JP2011227092A - Automatic analyzing apparatus - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an automatic analyzing apparatus with which influence among test samples can be reduced and the test samples can be dispensed with accuracy.SOLUTION: In the automatic analyzing apparatus in which test samples and reagent corresponding to measurement items of the test samples are dispensed into a reaction vessel and the mixture thereof is measured, reaction vessels 4A and 4B respectively corresponding to A and B measurement lines are arranged in plural on periphery and moved for each analyzing cycle. The automatic analyzing apparatus further includes a reaction disc 5 stopping, a dispensing probe 16 for discharging the test samples from a sample container 17 into the reaction vessels 4A and 4B for each analyzing cycle, a sample dispensing arm 10, and a sample dispensing pump 10a. The test samples to be discharged into the reaction vessel 4A are taken up from the sample container 17 during movement of the reaction disc 5. The test samples are discharged into the reaction vessel 4A during halts after movement of the reaction disc 5. Then, further test samples are taken up from the sample container 17 and discharged into the reaction vessel 4B.

Description

The present invention relates to an automatic analyzer for analyzing components contained in a liquid and a method for dispensing the same, and in particular, dispenses bodily fluids such as human blood and urine to automatically remove the components contained in the body fluids. The present invention relates to an automatic analyzer for measuring.

The automatic analyzer measures the amount of light transmitted through changes in the color tone caused by the reaction of the mixture of the test sample dispensed into the reaction container and the reagent corresponding to the measurement item such as biochemistry or immunity to be analyzed. Thus, the concentration of various substances to be measured in the test sample and the activity of the enzyme are measured.

In this automatic analyzer, the amount of test sample and reagent used for measurement, the reaction time of the mixture,
Measurement conditions selected for each of a large number of test samples by setting analysis conditions such as the wavelength of light in advance for each measurement item and selecting and setting measurement items for each test sample before starting the measurement. Items can be measured. Then, an automatic analyzer that performs measurement by a random access method according to the number of subjects × measurement items and a semi-random access method automatic analyzer that can perform higher-speed processing than the random access method are known ( For example, see Patent Document 1.)

In the random access method, a test sample is dispensed into one reaction vessel using one sample dispensing probe corresponding to one measurement line for each analysis cycle. Then, the first reagent or the first and second reagents corresponding to the measurement item selected and set in advance using the first reagent dispensing probe and the second reagent dispensing probe corresponding to the measurement line are detected. The sample is dispensed into a reaction vessel into which the sample has been dispensed.

In the semi-random access method, it was provided so that multiple measurement items could be measured.
Samples for multiple measurement items are aspirated at once using a sample dispensing probe that is smaller than the number of measurement lines corresponding to multiple measurement lines, and the same number of reaction vessels as the measurement lines corresponding to the measurement lines are collected. There is a multi-item dispensing method that discharges in multiple times. In addition, there is a single item dispensing method in which a test sample for one measurement item is sucked using the same number of sample dispensing probes as the measurement line and discharged to the same number of reaction containers as the measurement line corresponding to the measurement line.

And in these methods, the measurement vessel selected and set in advance using the same number of first reagent dispensing probes and second reagent dispensing probes as the number of measurement lines corresponding to the measurement line in the reaction container into which the test sample is dispensed. The first reagent or the first and second reagents corresponding to the same number of measurement items as the line are dispensed, and when the measurement is completed, the reaction container is washed and dried and used again for the measurement. In addition, the sample dispensing probe is washed every time after the dispensing of the same test sample is completed, and is used for dispensing the next test sample.

By the way, in the automatic analyzer, the test sample is aspirated and discharged from the sample dispensing probe via a pressure transmission medium such as water sealed in a tube between the sample dispensing pump and the sample dispensing probe that performs suction and discharge operations. Discharged. Then, since the tip of the test sample in the vicinity of the pressure transmission medium in the sample dispensing probe is diluted by the pressure transmission medium, the tip is not used for measurement. Treated as a dummy to prevent dilution of the test sample used for measurement.

JP-A-9-101313

However, in the semi-random access method of the single item dispensing method, since a plurality of sample dispensing probes enter the test sample in the sample container for aspiration, the samples are dispensed before through the plurality of sample dispensing probes. The test sample is brought into the sample container, and there is a problem that the test sample in which, for example, an extremely low concentration immunity item is measured is easily affected. In addition, the number of sample dispensing systems such as the sample dispensing probe, sample dispensing pump, and mechanism for driving the sample dispensing pump for dispensing the sample to be tested is the same as the number of measurement lines. There is a problem that becomes complicated and expensive.

On the other hand, in the multi-item dispensing method, many test samples are sucked into the sample dispensing probe at a time, so that not only the dummy part but also the test sample near the dummy is diluted and the sample dispensing accuracy decreases. As a result, there is a problem that the measurement accuracy decreases.

The present invention has been made to solve the above problems, and reduces the influence between test samples.
An object of the present invention is to provide an automatic analyzer capable of dispensing a test sample with high accuracy and a dispensing method thereof.

In order to achieve the above object, the automatic analyzer of the present invention according to claim 1 comprises a dispensing means for sucking a test sample from a sample container containing the test sample and discharging the sample into the reaction container, When the test sample is discharged into the reaction container by the first sample amount, the first suction time required when the test sample is sucked from the sample container by the first sample amount by the dispensing means. The first discharge time required, the second suction time required when the test sample is sucked from the sample container by the amount of the second sample smaller than the first sample amount, and the test sample in the reaction container Based on the second discharge time required for discharging the second sample amount, the difference between the time required for dispensing the first sample amount and the time required for dispensing the second sample amount A calculating means for calculating a certain surplus time, and based on the surplus time, Control means for changing the control of the dispensing means.

