JP2007240328A - Dispensing device and dispensation method - Google Patents

Dispensing device and dispensation method Download PDF

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JP2007240328A
JP2007240328A JP2006063239A JP2006063239A JP2007240328A JP 2007240328 A JP2007240328 A JP 2007240328A JP 2006063239 A JP2006063239 A JP 2006063239A JP 2006063239 A JP2006063239 A JP 2006063239A JP 2007240328 A JP2007240328 A JP 2007240328A
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clogging
dispensing
sample
liquid sample
nozzle
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Isao Saegusa
勲 三枝
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Olympus Corp
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Olympus Corp
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Abstract

<P>PROBLEM TO BE SOLVED: To determine whether clogging is caused by a liquid sample or by a sample probe, when detecting clogging of the sample probe. <P>SOLUTION: When detecting clogging of the sample probe by a clogging detection means when sucking the liquid sample (S2:Yes), clogging of the sample probe is detected (S6) by the clogging detection means when discharging cleaning water (S5) after discharging the liquid sample sucked by the sample probe. Resultantly, it can be determined whether clogging is caused by the sample probe or not. When clogging of the sample probe is not detected by the clogging detection means when discharging the cleaning water (S6:No), the same liquid sample is sucked again by the sample probe (S10), and clogging of the sample probe is detected by the clogging detection means (S2). Resultantly, it can be determined whether clogging is caused by the liquid sample or not. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

本発明は、例えば、血液や尿などのサンプル(液体試料)を分析する分析装置に用いられる分注装置および分注方法に関するものである。   The present invention relates to a dispensing device and a dispensing method used in an analyzer that analyzes a sample (liquid sample) such as blood or urine.

生化学などの自動分析装置においては、分注ポンプおよびこれに連結されたサンプルプローブを有する分注装置を設け、サンプルプローブをサンプル吸引位置、サンプル吐出位置およびプローブ洗浄位置に移動可能として、分注ポンプを吸排動作させることにより、サンプル容器から所定量のサンプルを反応容器に分注するようにしている。この種の自動分析装置においては、サンプルとして、血清あるいは血漿が使用されるが、前記サンプル中にはフィブリンなどの固形物が存在するため、その固形物がサンプルプローブやそれに連結されている管路に詰まる場合がある。サンプルプローブに詰まりが生じると、所定量のサンプルを反応容器に分注できないため、分析結果に重大な悪影響を及ぼすことになる。一方、サンプルの吸引時にサンプルプローブがサンプル液面に浸漬せず、サンプルを吸引しない空吸引の場合もある。空吸引が生じると、サンプルを反応容器に分注できないため、分析結果に重大な悪影響を及ぼすことになる。   In automated analyzers such as biochemistry, a dispensing device with a dispensing pump and a sample probe connected to the dispensing pump is provided, and the sample probe can be moved to the sample suction position, the sample discharge position, and the probe washing position, and dispensed. By sucking and discharging the pump, a predetermined amount of sample is dispensed from the sample container into the reaction container. In this type of automatic analyzer, serum or plasma is used as a sample, but since solids such as fibrin are present in the sample, the solids are connected to the sample probe and a pipe connected thereto. May clog. When the sample probe is clogged, a predetermined amount of sample cannot be dispensed into the reaction container, which has a serious adverse effect on the analysis result. On the other hand, there is a case in which the sample probe is not immersed in the sample liquid surface when the sample is sucked and the sample is not sucked. When empty aspiration occurs, the sample cannot be dispensed into the reaction container, which has a serious adverse effect on the analysis result.

このような不具合に対して従来の分注装置では、サンプルプローブ,分注シリンジを含む分注流路系に圧力センサを接続し、サンプル分注時の圧力変動波形を測定して吸引動作が正常に行われたか否かの判定を行う。そして、吸引異常を複数の原因に分類し、それぞれの原因に応じた対策動作を行う(例えば、特許文献1参照)。   For such problems, conventional dispensing devices connect a pressure sensor to the dispensing flow path system including the sample probe and dispensing syringe, and measure the pressure fluctuation waveform during sample dispensing to ensure normal suction operation. It is determined whether or not it has been done. Then, the suction abnormality is classified into a plurality of causes, and a countermeasure operation corresponding to each cause is performed (for example, see Patent Document 1).

