JP2014041144A - Automatic analyzer - Google Patents

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Publication number
JP2014041144A
JP2014041144A JP2013218645A JP2013218645A JP2014041144A JP 2014041144 A JP2014041144 A JP 2014041144A JP 2013218645 A JP2013218645 A JP 2013218645A JP 2013218645 A JP2013218645 A JP 2013218645A JP 2014041144 A JP2014041144 A JP 2014041144A
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Japan
Prior art keywords
reagent
sample
dispensing probe
dispensing
reagent dispensing
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JP2013218645A
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Japanese (ja)
Inventor
Takehiko Onuma
武彦 大沼
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Toshiba Corp
株式会社東芝
Toshiba Medical Systems Corp
東芝メディカルシステムズ株式会社
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Priority to JP2013218645A priority Critical patent/JP2014041144A/en
Publication of JP2014041144A publication Critical patent/JP2014041144A/en
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Abstract

An automatic analyzer capable of improving analysis data is provided.
A first reagent dispensing probe having an opening for sucking and discharging a first reagent at one end, and a first flow path W1 communicating with an opening inclined obliquely downward in one end including the one end. 14, a first reagent dispensing arm that movably holds the first reagent dispensing probe 14, and a mechanism for moving the first reagent dispensing arm up and down and rotating, the first reagent dispensing probe 14. Is stopped at the first reagent discharge position where the extension line 142 of the first flow path W1 intersects the inner surface other than the bottom surface in the reaction vessel 3.
[Selection] Figure 4

Description

The present invention relates to an automatic analyzer for analyzing a component of a test item contained in a liquid.
The present invention relates to an automatic analyzer equipped with a dispensing probe for dispensing a reagent used for analysis of a test item.

The automatic analyzer is intended for biochemical test items, immunological test items, etc., and changes in color tone and turbidity caused by the reaction of the mixture of the sample collected from the specimen and the reagent of each test item are optically measured by the photometry unit. By measuring, analytical data represented by the concentration of each test item component contained in the sample, the activity of the enzyme, and the like are generated.

In this automatic analyzer, a reagent container containing a reagent for each inspection item is stored in a reagent store.
Then, in order to analyze the inspection item to be inspected for each sample, the sample dispensing probe performs dispensing in which the sample accommodated in the sample container is sucked and discharged to the reaction container. In addition, the reagent dispensing probe performs dispensing by sucking the reagent in the reagent container and discharging it to the reaction container. Furthermore, after the stirrer stirs the mixed solution of the sample and the reagent dispensed in the reaction container, the photometric unit measures the stirred mixed liquid in the reaction container.

In reagent dispensing, a method is known in which a reagent dispensing probe sucks a reagent, stops at a discharge position, and discharges the reagent into a reaction container stopped below the discharge position (for example, Patent Documents). 1).

As in this Patent Document 1, in the separated discharge method in which the reagent is discharged away from the reaction container, if the discharge speed is slow, the reagent from the one end of the reagent dispensing probe that discharges the reagent deteriorates, and the reagent is discharged. A part of the reagent remains on the outer surface of the one end portion and the dispensing accuracy is lowered. For this reason, the reagent is discharged at a high speed.

JP 2008-145334 A

However, in the separated discharge method, as shown in FIG. 9A, when the reagent is discharged at a high speed into the reaction container into which the sample has been dispensed, the discharged reagent collides with the sample in the reaction container. There is a problem that the analysis data deteriorates due to scattering from a position where the sample is not involved in measurement higher than the height H when the mixed liquid in the reaction container is accommodated due to the momentum of collision or out of the reaction container 3.

In recent years, analysis has been performed using many types of reagents with the increase in analysis items that can be analyzed. These reagents are composed of a first reagent and a two-reagent first reagent and a second reagent that forms a pair with a two-reagent first reagent, and includes a reagent having physical properties that are easily bubbled. Then, when the first reagent that tends to foam is discharged into the reaction container into which the sample has been dispensed, a large amount of bubbles are generated in the upper layer of the mixture of the sample and the first reagent, as shown in FIG. 9B. Part of the sample is caught and held in the foam. Further, when the second reagent is discharged from above the bubble generated in the upper layer of the mixed solution of the sample and the first reagent, as shown in FIG. 9C, a part of the discharged second reagent is caught in the upper layer bubble. Held. As described above, when the sample and the second reagent are held in the bubbles, the sample and the second reagent held in the bubbles cannot be mixed with the lower mixed solution even if the sample and the second reagent are stirred. There is a problem that gets worse.