The figure which shows the structure of the automatic analyzer by the Example of this invention. The perspective view which shows the structure of the analysis part which concerns on the Example of this invention. The figure which shows a part of structure of the sample part and reaction part which concern on the Example of this invention. The flowchart which shows the sample dispensing process and sample dispensing probe washing | cleaning process which concern on the Example of this invention. The timing chart which shows the sample dispensing process and sample dispensing probe washing | cleaning process which concern on the Example of this invention. The flowchart which shows operation | movement of the automatic analyzer which concerns on the Example of this invention. The figure which shows an example of the measurement item setting screen which concerns on the Example of this invention. The figure which shows the sample dispensing process corresponding to each measurement item for every test sample selected and input from the measurement item setting screen which concerns on the Example of this invention. The timing chart which shows the sample dispensing process which concerns on the other Example of this invention. The timing chart which shows the sample dispensing process which concerns on the other Example of this invention. The timing chart which shows the sample dispensing B process which concerns on the Example of FIG. 10 of this invention. The timing chart which shows the sample dispensing process and sample dispensing probe washing | cleaning process which concern on the Example of FIG. 10 of this invention.

  Hereinafter, an embodiment of an automatic analyzer according to the present invention will be described with reference to FIGS.

FIG. 1 is a block diagram showing a configuration of an automatic analyzer according to an embodiment of the present invention. The automatic analyzer 100 includes a calibrator for various measurement items and an analysis unit 19 for measuring measurement items selected and input for each test sample, an analysis control unit 30 for controlling measurement operations of the analysis unit 19,
Analysis data processing unit 4 that processes the analysis signal output from the analysis unit 19 to generate analysis data
0, an output unit 50 for outputting analysis data from the analysis data processing unit 40, an operation unit 6 for setting analysis conditions for each measurement item, selecting and inputting measurement items for each test sample, and various command signals
0 and a system control unit 70 that controls these units in an integrated manner.

The analysis unit 19 relates to a calibrator for each measurement item, a sample unit 20 having an analysis unit related to a sample such as a test sample collected from a subject, and a reagent for causing a chemical reaction with a component of the measurement item of the sample. A reagent part 21 having an analysis unit and the like, and a reaction part 22 having an analysis unit for measuring a mixed solution of a sample and a reagent are provided. And
A calibrator signal, an analysis signal, and the like generated by measuring the calibrator and the test sample are output to the analysis data processing unit 40.

The analysis control unit 30 is a mechanism unit 3 having mechanisms for driving the analysis unit of the analysis unit 19 and the like.
1 and a control unit 32 that controls each mechanism of the mechanism unit 31.

The analysis data processing unit 40 creates a calibration table for each measurement item from the calibrator signal and analysis signal output from the analysis unit 19, calculates analysis data for each measurement item of each test sample, and the like. And a storage unit 42 in which a calibration table created by the calculation unit 41, calculated analysis data, and the like are stored.

The calculation unit 41 creates a calibration table for each measurement item from the calibrator signal for each measurement item output from the analysis unit 19, outputs the calibration table to the output unit 50, and saves it in the storage unit 42. In addition, for the analysis signal of each measurement item of each test sample output from the analysis unit 19,
After reading the calibration table of the measurement items from the storage unit 42, the analysis data is calculated using the calibration table and output to the output unit 50 and the storage unit 42.
Save to.

The storage unit 42 includes a hard disk and the like, and a calibration table, analysis data, and the like output from the calculation unit 41 are stored for each test sample.

The output unit 50 includes a printing unit 51 that prints and outputs a calibration table, analysis data, and the like output from the analysis data processing unit 40 and a display unit 52 that displays and outputs the calibration table.

The printing unit 51 includes a printer or the like, and prints out the calibration table, analysis data, and the like output from the analysis data processing unit 40 on printer paper or the like according to a preset format.

The display unit 52 includes a monitor such as a CRT or a liquid crystal panel, and includes a calibration table and analysis data output from the analysis data processing unit 40, a subject information input screen for inputting a subject ID and name, and the like for each measurement item. Display of an analysis condition setting screen for setting the analysis conditions, and a measurement item setting screen for selecting and setting measurement items for each test sample.

The operation unit 60 includes input devices such as a keyboard, a mouse, a button, and a touch key panel, sets analysis conditions for each measurement item, inputs subject information such as a subject ID and subject name of the subject, and subjects Various operations such as selection input of measurement items for each sample, calibration of each measurement item, and measurement of a test sample are performed.

The system control unit 70 includes a CPU and a storage circuit (not shown), and stores information such as an operator command signal, measurement item analysis conditions, subject information, and measurement items for each test sample supplied from the operation unit 60. After that, based on this information, control of the entire system such as control for causing each analysis unit constituting the analysis unit 19 to perform measurement operation in a predetermined sequence of a predetermined cycle, creation of a calibration table, calculation and output of analysis data, etc. I do.

Next, details of the configurations of the analysis unit 19 and the analysis control unit 30 will be described with reference to FIGS.

FIG. 2 is a perspective view for explaining the configuration of the sample unit 20, the reagent unit 21, and the reaction unit 22 of the analysis unit 19.

The sample unit 20 includes a sample container 17 containing samples such as a calibrator, a quality control sample, and a test sample, a sample container 17 set at a calibrator setting position, and a normal sample set at a quality control sample setting position. A sampler 6a that rotatably holds the container 17, a sample container 17 set at a test sample setting position, a micro sample container 17b that accommodates a micro test sample collected from a child or the like so as to be sucked, and the like. A rack 17a to be held and a plurality of racks 1
And a sampler 6b for holding 7a movably.

Further, the reaction container 4A at the sample discharge positions A and B corresponding to the A and B measurement lines provided so as to be able to suck a sample from the sample container 17 and measure a plurality of measurement items of the reaction unit 22
, 4B, and a sample dispensing probe 16 that is washed at the sample dispensing probe washing position every time the dispensing of the same sample is completed, and this sample dispensing probe 16 is moved to the calibrator suction position, the sample suction position for quality control, A sample dispensing arm 10 is provided which holds the specimen suction position, the sample discharge positions A and B, and the sample dispensing probe cleaning position so as to be rotatable and vertically movable at each position.