特開2002−333449号公報JP 2002-333449 A

従来の分注装置では、吸引異常としてサンプルプローブに詰まりが発生した場合、対策動作として吸引した液体試料を反応容器に吐出するとともに、再度同じ液体試料を吸引して別の反応容器に吐出して分析を行っている。しかし、従来の自動分析装置では、詰まりの原因が、サンプルプローブにあるか、または液体試料にあるかを切り分けていない。このため、液体試料を吐出した反応容器を洗浄する場合に洗浄用ノズルで液体試料を吸引するが、液体試料が詰まりの原因である場合に、当該液体試料によって洗浄用ノズルが詰まるおそれがある。一方、詰まりの原因がサンプルプローブである場合には、再度吸引した液体試料が無駄になってしまう。この結果、装置を止めて原因追究する時間を要することになる。   In a conventional dispensing device, when a sample probe is clogged due to an abnormal suction, as a countermeasure action, the aspirated liquid sample is discharged into a reaction container, and the same liquid sample is again aspirated and discharged into another reaction container. Analyzing. However, the conventional automatic analyzer does not distinguish whether the cause of the clogging is in the sample probe or the liquid sample. For this reason, when cleaning the reaction vessel from which the liquid sample has been discharged, the liquid sample is sucked by the cleaning nozzle. However, when the liquid sample is a cause of clogging, the cleaning nozzle may be clogged by the liquid sample. On the other hand, when the cause of the clogging is a sample probe, the liquid sample sucked again is wasted. As a result, it takes time to stop the device and investigate the cause.

本発明は、上記に鑑みてなされたものであって、分注ノズルの詰まりを検知した場合に、詰まりの原因が液体試料または分注ノズルにあるかを判定することができる分注装置および分注方法を提供することを目的とする。   The present invention has been made in view of the above, and when a clogging of a dispensing nozzle is detected, a dispensing apparatus and a dispensing device that can determine whether the clogging is caused by a liquid sample or a dispensing nozzle The purpose is to provide an ordering method.

上述した課題を解決し、目的を達成するために、本発明の請求項1に係る分注装置は、液体試料を吸引・吐出する分注ノズルと、当該分注ノズルの詰まりを検知する詰まり検知手段とを備えた分注装置において、液体試薬の吸引時に前記詰まり検知手段によって分注ノズルの詰まりを検知した場合、前記分注ノズルで吸引した液体試料を吐出させた後の洗浄水の吐出時に前記詰まり検知手段によって分注ノズルの詰まりを検知する分注制御部を備えたことを特徴とする。   In order to solve the above-described problems and achieve the object, a dispensing apparatus according to claim 1 of the present invention includes a dispensing nozzle that sucks and discharges a liquid sample, and clogging detection that detects clogging of the dispensing nozzle. The clogging of the dispensing nozzle is detected by the clogging detection means when the liquid reagent is aspirated, and when the washing water is discharged after discharging the liquid sample aspirated by the dispensing nozzle. A dispensing control unit for detecting clogging of the dispensing nozzle by the clogging detecting means is provided.

本発明の請求項2に係る分注装置は、上記請求項1において、前記分注制御部は、洗浄水の吐出時に詰まり検知手段によって分注ノズルの詰まりを検知しなかった場合、分注ノズルで再度同じ液体試料を吸引させて前記詰まり検知手段によって分注ノズルの詰まりを検知することを特徴とする。   A dispensing apparatus according to a second aspect of the present invention is the dispensing nozzle according to the first aspect, wherein the dispensing control unit does not detect clogging of the dispensing nozzle by the clogging detecting means when discharging the cleaning water. Then, the same liquid sample is again sucked and clogging of the dispensing nozzle is detected by the clogging detecting means.

本発明の請求項3に係る分注装置は、上記請求項2において、前記分注制御部は、同じ液体試料の再度の吸引時に詰まり検知手段によって分注ノズルの詰まりを検知した場合、当該分注ノズルを洗浄させた後に他の液体試料の分注を行わせることを特徴とする。   A dispensing apparatus according to a third aspect of the present invention is the dispensing apparatus according to the second aspect, wherein the dispensing control unit detects the clogging of the dispensing nozzle by the clogging detecting means when the same liquid sample is sucked again. It is characterized in that another liquid sample is dispensed after the injection nozzle is washed.

本発明の請求項4に係る分注装置は、上記請求項1〜3のいずれか一つにおいて、洗浄に伴う液体試料の吐出を所定の洗浄位置で行わせることを特徴とする。   A dispensing apparatus according to a fourth aspect of the present invention is characterized in that, in any one of the first to third aspects, the liquid sample accompanying the cleaning is discharged at a predetermined cleaning position.

