JP6121743B2 - Automatic analyzer - Google Patents

Description

  Embodiments described herein relate generally to an automatic analyzer for analyzing components contained in a liquid such as a sample collected from a subject.

  The automatic analyzer is intended for biochemical test items and immunological test items, and optically detects changes in color and turbidity caused by the reaction of the mixture of the sample collected from the specimen and the reagent used for analysis of each test item. taking measurement. By this measurement, analytical data represented by the concentrations of various test item components in the sample, enzyme activities, and the like are generated.

  In this automatic analyzer, each inspection item selected for each sample is analyzed. The sample dispensing probe sucks the sample stored in the sample container and discharges it to the reaction container, and the reagent dispensing probe sucks the reagent in the reagent container and discharges it to the reaction container. Next, the photometric unit measures the mixed solution of the sample and the reagent discharged into the reaction container.

  By the way, in analysis of each test item, serum or plasma separated from blood is often used as a sample. This sample may contain insoluble matter such as fibrin generated from blood components. When clogging occurs in the dispensing probe that sucks the sample due to the insoluble matter, the dispensing accuracy of the sample is reduced. Cause.

  To solve this problem, the clogging of the sample dispensing probe is detected by providing a pressure sensor that detects the pressure between the syringe and the sample dispensing probe that causes the sample dispensing probe to suck and discharge the sample. Automatic analyzers that can do this are known. In this automatic analyzer, the pressure when the sample dispensing probe is sucking the sample is detected, and clogging is detected when the detected pressure is smaller than the threshold value.

JP-A-10-48220

  However, although the pressure value when the sample is sucked after detection of clogging increases, there is a problem that analysis data deteriorates because it is lower than the threshold value and is not detected as clogging.

  The embodiment has been made to solve the above problems, and an object thereof is to provide an automatic analyzer that can prevent deterioration of analysis data due to clogging of a dispensing probe.

  In order to achieve the above object, an automatic analyzer according to an embodiment of the present invention is an automatic analyzer that dispenses a sample and a reagent into a reaction container and measures a mixed solution thereof. A dispensing probe that performs a suction, a detector that detects a pressure in the dispensing probe when any one of the suction of the sample, the ejection of the sample, or the ejection of the cleaning liquid is performed, and the detector It is determined whether or not the dispensing probe is in a normal state based on a pressure when the detected one is being performed and a preset first threshold value, and the dispensing probe is in an abnormal state If the one is performed, the dispensing probe is normal based on the pressure when the one detected by the detector is performed and a preset second threshold value. State Characterized by comprising a determination unit that not.

The block diagram which shows the structure of the automatic analyzer which concerns on embodiment. The perspective view which shows the structure of the analysis part which concerns on embodiment. The figure which shows an example of a structure of the sample dispensing probe which concerns on embodiment, a dispensing unit, a pressure detector, and a dispensing monitor part. The figure which shows an example of the change of the pressure detected by a pressure detector, when the sample dispensing probe which concerns on embodiment is attracting | sucking a sample. The flowchart which shows operation | movement of the automatic analyzer which concerns on embodiment.

  Hereinafter, embodiments will be described with reference to the drawings.

  FIG. 1 is a block diagram illustrating a configuration of an automatic analyzer according to the embodiment. This automatic analyzer 100 dispenses each sample such as a standard sample or test sample for each test item and an analysis reagent for each test item, and measures the mixture to obtain standard data or test data. An analysis unit 26 is provided. In addition, the drive unit 27 that moves each analysis unit related to the analysis of the analysis unit 26, the analysis control unit 28 that controls the drive unit 27, the state when the sample is dispensed in the analysis unit 26, and the like are monitored. And a dispensing monitor unit 30 for detecting an abnormality.

  The automatic analyzer 100 also processes the standard data and test data generated by the analysis unit 26 to generate calibration data and analysis data, and calibration data generated by the data processing unit 40. The output unit 50 for printing and displaying analysis data, the operation unit 60 for inputting various command signals, the analysis control unit 28, the dispensing monitor unit 30, the data processing unit 40, and the output unit 50 are integrated. And a system control unit 70 for controlling.

  FIG. 2 is a perspective view showing the configuration of the analysis unit 26. The analysis unit 26 includes a sample container 17 that stores a standard sample and a test sample for each inspection item, and a sample container holder 5 that holds the sample container 17. In addition, among the reagents for analysis of each test item, for example, a reagent container 6 that accommodates a first reagent and a first reagent based reagent, a reagent rack 1a that holds the reagent container 6, and a reagent rack 1a. And a reagent storage 1 for keeping the reagent container 6 cool. In addition, a reagent container 7 that stores a second reagent that forms a pair with the first reagent of the two-reagent system, a reagent rack 2a that holds the reagent container 7, and a reagent warehouse that cools the reagent container 7 held in the reagent rack 2a 2 are provided. Moreover, the reaction disk 4 which hold | maintains the several reaction container 3 arrange | positioned on the periphery rotatably is provided.