Note that the sample dispensing probe 16 enters the predetermined depth of about 2 mm from the liquid level of the test sample to suck the test sample and detects the liquid level. It is necessary to put the test sample so as to be deeper than the depth. And the normal sample container 17 can accommodate the test sample of the quantity which can measure many items. However, since a small amount of test sample can be collected from a child, the sample container 17b capable of aspirating a small amount of test sample can be used to store a test sample deeper than the predetermined depth. The horizontal cross section of the opening and the accommodating portion is smaller than that of the sample container 17.

FIG. 3 is a view of a part of the configuration of the sample unit 20 and the reaction unit 22 of the analysis unit 19 as viewed from above. The sample dispensing arm 10 has an arrow R1 with the rotation axis of the sample dispensing arm 10 as an axis.
And the sample dispensing probe 16 is moved along the movement locus of the sample dispensing probe 16 shown by a broken line in FIG.

Below the movement trajectory of the sample dispensing probe 16, the sample discharge positions A and B, the test sample suction position located in the vicinity of the sample discharge positions A and B, the sample discharge positions A and B, and the test sample There is a sample dispensing probe cleaning position located between the suction positions. The sample dispensing probe 16 is between the sample discharge positions A and B and the test sample suction position, between the sample discharge positions A and B and the sample dispensing probe cleaning position, and between the sample dispensing probe cleaning position and the test sample suction position. You can move between them in a short time.

The sample dispensing probe 16 is a rack 1 that holds a sample container 17 or the like containing a test sample.
7a moves in the direction of the arrow L1 and stops at the test sample suction position. For example, the test samples in the sample containers 17 of “1” to “5” are stopped at the sample discharge positions A and B. Dispense into. Then, the sample container 17 is washed at the sample dispensing probe washing position every time the sample dispensing of the sample container 17 is completed.

2 includes a reagent bottle 7a containing a first reagent that selectively reacts with a sample, a reagent bottle 7b containing a second reagent paired with the first reagent, and a first reagent. A reagent rack 1a for storing the reagent bottle 7a and a reagent store 2 for rotatably holding the reagent rack 1a
And a reagent rack 1b for storing the reagent bottle 7b containing the second reagent, and a reagent store 3 for rotatably holding the reagent rack 1b.

Further, the reagent unit 21 sucks the first reagent from the reagent bottle 7a at the 1A and 1B reagent suction positions (not shown), and the 1A and 1B (not shown) corresponding to the A and B measurement lines of the reaction unit 22 are drawn.
First reagent dispensing probes 14a and 14b that are ejected from the reagent ejection position and washed at the first A and 1B reagent dispensing probe washing positions (not shown) each time the first reagent dispensing is completed, and second A and 2B reagents (not shown) The second reagent is aspirated from the reagent bottle 7b at the aspiration position, discharged from the second A and second B reagent discharge positions (not shown) corresponding to the A and B measurement lines, and the second A (not shown) every time the second reagent dispensing ends. Second reagent dispensing probes 15a and 15b for cleaning at the second B reagent cleaning position are provided.

Further, the reagent unit 21 positions the first reagent dispensing probes 14a and 14b in the first A and first B reagent suction positions, the first A and first B reagent discharge positions, and the first A and first B reagent dispensing probe washing positions. The first reagent dispensing arms 8a and 8b and the second reagent dispensing probes 15a and 15b, which can be rotated between them and can be moved up and down at each position, and the second reagent dispensing probes 15a and 15b are moved to the second and second reagent aspirating positions, 2B
Second reagent dispensing arms 9a and 9b that hold the reagent discharge position and the second A and second B reagent dispensing probe cleaning positions so as to be rotatable and vertically movable at each position are provided.

The reaction unit 22 includes a sample dispensed from the sample dispensing probe 16 stopped at the sample ejection positions A and B, and a first reagent dispensing probe 14a stopped at the first A and 1B reagent ejection positions.
Corresponding to A and B measurement lines for receiving the first reagent dispensed from 14b and the second reagent dispensed from second reagent dispensing probes 15a and 15b stopped at the 2A and 2B reagent discharge positions The reaction vessels 4 of the reaction vessel group composed of the reaction vessels 4A and 4B, and the reaction disk 5 that rotatably holds a plurality of reaction vessels 4 arranged on the circumference are provided.

Then, the reaction disk 5 rotates counterclockwise in an analysis cycle of, for example, 4.5 seconds and stops, and rotates, for example, by an angle obtained by adding one round and the reaction vessels 4A and 4B in four analysis cycles. In addition, you may make it rotate clockwise. Moreover, you may make it rotate only the angle which subtracted 1 round and reaction container 4A, 4B part.

In addition, the reaction unit 22 agitates the mixed solution of the sample and the first reagent in the reaction vessel 4 stopped at the 1A and 1B stirring positions (not shown) corresponding to the A and B measurement lines, and the stirring of the mixed solution is completed. The 1A and 1B stirrers (not shown) that are cleaned at the 1A and 1B stirrer cleaning positions (not shown) are rotated between the 1A and 1B stirrer positions and the 1A and 1B stirrer cleaning positions, and A first agitation unit 11a is provided to hold the position so as to move up and down.

Further, the sample, the first reagent, and the second reagent in the reaction vessel 4 stopped at the 2A and 2B stirring positions (not shown) corresponding to the A and B measurement lines are stirred, and the stirring of the mixed liquid is completed. The 2A and 2B stirrers (not shown) that are cleaned at the 2A and 2B stirrer cleaning positions (not shown) are rotated between the 2A and 2B stirring positions and the 2A and 2B stirring and cleaning positions, and And a second agitation unit 11b that can be moved up and down at the position.

Further, the reaction unit 22 irradiates light when the reaction vessel 4 containing the mixed liquid after the first or second stirring passes the photometric position, measures the absorbance at the set wavelength from the transmitted light, and analyzes the signal. The photometric unit 13 that generates the liquid and the liquid mixture that has been measured in the reaction vessel 4 stopped at the washing and drying positions are sucked, and the washing nozzle for washing the reaction vessel 4 and the drying nozzle for drying can be moved up and down. And a cleaning unit 12 for holding. The reaction container 4 is washed and dried by the washing unit 12 and then used again for measurement.