本発明の請求項5に係る分注方法は、液体試料を吸引・吐出する分注ノズルと、当該分注ノズルの詰まりを検知する詰まり検知手段とを備えた分注装置での分注方法において、液体試薬の吸引時に前記詰まり検知手段によって分注ノズルの詰まりを検知した場合、前記分注ノズルで吸引した液体試料を吐出させた後の洗浄水の吐出時に前記詰まり検知手段によって分注ノズルの詰まりを検知する工程を含むことを特徴とする。   A dispensing method according to claim 5 of the present invention is a dispensing method in a dispensing apparatus that includes a dispensing nozzle that sucks and discharges a liquid sample and a clogging detection unit that detects clogging of the dispensing nozzle. When clogging of the dispensing nozzle is detected by the clogging detection means during the suction of the liquid reagent, the clogging detection means detects the clogging nozzle when the cleaning water is discharged after discharging the liquid sample sucked by the dispensing nozzle. The method includes a step of detecting clogging.

本発明の請求項6に係る分注方法は、上記請求項5において、洗浄水の吐出時に詰まり検知手段によって分注ノズルの詰まりを検知しなかった場合、分注ノズルで再度同じ液体試料を吸引させて前記詰まり検知手段によって分注ノズルの詰まりを検知する工程をさらに含むことを特徴とする。   A dispensing method according to a sixth aspect of the present invention is the dispensing method according to the fifth aspect, wherein when the clogging detection means does not detect clogging of the dispensing nozzle when the washing water is discharged, the same liquid sample is sucked again by the dispensing nozzle. And a step of detecting clogging of the dispensing nozzle by the clogging detecting means.

本発明の請求項7に係る分注方法は、上記請求項6において、同じ液体試料の再度の吸引時に詰まり検知手段によって分注ノズルの詰まりを検知した場合、当該分注ノズルを洗浄させた後に他の液体試料の分注を行わせる工程をさらに含むことを特徴とする。   In the dispensing method according to claim 7 of the present invention, in the above claim 6, when clogging of the dispensing nozzle is detected by the clogging detecting means when the same liquid sample is sucked again, the dispensing nozzle is washed. The method further includes a step of dispensing another liquid sample.

本発明に係る分注装置および分注方法は、液体試薬の吸引時に詰まり検知手段によって分注ノズルの詰まりを検知した場合、分注ノズルで吸引した液体試料を吐出させた後の洗浄水の吐出時に詰まり検知手段によって分注ノズルの詰まりを検知する。この結果、分注ノズルが原因の詰まりか否かを判定することができる。   In the dispensing apparatus and the dispensing method according to the present invention, when clogging of the dispensing nozzle is detected by the clogging detecting means during the suction of the liquid reagent, the cleaning water is discharged after the liquid sample sucked by the dispensing nozzle is discharged. Sometimes the clogging detection means detects clogging of the dispensing nozzle. As a result, it can be determined whether or not the dispensing nozzle is clogged.

また、洗浄水の吐出時に詰まり検知手段によって分注ノズルの詰まりを検知しなかった場合、分注ノズルで再度同じ液体試料を吸引させて詰まり検知手段によって分注ノズルの詰まりを検知する。この結果、液体試料が原因の詰まりか否かを判定することができる。   Further, when clogging of the dispensing nozzle is not detected by the clogging detection means when the cleaning water is discharged, the same liquid sample is again sucked by the dispensing nozzle, and clogging of the dispensing nozzle is detected by the clogging detection means. As a result, it can be determined whether or not the liquid sample is clogged.

また、同じ液体試料の再度の吸引時に詰まり検知手段によって分注ノズルの詰まりを検知した場合、分注ノズルを洗浄させた後に他の液体試料の分注を行わせる。この結果、液体試料が原因の詰まりを判定した場合に、分注動作を止めることなく他の検体試料の分析を行うことができる。   Further, when clogging of the dispensing nozzle is detected by the clogging detecting means when the same liquid sample is sucked again, another liquid sample is dispensed after the dispensing nozzle is washed. As a result, when the clogging caused by the liquid sample is determined, it is possible to analyze another specimen sample without stopping the dispensing operation.

また、洗浄に伴う液体試料の吐出を所定の洗浄位置で行わせる。すなわち、分析機の反応容器に詰まりの原因と疑える液体試料の吐出を行わない。この結果、分析機において反応容器を洗浄する洗浄用ノズルの詰まりを回避することができる。   Further, the liquid sample accompanying the cleaning is discharged at a predetermined cleaning position. That is, the liquid sample suspected of causing the clogging in the reaction container of the analyzer is not discharged. As a result, it is possible to avoid clogging of the washing nozzle for washing the reaction container in the analyzer.

以下に添付図面を参照して、本発明に係る分注装置および分注方法の好適な実施の形態を詳細に説明する。なお、この実施の形態によりこの発明が限定されるものではない。   Exemplary embodiments of a dispensing apparatus and a dispensing method according to the present invention will be described below in detail with reference to the accompanying drawings. Note that the present invention is not limited to the embodiments.