  In addition, the sample in the sample container 17 held by the sample container holder 5 is aspirated and discharged into the reaction container 3 and the sample dispensing probe 16 for discharging the cleaning liquid, and the sample dispensing probe 16 receives the sample. And a dispensing unit 18 for performing suction, sample discharge, and cleaning liquid discharge. Further, a pressure detector 19 that detects the pressure of the flow path that communicates between the sample dispensing probe 16 and the dispensing unit 18, and a liquid that detects the sample in the sample container 17 held by the sample container holder 5. A surface detector 16a, a sample dispensing arm 10 that holds the sample dispensing probe 16 so as to be rotatable and vertically movable, and a cleaning tank 16b that cleans the sample dispensing probe 16 are provided.

  In addition, a first reagent dispensing probe 14 for aspirating the first reagent in the reagent container 6 held in the reagent rack 1a and dispensing it into the reaction container 3 from which the sample has been discharged, and the first reagent dispensing A first reagent dispensing arm 8 that holds the dispensing probe 14 so as to be rotatable and vertically movable, a washing tank 14 a that cleans the first reagent dispensing probe 14, a sample discharged into the reaction container 3, and a first reagent And a first stirrer 20 for stirring the mixed solution.

  The second reagent dispensing probe 15 for aspirating the second reagent in the reagent container 7 held in the reagent rack 2a and dispensing it into the reaction container 3 from which the first reagent has been discharged; A second reagent dispensing arm 9 that holds the reagent dispensing probe 15 so as to be rotatable and vertically movable, and a cleaning tank 15 a that cleans the second reagent dispensing probe 15 are provided. In addition, a second stirrer 21 that stirs the mixed solution of the sample, the first reagent, and the second reagent in the reaction vessel 3, a photometric unit 13 that optically measures the reaction vessel 3 by irradiating light, and photometry And a cleaning nozzle 12 for cleaning the inside of the reaction vessel 3 for which the measurement has been completed by the unit 13.

  Then, the measurement unit 13 irradiates the reaction container 3 with light, and based on a detection signal that detects light transmitted through the liquid mixture containing the standard sample and the test sample in the reaction container 3, for example, absorbance or absorbance is measured. Generate standard data and test data expressed by the amount of change. Then, the generated standard data and test data are output to the data processing unit 40.

  The drive unit 27 includes a mechanism that rotates the sample container holder 5, the reagent rack 1 a, and the reagent rack 2 a, and a mechanism that rotates the reaction disk 4. In addition, a mechanism for rotating and vertically moving the sample dispensing arm 10, the first reagent dispensing arm 8, and the second reagent dispensing arm 9 is provided. Moreover, the mechanism which moves the washing nozzle 12 up and down is provided. Further, a mechanism for sucking and discharging the dispensing unit 18 is provided.

  The analysis control unit 28 includes a control circuit that controls each mechanism of the drive unit 27, and the sample container holder 5, the reagent rack 1a, the reagent rack 2a, the reaction disk 4, the sample dispensing arm 10, and the first reagent of the analysis unit 26. The units such as the dispensing arm 8, the second reagent dispensing arm 9, the washing nozzle 12, and the dispensing unit 18 are operated.

  The dispensing monitor unit 30 includes a pressure detected by the pressure detector 19 when the sample dispensing probe 16 of the analyzing unit 26 performs sample aspiration, sample ejection, or cleaning liquid ejection, and a preset first number. If it is determined whether the sample dispensing probe 16 is in a normal state based on the threshold value of 1 and it is determined that the sample dispensing probe 16 is in an abnormal state due to clogging, the sample dispensing probe 16 The warning information is output to the system control unit 70.

  In addition, after the sample dispensing probe 16 performs the suction of the sample, the discharge of the sample, or the discharge of the cleaning liquid after determining that the state is abnormal, the pressure detected by the pressure detector 19 and the second threshold value are set. Based on this, it is determined whether or not the sample dispensing probe 16 is in a normal state. When it is determined that the sample dispensing probe 16 is in an abnormal state, the abnormality information of the sample dispensing probe 16 is output to the system control unit 70.

  The data processing unit 40 shown in FIG. 1 includes a calculation unit 41 that processes the standard data and test data output from the photometry unit 13 of the analysis unit 26 to generate calibration data and analysis data for each inspection item, And a data storage unit 42 for storing the standard data and analysis data generated by the unit 41.

  The calculation unit 41 generates calibration data representing the relationship between the standard value and the standard data for each inspection item from the standard data output from the photometry unit 13 and the standard value preset for the standard sample of the standard data. The generated calibration data is output to the output unit 50 and saved in the data storage unit 42.

  Further, calibration data of the inspection item corresponding to the test data output from the photometry unit 13 is read from the data storage unit 42. Then, analysis data represented by a concentration value or an activity value is generated from the test data output from the photometry unit 13 using the read calibration data. The generated analysis data is output to the output unit 50 and stored in the data storage unit 42.

  The data storage unit 42 includes a memory device such as a hard disk, and stores the calibration data output from the calculation unit 41 for each inspection item. In addition, the analysis data of each inspection item output from the calculation unit 41 is stored for each test sample.