The present invention has been made in order to solve the above-described problems, and an object thereof is to provide an automatic analyzer capable of improving analysis data.

In order to achieve the above object, an automatic analyzer of the present invention dispenses a sample and a reagent into a reaction container, and in the automatic analyzer that measures the mixed solution, the reagent is sucked into the reaction container. A reagent dispensing probe having a discharge opening at one end and a flow path leading to the opening inclined obliquely downward in one end including the one end, and the sample dispensed to the reagent dispensing probe When entering the reaction vessel, among the inner surfaces other than the bottom surface in the reaction vessel,
The reagent is discharged from the reagent dispensing probe at a position where the reagent can be discharged toward the position of the liquid surface in the mixed solution of the reagent and the sample, and the reagent is removed from the stopped position by the reagent dispensing probe. And an analysis control means for discharging.

According to the present invention, a dispensing probe having an opening for sucking and discharging a reagent at one end and having a flow path leading to an opening inclined obliquely downward in one end including the one end is provided as an extension of the flow path. Analysis data can be improved by stopping at the discharge position where the line intersects the inner surface other than the bottom surface in the reaction vessel.

The block diagram which shows the structure of the automatic analyzer which concerns on the Example of this invention. The perspective view which shows the structure of the analysis part which concerns on the Example of this invention. Sectional drawing which shows the structure of the 1st reagent dispensing probe which concerns on the Example of this invention. The figure which shows the 1st reagent discharge position of the 1st reagent dispensing probe which concerns on the Example of this invention. The flowchart which shows the 1st reagent dispensing process based on the Example of this invention. The figure which shows each stop position of the 1st reagent dispensing probe in dispensing of the 1st reagent which concerns on the Example of this invention. The flowchart which shows the 2nd reagent dispensing process based on the Example of this invention. The figure which shows each stop position of the 2nd reagent dispensing probe in dispensing of the 2nd reagent which concerns on the Example of this invention. The figure which shows an example of the dispensing method of the reagent which concerns on background art.

  Examples of the present invention will be described below.

  Hereinafter, an embodiment of an automatic analyzer according to the present invention will be described with reference to FIGS.

FIG. 1 is a block diagram showing a configuration of an automatic analyzer according to an embodiment of the present invention. The automatic analyzer 100 measures a standard solution and test data by measuring a mixed solution of a sample such as a standard sample of each inspection item or a test sample collected from a specimen and a reagent used for analysis of each inspection item. An analysis unit 24 to be generated and an analysis control unit 25 that drives and controls each analysis unit related to the measurement of the analysis unit 24 are provided.

The automatic analyzer 100 also processes the standard data and test data generated by the analysis unit 24 to generate calibration data and analysis data, and the calibration data generated by the data processing unit 30. An output unit 40 that prints out and displays analysis data, an operation unit 50 that inputs various command signals, and the like, a system control unit that controls the analysis control unit 25, the data processing unit 30, and the output unit 40 in an integrated manner 60.

FIG. 2 is a perspective view showing the configuration of the analysis unit 24. The analysis unit 24 includes a sample container 17 that stores a sample such as a standard sample or a test sample, and a sample disk 5 that holds the sample container 17. In addition, a reagent container 6 containing a first reagent and a two-reagent system first reagent containing a component that reacts with a component of a test item included in the sample, a reagent container 1 for storing the reagent container 6, and a reagent container 1 And a reagent rack 1a for rotatably holding the reagent container 6 stored therein. In addition, a reagent container 7 that houses a second reagent that forms a pair with the first reagent of the two-reagent system, a reagent container 2 that stores the reagent container 7, and a reagent container 7 that is stored in the reagent container 2 can be rotated. Reagent rack 2a to hold
And. In addition, a plurality of reaction vessels 3 arranged on the circumference and a reaction disk 4 for rotatably holding the reaction vessel 3 are provided.