図1は本発明に係る分注装置の一例を示す概略図である。分注装置は、血液や尿などのサンプル(液体試料)を分析する分析装置に用いられ、サンプル容器C1から所定量のサンプルを反応容器C2に分注するためのものである。図1に示すように分注装置は、分注手段として、サンプルプローブ(分注ノズル)1と分注ポンプ2とを管路3で連結してある。   FIG. 1 is a schematic view showing an example of a dispensing apparatus according to the present invention. The dispensing device is used in an analyzer that analyzes a sample (liquid sample) such as blood or urine, and dispenses a predetermined amount of sample from the sample container C1 to the reaction container C2. As shown in FIG. 1, in the dispensing apparatus, a sample probe (dispensing nozzle) 1 and a dispensing pump 2 are connected by a conduit 3 as dispensing means.

サンプルプローブ1は、プローブ駆動手段1aによって、サンプルの入ったサンプル容器C1があるサンプル吸引位置、サンプルを反応させる反応容器C2があるサンプル吐出位置、洗浄槽C3がある洗浄位置などに水平移動可能に設けてあり、さらにプローブ駆動手段1aによって、各位置で昇降移動可能に設けてある。   The sample probe 1 can be moved horizontally by the probe driving means 1a to a sample suction position where the sample container C1 containing the sample is located, a sample discharge position where the reaction container C2 for reacting the sample is present, a washing position where the washing tank C3 is located, and the like. Furthermore, it is provided to be movable up and down at each position by the probe driving means 1a.

分注ポンプ2は、例えばシリンジからなり、分注ポンプ駆動手段2aによって吸排動作する。   The dispensing pump 2 is composed of, for example, a syringe, and performs a suction / discharge operation by the dispensing pump driving means 2a.

管路3は、サンプルプローブ1の移動を妨げることのない可撓性を有したチューブからなる。管路3の内部には、サンプルプローブ1および分注ポンプ2を含み洗浄水が充填してある。洗浄水には、空気を除かれた脱気水が用いられている。管路3に充填された洗浄水は、タンク4に収容してある。タンク4は、分注ポンプ2と管路5で連結してある。管路5は、管路3と同様のチューブである。この管路5には、ポンプ6および電磁弁7が設けてある。すなわち、タンク4に収容した洗浄水は、電磁弁7を開けてポンプ6を駆動することによって管路5を経て分注ポンプ2,管路3およびサンプルプローブ1に充填される。   The conduit 3 is made of a flexible tube that does not hinder the movement of the sample probe 1. The inside of the pipe line 3 is filled with washing water including the sample probe 1 and the dispensing pump 2. As the washing water, deaerated water from which air has been removed is used. The washing water filled in the pipe line 3 is accommodated in the tank 4. The tank 4 is connected to the dispensing pump 2 by a pipe line 5. The pipe 5 is a tube similar to the pipe 3. The pipe 5 is provided with a pump 6 and an electromagnetic valve 7. That is, the wash water stored in the tank 4 is filled into the dispensing pump 2, the pipe 3 and the sample probe 1 through the pipe 5 by opening the electromagnetic valve 7 and driving the pump 6.

図1に示すように上述したプローブ駆動手段1a,分注ポンプ駆動手段2a,ポンプ6および電磁弁7は、分注制御部8によって統括して制御される。すなわち、分注制御部8は、分注動作においてプローブ駆動手段1aを制御してサンプルプローブ1を移動させ、さらに分注ポンプ駆動手段2aを制御して分注ポンプ2を吸排動作させる。これにより、分注装置は、サンプルプローブ1でサンプル容器C1からサンプルを吸引し、反応容器C2に吐出する。一方、分注制御部8は、洗浄動作においてプローブ駆動手段1aを制御してサンプルプローブ1を移動させ、さらに分注ポンプ駆動手段2aを制御して分注ポンプ2を吸排動作させる。これにより、分注装置は、サンプルプローブ1で吸引したサンプルを洗浄槽C3に吐出し、サンプルプローブ1を洗浄する。   As shown in FIG. 1, the probe driving means 1 a, the dispensing pump driving means 2 a, the pump 6 and the electromagnetic valve 7 described above are controlled by the dispensing control unit 8. That is, the dispensing control unit 8 controls the probe driving means 1a to move the sample probe 1 in the dispensing operation, and further controls the dispensing pump driving means 2a to cause the dispensing pump 2 to perform suction and discharge operations. Thereby, the dispensing apparatus sucks the sample from the sample container C1 with the sample probe 1 and discharges it to the reaction container C2. On the other hand, the dispensing control unit 8 controls the probe driving unit 1a to move the sample probe 1 in the cleaning operation, and further controls the dispensing pump driving unit 2a to cause the dispensing pump 2 to perform an intake / exhaust operation. Thereby, the dispensing apparatus discharges the sample sucked by the sample probe 1 to the cleaning tank C3 and cleans the sample probe 1.