  The output unit 50 includes a printing unit 51 that prints and outputs calibration data and analysis data output from the calculation unit 41 of the data processing unit 40 and a display unit 52 that displays and outputs the data. The printing unit 51 includes a printer or the like, and prints the calibration data and analysis data output from the calculation unit 41 on printer paper or the like according to a preset format.

  The display unit 52 includes a monitor such as a CRT or a liquid crystal panel, and displays calibration data and analysis data output from the calculation unit 41. In addition, an analysis parameter setting screen for setting analysis parameters such as the amount of sample and reagent to be discharged into the reaction container 3 for analysis of each inspection item is displayed. In addition, warning information and abnormality information of the sample dispensing probe 16 output from the analysis monitor unit 30 and supplied from the system control unit 70 are displayed.

  The operation unit 60 includes input devices such as a keyboard, a mouse, a button, and a touch key panel, and performs input for setting analysis parameters, reagent information, and the like for each test item.

  The system control unit 70 includes a CPU and a storage circuit, stores input information such as analysis parameters of each inspection item input by an operation from the operation unit 60 in the storage circuit, and then analyzes based on the input information. The control unit 28, the dispensing monitor unit 30, the data processing unit 40, and the output unit 50 are integrated to control the entire system. Further, warning information and abnormality information output from the analysis monitor unit 30 are displayed on the display unit 52 of the output unit 50.

  Next, referring to FIG. 2 to FIG. 4, the details of the configuration of the sample dispensing probe 16, the dispensing unit 18, the pressure detector 19, and the dispensing monitor unit 30 related to the dispensing of the sample of the analysis unit 26 are described. Explained.

  FIG. 3 is a diagram illustrating an example of the configuration of the sample dispensing probe 16, the dispensing unit 18, the pressure detector 19, and the dispensing monitor unit 30. The sample dispensing probe 16 has an opening for sucking and discharging a sample at one end, and the other end is connected to one end of a tube 22 communicating with the dispensing unit 18. And it moves by the drive part 27 which drives the sample dispensing arm 10. FIG.

  The sample dispensing probe 16 collects an upper layer sample containing plasma or serum located in the upper layer and a lower layer sample containing formed components such as red blood cells, white blood cells, and platelets located in the lower layer by centrifugation of whole blood. The upper layer sample and the lower layer sample corresponding to the sample amount set for analysis of each inspection item are sucked from the sample container 17. Then, a set amount of sample is discharged into the reaction container 3.

  The dispensing unit 18 includes a syringe 181 having one end connected to the other end of the tube 22, a plunger 182 that fits into an opening provided in the other end of the syringe 181, a sample dispensing probe 16, the tube 22, and Each of the syringes 181 is configured by a tank 183 that stores a liquid pressure transmission medium such as pure water filled therein. Further, the pump 184 supplies the pressure transmission medium stored in the tank 183 into the syringe 181, and the open / close valve 185 opens and closes a flow path communicating between the syringe 181 and the pump 184.

  In the sample dispensing, the drive unit 27 moves the plunger 182 in the direction of the arrow L1 in a state where the flow path between the syringe 181 and the pump 184 is closed by the open / close valve 185 that is driven and controlled by the analysis control unit 28. The sample dispensing probe 16 sucks the sample in the sample container 17 by driving to suction. In the discharge, the drive unit 27 drives the plunger 182 to discharge in the direction of the arrow L2, so that the sample dispensing probe 16 discharges the sucked sample into the reaction container 3.

  Moreover, in the washing | cleaning for every completion | finish of dispensing of each sample, in the state which the flow path between the syringe 181 and the pump 184 is open | released by the on-off valve 185, the drive part 27 carries out washing drive of the pump 184, and sample dispensing The probe 16 discharges the pressure transmission medium as the cleaning liquid in the tank 183 supplied from the pump 184 into the cleaning tank 16b.

  The pressure detector 19 includes a pressure sensor 191 that measures the pressure of the flow path in the tube 22 filled with the pressure transmission medium with reference to atmospheric pressure, an amplifier 192 that amplifies the signal of the pressure sensor 191, and an amplifier 192. And an A / D converter 193 for converting the signal amplified in step 1 into a digital signal. Then, by measuring the pressure in the tube 22, the pressure in the sample dispensing probe 16 when the sample is sucked, the sample is discharged, or the cleaning liquid is discharged is detected. Then, the detected pressure data is output to the dispensing monitor unit 30.

  The dispensing monitor unit 30 determines the state of the sample dispensing probe 16 and the collecting unit 31 that collects pressure data output from the pressure detector 19 that detects the pressure in the sample dispensing probe 16 of the analysis unit 26. And a threshold value storage unit 32 for storing first to third threshold values set in accordance with the type of sample to be dispensed and the suction amount of the sample or the discharge amount of the cleaning liquid. The determination unit 33 determines whether the sample dispensing probe 16 is in a normal state based on the pressure data collected by the collection unit 31 and the first to third threshold values stored in the threshold storage unit 32. It has.