Next, the configuration of the mechanism unit 31 of the analysis control unit 30 will be described. Although not shown, the mechanism unit 31 is a mechanism for rotating the sampler 6a, the reagent storage 2 and the reagent storage 3 of the analysis unit 19, and a rack 17a.
, Mechanism for rotating the reaction disk 5, sample dispensing arm 10, first reagent dispensing arms 8a, 8b, second reagent dispensing arms 9a, 9b, first stirring unit 11a, and second stirring unit A mechanism for rotating and vertically moving 11b and a mechanism for vertically moving the cleaning unit 12 are provided.

The mechanism unit 31 drives a sample dispensing pump 10a that sucks a sample into the sample dispensing probe 16 and discharges the sample from the sample dispensing probe 16 via a pressure transmission medium such as water. , A mechanism for driving each reagent pump for aspirating and discharging the first reagent from the first reagent dispensing probes 14a, 14b, and a reagent pump for aspirating and discharging the second reagent from the second reagent dispensing probes 15a, 15b. Each driving mechanism, the first stirring unit 11a
1A, 1B of each mechanism for separately driving the stirrer, 2A of the second stirring unit 11b
, Each mechanism for separately stirring the 2B stirrer, a mechanism for driving a cleaning pump for discharging and sucking cleaning liquid from the cleaning nozzle of the cleaning unit 12, and a drying pump for sucking from the drying nozzle of the cleaning unit 12 It has a mechanism.

The control unit 32 includes a sampler 6a, a rack 17a, a reagent store 2, a reagent store 3, a reaction disk 5, a sample dispensing arm 10, first reagent dispensing arms 8a and 8b, and a second reagent dispensing arm 9a. 9b, first stirring unit 11a, second stirring unit 11b, cleaning unit 12
And a control circuit for controlling each mechanism such as the sample dispensing pump 10a, the reagent pump, the 1A and 1B stirrers, the 2A and 2B stirrers, the washing pump, and the drying pump. And each mechanism of the mechanism part 31 is operated per analysis cycle.

Next, with reference to FIG. 1 thru | or FIG. 5, the detail of sample dispensing operation | movement which dispenses a test sample to the reaction container 4 is demonstrated.

FIG. 4 is a flowchart showing a sample dispensing process for dispensing a test sample into the reaction container 4 and a sample dispensing probe cleaning process performed after the sample dispensing process.

This sample dispensing step S50 is executed for each analysis cycle, and the sample dispensing A step S60 for dispensing the test sample stored in the sample container 17 at the test sample suction position into the reaction container 4A;
It comprises a sample dispensing B step S70 for dispensing the test sample into the reaction vessel 4B. In the sample dispensing A process S60 and the sample dispensing B process S70, the test sample is placed in the reaction container 4.
A movement of the sample dispensing probe 16 to be dispensed into A and 4B to the sample container 17, suction of the test sample through the sample dispensing probe 16, reaction container 4A of the sample dispensing probe 16 4B, and reaction vessels 4A, 4 through the sample dispensing probe 16
The operation consists of discharging to B.

In addition, during one analysis cycle, in addition to the sample dispensing step S50, the sample dispensing A step S6
0 or only the sample dispensing B step S70 can be performed.

The sample dispensing step S50 is based on a measurement instruction from the system control unit 70 to the control unit 32 of the analysis control unit 30, and the reaction disk 5, the sample dispensing arm 10, the sample dispensing controlled by the control unit 32. It is executed by the mechanism of the mechanism unit 31 that drives each analysis unit such as the pump 10a.

The sample dispensing step A60 in the sample dispensing step S50 includes steps S61 to S65.

First, the sample dispensing arm 10 is rotated in the direction of arrow R1 in FIG. 3 while the reaction disk 5 is rotating, and the sample dispensing probe 16 is moved from the sample dispensing probe cleaning position to the test sample suction position.
Is further lowered and stopped until the tip of the sample dispensing probe 16 detects the liquid level of the sample in the sample container 17 (step S61).

The sample dispensing pump 10a performs a suction operation for dispensing the test sample into the reaction container 4A that stops at the sample discharge position A after the sample dispensing arm 10 is lowered. By this suction operation, the test sample is sucked from the sample container 17 into the sample dispensing probe 16 (step S62).

Thus, since one sample dispensing probe 16 is moved and the test sample is sucked,
It is possible to easily move the sample container 17b, which is difficult to move with the two sample dispensing probes, to suck the test sample.

The sample dispensing arm 10 moves up after the sample dispensing pump 10a performs the sample suction operation, and then rotates in the direction of the arrow R2 in FIG. 3 to move the sample dispensing probe 16 to the sample discharge position A. After the reaction disk 5 stops rotating, the sample dispensing probe 1
6 is lowered and stopped until the tip of 6 reaches the discharge position in the reaction vessel 4A (step S63).
.

The sample dispensing pump 10a performs a discharge operation for dispensing the test sample to the reaction container 4A stopped at the sample discharge position A after the sample dispensing arm 10 is lowered. By this discharge operation, the test sample is discharged from the sample dispensing probe 16 into the reaction container 4A (
Step S64).

The sample dispensing arm 10 moves up after the test sample discharge operation of the sample dispensing pump 10a, and then rotates in the R1 direction to move the sample dispensing probe 16 to the sample dispensing probe cleaning position (step S65).

The sample dispensing B process S70 includes steps S71 to S75. The sample dispensing arm 10 rotates in the R1 direction to move the sample dispensing probe 16 from the sample dispensing probe cleaning position to the test sample suction position, and the tip of the sample dispensing probe 16 is inside the sample container 17. The liquid is lowered and stopped until the liquid level of the test sample is detected (step S71).

The sample dispensing pump 10a performs a suction operation for dispensing the test sample to the reaction container 4B stopped at the sample discharge position B after the sample dispensing arm 10 is lowered. By this suction operation, the test sample is sucked from the sample container 17 into the sample dispensing probe 16 (
Step S72).