また、管路5(または管路3)には、圧力検出手段9が設けてある。圧力検出手段(圧力センサ)9は、分注ポンプ2の吸排動作によってサンプルプローブ1で液体試料を吸引・吐出した際に、サンプルプローブ1および分注ポンプ2を含む管路3,5内の洗浄水の圧力を検出して出力するためのものである。   Further, pressure detection means 9 is provided in the pipe line 5 (or the pipe line 3). The pressure detection means (pressure sensor) 9 cleans the pipelines 3 and 5 including the sample probe 1 and the dispensing pump 2 when the sample probe 1 sucks and discharges the liquid sample by the suction / discharge operation of the dispensing pump 2. It is for detecting and outputting the pressure of water.

圧力検出手段9の出力は、詰まり検知手段10に供給される。詰まり検知手段10は、圧力検出手段9からの出力をデジタル信号に変換するA/D変換器11,増幅回路12,積分演算回路13および判別回路14を有している。A/D変換器11は、圧力検出手段9の出力をデジタル信号に変換する。増幅回路12は、A/D変換器11から出力されたデジタル信号を増幅する。積分演算回路13は、デジタル信号を積分値に演算する。判別回路14は、所定の閾値等を格納してあり、積分演算回路13の出力した積分値と閾値との比較を行い、当該比較に基づきサンプルプローブ1の吸引状態の判別結果を出力する。この判別結果は、分注制御部8に供給される。分注制御部8では、判別結果に応じて、正常吸引であれば分析機を統括制御する分析制御部(図示せず)に対して分析を継続する旨の信号を出力し、異常吸引(詰まり・空吸引)であれば、サンプルプローブ1の洗浄を得て、同じ液体試料の再分析や、別の液体試料の分析を実行する。なお、A/D変換器11と増幅回路12とを逆に設け、圧力検出手段9からの出力を増幅回路12で増幅した後にA/D変換器11で変換したデジタル信号を積分演算回路13に供給してもよい。   The output of the pressure detection means 9 is supplied to the clogging detection means 10. The clogging detection means 10 includes an A / D converter 11 that converts an output from the pressure detection means 9 into a digital signal, an amplification circuit 12, an integration calculation circuit 13, and a determination circuit 14. The A / D converter 11 converts the output of the pressure detection means 9 into a digital signal. The amplifier circuit 12 amplifies the digital signal output from the A / D converter 11. The integration calculation circuit 13 calculates a digital signal into an integral value. The determination circuit 14 stores a predetermined threshold value and the like, compares the integrated value output from the integration calculation circuit 13 with the threshold value, and outputs the determination result of the suction state of the sample probe 1 based on the comparison. The determination result is supplied to the dispensing control unit 8. In accordance with the determination result, the dispensing control unit 8 outputs a signal indicating that the analysis is continued to an analysis control unit (not shown) that controls the analyzer if it is normal suction, and performs abnormal suction (clogging). If it is empty suction, the sample probe 1 is washed, and the same liquid sample is reanalyzed or another liquid sample is analyzed. The A / D converter 11 and the amplifier circuit 12 are provided in reverse, and the digital signal converted by the A / D converter 11 after the output from the pressure detection means 9 is amplified by the amplifier circuit 12 is supplied to the integration arithmetic circuit 13. You may supply.

図2は分注制御部の動作を示すフローチャートである。正常時において、分注制御部8は、新規液体試薬を吸引して吸引回数N=1とカウントし(ステップS1)、この液体試薬の吸引時に詰まり検知手段10によってサンプルプローブ1の詰まりを検知しなかった場合(ステップS2:No)、サンプルプローブ1で吸引した液体試料を反応容器C2に吐出して吸引回数N=0とリセットする(ステップS3)。すなわち、サンプルプローブ1で吸引した液体試料を用いて分析を行う。この場合、再びステップ1に戻って新規液体試料の分析を行う。   FIG. 2 is a flowchart showing the operation of the dispensing control unit. At the normal time, the dispensing control unit 8 sucks the new liquid reagent and counts the number of times of suction N = 1 (step S1), and detects the clogging of the sample probe 1 by the clogging detecting means 10 when the liquid reagent is aspirated. If not (step S2: No), the liquid sample sucked by the sample probe 1 is discharged into the reaction container C2 and resets the number of times of suction N = 0 (step S3). That is, analysis is performed using the liquid sample sucked by the sample probe 1. In this case, the process returns to Step 1 again to analyze the new liquid sample.

