JP6338898B2 - Automatic analyzer - Google Patents

Description

  Embodiments described herein relate generally to an automatic analyzer that dispenses a sample and a reagent into a reaction container and measures a mixed solution of the sample and the reagent dispensed into the reaction container.

  The automatic analyzer is intended for biochemical test items and immunological test items, etc., and changes in color and turbidity caused by the reaction of the mixture of the sample collected from the specimen and the reagent of each test item are measured with a spectrophotometer or a ratio. Optically measured with a photometric unit such as a turbidimeter. Thereby, the analysis data represented by the concentration of each test item component contained in the sample, the activity of the enzyme, and the like are generated.

  In this automatic analyzer, each inspection item set for each sample is inspected. When the sample rack holding the sample container for storing the sample is placed on the sampler, the sample dispensing probe sucks the sample in the sample container and discharges it to the reaction container, and the reagent dispensing probe is placed in the reagent container. The reagent is aspirated and discharged into the reaction container. Next, the measurement unit measures the mixed solution of the sample and the reagent in the reaction container.

  By the way, serum or plasma separated from blood is often used as a sample. This sample may contain insoluble matter such as fibrin generated from blood components. When insoluble matter adheres to the sample dispensing probe that sucks the sample and clogging occurs, the sample dispensing accuracy It will cause the decrease. In order to solve this problem, there is known an automatic analyzer that is provided with a pressure sensor that detects the pressure in the sample dispensing probe and can detect clogging of the sample dispensing probe.

  Thus, when a problem arises in the dispensing of the sample, it is necessary to check the sample in which the problem has occurred, remove the cause, and inspect again.

JP 2010-38578 A

  However, when a plurality of sample racks are densely placed on the sampler, it is necessary to find a sample container that accommodates a sample that requires reexamination from among the plurality of sample containers that are densely arranged. Further, in order to confirm the state of the sample in the sample container, it is necessary to take out the found sample container from the sample rack. For this reason, the work of performing the re-inspection is complicated and difficult.

  The embodiment has been made to solve the above problems, and an object thereof is to provide an automatic analyzer that can reduce the work of re-examination.

In order to achieve the above object, the automatic analyzer according to the embodiment dispenses a sample and a reagent in a sample container into a reaction container, and sucks the sample in the sample container in the automatic analyzer that measures the mixed solution. A sample dispensing probe for dispensing into the reaction vessel, a pressure detector for detecting the pressure in the sample dispensing probe when the sample is being dispensed, and the pressure detector A determination unit that determines whether or not the sample dispensing by the sample dispensing probe is normal based on the pressure detected by the sample dispensing probe, a sample rack that holds the sample container, and the sample rack that is held in the sample rack a sample vessel, and a moving mechanism for moving the sample container containing the by the determination unit dispensing is determined to be abnormal samples above, the sample rack upward by the moving mechanism Characterized in that for holding the sample container in the dynamic position.

The block diagram which shows the structure of the automatic analyzer which concerns on embodiment. The perspective view which shows the structure of the analysis part which concerns on embodiment. The figure which shows the position of a sample dispensing probe when the contact and separation | spacing with a sample are detected by the liquid level detector which concerns on embodiment. The figure which shows the lower stop position of the sample dispensing probe which concerns on embodiment. The top view which shows the structure of the sampler part which concerns on embodiment, and a sample dispensing probe. The figure which shows the structure of the sample rack which concerns on embodiment. The figure which shows the sample container in the sample position in the dispensing position which concerns on embodiment, and the sampler part which hold | maintains this sample container. The figure which shows the position of the sample dispensing probe when the sample which concerns on embodiment is aspirated. The figure which shows the structure of the dispensing unit which concerns on embodiment, and a pressure detector. The flowchart which shows the operation | movement in connection with sample dispensing of the automatic analyzer which concerns on embodiment. The flowchart which shows the operation | movement in connection with sample dispensing of the automatic analyzer which concerns on embodiment. The flowchart which shows the operation | movement in connection with sample dispensing of the automatic analyzer which concerns on embodiment.

  Hereinafter, embodiments will be described with reference to the drawings.

  FIG. 1 is a block diagram illustrating a configuration of an automatic analyzer according to the embodiment. This automatic analyzer 100 dispenses the standard sample of each inspection item or the sample of the test sample and the reagent used for the analysis of each inspection item, measures the mixed solution of the sample and the reagent, and standard data or test data. The analysis part 40 which produces | generates is provided. In addition, a drive unit 41 that moves each analysis unit of the analysis unit 40 and an analysis control unit 45 that controls the drive unit 41 are provided.

  In addition, the automatic analyzer 100 includes a detection unit 50 that detects the state of the analysis unit that dispenses the sample of the analysis unit 40, and normal dispensing of the sample in the analysis unit 40 based on information detected by the detection unit 50. It is provided with the determination part 60 which determines whether it is. Moreover, the data processing part 70 which processes the standard data and test data produced | generated by the analysis part 40, and produces | generates calibration data and analysis data is provided.

  The automatic analyzer 100 further includes an output unit 80 for outputting calibration data and analysis data generated by the data processing unit 70, an analysis parameter for each inspection item, a sample ID for identifying a sample to be inspected for inspection, and this ID And an operation unit 90 for inputting an inspection item or the like for the sample ID. In addition, a system control unit 91 that controls the analysis control unit 45, the detection unit 50, the determination unit 60, the data processing unit 70, and the output unit 80 is provided.