  When the sample dispensing probe 16 is sucking the upper layer sample, whether the sample dispensing probe 16 is in a normal state based on the pressure detected by the pressure detector 19 and the first and second threshold values. Determine whether or not. Further, when the sample dispensing probe 16 is discharging the cleaning liquid, it is determined whether or not the sample dispensing probe 16 is in a normal state based on the pressure detected by the pressure detector 19 and the third threshold value. . Further, when the sample dispensing probe 16 discharges the cleaning liquid after dispensing the lower layer sample having a higher suction resistance than the upper layer sample, the pressure detected by the pressure detector 19 and the first and second threshold values are set. Based on this, it is determined whether or not the sample dispensing probe 16 is in a normal state.

  The collection unit 31 collects pressure data from the pressure detector 19 at a preset timing according to the amount of suction when the sample dispensing probe 16 is sucking the upper layer sample. Further, pressure data from the pressure detector 19 is collected at a timing set in advance according to the discharge amount when the sample dispensing probe 16 discharges the cleaning liquid that is performed after dispensing the upper layer sample. Further, the pressure data from the pressure detector 19 is collected at a timing set in advance according to the discharge amount when the sample dispensing probe 16 discharges the cleaning liquid performed after dispensing the lower layer sample.

  The threshold storage unit 32 stores a plurality of first threshold values set according to the suction amount of the upper layer sample sucked by the sample dispensing probe 16 based on the sample amount set for analysis of each inspection item. ing. In addition, a plurality of second threshold values set in accordance with the suction amount of the upper layer sample to be sucked after being determined to be in an abnormal state due to clogging based on the first threshold value are stored. In addition, a third threshold set according to the discharge amount of the cleaning liquid discharged after it is determined that the state is abnormal based on the first threshold is stored.

  The threshold storage unit 32 stores a first threshold that is set in advance according to the discharge amount of the cleaning liquid discharged first time after the sample dispensing probe 16 dispenses the lower layer sample. In addition, a second threshold value which is set in advance according to the discharge amount of the cleaning liquid discharged for the second time after it is determined that the state is abnormal based on the first threshold value of the cleaning liquid is stored.

  The first threshold value used for the determination when the upper layer sample is sucked is set for each sample suction amount, and includes the upper layer sample of serum or plasma that does not contain clogging insoluble matter and clogging insoluble matter. This is obtained by obtaining the relationship between the pressure when dispensing the upper layer sample and the dispensing accuracy from an experiment for each suction amount.

  The determination unit 33 is based on the first threshold value set according to the pressure detected by the pressure detector 19 and the suction amount of the upper layer sample when the prepared sample dispensing probe 16 is sucking the upper layer sample. Thus, it is determined whether or not the sample dispensing probe 16 is in a normal state. If it is determined that the state is abnormal, the warning information of the sample dispensing probe 16 is output to the system control unit 70. In order to remove the insoluble portion from the warped sample dispensing probe 16, a predetermined amount of cleaning liquid is discharged. Further, when it is determined that the state is normal, the determination is made based on the first threshold set in accordance with the suction amount when the upper layer sample after the determination is sucked.

  However, it is estimated that only a predetermined amount of the cleaning liquid after being determined to be in an abnormal state is caused by a part of the insoluble matter remaining in the sample dispensing probe 16. Note that there may be a problem that the sample of the set sample amount cannot be accurately dispensed. In preparation for this situation, the second threshold is used.

  The second threshold value used for the determination at the time of suction of the upper layer sample is set for each sample suction amount, and is determined to be in an abnormal state based on the first threshold value. After the cleaning liquid is discharged, the upper layer sample is sucked. The relationship between the pressure and the dispensing accuracy of the sample is obtained by experiments for each suction amount. Then, when the amount of sample suction when determining using the first and second threshold values is the same, the second threshold value is set so that the pressure value is larger than the first threshold value.

  In this way, by setting the second threshold value and making a determination based on the set second threshold value, it is possible to accurately detect the insoluble matter remaining in the sample dispensing probe 16 as a cause of clogging.

  Based on the determination of clogging based on the first threshold, the determination unit 33 determines whether the sample dispensing probe 16 is sucking the upper layer sample after ejecting the cleaning liquid, according to the pressure and the suction amount detected by the pressure detector 19. Whether or not the sample dispensing probe 16 is in a normal state is determined based on the second threshold value set in the above. And when it determines with it being in a normal state, it determines based on the 1st threshold value set according to the amount of suction at the time of the suction of the upper layer sample after determination. Further, when it is determined that the state is abnormal, it is determined that the insoluble portion cannot be removed from the sample dispensing probe 16, and the abnormality information of the sample dispensing probe 16 that stops the subsequent dispensing is indicated by the system control unit 70. Output to.

  The third threshold value used for determination at the time of cleaning after dispensing the upper layer sample is that a predetermined amount of cleaning liquid is discharged after it is determined that the sample dispensing probe 16 is in an abnormal state based on the first threshold value. It is obtained by calculating | requiring from the experiment the relationship between the pressure at the time of being discharged, and the dispensing precision of the sample dispensed after discharging a washing | cleaning liquid.