Further, the sample dispensing probe 16 that performs dispensing to suck the sample in the sample container 17 held on the sample disk 5 and discharge it into the reaction container 3, and the sample is sucked and discharged to the sample dispensing probe 16. A sample dispensing pump 16a and a sample dispensing arm 10 that holds the sample dispensing probe 16 in a movable manner. Further, a sample detector 16b that detects the liquid level of the sample in the sample container 17 held on the sample disk 5 by contact between the liquid level and the sample dispensing probe 16, and a washing tank that cleans the sample dispensing probe 16. 70.

In addition, a first reagent dispensing probe 14 for aspirating the first reagent in the reagent container 6 held in the reagent rack 1a and dispensing it into the reaction container 3 into which the sample has been dispensed, and the first reagent A first reagent dispensing pump 14a that causes the dispensing probe 14 to aspirate and discharge the first reagent, and a first reagent dispensing arm 8 that holds the first reagent dispensing probe 14 movably are provided. In addition, a first reagent detector 14b that detects the liquid level of the first reagent in the reagent container 6 held in the reagent rack 1a by contacting the liquid level with the first reagent dispensing probe 14, and a first reagent amount A cleaning tank 80 for cleaning the injection probe 14 is provided.

FIG. 3 is a cross-sectional view showing the configuration of the first reagent dispensing probe 14. The first reagent dispensing probe 14 has a tubular shape, has an opening 141 for sucking and discharging the first reagent at one end, and the other end is held by the first reagent dispensing arm 8. And the 1st flow path W1 which leads to the opening 141 inclined diagonally downward is formed in the one end part including one end. In addition, inside other than one end,
A second flow path W2 that is continuous with the first flow path W1 is formed in the vertical direction. Then, an extension line 142 extending through the center of the first flow path W1 and extending through the center of the flow path is inclined at an angle θ (θ <90 °) with respect to the central axis 143 passing through the center of the second flow path W2. Yes.

Further, the other end communicates with the first reagent dispensing pump 14a through a tube, and the pressure due to the suction and discharge operations from the first reagent dispensing pump 14a into the first and second flow paths W1, W2 and the tube. A pressure transmission medium such as pure water is transmitted. The first reagent dispensing pump 14
With the a suction operation of a, the first reagent in the reagent container 6 held in the reagent rack 1a is aspirated.
Further, the aspirated first reagent is discharged by the discharge operation of the first reagent dispensing pump 14a.

The analysis unit 24 shown in FIG. 2 includes a first stirrer 18 that stirs the mixed solution of the sample and the first reagent dispensed in the reaction vessel 3, and a first stirrer that holds the first stirrer 18 movably. Arm 20 and first
And a washing tank 18a for washing the stirring bar 18.

Also, a second reagent dispensing probe 15 for aspirating the second reagent in the reagent container 7 held in the reagent rack 2a and dispensing it into the reaction container 3 into which the first reagent has been dispensed, A second reagent dispensing pump 15a that causes the two reagent dispensing probe 15 to suck and discharge the second reagent; and a second reagent dispensing arm 9 that holds the second reagent dispensing probe 15 movably. Yes. In addition, a second reagent detector 15b that detects the liquid level of the second reagent in the reagent container 7 held in the reagent rack 2a by contacting the liquid level with the second reagent dispensing probe 15, and a second reagent amount A cleaning tank 90 for cleaning the injection probe 15 is provided.

In addition, a second solution for stirring the mixed solution of the sample, the first reagent, and the second reagent dispensed in the reaction container 3 is used.
A stirrer 19, a second stirrer arm 21 that holds the second stirrer 19 so as to be rotatable and vertically movable, and a cleaning tank 19 a that cleans the second stirrer 19 are provided. Further, the photometric unit 13 that optically measures the liquid mixture in the reaction vessel 3 by irradiating light, and the reaction vessel 3 that has finished the measurement by the photometric unit 13
And a reaction vessel cleaning unit 12 for cleaning the inside.