The sample dispensing arm 10 moves up after the sample dispensing operation of the sample dispensing pump 10a, and then rotates in the R2 direction to move the sample dispensing probe 16 to the sample discharge position B.
Further, the sample dispensing probe 16 descends and stops until it reaches the discharge position in the reaction container 4B (step S73).

The sample dispensing pump 10a is operated after the sample dispensing arm 10 is lowered.
A discharge operation for dispensing the test sample is performed. By this discharge operation, the test sample is discharged from the sample dispensing probe 16 into the reaction container 4B (step S74).

The sample dispensing arm 10 moves up after the test sample discharge operation of the sample dispensing pump 10a, and then rotates in the R2 direction to move the sample dispensing probe 16 to the sample dispensing probe cleaning position (step S75).

Next, in the next analysis cycle when sample dispensing of the last measurement item of the same test sample is completed,
A sample dispensing probe washing step S80 for washing the sample dispensing probe 16 is performed.

The sample dispensing probe cleaning step S80 includes steps S81 to S83.
The sample dispensing arm 10 descends and stops until the tip of the sample dispensing probe 16 reaches the cleaning liquid discharge port provided at the sample dispensing probe cleaning position (step S81).

The sample dispensing pump 10a is supplied via a switching valve provided in the flow path of the pressure transmission medium between the sample dispensing pump 10a and the sample dispensing probe 16 after the sample dispensing arm 10 is lowered. The cleaning liquid from the flow path is discharged from the sample dispensing probe 16 to clean the inner wall. Further, the outer wall of the sample dispensing probe 16 is cleaned by discharging the cleaning liquid from the cleaning liquid discharge port at the sample dispensing probe cleaning position (step S82).

After the sample dispensing probe 16 is cleaned, the sample dispensing arm 10 rises and waits for the next dispensing of the test sample (step S83).

FIG. 5 shows a sample dispensing step S50 and a sample dispensing probe cleaning step 80 shown in FIG.
It is a timing chart which showed the timing of the operation | movement of each of these steps.

In the timing chart 80, the sample dispensing A process S60 executed in the arrow T direction is shown.
Steps S61 to S65, steps S71 to S75 of the sample dispensing B step S70,
In addition, the operation start timing, operation time, and operation end timing of the reaction disk 5, the sample dispensing arm 10, and the sample dispensing pump 10a corresponding to steps S81 to S83 of the sample dispensing probe cleaning process 80 are shown.

First, details of the operation timing of each step corresponding to the sample dispensing step S50 of the timing chart 80 will be described.

Steps S62, S64 in the sample dispensing step S50 of the timing chart 80
The “suction” and “discharge” of the convex portions in S72 and S74 correspond to the suction and discharge operations of the sample dispensing pump 10a, and the width of the convex portion corresponds to the operation time. When the maximum sample amount is set as the analysis condition for each measurement item, the time required for sucking and discharging the maximum amount is assigned to the operation time. Therefore, since the operation start timing of “suction” and “discharge” does not change for each analysis cycle, when the set sample amount is smaller than the maximum amount, the sample dispensing arm 10 and the sample dispensing pump 10 a The same state is maintained during the surplus time from the actual operation end timing indicated by the broken line to the assigned operation end timing.

Steps S61, S63, S65, S71, S73, and S75 are the same as the sample dispensing arm 1
This corresponds to the movement of the sample dispensing probe 16 by the zero rotation, ascending and descending operations.

The “movement” of the convex portions in steps S61 and S71 is the movement from the sample dispensing probe cleaning position to the test sample suction position by the rotation of the sample dispensing arm 10, and the sample dispensing arm 1
This corresponds to the movement from the test sample suction position to the liquid level detection position of the test sample by descending 0.

“Movement” in step S 63 is a movement from the test sample liquid level detection position to the test sample suction position by raising the sample dispensing arm 10, and a sample discharge from the test sample suction position by turning the sample dispensing arm 10. This corresponds to the movement to the position A and the movement from the sample discharge position A to the discharge position in the reaction container 4A due to the lowering of the sample dispensing arm 10.

In step S63, in order to prevent the sample dispensing probe 16 from colliding with the rotating reaction disk 5, the discharge from the sample discharge position A into the reaction container 4A is synchronized with the stop timing of the reaction disk 5. The sample dispensing probe 16 is moved to the position.
For this reason, the timing of step S62 is assigned in accordance with the timing of step S63, so that there is some margin time in steps S61 and S62. Therefore, the sample dispensing A process S60 requires a longer time than the sample dispensing B process S70.

The “movement” in step S65 is a movement from the discharge position of the reaction container 4A to the sample discharge position A due to the ascent of the sample dispensing arm 10 and a sample dispensing probe from the sample discharge position A due to the rotation of the sample dispensing arm 10. It corresponds to the movement to the cleaning position.

The “movement” in step S71 is a movement from the sample dispensing probe cleaning position to the test sample suction position by the rotation of the sample dispensing arm 10 and a test from the test sample suction position by the lowering of the sample dispensing arm 10. It corresponds to the movement of the sample to the liquid level detection position.

“Movement” in step S73 is the movement of the test sample from the liquid level detection position to the test sample suction position by raising the sample dispensing arm 10, and the sample from the test sample suction position by rotating the sample dispensing arm 10 This corresponds to the movement to the discharge position B and the movement from the sample discharge position B to the discharge position in the reaction container 4B by the lowering of the sample dispensing arm 10.

The “movement” in step S75 is a movement from the discharge position of the reaction container 4B to the sample discharge position B due to the ascent of the sample dispensing arm 10 and a sample dispensing probe from the sample discharge position B due to the rotation of the sample dispensing arm 10. It corresponds to the movement to the cleaning position.

Each “movement” in each step is accelerated and moved in the initial stage, then moved at a constant high speed, further decelerated and temporarily stopped, and then proceeds to the next step. Step S
When steps S71 and S61 are executed in succession to 65 and S75, the sample dispensing probe 16 is accelerated at the initial stage of steps S65 and S75, and decelerated in the vicinity of the test sample suction position in steps S71 and S61. Move at a constant high speed.