一方、異常吸引時において、分注制御部8は、液体試薬の吸引時に詰まり検知手段10によってサンプルプローブ1の詰まりを検知した場合(ステップS2:Yes)、サンプルプローブ1で吸引した液体試料を洗浄槽C3に吐出させる(ステップS4)。すなわち、液体試料を破棄する。次いで、サンプルプローブ1の洗浄を行う(ステップS5)。サンプルプローブ1の洗浄は、電磁弁7を開けてポンプ6を駆動することで充填してある洗浄水をサンプルプローブ1から吐出させる。この洗浄水の吐出時に詰まり検知手段10によってサンプルプローブ1の詰まりを検知した場合(ステップS6:Yes)、分注制御部8は、サンプルプローブ1が原因(サンプルプローブの異常)の詰まりと判定し(ステップS7)、分注動作を停止してアラームを出して(ステップS8)、サンプルプローブ1の点検のため分注動作を終了する。   On the other hand, at the time of abnormal suction, the dispensing control unit 8 cleans the liquid sample sucked by the sample probe 1 when the clogging detecting means 10 detects clogging of the sample probe 1 at the time of sucking the liquid reagent (step S2: Yes). It is made to discharge to the tank C3 (step S4). That is, the liquid sample is discarded. Next, the sample probe 1 is washed (step S5). The sample probe 1 is washed by opening the electromagnetic valve 7 and driving the pump 6 to discharge the filled washing water from the sample probe 1. When clogging of the sample probe 1 is detected by the clogging detection means 10 at the time of discharge of the washing water (step S6: Yes), the dispensing control unit 8 determines that the sample probe 1 is clogged due to the cause (abnormality of the sample probe). (Step S7), the dispensing operation is stopped, an alarm is issued (Step S8), and the dispensing operation is terminated for checking the sample probe 1.

また、ステップS6において、洗浄水の吐出時に詰まり検知手段10によってサンプルプローブ1の詰まりの検出がなく(ステップS6:No)、吸引回数が1回目であれば(ステップS9:Yes)、分注制御部8は、同じ液体試料を吸引して吸引回数N=2とカウントし(ステップS10)、ステップS2に戻る。すなわち、同じ液体試料の再度の吸引時に詰まり検知手段10によってサンプルプローブ1の詰まりを検出する。ここで、同じ液体試料の再度の吸引時に詰まり検知手段10によってサンプルプローブ1の詰まりを検知しなかった場合(ステップS2:No)、分注制御部8は、通常時に戻ってサンプルプローブ1で吸引した液体試料を反応容器C2に吐出させる(ステップS3)。また、分注制御部8は、同じ液体試料の再度の吸引時に詰まり検知手段10によってサンプルプローブ1の詰まりを検知した場合(ステップS2:Yes)、上記と同様にステップS9に至る。   In step S6, if the clogging detection means 10 does not detect clogging of the sample probe 1 at the time of discharge of cleaning water (step S6: No) and the number of times of suction is the first time (step S9: Yes), dispensing control is performed. The unit 8 sucks the same liquid sample, counts the number of times of suction N = 2 (step S10), and returns to step S2. That is, the clogging detection means 10 detects clogging of the sample probe 1 when the same liquid sample is sucked again. Here, when clogging of the sample probe 1 is not detected by the clogging detecting means 10 when the same liquid sample is sucked again (step S2: No), the dispensing control unit 8 returns to the normal time and sucks with the sample probe 1. The liquid sample thus discharged is discharged into the reaction container C2 (step S3). Further, when the clogging detection unit 10 detects clogging of the sample probe 1 when the same liquid sample is aspirated again (step S2: Yes), the dispensing control unit 8 reaches step S9 as described above.

ステップS9において、液体試料の吸引回数が1回目(N=1)でない場合(ステップS9:No)、すなわち、同じ液体試料の吸引回数が2回目(N=2)である場合、分注制御部8は、吸引していた液体試料が原因(液体試料の異常)の詰まりと判定し(ステップS11)、他の液体試料を吸引して吸引回数N=1とカウントし(ステップS12)、ステップS2に戻る。   In step S9, when the number of times the liquid sample is aspirated is not the first (N = 1) (step S9: No), that is, when the number of times the same liquid sample is aspirated is the second (N = 2), the dispensing control unit 8 determines that the liquid sample that has been sucked is clogged (liquid sample abnormality) (step S11), sucks another liquid sample and counts the number of times of suction N = 1 (step S12), and step S2 Return to.