Then, the photometry unit 13 irradiates light to the reaction container 3 that rotates and crosses the optical path, and by this irradiation, the mixed solution of the sample and the first reagent in the reaction container 3, the sample, the first reagent, and the second Light transmitted through the reagent mixture is detected for each wavelength of the inspection item. Then, the detected detection signal is processed to generate standard data or test data represented by a digital signal, and the generated standard data or test data is output to the data processing unit 30.

The analysis control unit 25 includes a mechanism unit 26 having a mechanism for driving each analysis unit of the analysis unit 24 and a control unit 27 for controlling each mechanism of the mechanism unit 26. The mechanism unit 26 includes a mechanism for rotating the sample disk 5, the reagent rack 1 a, and the reagent rack 2 a, and a mechanism for rotating the reaction disk 4. The sample dispensing arm 10, the first reagent dispensing arm 8, the second reagent dispensing arm 9, the first stirring arm 20, and the second stirring arm 21 are each provided with a mechanism for rotating and vertically moving. Further, a mechanism for aspirating and discharging the sample dispensing pump 16a, the first reagent dispensing pump 14a, and the second reagent dispensing pump 15a, and a mechanism for moving the reaction container cleaning unit 12 up and down are provided.

The control unit 27 includes the sample disk 5 of the mechanism unit 26, the reagent rack 1a, the reagent rack 2a,
Reaction disk 4, sample dispensing arm 10, first reagent dispensing arm 8, second reagent dispensing arm 9, sample dispensing pump 16a, first reagent dispensing pump 14a, second reagent dispensing pump 15a
And a control circuit for controlling a mechanism for driving each analysis unit such as the reaction vessel cleaning unit 12. And the mechanism of each 1st and 2nd reagent dispensing arm 8 and 9 is controlled, and each 1st and 2nd reagent dispensing probe 14 and 15 is moved.

The control circuit of the first reagent dispensing arm 8 in the control unit 27 includes a turning mechanism for turning the first reagent dispensing arm 8, and an up / down movement for moving the turning mechanism and the first reagent dispensing arm 8 in the vertical direction. Control the moving mechanism. And the 1st reagent dispensing probe 14 is moved to each upper stop position of the reagent storage 1, the reaction disk 4, and the washing tank 80 by the height of a top dead center with a rotation mechanism. Further, a downward movement drive pulse is supplied to the vertical movement mechanism to move it downward from each upper stop position, and to stop at various positions.

Here, in the aspiration in dispensing the first reagent, the first reagent dispensing probe 14 is moved downward from the upper stop position of the reagent container 1. Then, based on the detection signal from the first reagent detector 14b, the first reagent can be aspirated from the position where the liquid level of the first reagent in the reagent container 6 is detected by the first reagent detector 14b. Stop at the first reagent suction position moved downward by a predetermined distance.

In discharging the first reagent, the first reagent dispensing probe 14 is moved downward from the upper stop position of the reaction disk 4, and a part including one end of the first reagent dispensing probe 14 is separated from the reaction container 3. Then, after entering the reaction container 3, the first reagent dispensing probe 14 is stopped based on the amount of the sample to be discharged into the reaction container 3 and the amount of the first reagent set in advance for each inspection item. Change the stop position.

Then, as shown in FIG. 4, the extension line 142 of the first flow path W1 in the first reagent dispensing probe 14 intersects the inner surface other than the bottom surface in the reaction container 3, and the position of the intersecting inner surface is set in advance. The sample is stopped at the first reagent discharge position above the mixed solution at the level of the liquid level S of the mixed solution composed of the first reagent. The first reagent dispensing probe 14 stopped at the first reagent discharge position discharges the first reagent toward the inner surface of the reaction container 3, and the discharged first reagent is the liquid level S of the mixed liquid on the inner surface of the reaction container 3. After reaching the intersection point P of the extension line 142, it descends along the inner surface of the reaction vessel 3.