Next, the “movement” of the convex portion of step S81 in the sample dispensing probe cleaning process 80 is performed from the sample dispensing probe cleaning position of the sample dispensing probe 16 by the lowering of the sample dispensing arm 10 to the sample dispensing probe cleaning position. It corresponds to the movement to the cleaning liquid discharge port.

The “cleaning” of the convex portion in step S82 corresponds to the cleaning of the inner and outer walls of the sample dispensing probe 16 in contact with the test sample by the sample dispensing pump 10a or the like.

The “movement” of the convex portion in step S83 corresponds to the movement of the sample dispensing probe 16 from the cleaning liquid discharge port at the sample dispensing probe washing position to the sample dispensing probe washing position due to the rising of the sample dispensing arm 10. .

The width of the “moving” convex portion of each step corresponds to the moving time, and the width of the “cleaning” convex portion corresponds to the cleaning time.

Next, the relationship between the operation timing of each step of the sample dispensing process S50 of the timing chart 80 and the operation timing of the reaction disk 5 will be described.

The reaction disk 5 rotates and stops at every analysis cycle from the start to the end of measurement.
Then, during the rotation for each analysis cycle, steps S61 and 62 and the middle of “movement” in step S63 are executed. Next, during the stop of each analysis cycle of the reaction disk 5, at least “movement” from the sample discharge position A to the discharge position in the reaction container 4A in step S63, and steps S64, S65, S71, S72, S73, S74, S75 is executed.

Then, steps S81, S82, and S83 are executed during the rotation of the reaction disk 5 in the next analysis cycle after the dispensing operation of the same test sample is completed.

In addition, if the test sample includes immunity items of extremely low concentration that are easily affected by other test samples, for example, by setting the measurement item as an easily affected item in advance,
Within the analysis cycle after dispensing the test sample before the test sample containing sensitive items,
The sample dispensing probe 16 is cleaned over a longer time than the normal cleaning time in step S82.

In this way, the reaction vessel 4 is rotated during the rotation of the reaction disk 5 that rotates and stops at each analysis cycle.
A test sample to be dispensed into A is aspirated, and the test sample is placed in the reaction vessel 4 while the reaction disk 5 is stopped.
The sample dispensing probe 16 is further moved between the sample suction position and the sample discharge position B at high speed to suck the test sample from the sample container and discharge it to the reaction container 4B, thereby dispensing the sample. The test sample can be dispensed into the reaction vessels 4A and 4B for each analysis cycle using only one sample dispensing system such as the probe 16, the sample dispensing arm 10, and the sample dispensing pump 10a.

Next, the operation of the automatic analyzer 100 will be described with reference to FIGS. FIG. 6 is a flowchart showing an example of the operation of the automatic analyzer 100.

A storage unit 42 of the analysis data processing unit 40 is obtained by a calibration operation from the operation unit 60.
Stores a calibration table for each measurement item in advance. Further, the operation unit 60
The measurement item selected and input for each test sample is stored in the internal storage circuit of the system control unit 70 by the measurement item selection input from.

FIG. 7 is a diagram illustrating an example of a measurement item setting screen that is selectively input and displayed on the display unit 52. The measurement item setting screen 53 includes an “ID” column for displaying the ID of the subject input on the subject information input screen, an “item” column for displaying the item name in an abbreviated name, and an “ID” column. The measurement item setting area 53a for setting the measurement item of the item name displayed in the “item” column for each subject ID displayed in FIG.

In the “ID” column, for example, IDs of subjects “1” to “3” input in advance are displayed. In the “item” column, for example, “GOT” and “Ca” to be measured in the reaction vessel 4A in advance by setting the analysis condition of the measurement line A, and reaction by setting the analysis condition of the measurement line B. Item names such as “GPT” and “TP” to be measured in the container 4B are displayed.

In the measurement item setting area 53a, “◯” is displayed when the item name in the “item” column corresponding to the ID of each subject in the “ID” column is set, and “0” is displayed when the item name is not set.・ ”Is displayed. Then, for example, “G” displayed in the “ID” column from the operation unit 60 is “G”.
When “OT”, “GPT”, “Ca”, and “TP” are set, the measurement item setting area 53
“a” of 1 to 4 tests is displayed in a.

When “TP” is set for “2” displayed in the “ID” column, “○” of 5 tests is displayed in the measurement item setting area 53a and displayed in the “ID” column. When “Ca” is set for “3”, “◯” for 6 tests is displayed in the measurement item setting area 53a.

The measurement item setting information set and input from the measurement item setting screen 53 is stored in the internal storage circuit of the system control unit 70.

The measurement of the test sample is performed based on the measurement item information selected and input from the measurement item setting screen 53. The measurement is performed in order from the measurement item (measurement item) set to the left of the ID of each subject in order from the test sample corresponding to “1” in the top row of “ID”.

First, by the measurement start operation from the operation unit 60, the automatic analyzer 100 starts operation (step S1 in FIG. 6).

The system control unit 70 instructs the analysis control unit 30, the analysis data processing unit 40, and the output unit 50 to instruct measurement of each measurement sample stored in the internal storage circuit. The control unit 32 of the analysis control unit 30 starts the measurement operation after temporarily moving each analysis unit of the analysis unit 19 to the home position (step S2 in FIG. 6).

The analysis unit 19 measures “GOT” of the subject ID “1” selected and input on the measurement item setting screen 53 during one analysis cycle shown in FIG. 8 after each analysis unit starts the measurement operation. A sample dispensing A step S60 for dispensing the test sample and a sample dispensing B step S70 for measuring “GPT” are executed (step S3 in FIG. 6).

Sample Dispensing A Process S60 for dispensing the test sample of “GOT” with the subject ID “1”
Then, the sample dispensing arm 10 rotates in the R1 direction while the reaction disk 5 rotates, and moves the sample dispensing probe 16 from the sample dispensing probe cleaning position to the test sample suction position. Then, it stops at the test sample suction position, for example, descends to the liquid level detection position of the test sample of the test subject ID “1” housed in the sample container 17 at the position “1” of the rack 17a in FIG. .