  FIG. 2 is a perspective view showing the configuration of the analysis unit 40. The analysis unit 40 includes a sample container 10 that stores a sample such as a standard sample or a test sample, a sample rack 11 that can hold a plurality of sample containers 10, and a sampler unit 12 on which the sample rack 11 is placed. I have. A reagent container 13 containing a first reagent or a two-reagent system first reagent containing a component that reacts with a component of a test item included in the sample; a reagent rack 14 holding the reagent container 13 movably; And a reagent storage 15 for keeping the first reagent in the reagent container 13 held in the reagent rack 14 cool.

  In addition, a reagent container 16 that stores a second reagent that forms a pair with the first reagent of the two-reagent system, a reagent rack 17 that holds the reagent container 16 so as to be movable, and a reagent container 16 that is held in the reagent rack 17 are kept cold. And a reagent storage 18 for performing the above operation. In addition, a plurality of reaction vessels 19 arranged on the circumference and a reaction disk 20 that holds each reaction vessel 19 in a movable manner are provided.

  In addition, a sample dispensing probe 21 that performs dispensing to suck the sample in the sample container 10 held in the sample rack 11 and discharge it into the reaction container 19, and a sample portion that holds the sample dispensing probe 21 movably. And a note arm 22. In addition, a dispensing unit 23 is provided that supplies a cleaning liquid to the sample dispensing probe 21 to perform washing.

  Also, the first reagent dispensing probe 24 for dispensing the first reagent in the reagent container 13 held in the reagent rack 14 and sucking it into the reaction container 19 and the first reagent dispensing probe 24 are moved. And a first reagent dispensing arm 25 that holds the first reagent. In addition, a second reagent dispensing probe 26 that performs dispensing to suck the second reagent in the reagent container 16 held in the reagent rack 17 and discharge the second reagent into the reaction container 19 from which the first reagent has been discharged; A second reagent dispensing arm 27 that holds the reagent dispensing probe 26 so as to be movable is provided.

  In addition, a measurement unit 28 that measures the liquid mixture by irradiating the reaction container 19 with light, and a cleaning nozzle 29 that cleans the reaction container 19 that has been measured by the measurement unit 28 are provided. Then, the measurement unit 28 irradiates the reaction container 19 with light, and by this irradiation, a mixture of the standard sample and the first reagent or the first and second reagents in the reaction container 19, the test sample and the first reagent or Light transmitted through the mixed liquid of the first and second reagents is detected. Then, the detected signal is processed, for example, standard data represented by absorbance or test data is generated and output to the data processing unit 70.

  The driving unit 41 in FIG. 1 includes first and second moving mechanisms 42 and 43 that are rack moving mechanisms that move the sample rack 11 placed on the sampler unit 12 of the analyzing unit 40, and the sampler unit 12. A third moving mechanism 44 that is a container moving mechanism for moving the sample container 10 held in the sample rack 11 is provided. In addition, a moving mechanism for moving the reagent container 13 and the reagent container 16 by driving the reagent rack 14 and the reagent rack 17 respectively is provided.

  In addition, the drive unit 41 includes a moving mechanism that rotates the reaction disk 20 to move each reaction vessel 19. Further, the sample dispensing arm 22, the first reagent dispensing arm 25, and the second reagent dispensing arm 27 are rotated and driven up and down, respectively, so that the sample dispensing probe 21, the first reagent dispensing probe 24, and the second reagent are driven. A moving mechanism for moving the dispensing probe 26 in the horizontal direction and the vertical direction is provided.

  As shown in FIG. 2, the first moving mechanism 42 moves the sample rack 11 placed on the sampler unit 12 in one direction in the short direction of the sample rack 11, and the longitudinal direction of the sampler unit 12. It moves horizontally in the direction of the arrow L1.

  As shown in FIG. 2, the second moving mechanism 43 moves the sample rack 11 moved by the first moving mechanism 42 in one direction in the longitudinal direction of the sample rack 11 and is perpendicular to the L1 direction. It moves horizontally in the direction of arrow L2, which is a simple direction. Further, the sample rack 11 moved in the L2 direction is horizontally moved in the L1 direction. Further, the sample rack 11 moved in the L1 direction is horizontally moved in the direction of the arrow L3, which is the other direction in the longitudinal direction of the sample rack 11.

  The third moving mechanism 44 is disposed below the sampler unit 12. Then, the sample container 10 holding the sample, which is held in the sample rack 11 on the sampler unit 12 and determined to be abnormal by the determination unit 60, is moved upward.

  The analysis control unit 45 controls the drive unit 41 based on input information such as analysis parameters of each inspection item, sample ID of each sample, and inspection items input from the operation unit 90. Then, a drive pulse is supplied to a stepping motor provided in each moving mechanism such as the first to third moving mechanisms 42 to 44 of the driving unit 41. And each of the sample container 10, sample rack 11, reagent container 13, reagent container 16, reaction container 19, sample dispensing probe 21, first reagent dispensing probe 24, second reagent dispensing probe 26, etc. of the analysis unit 40 Move the analysis unit.

  The detection unit 50 includes a liquid level detector 51 that detects contact and separation between the sample in the sample container 10 held in the sample rack 11 of the analysis unit 40 and the sample dispensing probe 21. In addition, a position detector 52 that detects the position of the sample rack 11 on the sample dispensing probe 21 and the sampler unit 12 and a pressure detector 53 that detects the pressure in the sample dispensing probe 21 are provided.

  As shown in FIG. 3A, the liquid level detector 51 has a dispensing position where the sample dispensing probe 21 moved downward from the upper stop position P1 above the dispensing position T1 is held by the sample rack 11. The contact between the sample dispensing probe 21 and the sample is detected by, for example, a change in capacitance when contacting the sample in the sample container 10 at T1.

  Further, when the sample is aspirated by the sample dispensing probe 21 after contact with the sample is detected and the sample in the sample container 10 runs short on the way, the sample dispensing is performed as shown in FIG. The separation between the sample dispensing probe 21 and the sample is detected by a change in capacitance when the probe 21 and the sample in the sample container 10 are separated.