In addition, after stopping the first reagent dispensing probe 14 at a position below the first reagent discharge position and above the sample discharged into the reaction container 3, while discharging the first reagent, The first reagent dispensing probe 14 is moved up to the first reagent discharge position so that one end is located above the liquid level of the mixed solution of the sample in the reaction container 3 and the discharged first reagent. May be.

The control circuit of the second reagent dispensing arm 9 of the control unit 27 includes a turning mechanism for turning the second reagent dispensing arm 9, and an up / down movement for moving the turning mechanism and the second reagent dispensing arm 9 in the vertical direction. Control the moving mechanism. And the 2nd reagent dispensing probe 15 is moved to each upper stop position of the reagent storage 2, the reaction disk 4, and the washing tank 90 by the height of a top dead center with a rotation mechanism. In addition, a downward movement drive pulse is supplied and moved downward from each upper stop position by the vertical movement mechanism, and stopped at various positions.

Here, in the suction in dispensing the second reagent, the second reagent dispensing probe 15 is moved downward from the upper stop position of the reagent storage 2. Then, based on the detection signal from the second reagent detector 15b, the second reagent can be aspirated from the position where the liquid level of the second reagent in the reagent container 7 is detected by the second reagent detector 15b. Stop at the second reagent suction position moved downward by a predetermined distance.
Further, in discharging the second reagent, the reaction is stopped at the upper stop position of the reaction disk 4, and the second reagent is discharged into the reaction container 3 at the stopped position.

The data processing unit 30 shown in FIG. 1 processes the standard data and test data output from the photometry unit 13 of the analysis unit 24 to generate calibration data and analysis data for each inspection item,
A data storage unit 32 that stores standard data and analysis data generated by the calculation unit 31 is provided.

The calculation unit 31 generates calibration data representing the relationship between the standard value and the standard data from the standard data output from the photometry unit 13 and a standard value set in advance for the standard sample of the standard data. The data is output to the output unit 40 and stored in the data storage unit 32.

Further, the calibration data of the inspection item corresponding to the test data output from the photometry unit 13 is read from the data storage unit 32. Then, analysis data represented as a concentration value or an activity value is generated from the test data output from the photometry unit 13 using the read calibration data. The generated analysis data is output to the output unit 40 and stored in the data storage unit 32.

The data storage unit 32 includes a memory device such as a hard disk, and stores the calibration data output from the calculation unit 31 for each inspection item. Moreover, the analysis data of each inspection item output from the calculation unit 31 is stored for each test sample.

The output unit 40 includes a printing unit 41 that prints out calibration data and analysis data output from the calculation unit 31 of the data processing unit 30 and a display unit 42 that displays and outputs the calibration data. And the printing part 4
1 includes a printer or the like, and prints the calibration data and analysis data output from the calculation unit 31 on printer paper or the like according to a preset format.

The display unit 42 includes a monitor such as a CRT or a liquid crystal panel, and displays calibration data and analysis data output from the calculation unit 31. In addition, an analysis parameter setting screen for setting analysis parameters such as the amount of the sample discharged into the reaction container 3 to analyze each inspection item, the amount of the first reagent, and the amount of the second reagent, and this analysis The reagent information setting screen for setting the information of the reagent used for the analysis of the inspection item set on the parameter setting screen is displayed. In addition, an inspection for selecting and setting an inspection item to be inspected from identification information such as a name and ID for identifying the test sample for each test sample and an inspection item set on the analysis parameter setting screen Display the item setting screen.

The operation unit 50 includes input devices such as a keyboard, a mouse, a button, and a touch key panel, and includes analysis parameters for each inspection item, reagent information, identification information and inspection items for a test sample, and identification information and a test for each test sample. An input operation is performed to set a target inspection item.

The system control unit 60 includes a CPU and a storage circuit, and inputs analysis parameter information, reagent information, identification information for each test sample, test item information, and the like input by an operation from the operation unit 50. After the information is stored in the storage circuit, the analysis control unit 25, the data processing unit 30, and the output unit 40 are integrated to control the entire system based on the input information.