The sample dispensing pump 10 a performs an operation of sucking a test sample “GOT” (one test in FIG. 7) into the sample dispensing probe 16.

The test sample is sucked and discharged from the sample dispensing probe 16 through a pressure transmission medium such as water sealed in a flow path between the sample dispensing pump 10a and the sample dispensing probe 16. Between the test sample of a set sample amount of one measurement item sucked into the sample dispensing probe 16 and a pressure transmission medium, a dummy layer is provided by the test sample that is not used for measurement. This dummy layer prevents dilution of the sample to be measured by the pressure transmission medium.

Then, when the test sample of the first measurement item for each test sample is sucked, the dummy test sample is first sucked, and then the test sample of the set sample amount of the measurement item is sucked, and the test sample is discharged. Sometimes the dummy test sample is held in the sample dispensing probe 16. From the second and subsequent measurement items, the test sample of the set sample amount of the measurement item is aspirated and the sample dispensing probe 16 is drawn.
The dummy held inside is reused in the sample dispensing probe 16 until dispensing of all measurement items of the test sample is completed.

Thus, since the test sample is sucked using one sample dispensing probe 16, the amount of dummy test sample used can be reduced. Further, since the test sample for one measurement item is sucked into the sample dispensing probe 16, it is possible to prevent the test sample of the measurement item from being diluted in the sample dispensing probe 16.

The sample dispensing arm 10 rises and rotates in the R2 direction after the test sample suction operation of the sample dispensing pump 10a. While the reaction disk 5 is stopped, the sample dispensing probe 16 is lowered and moved to the discharge position of the reaction container 4A stopped at the sample discharge position A.

The sample dispensing pump 10 a discharges the “GOT” test sample to the reaction container 4 </ b> A via the sample dispensing probe 16 after the movement operation of the sample dispensing arm 10.

The sample dispensing arm 10 moves up and rotates in the R1 direction after the sample dispensing operation of the sample dispensing pump 10a to move the sample dispensing probe 16 to the sample dispensing probe cleaning position.

Sample Dispensing B Step S70 for dispensing the test sample of “GPT” with the subject ID “1”
Is executed continuously from the sample dispensing A step S60.

The sample dispensing arm 10 rotates in the R1 direction to move the sample dispensing probe 16 from the sample dispensing probe cleaning position to the test sample suction position. Then, the position of the rack 17a “
1 ”is lowered to the liquid level detection position of the test sample and stopped.

The sample dispensing pump 10 a performs an operation of sucking a test sample “GPT” (2 test in FIG. 7) into the sample dispensing probe 16.

The sample dispensing arm 10 rises, rotates in the R2 direction, and descends after the sample dispensing pump 10a performs the sample suction operation to stop the sample dispensing probe 16 at the sample discharge position B. Move to the 4B discharge position.

The sample dispensing pump 10 a discharges the test sample “GPT” to the reaction container 4 </ b> B via the sample dispensing probe 16 after the movement operation of the sample dispensing arm 10.

The sample dispensing arm 10 moves up and rotates in the R1 direction after the sample dispensing operation of the sample dispensing pump 10a to move the sample dispensing probe 16 to the sample dispensing probe cleaning position.

Next, the analysis unit 19 dispenses the test samples “Ca” and “TP” of the subject ID “1” in the same manner as the operation in the first analysis cycle in the second analysis cycle of FIG. The sample dispensing step S50 is executed (step S4 in FIG. 6).

Next, the analysis unit 19 performs the sample dispensing probe 16 during the three analysis cycles shown in FIG.
A sample dispensing probe cleaning step S80 is performed to wash off the test sample with the test subject ID “1” attached to (step S5 in FIG. 6).

The sample dispensing arm 10 descends and stops until the tip of the sample dispensing probe 16 reaches the cleaning liquid discharge port provided at the sample dispensing probe cleaning position.

The sample dispensing pump 10a cleans the inner wall of the sample dispensing probe 16 after the sample dispensing arm 10 is lowered. Further, the outer wall of the sample dispensing probe 16 is washed with the washing liquid from the washing liquid discharge port at the sample dispensing probe washing position.

As described above, since the inner and outer walls of the sample dispensing probe 16 in contact with the test sample are washed after the test sample dispensing operation with the subject ID “1” is completed, the subject is measured via the sample dispensing probe 16. The amount of the test sample brought from the ID “1” to the subject ID “2” can be reduced.

The sample dispensing arm 10 rises after the sample dispensing probe 16 is cleaned, and the subject ID
Wait for the dispensing of the test sample of “2”.

Next, the analysis unit 19 executes a sample dispensing B step S70 for dispensing a test sample for measuring “TP” of the subject ID “2” in the four analysis cycles of FIG. 8 (FIG. 8). 6 step S6).

Then, the subject I attached to the sample dispensing probe 16 during the five analysis cycles of FIG.
A sample dispensing probe washing step S80 for washing off the test sample of D “2” is executed (step S7 in FIG. 6).

Next, the analysis unit 19 executes the sample dispensing A step S60 for dispensing the test sample for measuring “Ca” of the subject ID “3” during the six analysis cycles of FIG. 8 (FIG. 8). 6 step S8).

Then, the subject I attached to the sample dispensing probe 16 during the seven analysis cycles of FIG.
A sample dispensing probe washing step S80 for washing off the test sample of D “3” is executed (step S9 in FIG. 6).

After dispensing the test sample, the reaction container 4A into which the test samples with the subject IDs “1” to “3” are dispensed,
When 4B stops at the first reagent discharge positions A and B, the first reagent corresponding to the measurement item of each reaction container 4A and 4B is dispensed via the first reagent dispensing probes 14a and 14b (FIG. 6). Step S10).

After the first reagent is dispensed, when the reaction vessels 4A and 4B containing the mixed solution of the test sample with the test IDs “1” to “3” and the first reagent stop at the first stirring positions A and B, The mixed liquid in each reaction container 4A, 4B is stirred by the 1A, 1B stirrer of the 1 stirring unit 11a (step S11 in FIG. 6).