  The position detector 52 detects the positions of the sample dispensing probe 21 and the sample rack 11 based on the number of drive pulses supplied from the analysis control unit 45 to the drive unit 41. Then, as shown in FIG. 3A, a distance D1 that the sample dispensing probe 21 has moved from the upper stop position P1 to a contact position P2 where contact with the sample in the sample container 10 is detected is calculated. Thereby, the position of the sample dispensing probe 21 in the contact position P2 is detected.

  The pressure detector 53 detects the pressure in the sample dispensing probe 21 when the sample is being dispensed. Then, when the sample in the sample container 10 is being sucked at the dispensing position T1, the suction pressure in the sample dispensing probe 21 is detected. Further, when the sample is being discharged into the reaction container 19, the discharge pressure in the sample dispensing probe 21 is detected. Furthermore, when the cleaning liquid is supplied to the sample dispensing probe 21 by the dispensing unit 23, the cleaning pressure in the sample dispensing probe 21 is detected.

  The determination unit 60 includes information on contact or separation between the sample dispensing probe 21 and the sample detected by the liquid level detector 51 of the detection unit 50, position information on the sample dispensing probe 21 detected by the position detector 52, and Based on the pressure information with respect to the atmospheric pressure in the sample dispensing probe 21 detected by the pressure detector 53, it is determined whether or not the sample dispensing by the sample dispensing probe 21 is normal.

  As shown in FIG. 3A, when the position of the sample dispensing probe 21 when contact with the sample is detected is higher than a preset lower limit position, the sample is placed in the sample container 10. Therefore, it is determined that the sample is dispensed normally. In addition, when the position of the sample dispensing probe 21 when contact with the sample is detected is lower than the lower limit position, the sample in the sample container 10 is insufficient, so that the sample dispensing is abnormal. It is determined that As shown in FIG. 4, when the separation between the sample dispensing lobe 21 and the sample is detected at the position P3 lower than the lower limit position, the sample in the sample container 10 is insufficient, so that the sample is dispensed. Is determined to be abnormal.

  Further, when the sample is being aspirated, when contact between the sample dispensing probe 21 and the sample is detected, and when the aspiration pressure is a pressure within a preset aspiration tolerance range lower than the atmospheric pressure Then, it is determined that the suction in the dispensing of the sample is normal. In addition, when the sample is being sucked, if the separation between the sample dispensing probe 21 and the sample is detected, the sample in the sample container 10 has run out in the middle, so the suction in dispensing the sample is abnormal. Is determined.

  Further, when the sample is being sucked, if the suction pressure is a pressure outside the allowable suction range, it is determined that the sample dispensing is abnormal. Here, when the suction pressure is lower than the lower limit of the suction permissible range, the sample dispensing probe 21 is clogged with insoluble matter such as fibrin, and therefore it is determined that the suction in dispensing the sample is abnormal. To do. In addition, when the suction pressure is higher than the upper limit pressure of the suction allowable range, it is determined that the suction in dispensing the sample is abnormal because bubbles are generated on the sample liquid surface in the sample container 10. .

  Further, when the sample is being discharged, it is determined that the discharge in the dispensing of the sample is normal when the discharge pressure is equal to or lower than a preset discharge upper limit pressure higher than the atmospheric pressure. Further, when the discharge pressure is higher than the discharge upper limit pressure, the sample dispensing probe 21 is clogged, and hence it is determined that the discharge during sample dispensing is abnormal.

  The determination unit 60 also dispenses the sample based on the cleaning pressure for the atmospheric pressure in the sample dispensing probe 21 detected by the pressure detector 53 when the cleaning liquid is supplied to the sample dispensing probe 21. It is determined whether or not the sample 21 can be continuously dispensed by the probe 21. When the cleaning pressure is a pressure equal to or lower than a preset cleaning upper limit pressure higher than the atmospheric pressure, it is determined that the sample dispensing probe 21 is not clogged, so that the sample can be continuously dispensed. When the cleaning pressure is higher than the cleaning upper limit pressure, it is determined that the sample dispensing probe 21 is unable to dispense the sample because the sample dispensing probe 21 is clogged.

  The data processing unit 70 processes the standard data and test data generated by the measurement unit 28 of the analysis unit 40 to generate calibration data and analysis data for each inspection item, and is generated by the calculation unit 71. And a data storage unit 72 for storing standard data and analysis data.

  The calculation unit 71 generates calibration data indicating the relationship between the standard data generated by the measurement unit 28 and a standard value preset in the standard sample, outputs the generated calibration data to the output unit 80 and the data storage unit 72. Save to. Also, calibration data corresponding to the test data generated by the measurement unit 28 is read from the data storage unit 72 to generate analysis data expressed as a concentration value or an enzyme activity value. The generated analysis data is output to the output unit 80 and stored in the data storage unit 72.

  The data storage unit 72 includes a memory device such as a hard disk, and stores the calibration data output from the calculation unit 71 for each inspection item. The analysis data of each inspection item output from the calculation unit 71 is stored for each sample.

  The output unit 80 includes a printing unit 81 that prints and outputs calibration data and analysis data output from the calculation unit 71 of the data processing unit 70 and a display unit 82 that displays and outputs the data. The printing unit 81 includes a printer or the like, and prints the calibration data and analysis data output from the calculation unit 71 on printer paper or the like according to a preset format.

  The display unit 82 includes a monitor such as a CRT or a liquid crystal panel, and displays calibration data and analysis data output from the calculation unit 71. In addition, an analysis parameter setting screen for setting analysis parameters such as the amount (dispensing amount) of the sample to be dispensed for each inspection item, the sample ID of this sample and the sample ID for each sample to be inspected Displays an inspection item setting screen for setting items.