  The determination unit 33 is set according to the pressure detected by the pressure detector 19 and the discharge amount of the cleaning liquid when the sample dispensing probe 16 is discharging the cleaning liquid based on the determination of clogging based on the first threshold value. Based on the third threshold value, it is determined whether or not the normal state. If it is determined that the sample is in an abnormal state, it is determined that the insoluble portion cannot be removed from the sample dispensing probe 16, and the abnormal information of the sample dispensing probe 16 that stops the subsequent dispensing of the sample is controlled by the system. To the unit 70.

  The first threshold value used at the time of the first cleaning after the lower layer sample is dispensed is, for example, insoluble after the first cleaning liquid is discharged and the pressure when the first cleaning liquid is discharged after the lower layer sample is dispensed. It was obtained by obtaining the relationship of the dispensing accuracy when dispensing the upper layer sample not containing an object from the experiment. In addition, the second threshold value used in the second cleaning includes the pressure when the second cleaning liquid is discharged after dispensing the lower layer sample and the upper layer that does not contain insolubles after the second cleaning liquid is discharged. It was obtained by obtaining the relationship of dispensing accuracy when dispensing a sample from an experiment. Then, when the discharge amount of the cleaning liquid at the time of determination using the first and second threshold values is the same amount, the second threshold value is set so that the pressure value is smaller than the first threshold value.

  The determination unit 33 is set according to the pressure detected by the pressure detector 19 and the discharge amount of the cleaning liquid when the sample dispensing probe 16 is discharging the first cleaning liquid after dispensing the lower layer sample. Based on the threshold value of 1, it is determined whether or not the state is normal. And when it determines with it being in an abnormal state, the 2nd washing | cleaning liquid is discharged from the sample dispensing probe 16. FIG. Further, when it is determined that the sample is in a normal state, when the sample to be dispensed after the determination is an upper layer sample, the determination is made based on the first threshold set according to the suction amount at the time of suction. When the subsequent sample is a lower layer sample, the determination is made based on the first threshold value at the first cleaning after dispensing.

  Further, when the sample dispensing probe 16 is discharging the second cleaning liquid, the determination unit 33 is based on the second threshold value set according to the pressure detected by the pressure detector 19 and the discharge amount of the cleaning liquid. To determine whether it is normal. And when it determines with it being in a normal state, when the sample after determination is an upper layer sample, it determines based on the 1st threshold set according to the amount of suction at the time of aspiration, and the sample after determination When is a lower layer sample, it is determined based on the first threshold value at the first washing after dispensing. When it is determined that the sample is in an abnormal state, it is determined that the insoluble portion cannot be removed from the sample dispensing probe 16, and the abnormality information of the sample dispensing probe 16 that stops the subsequent dispensing of the sample is controlled by the system. To the unit 70.

  In the system control unit 70, based on the warning information of the sample dispensing probe 16 from the determination unit 33, the warning information of the sample dispensing probe 16 is output to the output unit 50 and the analysis control unit 28 causes the sample dispensing probe 16 to output the warning information. The sample dispensing is stopped, and the cleaning liquid is discharged to perform cleaning. Further, based on the abnormality information from the determination unit 33, the abnormality information of the sample dispensing probe 16 is output to the output unit 50, and the analysis control unit 28 stops the subsequent dispensing of the sample.

  The first threshold value and a pressure value smaller than the first threshold value when the first threshold value and the sample discharge amount are the same according to the discharge amount of the upper layer sample discharged by the sample dispensing probe 16. When the second threshold value is set and the sample dispensing probe 16 is discharging the upper layer sample, the determination unit 33 is set according to the pressure detected by the pressure detector 19 and the discharge amount of the upper layer sample. It is determined whether or not it is in a normal state based on the threshold value. And when it determines with it being in an abnormal state, in order to remove an insoluble part by the sample dispensing probe 16, a predetermined amount of washing | cleaning liquid is discharged. Further, when it is determined that the state is normal, the determination is made based on the first threshold value set according to the discharge amount when discharging the upper layer sample after the determination. Next, when the sample dispensing probe 16 is ejecting the upper layer sample after ejecting the cleaning liquid based on the abnormality determination based on the first threshold, the pressure detected by the pressure detector 19 and the ejection amount of the upper layer sample are determined. Based on the set second threshold value, it is determined whether or not the normal state. If it is determined that the state is abnormal, it is determined that the insoluble portion cannot be removed from the sample dispensing probe 16, and the subsequent dispensing of the sample is stopped. Further, when it is determined that the state is normal, the determination may be performed based on the first threshold value set according to the discharge amount when discharging the upper layer sample after the determination.

  FIG. 4 is a diagram showing an example of a change in pressure detected by the pressure detector 19 when the sample dispensing probe 16 is sucking a sample. This change in pressure is represented by three types of first to third curves W1 to W3 on the coordinates where the horizontal axis is a unit of time and the vertical axis is a unit of pressure. Then, first and second threshold values L1 and L2 corresponding to the suction amount of the upper layer sample sucked by the sample dispensing probe 16 are set. The second threshold value L2 is set to be a pressure value larger than the first threshold value L1 when it is the same as the sample suction amount when the first threshold value L1 is used.