このように、上述した分注装置では、液体試薬の吸引時に詰まり検知手段10によってサンプルプローブ1の詰まりを検知した場合、サンプルプローブ1で吸引した液体試料を吐出させた後の洗浄水の吐出時に詰まり検知手段10によってサンプルプローブ1の詰まりを検知する。この結果、サンプルプローブ1が原因の詰まりか否かを判定することが可能になる。   Thus, in the dispensing apparatus described above, when clogging of the sample probe 1 is detected by the clogging detection means 10 when the liquid reagent is aspirated, when the cleaning water is discharged after the liquid sample aspirated by the sample probe 1 is discharged. The clogging detection means 10 detects clogging of the sample probe 1. As a result, it is possible to determine whether or not the sample probe 1 is clogged.

また、洗浄水の吐出時に詰まり検知手段10によってサンプルプローブ1の詰まりを検知しなかった場合、サンプルプローブ1で再度同じ液体試料を吸引させて詰まり検知手段10によってサンプルプローブ1の詰まりを検知する。この結果、液体試料が原因の詰まりか否かを判定することが可能になる。   Further, when clogging of the sample probe 1 is not detected by the clogging detection means 10 when the cleaning water is discharged, the same liquid sample is again sucked by the sample probe 1 and the clogging of the sample probe 1 is detected by the clogging detection means 10. As a result, it is possible to determine whether or not the liquid sample is clogged.

また、同じ液体試料の再度の吸引時に詰まり検知手段10によってサンプルプローブ1の詰まりを検知した場合、サンプルプローブ1を洗浄させた後に他の液体試料の分注を行わせる。この結果、液体試料が原因の詰まりを判定した場合に、分注動作を止めることなく他の検体試料の分析を行うことが可能になる。   When clogging of the sample probe 1 is detected by the clogging detection means 10 when the same liquid sample is sucked again, another liquid sample is dispensed after the sample probe 1 is washed. As a result, when the clogging caused by the liquid sample is determined, it is possible to analyze another specimen sample without stopping the dispensing operation.

また、洗浄に伴う液体試料の吐出を所定の洗浄位置で行わせる。すなわち、分析機の反応容器に詰まりの原因と疑える液体試料の吐出を行わない。この結果、分析機において反応容器を洗浄する洗浄用ノズルの詰まりを回避することが可能になる。   Further, the liquid sample accompanying the cleaning is discharged at a predetermined cleaning position. That is, the liquid sample suspected of causing the clogging in the reaction container of the analyzer is not discharged. As a result, it is possible to avoid clogging of the cleaning nozzle for cleaning the reaction container in the analyzer.

本発明に係る分注装置の一例を示す概略図である。It is the schematic which shows an example of the dispensing apparatus which concerns on this invention. 分注制御部の動作を示すフローチャートである。It is a flowchart which shows operation | movement of a dispensing control part.

符号の説明Explanation of symbols

1 サンプルプローブ
1a プローブ駆動手段
2 分注ポンプ
2a 分注ポンプ駆動手段
3 管路
4 タンク
5 管路
6 ポンプ
7 電磁弁
8 分注制御部
9 圧力検出手段
10 詰まり検知手段
11 A/D変換器
12 増幅回路
13 積分演算回路
14 判別回路
15 分析制御部
C1 サンプル容器
C2 反応容器
C3 洗浄槽
DESCRIPTION OF SYMBOLS 1 Sample probe 1a Probe drive means 2 Dispensing pump 2a Dispensing pump drive means 3 Pipe line 4 Tank 5 Pipe line 6 Pump 7 Solenoid valve 8 Dispensing control part 9 Pressure detection means 10 Clogging detection means 11 A / D converter 12 Amplification circuit 13 Integral operation circuit 14 Discrimination circuit 15 Analysis control unit C1 Sample container C2 Reaction container C3 Washing tank

Claims (7)