Hereinafter, the dispensing process of the first and second reagents in the analysis unit 24 will be described with reference to FIGS. FIG. 5 is a flowchart showing a first reagent dispensing step for dispensing the first reagent. FIG. 6 is a diagram showing each stop position of the first reagent dispensing probe 14 in dispensing the first reagent. FIG. 7 is a flowchart showing a second reagent dispensing process for dispensing the second reagent. FIG. 8 is a diagram showing each stop position of the second reagent dispensing probe 15 in dispensing the second reagent.

First, the first reagent dispensing process for dispensing the first reagent will be described.
FIG. 5 is a flowchart showing the first reagent dispensing step. This first reagent dispensing step S1
At 0, the first reagent dispensing probe 14 moves from the upper stop position of the cleaning tank 80, which is the home position, and stops at the upper stop position of the reagent storage 1 (step S11).

After stopping at the upper stop position of the reagent storage 1, the first reagent dispensing probe 14 sucks a predetermined amount of air (step S12).

After the air is aspirated, the first reagent dispensing probe 14 moves downward from the upper stop position of the reagent storage 1 and inside the reagent container 6 held in the reagent rack 1a as shown in FIG. 6 (a). The liquid level of the first reagent is stopped at the first reagent suction position moved downward by a predetermined distance from the position detected by the first reagent detector 14b (step S13).

After stopping at the first reagent aspirating position, the first reagent dispensing probe 14 receives a dummy first from the reagent container 6 based on the information on the amount of the first reagent set on the analysis parameter setting screen of the display unit 42. The reagent and the first reagent for analysis are aspirated (step S14).

Here, when the first reagent is sucked by flowing the pressure transmission medium by the suction operation of the first reagent dispensing pump 14a, the pressure transmission medium remaining on the inner surface of the first reagent dispensing probe 14 is mixed into the first reagent. In addition, there is a problem that the sucked first reagent is diluted by diffusion of the first reagent into the pressure transmission medium. In order to avoid this problem, a predetermined amount of air and a dummy first reagent not used for analysis are aspirated. Subsequent to the suction of the dummy first reagent, the first reagent for analysis used for the analysis of each analysis item is sucked.

After aspirating the first reagent, the first reagent dispensing probe 14 moves upward from the first reagent aspirating position and stops at the upper stop position of the reagent storage 1. Next, the reagent container 1 is moved from the upper stop position and stopped at the upper stop position of the reaction disk 4 as shown in FIG. 6B (step S15).

After stopping at the upper stop position of the reaction disk 4, the first reagent dispensing probe 14 is displayed on the display unit 42.
Based on the information on the amount of the sample and the amount of the first reagent set on the analysis parameter setting screen, a part including one end moves downward from the upper stop position as shown in FIG. It stops at the first reagent discharge position that is separated from the reaction container 3 and enters the reaction container 3 (step S16).

After stopping at the first reagent discharge position, as shown in FIG. 6 (c), the first reagent dispensing probe 14 moves to a first point for analysis toward the intersection P on the inner surface of the reaction container 3 into which the sample has been dispensed. The reagent is discharged (step S17).

Thus, by stopping the first reagent dispensing probe 14 at the first reagent discharge position,
The first reagent can be discharged toward the intersection P on the inner surface of the reaction container 3. As a result, the discharged first reagent descends along the liquid level S or lower of the mixed liquid on the inner surface of the reaction vessel 3, so that all of the discharged first reagent is used as the first reagent involved in the measurement. be able to. Moreover, it can prevent that the one end part of the 1st reagent dispensing probe 14 touches a liquid mixture, and is contaminated.

Further, the first reagent dispensed from the first reagent dispensing probe 14 toward the intersection P on the inner surface of the reaction vessel 3.
Since the reagent travels down the inner surface of the reaction vessel 3 to lower the impact when colliding with the sample in the reaction vessel 3, a position where a part of the sample does not participate in the measurement in the reaction vessel 3 and Scattering out of the reaction vessel 3 can be suppressed.