  The first curve W1 shows a change in pressure when the prepared sample dispensing probe 16 is sucking a predetermined amount of an upper layer sample that does not contain an insoluble matter that causes clogging. As described above, although the pressure slightly decreases after the suction of the sample is started, the pressure data collected at the timing Tg indicates a pressure value larger than the first threshold value L1, and therefore the determination unit 33 determines the sample amount. It is determined that the probe 16 is in a normal state.

  The second curve W2 shows the change in pressure when the prepared sample dispensing probe 16 sucks the same amount of the upper layer sample containing the insoluble matter that causes clogging as in the case of the first curve W1. Yes. As described above, the pressure is much lower than that in the case of the first curve W1 after the suction of the sample is started, and the pressure data collected at the timing Tg shows a pressure value smaller than the first threshold value L1. Therefore, the determination unit 33 determines that the sample dispensing probe 16 is clogged and is in an abnormal state.

  The third curve W3 draws the same amount of the upper layer sample that does not contain insoluble matter as the first curve W1 after the sample dispensing probe 16 determined to be clogged discharges a predetermined amount of cleaning liquid. It shows the change in pressure when As described above, since the pressure is less decreased than that in the case of the second curve W2 after the suction of the sample is started, the sample is dispensed because the pressure is significantly lower than that in the case of the first curve W1. It is estimated that a part of the insoluble matter remains on the probe 16 and the suction resistance increases. Since the pressure data collected at the timing Tg indicates a pressure value larger than the first threshold value L1 and a pressure value smaller than the second threshold value L2, the determination unit 33 uses the sample dispensing probe 16. Therefore, it is determined that the clogging is lower than that in the case of the second curve W2 and the state is abnormal.

  As described above, when the sample is dispensed after the cleaning liquid is discharged from the sample dispensing probe 16 determined to be in an abnormal state using the first threshold L1, the second threshold is used. By determining using L2, the insoluble matter remaining as a cause of clogging of the sample dispensing probe 16 that is determined to be in the normal state with the first threshold value L1 is detected and determined to be in an abnormal state. be able to.

  Hereinafter, an example of the operation of the automatic analyzer 100 will be described with reference to FIGS. 1 to 5.

  FIG. 5 is a flowchart showing the operation of the automatic analyzer 100. On the analysis parameter setting screen of the display unit 52, the sample amount VA is set for the inspection item A, and the sample amount VB is set for the inspection item B. Then, the sample container 17 that stores the upper layer sample P1 that performs the analysis of the inspection item A and the sample container 17 that stores the upper layer sample P2 that performs the analysis of the inspection item B are held by the sample container holder 5. And if operation of measurement start is performed from the operation part 60, the automatic analyzer 100 will start operation | movement (step S1).

  The system control unit 70 instructs the analysis control unit 28, the dispensing monitor unit 30, the data processing unit 40, and the output unit 50 to perform analysis. The analysis control unit 28 controls the drive unit 27 to operate each analysis unit of the analysis unit 26. The sample dispensing probe 16 of the analysis unit 26 moves to a suction position where the upper layer sample P1 in the sample container 17 can be sucked. Next, the upper layer sample P1 for analyzing the inspection item A in the sample container 17 is sucked (step S2).

  When the sample dispensing probe 16 is sucking the upper layer sample P1, the collection unit 31 of the dispensing monitor unit 30 collects the pressure data output from the pressure detector 19 and outputs it to the determination unit 33 (step S3). ).

  The determination unit 33 reads from the threshold storage unit 32 the first threshold value set according to the sample type (upper layer sample) of the inspection item A and the suction amount of the sample amount VA. Then, based on the read first threshold and the pressure data collected by the collecting unit 31, it is determined whether or not the sample dispensing probe 16 is in a normal state.

  When the collected pressure data is a pressure value smaller than the first threshold value (No in step S4), it is determined that the sample dispensing probe 16 is in an abnormal state. If the collected pressure data is a pressure value equal to or higher than the first threshold value (Yes in step S4), it is determined that the sample dispensing probe 16 is in a normal state, and the process proceeds to step S10.

  After “No” in step S4, the determination unit 33 outputs warning information to the system control unit. Based on the warning information from the determination unit 33, the system control unit 70 causes the output unit 50 to output warning information of the sample dispensing probe 16, and causes the analysis control unit 28 to stop dispensing the upper layer sample P <b> 1. The probe 16 is cleaned.

  In this way, it is determined whether or not the sample dispensing probe 16 is in a normal state based on the first threshold value. If the sample dispensing probe 16 is in an abnormal state, the sample dispensing is stopped and cleaning is performed. Can do. Thereby, deterioration of analysis data can be prevented beforehand.

  After stopping dispensing of the upper layer sample P1, the sample dispensing probe 16 moves to the cleaning tank 16b, discharges the upper layer sample P1, and then discharges the cleaning liquid. Next, the sample moves to a suction position where the upper layer sample P2 in the sample container 17 can be sucked, and the upper layer sample P2 for analyzing the inspection item B is sucked (step S5).

  When the sample dispensing probe 16 is sucking the upper layer sample P2, the collection unit 31 collects pressure data output from the pressure detector 19 at a predetermined timing and outputs the pressure data to the determination unit 33 (step S6).