液体試料を吸引・吐出する分注ノズルと、当該分注ノズルの詰まりを検知する詰まり検知手段とを備えた分注装置において、
液体試薬の吸引時に前記詰まり検知手段によって分注ノズルの詰まりを検知した場合、前記分注ノズルで吸引した液体試料を吐出させた後の洗浄水の吐出時に前記詰まり検知手段によって分注ノズルの詰まりを検知する分注制御部を備えたことを特徴とする分注装置。
In a dispensing apparatus comprising a dispensing nozzle for sucking and discharging a liquid sample, and a clogging detecting means for detecting clogging of the dispensing nozzle,
When clogging of the dispensing nozzle is detected by the clogging detection means when the liquid reagent is aspirated, the clogging nozzle is clogged by the clogging detection means when the cleaning water is discharged after discharging the liquid sample sucked by the dispensing nozzle. A dispensing apparatus comprising a dispensing control unit for detecting a mist.
前記分注制御部は、洗浄水の吐出時に詰まり検知手段によって分注ノズルの詰まりを検知しなかった場合、分注ノズルで再度同じ液体試料を吸引させて前記詰まり検知手段によって分注ノズルの詰まりを検知することを特徴とする請求項1に記載の分注装置。   When the clogging detection means does not detect clogging of the dispensing nozzle when the cleaning water is discharged, the dispensing control unit sucks the same liquid sample again with the dispensing nozzle and clogs the dispensing nozzle by the clogging detection means. The dispensing device according to claim 1, wherein the dispensing device is detected. 前記分注制御部は、同じ液体試料の再度の吸引時に詰まり検知手段によって分注ノズルの詰まりを検知した場合、当該分注ノズルを洗浄させた後に他の液体試料の分注を行わせることを特徴とする請求項2に記載の分注装置。   When the clogging detection means detects clogging of the dispensing nozzle when the same liquid sample is aspirated again, the dispensing control unit causes the dispensing nozzle to perform dispensing after washing the dispensing nozzle. The dispensing device according to claim 2, wherein the dispensing device is characterized in that 洗浄に伴う液体試料の吐出を所定の洗浄位置で行わせることを特徴とする請求項1〜3のいずれか一つに記載の分注装置。   The dispensing apparatus according to any one of claims 1 to 3, wherein the liquid sample accompanying the cleaning is discharged at a predetermined cleaning position. 液体試料を吸引・吐出する分注ノズルと、当該分注ノズルの詰まりを検知する詰まり検知手段とを備えた分注装置での分注方法において、
液体試薬の吸引時に前記詰まり検知手段によって分注ノズルの詰まりを検知した場合、前記分注ノズルで吸引した液体試料を吐出させた後の洗浄水の吐出時に前記詰まり検知手段によって分注ノズルの詰まりを検知する工程を含むことを特徴とする分注方法。
In a dispensing method with a dispensing nozzle comprising a dispensing nozzle for sucking and discharging a liquid sample and a clogging detecting means for detecting clogging of the dispensing nozzle,
When clogging of the dispensing nozzle is detected by the clogging detection means when the liquid reagent is aspirated, the clogging nozzle is clogged by the clogging detection means when the cleaning water is discharged after discharging the liquid sample sucked by the dispensing nozzle. The dispensing method characterized by including the process of detecting.
洗浄水の吐出時に詰まり検知手段によって分注ノズルの詰まりを検知しなかった場合、分注ノズルで再度同じ液体試料を吸引させて前記詰まり検知手段によって分注ノズルの詰まりを検知する工程をさらに含むことを特徴とする請求項5に記載の分注方法。   When clogging of the dispensing nozzle is not detected by the clogging detection means when discharging the cleaning water, the method further includes a step of sucking the same liquid sample again by the dispensing nozzle and detecting clogging of the dispensing nozzle by the clogging detection means. The dispensing method according to claim 5, wherein: 同じ液体試料の再度の吸引時に詰まり検知手段によって分注ノズルの詰まりを検知した場合、当該分注ノズルを洗浄させた後に他の液体試料の分注を行わせる工程をさらに含むことを特徴とする請求項6に記載の分注方法。   When clogging of the dispensing nozzle is detected by the clogging detecting means when the same liquid sample is sucked again, the liquid nozzle further includes a step of dispensing another liquid sample after washing the dispensing nozzle. The dispensing method according to claim 6.
JP2006063239A 2006-03-08 2006-03-08 Dispensing device and dispensation method Pending JP2007240328A (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010001646A1 (en) * 2008-07-01 2010-01-07 オリンパス株式会社 Dispensing nozzle bore blot detecting method, and specimen dispensing apparatus
JP2014157073A (en) * 2013-02-15 2014-08-28 Toshiba Corp Automatic analyzer
EP3570043A1 (en) 2018-05-16 2019-11-20 Jeol Ltd. Automatic analyzer and automatic analysis method
US11366129B2 (en) 2018-05-16 2022-06-21 Jeol Ltd. Automatic analyzer and automatic analysis method
CN115135358A (en) * 2020-02-27 2022-09-30 美国西门子医学诊断股份有限公司 Automatic sensor tracking verification using machine learning
JP2023516295A (en) * 2020-02-27 2023-04-19 シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレイテッド Automatic sensor trace verification using machine learning
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US12124325B2 (en) 2021-02-25 2024-10-22 Siemens Healthcare Diagnostics Inc. Automatic sensor trace validation using machine learning

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