  The operation unit 90 includes input devices such as a keyboard, a mouse, a button, and a touch key panel, and inputs for setting analysis parameters for each inspection item, a sample ID to be inspected, and a test set for the sample ID. Enter the item information.

  The system control unit 91 includes a CPU and a storage circuit, stores input information such as analysis parameters, sample IDs, and inspection items of each inspection item input from the operation unit 90 in the storage circuit, and then based on the input information. The analysis control unit 45, the detection unit 50, the determination unit 60, the data processing unit 70, and the output unit 80 are integrated to control the entire system.

  Next, the configuration and operation of the sampler unit 12, the sample container 10, the sample rack 11, the sample dispensing probe 21, the dispensing unit 23, and the pressure detector 53 of the detection unit 50 related to the sample dispensing. Details will be described.

  FIG. 5 is a top view showing the configuration of the sampler unit 12 and the sample dispensing probe 21. The sampler unit 12 includes a table 121 on which the sample rack 11 is placed and supports the placed sample rack 11. Further, a reader 122 that reads the rack ID written on the sample rack 11 and a reader 123 that reads the sample ID written on the sample container 10 are provided near the table 121.

  The table 121 is divided into a standby area E1, a pull-in area E2, a reading area E3, a dispensing area E4, a pull-out area E5, and a pick-up area E6. Then, the sample rack 11 that holds the sample container 10 containing the sample to be inspected is placed in the standby area E1. The sample rack 11 in the standby area E1 is moved in the L1 direction by the first moving mechanism 42 of the drive unit 41, and stops in the pull-in area E2. The sample rack 11 in the pull-in area E2 is moved in the L2 direction by the second moving mechanism 43 of the drive unit 41, and stops in the reading area E3.

  The sample rack 11 stopped in the reading area E3 is moved in the L1 direction by the second moving mechanism 43 and stopped in the dispensing area E4. The sample rack 11 stopped in the dispensing area E4 is moved in the L3 direction by the second moving mechanism 43, and stopped at the dispensing position T1 where the sample in the sample container 10 can be sucked by the sample dispensing probe 21. When the dispensing of the samples in all the sample containers 10 to be inspected is completed, the sample rack 11 is moved to the extraction area E5 by the second moving mechanism 43. The sample rack 11 moved to the extraction area E5 is moved in the L1 direction by the first movement mechanism 42 and stops in the extraction area E6.

  The reader 122 performs wireless communication with, for example, an IC tag on which a rack ID arranged on the sample rack 11 is written. Then, the rack ID of the sample rack 11 stopped in the pull-in area E2 of the table 121 is read and output to the analysis control unit 45. In addition, when the first moving mechanism 42 has moved the sample rack 11 by one in the L1 direction and cannot be read, the unreadable information indicating that there is no sample rack 11 in the area E2. Is output to the analysis control unit 45.

  The reader 123 performs wireless communication with, for example, an IC tag on which a sample ID placed in the sample container 10 is written. Then, after the rack ID is read by the reader 122, the sample ID of the sample container 10 at the ID reading position, which is held in the sample rack 11 moving from the pull-in area E2 to the reading area E3, is read. In addition, if it is impossible to read after the movement of the sample container 10 at the ID reading position by one in the L2 direction, it is confirmed that there is no sample container 10 held on the sample rack 11 at the ID reading position. The unreadable information shown is output to the analysis control unit 45.

  The analysis control unit 45 controls sample dispensing based on the sample ID and inspection item information input from the operation unit 90 and the rack ID and sample ID read by the readers 122 and 123. Then, among the sample containers 10 held in the sample rack 11 from which the rack ID and the sample ID are read, the sample ID in the sample container 10 corresponding to the sample ID input from the operation unit 90 is used as an inspection target, and the sample ID Are dispensed for each inspection item set in advance.

  FIG. 6 is a diagram showing the configuration of the sample rack 11. 6A shows an external view of the sample rack 11, and FIG. 6B shows a cross-sectional view taken along line AA in FIG. 6A. The sample rack 11 includes, for example, five through holes 11a for arranging five sample containers 10 in a row and holding them in a detachable manner. In addition, a holding body 11b such as a spring disposed in each through hole 11a that holds the sample container 10 inserted into each through hole 11a at a position moved upward by the third moving mechanism 44 of the drive unit 41. I have.

  FIG. 7 is a view showing the sample container 10 at the dispensing position T1 and the sample rack 11 on the sampler unit 12 that holds the sample container 10. An opening 12a is provided in the sampler portion 12 below the sample container 10 at the dispensing position T1. Then, as shown in FIG. 7A, the sample rack 11 holds the sample container 10 inserted up to a position where it is stopped by the operator at the reference position SL. Further, as shown in FIG. 7B, the sample container 10 is held at the abnormal position SH moved upward from the reference position SL by the third moving mechanism 44 of the driving unit 41.

  The third moving mechanism 44 has an arm 44a that moves the sample container 10 containing the sample that has been determined to be abnormal by the determination unit 60 at the dispensing position T1 held in the sample rack 11 upward. I have. Then, as shown in FIG. 7A, the arm 44a enters the through hole 11a from below the sampler portion 12 through the opening portion 12a, pushes up the sample container 10 from the reference position SL, and leads the sampler portion 12 to the reader. 123 moves upward to an abnormal position SH at which the sample ID cannot be read.