  The determination unit 33 reads out from the threshold storage unit 32 the second threshold value set according to the sample type (upper layer sample) of the inspection item B and the suction amount of the sample amount VB. Then, based on the read second threshold value and the pressure data collected by the collecting unit 31, it is determined whether or not the sample dispensing probe 16 is in a normal state.

  If the collected pressure data is a pressure value smaller than the second threshold value (No in step S7), it is determined that the sample dispensing probe 16 is in an abnormal state. If the collected pressure data is a pressure value equal to or greater than the second threshold value (Yes in step S7), it is determined that the sample dispensing probe 16 is in a normal state, and the process proceeds to step S17.

  After “No” in step S 7, the determination unit 33 outputs abnormality information to the system control unit 70. The system control unit 70 causes the analysis control unit 28 to stop dispensing the subsequent samples and causes the output unit 50 to output abnormality information of the sample dispensing probe 16. The sample dispensing probe 16 stops dispensing the upper layer sample P2 (step S8).

  The output unit 50 outputs abnormality information indicating that, for example, the sample dispensing probe 16 is clogged and subsequent dispensing is impossible (step S9).

  Thus, when it is determined that the state is abnormal based on the first threshold, the sample dispensing probe 16 is in a normal state based on the second threshold when dispensing the sample after discharging the cleaning liquid. It is determined whether or not the sample is dispensed. If the sample is in an abnormal state, the subsequent dispensing of the sample can be stopped. Thereby, deterioration of analysis data can be prevented beforehand.

  After “Yes” in Step S4, the sample dispensing probe 16 moves to the reaction container 3 and discharges the upper layer sample P1 (Step S10).

  The sample dispensing probe 16 that has finished dispensing the upper layer sample P1 moves to the washing tank 16b and discharges the washing liquid. After discharging the cleaning liquid, the sample dispensing probe 16 moves to a suction position where the upper layer sample P2 in the sample container 17 can be sucked, and sucks the upper layer sample P2 for analyzing the inspection item B (step S11).

  When the sample dispensing probe 16 is sucking the upper layer sample P2, the collection unit 31 collects pressure data output from the pressure detector 19 and outputs the pressure data to the determination unit 33 (step S12).

  The determination unit 33 reads from the threshold storage unit 32 the first threshold value set according to the sample type (upper layer sample) and the sample amount VB of the inspection item B. Then, based on the read first threshold and the pressure data collected by the collecting unit 31, it is determined whether or not the sample dispensing probe 16 is in a normal state.

  When the collected pressure data is a pressure value smaller than the first threshold value (No in step S13), it is determined that the sample dispensing probe 16 is in an abnormal state. If the collected pressure data is a pressure value equal to or higher than the first threshold value (Yes in step S13), it is determined that the sample dispensing probe 16 is in a normal state, and the process proceeds to step S17.

  After “No” in step S13, the determination unit 33 outputs warning information to the system control unit. The system control unit 70 causes the analysis control unit 28 to stop dispensing the upper layer sample P2 and to clean the sample dispensing probe 16. In addition, the warning information of the sample dispensing probe 16 is output to the output unit 50.

  In this way, it is determined whether or not the sample dispensing probe 16 is in a normal state based on the first threshold value. If the sample dispensing probe 16 is in an abnormal state, the sample dispensing is stopped and cleaning is performed. Can do. Thereby, deterioration of analysis data can be prevented beforehand.

  The sample dispensing probe 16 stops dispensing the upper layer sample P2. And it moves to the washing tank 16b and discharges a washing | cleaning liquid (step S14).

  The collection unit 31 collects pressure data output from the pressure detector 19 and outputs the pressure data to the determination unit 33 when the sample dispensing probe 16 is discharging the cleaning liquid (step S15).

  The determination unit 33 reads out the third threshold value set according to the discharge amount of the cleaning liquid from the threshold value storage unit 32. Then, based on the read third threshold value and the pressure data collected by the collecting unit 31, it is determined whether or not the sample dispensing probe 16 is in a normal state.

  If the collected pressure data is a pressure value larger than the third threshold value (No in step S16), it is determined that the sample dispensing probe 16 is in an abnormal state, and the process proceeds to step S9. If the collected pressure data is a pressure value equal to or lower than the third threshold value (Yes in step S16), it is determined that the sample dispensing probe 16 is in a normal state, and the process proceeds to step S19.

  Thus, when it is determined that the state is abnormal based on the first threshold value, whether or not the sample dispensing probe 16 is in a normal state based on the third threshold value at the time of cleaning after the determination is made. If it is determined that the state is abnormal, the subsequent dispensing of the sample can be stopped. Thereby, deterioration of analysis data can be prevented beforehand.

  After “Yes” in Step S7 or “Yes” in Step S13, the sample dispensing probe 16 moves to the reaction container 3 and discharges the upper layer sample P2 (Step S17).

  After step S17, the sample dispensing probe 16 moves to the cleaning tank 16b and discharges the cleaning liquid (step S18). Thereafter, the process proceeds to step S19.

  After step S9, “Yes” in step S16, or step S18, the system control unit 70 instructs the analysis control unit 28 to stop each analysis unit of the analysis unit 26 at the home position. The operation is terminated (step S19).

