleaning mechanism 112 ... Reaction vessel discarding unit 113 ... Reaction container transfer mechanism 114 ... control unit 115 ... light source 116 ... light receiving unit 501 ... bubble 701 ... water 702 ... sample 703 ... reagent 704 ... mixed solution 705 ··· Gaps 706 ··· Cleaning water 707 ··· First liquid 1051 ··· Step portion 1052 ··· Projection portion 1053 · · · Opening portion 1054 ··· Bottom

Claims (12)

A reaction vessel containing a mixture of the sample and the reagent;
A dispensing mechanism for dispensing the sample and the reagent into the reaction vessel;
An analysis unit comprising: a light source that emits light from the lower side of the reaction container in which the liquid mixture is housed; and a light receiving unit that receives light emitted from the light source;
A control unit for controlling the operation of the dispensing mechanism, and an automatic analyzer comprising:
On the inner side surface of the reaction vessel, provided with a stepped portion formed so that the diameter decreases toward the center,
The controller is
After dispensing the sample into the reaction vessel, with the dispensing mechanism in contact with the inner side surface of the reaction vessel, dispense the reagent into the reaction vessel containing the sample,
The operation of the dispensing mechanism so that the height of the liquid level of the mixed solution is located above the stepped portion in a state where the total amount of the mixed solution of the sample and the reagent is accommodated in the reaction container. An automatic analyzer characterized by controlling. An automatic analyzer according to claim 1, wherein
Furthermore, the side surface on the inner side of the reaction vessel is provided with a protruding portion that is located above the stepped portion and formed in a convex shape toward the central portion,
The control unit is configured so that the liquid level of the mixed solution is higher than the stepped portion in a state where the total amount of the mixed solution of the sample and the reagent is accommodated in the reaction vessel, and An automatic analyzer characterized in that the operation of the dispensing mechanism is controlled so as to be positioned below the protrusion. An automatic analyzer according to claim 1, wherein
The light receiving unit is
An automatic analyzer arranged below the stepped portion. An automatic analyzer according to claim 3, wherein
The controller is
An automatic analyzer that controls the light receiving range of the light receiving unit such that the light receiving range of the light receiving unit is below the liquid level of the liquid mixture. An automatic analyzer according to claim 2, wherein
The controller is
Operation of the dispensing mechanism such that the reagent is dispensed into the reaction container containing the sample in a state in which the dispensing mechanism is in contact with the protrusion formed on the inner side surface of the reaction container. An automatic analyzer characterized by controlling. An automatic analyzer according to claim 1, wherein
The automatic analysis apparatus characterized in that the reaction vessel has an inner diameter in a region above the boundary with the stepped portion as a boundary larger than a region in a region below the boundary. An automatic analyzer according to claim 1, wherein
The automatic analyzer is characterized in that the reaction vessel has a cylindrical shape. An automatic analyzer according to claim 1, wherein
The automatic analyzer is characterized in that the reaction vessel has a polygonal shape. An automatic analyzer according to claim 2, wherein
The controller is
In a state where the dispensing mechanism is in contact with the stepped portion, the sample is dispensed into the reaction container, and after the dispensing,
In a state where the dispensing mechanism is in contact with the protruding portion, the reagent is dispensed into a reaction container containing the sample.
An automatic analyzer that controls the operation of the dispensing mechanism. An automatic analyzer according to claim 1, wherein
The controller is
Analyzing the sample based on the output of the light receiving unit received after a lapse of a predetermined time after dispensing the reagent into a reaction container containing the sample by the dispensing mechanism. Automatic analyzer characterized by An automatic analyzer according to claim 10, wherein
The controller is
An automatic analyzer that starts receiving light from the light source by the light receiving unit together with dispensing of the reagent. An automatic analyzer according to claim 9, comprising:
The controller is
Range in which the position of the tip of the dispensing mechanism is below the protrusion and above the liquid level of the mixed solution in a state where the total amount of the mixed solution is accommodated in the reaction vessel An automatic analyzer characterized by controlling the operation of the dispensing mechanism so as to be located inside.

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