  Thus, the sample container 10 pushed up from the reference position SL by the third moving mechanism 44 is held in the sample rack 11 at the abnormal position SH where the sample ID written on the sample container 10 cannot be read by the reader 123. Can be made. This makes it impossible to read the sample ID from the sample container 10 at the abnormal position SH held in the sample rack 11, and thus dispensing of the sample in the sample container 10 at the abnormal position SH can be prevented.

  The sample dispensing probe 21 shown in FIG. 5 moves horizontally in the directions of the horizontal arrows R1 and R2 along the circular orbit indicated by the broken line, and stops at the upper stop position P1 shown in FIG. Then move down. Then, as shown in FIG. 8, the liquid level detector 51 stops at a suction stop position P4 moved downward by a distance D2 from a position where contact with the sample in the sample container 10 at the dispensing position T1 is detected. Next, after aspirating a sample of an amount corresponding to a predetermined dispensing amount of the inspection item from the sample container 10 at the dispensing position T1, a dispensing amount of the sample is discharged into the reaction container 19 stopped at the discharge position T2. To do. Then, when dispensing of all the inspection items set in advance on the sample in the sample container 10 at the dispensing position T1 is completed, the process moves to the cleaning position T3. Then, cleaning is performed at the cleaning position T3.

  Thus, by stopping at the suction stop position P4, it is possible to avoid contact with an unnecessary sample and reduce contamination of the outer wall of the sample dispensing probe 21 by the sample.

FIG. 9 is a diagram showing the configuration of the dispensing unit 23 and the pressure detector 53.
The dispensing unit 23 includes a tube 231 having one end connected to the sample dispensing probe 21, a syringe 232 connected to the other end of the tube 231, and a plunger that fits into an opening provided at the lower end of the syringe 232. 233. Moreover, the tank 234 which stores the pressure transmission medium with which each inside of the sample dispensing probe 21, the tube 231, and the syringe 232 is filled is provided.

  In addition, the dispensing unit 23 sucks the pressure transmission medium stored in the tank 234, and supplies the suctioned pressure transmission medium as a cleaning liquid into the sample dispensing probe 21 via the syringe 232 and the tube 231. It has. Further, an open / close valve 236 for opening and closing a flow path communicating between the syringe 232 and the cleaning pump 235 is provided.

  In the sample dispensing, the drive unit 41 sucks the plunger 233 in the direction of the arrow L4 in a state where the flow path between the syringe 232 and the cleaning pump 235 is closed by the on-off valve 236 driven and controlled by the analysis control unit 45. By driving, the sample dispensing probe 21 sucks the sample in the sample container 10 at the dispensing position T1. Further, when the drive unit 41 drives the plunger 233 to discharge in the direction of the arrow L5, the sample dispensing probe 21 discharges the sample into the reaction container 19 stopped at the discharge position T2.

  When the dispensing of the same sample is completed or when the determination unit 60 determines that the sample is dispensed abnormally, the cleaning pump 235 causes the syringe 232 and the cleaning pump 235 to be driven by the cleaning drive of the driving unit 41. The cleaning liquid is supplied into the sample dispensing probe 21 in a state where the flow path therebetween is opened by the on-off valve 236.

  One end of the pressure detector 53 is connected to the tube 231 of the dispensing unit 23 and includes a pressure sensor 531 that measures the pressure in the sample dispensing probe 21 with reference to the atmospheric pressure. In addition, an amplifier 532 that amplifies the signal of the pressure sensor 531 and an A / D converter 533 that converts the signal amplified by the amplifier 532 into a digital signal are provided.

  Then, a suction pressure lower than the atmospheric pressure in the sample dispensing probe 21 when the sample is sucked by the sample dispensing probe 21, that is, when a suction operation is performed by the dispensing unit 23 is detected. . Further, a discharge pressure higher than the atmospheric pressure in the sample dispensing probe 21 when the sample dispensing probe 21 is ejecting the sample, that is, when the dispensing operation is performed by the dispensing unit 23 is detected. . Further, a cleaning pressure higher than the atmospheric pressure in the sample dispensing probe 21 when the cleaning liquid supply operation is performed by the cleaning pump 235 of the dispensing unit 23 is detected.

  Hereinafter, an example of an operation related to sample dispensing in the automatic analyzer 100 will be described with reference to FIGS. 1 to 12.

FIGS. 10 to 12 are flowcharts showing operations related to sample dispensing of the automatic analyzer 100.
After the sample ID and the information of the inspection item set for the sample ID are input from the operation unit 90, the sample rack 11 that holds the sample container 10 that stores the sample is on standby on the table 121 in the sampler unit 12. Placed in the area E1. Then, when an inspection start input is made from the operation unit 90, the automatic analyzer 100 starts operation (step S1 in FIG. 10).

  The system control unit 91 instructs the analysis control unit 45, the detection unit 50, and the determination unit 60 to dispense the sample to be inspected based on the input information input from the operation unit 90. The first moving mechanism 42 of the drive unit 41 moves the sample rack 11 placed in the standby area E1 to the pull-in area E2. The reader 122 reads the rack ID of the sample rack 11 in the pull-in area E2. The second moving mechanism 43 moves the sample rack 11 from which the rack ID has been read to the reading area E3.

  The reader 123 reads the sample ID of the sample container 10 at the ID reading position held by the sample rack 11 moving from the pull-in area E2 to the reading area E3. The second moving mechanism 43 further moves the sample rack 11 moved to the reading area E3 to the dispensing area E4, and then dispenses the sample container 10 containing the sample to be inspected whose sample ID is read by the reader 123. The sample rack 11 is stopped at a position that reaches the position T1.

  For example, the sample dispensing probe 21 moves from the cleaning position T3 to the upper stop position P1 of the dispensing position T1, and then moves downward from the upper stop position P1. The liquid level detector 51 detects contact between the sample in the sample container 10 stopped at the dispensing position T1 and the sample dispensing probe 21. The position detector 52 detects the position of the sample dispensing probe 21 when contact with the sample is detected (step S2 in FIG. 10).