  According to the embodiment described above, based on the first threshold value set according to the type of sample, the suction amount or discharge amount of the sample, and the discharge amount of the cleaning liquid, the sample dispensing probe 16 at the time of dispensing the sample. Whether or not is in a normal state is determined. When it is determined that the sample is in an abnormal state, the dispensing of the sample can be stopped. Thereby, deterioration of analysis data can be prevented beforehand.

  Further, when it is determined that the state is abnormal based on the first threshold, based on the second threshold set in accordance with the type of the sample, the suction amount or discharge amount of the sample, and the discharge amount of the cleaning liquid. Then, it is determined whether or not the sample dispensing probe 16 is in a normal state at the time of dispensing the sample after discharging the cleaning liquid, and if the sample is in an abnormal state, the subsequent dispensing of the sample is stopped. it can. Thereby, deterioration of analysis data can be prevented beforehand.

  Furthermore, when it is determined that the state is abnormal based on the first threshold, the sample dispensing probe is used for cleaning after the determination based on the third threshold set according to the discharge amount of the cleaning liquid. Whether or not 16 is in a normal state is determined, and if it is in an abnormal state, the subsequent dispensing of the sample can be stopped. Thereby, deterioration of analysis data can be prevented beforehand.

  As described above, it is possible to prevent deterioration of analysis data due to clogging of the sample dispensing probe 16.

  Although several embodiments of the present invention have been described, these embodiments are presented by way of example and are not intended to limit the scope of the invention. These novel embodiments can be implemented in various other forms, and various omissions, replacements, and changes can be made without departing from the scope of the invention. These embodiments and modifications thereof are included in the scope and gist of the invention, and are included in the invention described in the claims and the equivalents thereof.

3 reaction container 16 sample dispensing probe 17 sample container 18 dispensing unit 19 pressure detector 22 tube 26 analysis unit 27 drive unit 28 analysis control unit 30 dispensing monitor unit 31 collection unit 32 threshold storage unit 33 determination unit

Claims (7)

In an automatic analyzer that dispenses samples and reagents into a reaction vessel and measures the mixture,
A dispensing probe for aspirating and discharging the sample and discharging a cleaning liquid;
A detector for detecting a pressure in the dispensing probe when any one of the sample suction, the sample discharge, or the cleaning liquid discharge is performed;
Determining whether or not the dispensing probe is in a normal state based on a pressure when the one detected by the detector is being performed and a first threshold set in advance, and the dispensing probe; Is determined to be in an abnormal state, after the one is performed, based on the pressure when the one detected by the detector is performed and the second threshold value set in advance. An automatic analyzer comprising: a determination unit that determines whether or not a probe is in a normal state. The determination unit makes a determination based on a first threshold value set according to a pressure and a suction amount when the sample detected by the detector is sucked, and the dispensing probe is in an abnormal state. After the determination that the sample is aspirated and the sample is aspirated, a determination is made based on the second threshold value set according to the pressure and the aspiration amount when the sample detected by the detector is aspirated. ,
When the sample is aspirated, the second threshold is a pressure value larger than the first threshold when the sample is aspirated when the first threshold is used. The automatic analyzer according to claim 1. The determination unit makes a determination based on a first threshold value set according to a pressure and a discharge amount when the sample detected by the detector is discharged, and the dispensing probe is in an abnormal state. After determining that there is a sample and discharging the sample, the determination is made based on a second threshold value set according to the pressure and discharge amount when the sample detected by the detector is discharged. ,
The second threshold value used for the determination at the time of discharging the sample is a pressure value smaller than the first threshold value when it is the same as the discharge amount of the sample when the first threshold value is used. The automatic analyzer according to claim 1. The determination unit makes a determination based on a first threshold set according to a pressure and a discharge amount when the cleaning liquid detected by the detector is discharged, and the dispensing probe is in an abnormal state. After determining that there is a discharge of the cleaning liquid, the determination is made based on a second threshold value set according to the pressure and discharge amount when the cleaning liquid detected by the detector is discharged. ,
The second threshold value used for the determination at the time of discharging the cleaning liquid is a pressure value smaller than the first threshold value when it is the same as the discharge amount of the cleaning liquid when the first threshold value is used. The automatic analyzer according to claim 1.   The dispensing probe discharges the cleaning liquid after it is determined that the dispensing probe is in an abnormal state based on the pressure when the one is performed and the first threshold value. The automatic analyzer according to any one of claims 1 to 3.   When the dispensing probe is determined to be in an abnormal state based on the pressure when the one is performed and the second threshold value, the sample aspiration after the determination is made The automatic analyzer according to any one of claims 1 to 4, wherein the discharge is stopped. The dispensing probe is determined to be in an abnormal state based on a first threshold set according to the pressure and the suction amount when the sample is sucked, and the sample to be dispensed after the determination is made If not, discharge the cleaning solution
The determination unit determines whether or not the dispensing probe is in a normal state based on a third threshold set in advance according to the pressure and discharge amount when the cleaning liquid is discharged. The automatic analyzer according to claim 2, wherein

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