Further, the first reagent discharged from the first reagent dispensing probe 14 toward the intersection P is the reaction container 3.
By descending along the inner surface, it is possible to reduce the impact when the colliding with the first reagent that descends and is stored in the reaction container 3, so the foaming of the first reagent discharged into the reaction container 3 is reduced. Can be suppressed. Thereby, it becomes possible to prevent a part of the sample from being caught and held in the foam, and the sample and the first reagent can be uniformly stirred by the first stirring bar 18. In addition, it is possible to prevent the second reagent from being discharged from above the bubbles, and the sample, the first reagent, and the second reagent can be uniformly stirred by the second stirring bar 19.

After discharging the first reagent for analysis, the first reagent dispensing probe 14 moves upward from the first reagent discharge position and stops at the upper stop position of the reaction disk 4. Next, the reaction disk 4 moves from the upper stop position and stops at the upper stop position of the cleaning tank 80. After stopping at the upper stop position of the cleaning tank 80, the inner and outer surfaces of the first reagent dispensing probe 14 in contact with the first reagent are cleaned in the cleaning tank 80 (step S18).

After the cleaning is performed, the first reagent dispensing probe 14 stops at the home position in preparation for the next dispensing of the first reagent (step S19).

Next, the second reagent dispensing step for dispensing the second reagent will be described.
FIG. 7 is a flowchart showing a second reagent dispensing process for dispensing the second reagent. In the second reagent dispensing step S30, the second reagent dispensing probe 15 moves from the upper stop position of the washing tank 90, which is the home position, and stops at the upper stop position of the reagent storage 2 (step S31).
.

After stopping at the upper stop position of the reagent storage 2, the second reagent dispensing probe 15 sucks a predetermined amount of air (step S32).

After the air is aspirated, the second reagent dispensing probe 15 moves downward from the upper stop position of the reagent storage 2 and inside the reagent container 7 held in the reagent rack 2a as shown in FIG. 8 (a). The liquid level of the second reagent is stopped at the second reagent suction position moved downward by a predetermined distance from the position detected by the second reagent detector 15b (step S33).

After stopping at the second reagent suction position, the second reagent dispensing probe 15 sucks the dummy second reagent and the second reagent for analysis in the reagent container 7 (step S34).

After aspirating the second reagent, the second reagent dispensing probe 15 moves upward from the second reagent aspirating position and stops at the upper stop position of the reagent storage 2. Next, the reagent container 2 moves from the upper stop position, and stops at the upper stop position of the reaction disk 4 as shown in FIG. 8B (step S35).

After stopping at the upper stop position of the reaction disk 4, the second reagent dispensing probe 15 starts from one end of a channel formed in a straight line in the vertical direction with the upper stop position of the reaction disk 4 as the second reagent discharge position. Then, the second reagent for analysis is discharged into the reaction container 3 into which the sample and the first reagent have been dispensed (step S36).

After discharging the second reagent for analysis, the second reagent dispensing probe 15 moves from the upper stop position of the reaction disk 4 and stops at the upper stop position of the cleaning tank 90. After stopping at the upper stop position of the cleaning tank 90, the inner and outer surfaces of the second reagent dispensing probe 15 in contact with the second reagent are cleaned in the cleaning tank 90 (step S37).

After the cleaning is performed, the second reagent dispensing probe 15 stops at the home position in preparation for the next dispensing of the second reagent (step S38).

In addition, a first channel that is inclined obliquely downward is formed inside the one end portion for sucking and discharging the second reagent, and the second channel in the vertical direction that is continuous with the first channel inside the other end portion. The first formed
It replaces with the 2nd reagent dispensing probe comprised similarly to the reagent dispensing probe 14. FIG. And
Move the replaced second reagent dispensing probe in the same manner as the first reagent dispensing probe 14,
The extension line of the first flow path of the second reagent dispensing probe intersects the inner surface other than the bottom surface in the reaction container 3, and the position of the intersecting inner surface is discharged into the reaction container 3, the first reagent, and the first reagent You may implement so that it may stop at the 2nd reagent discharge position above the liquid mixture which becomes the height of the liquid level of the liquid mixture of 2 reagents.