  The determination unit 60 determines whether or not the sample dispensing is normal based on the position information of the sample dispensing probe 21 when contact with the sample is detected. When the position of the sample dispensing probe 21 is higher than the lower limit position (Yes in step S3 in FIG. 10), it is determined that the sample dispensing is normal. When the position of the sample dispensing probe 21 is lower than the lower limit position (No in step S3 in FIG. 10), it is determined that the sample dispensing is abnormal. And it transfers to step S20 of FIG.

  After “Yes” in step S3 in FIG. 10, the sample dispensing probe 21 aspirates a sample of an amount corresponding to a predetermined dispensing amount of the inspection item from the sample container 10 at the dispensing position T1. The liquid level detector 51 detects contact or separation between the sample dispensing probe 21 and the sample when the sample is sucked (step S4 in FIG. 10).

  The determination unit 60 determines based on contact or separation detection information of the liquid level detector 51. If the separation between the sample dispensing probe 21 and the sample is detected (No in step S5 in FIG. 10), it is determined that the sample dispensing is abnormal, and the process proceeds to step S20 in FIG.

  After “Yes” in step S3 in FIG. 10, the sample dispensing probe 21 aspirates a sample of an amount corresponding to a predetermined dispensing amount of the inspection item from the sample container 10 at the dispensing position T1. The pressure detector 53 detects the suction pressure in the sample dispensing probe 21 when the sample is being sucked (step S6 in FIG. 10).

  The determination unit 60 determines based on the suction pressure detected by the pressure detector 53. If the suction pressure is outside the allowable suction range (No in step S7 in FIG. 10), it is determined that the sample dispensing is abnormal, and the process proceeds to step S20 in FIG.

  Moreover, the determination part 60 is the case where the contact with the sample dispensing probe 21 and the sample is detected (Yes in step S5 in FIG. 10) and the suction pressure is within the suction allowable range (in step S7 in FIG. 10). Yes), determine that sample dispensing is normal.

  After “Yes” in step S5 and “Yes” in step S7 in FIG. 10, the sample dispensing probe 21 moves and discharges a predetermined amount of sample into the reaction container 19 stopped at the discharge position T2. To do. The pressure detector 53 detects the discharge pressure in the sample dispensing probe 21 when the sample is being discharged (step S8 in FIG. 10).

  The determination unit 60 determines based on the discharge pressure detected by the pressure detector 53. When the discharge pressure is equal to or lower than the discharge upper limit pressure (Yes in step S9 in FIG. 10), it is determined that the sample is dispensed normally. And it transfers to step S10 of FIG. If the discharge pressure is higher than the discharge upper limit pressure (No in step S9 in FIG. 10), it is determined that the sample dispensing is abnormal. And it transfers to step S20 of FIG.

  After “No” in Step S3, “No” in Step S5, “No” in Step S7, or “No” in Step S9 in FIG. 10, the analysis control unit 45 determines whether the determination unit 60 determines abnormality. The determination information, the sample ID of the sample to be re-inspected that has been determined to be abnormal, the inspection item when the sample is determined to be abnormal, and the inspection item that has not been inspected among the inspection items set for the sample ID Dispensing stop information including information on the inspection item to be inspected and the rack ID of the sample rack 11 holding the sample container 10 of the sample ID is stored. Then, the dispensing operation of the sample dispensing probe 21 is stopped. In addition, the system control unit 91 causes the display unit 82 of the output unit 80 to display and output the dispensing stop information according to the determination result of the determination unit 60.

  The sample dispensing probe 21 stops dispensing the sample that has been determined to be abnormal in the sample container 10 at the dispensing position T1. The third moving mechanism 44 moves the sample container 10 at the dispensing position T1 containing the sample determined to be abnormal from the reference position SL to the abnormal position SH upward (step S20 in FIG. 11). .

  Thus, by moving the sample container 10 that holds the sample that is held in the sample rack 11 and is determined to be abnormal in dispensing to the abnormal position SH, the sample container 10 is sampled at the abnormal position SH. The rack 11 can be held. Thereby, the sample container 10 which accommodates the sample determined to be abnormal can be easily found.

  The sample dispensing probe 21 moves to the cleaning position T3. The cleaning pump 235 of the dispensing unit 23 supplies the cleaning liquid into the sample dispensing probe 21. The pressure detector 53 detects the cleaning pressure in the sample dispensing probe 21 when the cleaning liquid supply operation is performed (step S21 in FIG. 11).

  The determination unit 60 determines based on the cleaning pressure detected by the pressure detector 53. If the cleaning pressure is equal to or lower than the cleaning upper limit pressure (Yes in step S22 in FIG. 11), it is determined that the sample can be dispensed continuously. And it transfers to step S10 of FIG. If the cleaning pressure is higher than the cleaning upper limit pressure (No in step S22 in FIG. 11), it is determined that the sample cannot be dispensed.

  The analysis control unit 45 determines the sample ID of the sample that has been dispensed immediately before the determination information cleaning and the sample rack that holds the sample container 10 of this sample ID according to the determination that the determination unit 60 cannot dispense. Dispensable information including information of 11 rack IDs is stored. Then, the dispensing operation of the sample dispensing probe 21 is stopped. The system control unit 91 causes the display unit 82 to display and output non-dispensable information in accordance with the non-dispensable determination of the determination unit 60. Thereafter, the process proceeds to step S14 in FIG.