According to the embodiment of the present invention described above, in the first reagent dispensing, the first reagent dispensing in which the first flow path W1 leading to the opening 141 inclined obliquely downward is formed in the one end including the one end. Probe 1
4 is stopped at the first reagent discharge position where a part including one end is separated from the reaction vessel 3 and enters the reaction vessel 3, so that the first reagent is directed to the intersection P on the inner surface of the reaction vessel 3. Can be discharged. As a result, the discharged first reagent descends along the liquid level S or lower of the mixed liquid on the inner surface of the reaction vessel 3, so that all of the discharged first reagent is used as the first reagent involved in the measurement. be able to. Moreover, it can prevent that the one end part of the 1st reagent dispensing probe 14 touches a liquid mixture, and is contaminated.

Further, the first reagent dispensed from the first reagent dispensing probe 14 toward the intersection P on the inner surface of the reaction vessel 3.
Since the reagent travels down the inner surface of the reaction vessel 3 to lower the impact when colliding with the sample in the reaction vessel 3, a position where a part of the sample does not participate in the measurement in the reaction vessel 3 and Scattering out of the reaction vessel 3 can be suppressed.

Further, the first reagent discharged from the first reagent dispensing probe 14 toward the intersection P is the reaction container 3.
By descending along the inner surface, it is possible to reduce the impact when the colliding with the first reagent that descends and is stored in the reaction container 3, so the foaming of the first reagent discharged into the reaction container 3 is reduced. Can be suppressed. Thereby, it becomes possible to prevent a part of the sample from being caught and held in the foam, and the sample and the first reagent can be uniformly stirred by the first stirring bar 18. In addition, it is possible to prevent the second reagent from being discharged from above the bubbles, and the sample, the first reagent, and the second reagent can be uniformly stirred by the second stirring bar 19.

  As described above, analysis data can be improved.

P intersection point W1 first flow path 3 reaction vessel 4 reaction disk 14 first reagent dispensing probe 142 extension line

Claims (4)

  1. In an automatic analyzer that dispenses a sample and a reagent into a reaction vessel and measures the mixture,
    A reagent dispensing probe having an opening for sucking the reagent and discharging it into the reaction container at one end, and a flow path leading to the opening inclined obliquely downward in one end including the one end;
    When the reagent dispensing probe enters the reaction container into which the sample has been dispensed, among the inner surface other than the bottom surface in the reaction container, the reagent discharged from the reagent dispensing probe and the sample And an analysis control means for stopping at a position where the reagent can be discharged toward the liquid level in the mixed liquid, and discharging the reagent from the stop position by the reagent dispensing probe. Analysis equipment.
  2. The automatic analyzer according to claim 1, wherein the one end at the stop position is separated from the reaction container.
  3. Setting means for setting the amount of the sample to be discharged into the reaction container and the amount of the reagent;
    3. The automatic analyzer according to claim 1, wherein the analysis control unit is configured to change the stop position based on the amount of the sample and the reagent set by the setting unit. 4. .
  4. The reagent is a first reagent in a two-reagent system and a second reagent paired with the first reagent,
    The reagent dispensing probe is a first reagent dispensing probe for dispensing the first reagent, or the first reagent dispensing probe.
    2. A first reagent dispensing probe for dispensing a reagent and a second reagent dispensing probe for dispensing the second reagent into the reaction container into which the first reagent has been dispensed. The automatic analyzer according to claim 3.
JP2013218645A 2013-10-21 2013-10-21 Automatic analyzer Pending JP2014041144A (en)

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JPH06317599A (en) * 1993-05-10 1994-11-15 Olympus Optical Co Ltd Dispenser
JPH0777483A (en) * 1993-09-08 1995-03-20 Sanyo Electric Co Ltd Supporting device for dispensing plate and automatic dispenser
JPH07239334A (en) * 1994-02-25 1995-09-12 Fuji Photo Film Co Ltd Liquid mixing method
JP2001242183A (en) * 2000-02-28 2001-09-07 Hitachi Ltd Apparatus for processing liquid sample
JP2007278706A (en) * 2006-04-03 2007-10-25 Moritex Corp Device and method for injecting liquid
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