  When “Yes” in step S9 in FIG. 10 or “Yes” in step S22 in FIG. 11, when the dispensing of the sample in the sample container 10 at the dispensing position T1 ends (Yes in step S10 in FIG. 12). The process proceeds to step S11 in FIG. Further, when the dispensing of the sample in the sample container 10 at the dispensing position T1 continues (No in step S10 in FIG. 12), the process returns to step S2 in FIG.

  When an unexamined sample container 10 among the sample containers 10 to be inspected is held in the sample rack 11 holding the sample container 10 at the dispensing position T1 (Yes in step S11 in FIG. 12), the second movement The mechanism 43 further moves the sample rack 11 in the L3 direction, and stops the uninspected sample container 10 at a position where it reaches the dispensing position T1. Thereafter, the process returns to step S2. Further, when the unchecked sample container 10 is not held in the sample rack 11 holding the sample container 10 at the dispensing position T1 (No in step S11 in FIG. 12), the process proceeds to step S12 in FIG.

  After “No” in step S11 in FIG. 12, the second moving mechanism 43 moves the sample rack 11 holding the sample container 10 at the dispensing position T1 to the drawer area E5. The first moving mechanism 42 moves the sample rack 11 in the pull-out area E5 to the take-out area E6 (Step S12 in FIG. 12).

  Thus, the sample rack 11 holding the sample container 10 at the abnormal position SH can be moved to the take-out area E6 where the take-out is easy. Thereby, the sample container 10 that accommodates the sample determined to be abnormal can be easily found and can be easily taken out from the sample rack 11.

  After step S12 in FIG. 12, when there is a sample rack 11 holding the sample container 10 to be inspected whose sample ID is read by the reader 123 (Yes in step S13 in FIG. 12), the second moving mechanism 43 is The sample rack 11 is moved to a position where the sample container 10 to be inspected reaches the dispensing position T1. Then, it returns to step S2 of FIG. If there is no sample rack 11 that holds the sample container 10 to be inspected whose sample ID is read by the reader 123 (No in step S13 in FIG. 12), the process proceeds to step S14 in FIG.

  After “No” in step S22 in FIG. 11 or “No” in step S13 in FIG. 12, the automatic analyzer 100 ends the sample dispensing operation in response to the input of the examination start from the operation unit 90 (FIG. 11). 12 step S14).

  After step S14 in FIG. 12, when there is the sample rack 11 stopped at the abnormal position SH in the take-out area E6, the dispensing stop information corresponding to the sample container 10 at the abnormal position SH or the dispensing abnormality information of the non-dispensable information. Is displayed on the display unit 82.

  Here, when the number of samples in the sample container 10 at the abnormal position SH is small, for example, reexamination treatment for retesting such as replenishment or replacement with the sample container 10 containing the sample is performed. Further, when clogging occurs, the insoluble matter in the sample container 10 and the insoluble matter blocking the sample dispensing probe 21 are removed, and retesting is performed. Then, after placing the sample rack 11 into which the re-examined sample container 10 is inserted to the original reference position SL in the waiting area E1, when the re-examination start is input from the operation unit 90, the automatic analyzer 100 performs the re-examination sample. The dispensing operation is started.

  As described above, the sample container 10 containing the sample determined to be abnormal in dispensing can be easily found out and can be easily taken out from the sample rack 11, so that the retest is executed. Preparatory work can be done quickly. Then, by inserting the reexamined sample container 10 to the original reference position SL of the sample rack 11, the reader 123 can read the sample ID and perform reexamination.

  Based on the dispensing abnormality information during the inspection from the start of the inspection to the end of the sample dispensing operation, and the rack ID and the sample ID read by the readers 122 and 123 during the retest, the analysis control unit 45 performs the retest target. The test items to be retested in the sample are dispensed.

  Here, among the rack IDs read during the reexamination, the sample rack 11 that matches the rack ID included in the dispensing abnormality information stored during the inspection in the analysis control unit 45 becomes the reexamination target. In addition, among the sample IDs read during the reexamination, the sample container 10 held in the reexamination target sample rack 11 that matches the sample ID included in the dispensing abnormality information saved during the inspection becomes the reexamination target. . Then, the sample of the inspection item to be retested is dispensed from the sample container 10 to be retested according to steps S2 to S13 in FIGS. 10 and 11 and steps S20 to S22 in FIG.

  In this way, the sample rack 11 into which the sample container 10 containing the retested sample is inserted up to the reference position SL is placed in the standby area E1, and the retest is performed by simply performing the retest start input from the operation unit 90. Of the target samples, the sample of the inspection item to be retested can be dispensed and retested.

  According to the embodiment described above, the position of the sample dispensing probe 21 when it comes into contact with the sample in the sample container 10 held in the sample rack 11 can be detected. When the position of the sample dispensing probe 21 is higher than the lower limit position, it can be determined that the sample dispensing is normal. Moreover, when the position of the sample dispensing probe 21 is lower than the lower limit position, it can be determined that the sample dispensing is abnormal.

  Further, when the sample is sucked, the contact or separation between the sample dispensing probe 21 and the sample and the suction pressure in the sample dispensing probe 21 can be detected. When the contact between the sample dispensing probe 21 and the sample is detected and when the suction pressure is within the allowable range, it can be determined that the sample dispensing is normal. Further, when the separation between the sample dispensing probe 21 and the sample is detected or when the suction pressure is out of the suction allowable range, it can be determined that the sample dispensing is abnormal.

  Moreover, when the sample is being discharged, the suction pressure in the sample dispensing probe 21 can be detected. When the discharge pressure is equal to or lower than the discharge upper limit pressure, it can be determined that the sample is dispensed normally. Further, when the discharge pressure is higher than the discharge upper limit pressure, it can be determined that the sample is dispensed abnormally.

  Then, the sample container 10 containing the sample determined to be abnormal is moved upward to the abnormal position SH, and the sample rack 11 holding the sample container 10 at the abnormal position SH can be easily removed. You can move up to E6. As a result, the sample container 10 that accommodates the sample determined to be abnormal in dispensing can be easily found out and can be easily taken out from the sample rack 11, thereby reducing the work of retesting. Can do.

  Although several embodiments of the present invention have been described, these embodiments are presented by way of example and are not intended to limit the scope of the invention. These novel embodiments can be implemented in various other forms, and various omissions, replacements, and changes can be made without departing from the scope of the invention. These embodiments and modifications thereof are included in the scope and gist of the invention, and are included in the invention described in the claims and the equivalents thereof.

SL Reference position SH Abnormal position 10 Sample container 11 Sample rack 21 Sample dispensing probe 44 Third moving mechanism 51 Liquid level detector 52 Position detector 53 Pressure detector 60 Determination unit 122, 123 Reader

Claims (10)

In an automatic analyzer that dispenses the sample and reagent in the sample container into the reaction container and measures the mixture,
A sample dispensing probe for performing dispensing to suck the sample in the sample container and discharge it to the reaction container;
A pressure detector for detecting the pressure in the sample dispensing probe when the sample is being dispensed;
A determination unit for determining whether or not the sample dispensing by the sample dispensing probe is normal based on the pressure detected by the pressure detector;
A sample rack for holding the sample container;
A moving mechanism that moves the sample container that holds the sample that is determined to be abnormal by the determination unit as the sample container held in the sample rack;
The automatic analyzer according to claim 1, wherein the sample rack holds the sample container at a position moved upward by the moving mechanism. The moving mechanism has a container moving mechanism that moves the sample container upward and a rack moving mechanism that horizontally moves the sample rack,
The rack moving mechanism stops the sample rack at a dispensing position where the sample in the sample container held by the sample rack can be sucked by the sample dispensing probe,
The automatic analyzer according to claim 1, wherein the container moving mechanism moves the sample container at the dispensing position held in the sample rack upward. A reader that reads a sample ID written on the sample container at a position lower than the position moved upward by the moving mechanism;
The automatic analyzer according to claim 1 or 2, wherein the position moved upward by the moving mechanism is a height at which the sample ID cannot be read by the reader. In an automatic analyzer that dispenses the sample and reagent in the sample container into the reaction container and measures the mixture,
A sample dispensing probe for performing dispensing to suck the sample in the sample container and discharge it to the reaction container;
A pressure detector for detecting the pressure in the sample dispensing probe when the sample is being dispensed;
A determination unit for determining whether or not the sample dispensing by the sample dispensing probe is normal based on the pressure detected by the pressure detector;
A moving mechanism for moving upward the sample container containing the sample determined to be abnormal by the determination unit;
A reader for reading a sample ID written on the sample container other than the sample container moved upward by the moving mechanism, the position being lower than the position moved upward by the moving mechanism; Prepared,
The automatic analyzer is characterized in that the position moved upward by the moving mechanism is a height at which the sample ID cannot be read by the reader. A sample rack for holding the sample container;
The automatic analyzer according to claim 4, wherein the moving mechanism moves the sample container held by the sample rack upward. The moving mechanism has a container moving mechanism that moves the sample container upward and a rack moving mechanism that horizontally moves the sample rack,
The rack moving mechanism stops the sample rack at a dispensing position where the sample in the sample container held by the sample rack can be sucked by the sample dispensing probe,
6. The automatic analyzer according to claim 5, wherein the container moving mechanism moves the sample container at the dispensing position held by the sample rack upward. A liquid level detector for detecting contact or separation between the sample in the sample container and the sample dispensing probe, and a position detector for detecting the position of the sample dispensing probe;
The determination unit
When the position of the sample dispensing probe when contact with the sample is detected is higher than a preset lower limit position, it is determined to be normal,
When the position of the sample dispensing probe when contact with the sample is detected is lower than the lower limit position, or between the sample dispensing probe and the sample at a position lower than the lower limit position The automatic analyzer according to claim 1, wherein when the separation is detected, it is determined that there is an abnormality. The sample dispensing probe sucks the sample in the sample container when the position of the sample dispensing probe when contact with the sample is detected is higher than the lower limit position,
The determination unit
When the sample is sucked, the suction pressure detected by the pressure detector is a pressure within a preset suction tolerance range lower than atmospheric pressure, and the sample, the sample dispensing probe, If contact is detected, it is determined to be normal,
When the sample is being suctioned, if the suction pressure is outside the allowable suction range or if a separation between the sample and the sample dispensing probe is detected, it is determined as abnormal. The automatic analyzer according to claim 7. When it is determined that the sample dispensing probe is normal when the sample is being aspirated, the sample is dispensed into the reaction container,
The determination unit
When the sample is being discharged, if the discharge pressure detected by the pressure detector is a pressure equal to or lower than a preset discharge upper limit pressure higher than atmospheric pressure, it is determined to be normal,
9. The automatic analyzer according to claim 8, wherein when the discharge pressure is higher than the discharge upper limit pressure, it is determined that there is an abnormality. When dispensing of the sample in the sample container is completed, or when determined to be abnormal by the determination unit, a supply pump that supplies a cleaning liquid into the sample dispensing probe,
The determination unit
When the cleaning pressure detected by the pressure detector when the cleaning liquid is being supplied is equal to or lower than a preset supply upper limit pressure that is higher than atmospheric pressure, the sample dispensing probe continuously Determine that dispensing is possible,
10. The method according to claim 1, wherein when the cleaning pressure is higher than the supply upper limit pressure, it is determined that the sample cannot be dispensed by the sample dispensing probe. The automatic analyzer described.

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