After the first stirring, when the reaction containers 4A and 4B containing the mixed solution of the test sample with the test IDs “1” and “3” and the first reagent stop at the second A and second B reagent discharge positions, The second reagent corresponding to the measurement item of the two reagent system “GOT”, “GPT”, “Ca” is dispensed to each reaction container 4A, 4B via the two reagent dispensing probes 15a, 15b (FIG. 6). Step S12).

After dispensing the second reagent, the reaction containers 4A and 4B containing the test samples of the test IDs “1” and “3”, the first reagent, and the mixed solution of the second reagent are moved to the second stirring positions A and B. When stopped, the liquid mixture in the reaction vessels 4A and 4B is stirred by the second A and 2B stirrers of the second stirring unit 11b (step S13 in FIG. 6).

After the second agitation, photometry is performed when the mixture of the test sample, the first reagent, and the second reagent, or the reaction containers 4A and 4B containing the test sample and the first reagent are passed through the photometry position. The unit 13 irradiates the reaction vessels 4A and 4B with light, and measures the absorbance at the set wavelength from the transmitted light. Then, an analysis signal of each measurement item of the test sample with the subject ID “1” to “3” is generated and output to the analysis data processing unit 40 (step S14 in FIG. 6).

After the measurement, the reaction containers 4A, 4 containing a mixture of the test samples having the test IDs “1” to “3”
When B stops at the cleaning and drying position, the cleaning unit 12 aspirates the mixed liquid after the measurement in the reaction containers 4A and 4B and cleans and drys the reaction container 4 (step S15 in FIG. 6). . And reaction container 4A, 4B which finished washing | cleaning and drying is used for a measurement again.

The calculation unit 41 of the analysis data processing unit 40 generates analysis data of each measurement item of each test sample from the analysis signal output from the photometry unit 13 and stores the analysis data in the storage unit 42, and the output unit 5
It outputs to 0 (step S16 in FIG. 6).

When the cleaning and drying of all the reaction containers 4 are completed and the analysis data of all the measurement items of the subject IDs “1” to “3” are output, the automatic analyzer 100 ends the measurement operation. (Step S17 in FIG. 6).

  Next, another embodiment of the timing chart 80 will be described with reference to FIGS.

FIG. 9 is a timing chart showing another embodiment of the timing chart 80 shown in FIG. The timing chart 81 differs from the timing chart 80 in that the operation start and operation end timings of “suction” and “discharge” while the reaction disk 5 is stopped, in which the time of the sample dispensing operation is restricted, are made variable. .

The timing chart 81 includes steps S61, S62, S63, S64a, and S65a for dispensing the test sample to the reaction vessel 4A, and steps for dispensing the test sample to the reaction vessel 4B. “Suction” in S71, S72a, S73, S74a, S75,
The timing of “discharge” and “movement” is shown. The timing chart 81
Steps S64a, S72a, and S74a are the same as Step S64 of the timing chart 80.
, S72 and S74, the operation start and end timings of each step are made variable.

By the way, there is a possibility that the maximum sample amount may be set as the analysis condition of the measurement item, and if the measurement item is not selected, the sample to be tested is the maximum in the sample dispensing step S50. It is not dispensed in large quantities. For example, if the maximum sample amount is 15 μL, the average of the set sample amounts of the analysis conditions for each measurement item is usually 3 μL to 4 μL.

Accordingly, when dispensing measurement items in which a small sample amount of, for example, 1.5 to 4.0 μL is likely to be deteriorated, excessive “suction” and “discharge” described in FIG. 5 is performed. The time is allocated to a factor that causes an error in dispensing accuracy, which will be described later.

Before executing the sample dispensing step S50, the system control unit 70 reads the set sample amount of the measurement item to be dispensed into the reaction vessels 4A and 4B in the sample dispensing step S50 from the internal storage circuit, and in step S64a Discharge time TAd required for discharge into the reaction vessel 4A,
A suction time TBa required for sucking the sample to be dispensed into the reaction container 4B in step S72 and a discharge time TBd required for discharge to the reaction container 4B in step S74 are calculated.

Then, assuming that the time required for suctioning the maximum sample amount is the maximum sample suction time Tma and the time required for discharging the maximum sample amount is the maximum sample discharge time Tmd, step S64a,
In S72a and S74a, (Tmd-TAd), (Tma? TBa), and (Tmd-T), respectively.
The surplus time represented by Bd) is born. This surplus time is allocated to the dispensing accuracy error factor described below.

The surplus time in steps S64a, S72a, and S74a is set to Tw1, Tw3, T on the front
It is divided into w5 and rear Tw2, Tw4, Tw6.

Then, the front side Tw1, Tw of the “ejection” surplus time in steps S64a, S74a
5 for example, adjusting the descending speed when the sample dispensing arm 10 is lowered, the sample dispensing arm 1
It is assigned to adjust the waiting time until the sample dispensing pump 10a starts the discharge operation of the test sample after the descent operation of 0.

Adjustment of the waiting time until the sample dispensing arm 16 starts the ascending operation after the test sample dispensing operation of the sample dispensing pump 10a, for example, the rear side Tw2, Tw6 of the “ejection” surplus time in steps S64a, S74a, The sample dispensing arm 10 is allocated for adjusting the ascent speed when the sample dispensing arm 10 is raised.

The front side Tw3 of the “suction” surplus time in step S72a is adjusted, for example, by adjusting the descending speed when the sample dispensing arm 10 is lowered, and after the sample dispensing arm 10 is lowered, the sample dispensing pump 10a performs the suction operation of the test sample. Allocate to adjust the waiting time until starting.

For example, adjustment of the waiting time until the sample dispensing arm 16 starts the ascending operation after the sample aspirating operation of the sample dispensing pump 10a is performed on the rear side Tw4 of the “suction” surplus time in step S72a, the sample dispensing arm Allocate to adjust the ascending speed at 10 ascending.

As described above, the operation timing of “suction” and “discharge” in the timing chart during the stop of the reaction disk 5 is made variable based on the set sample amount of the analysis condition, and the surplus time generated by the sample dispensing of the minute sample is set. By assigning to operations that cause errors in dispensing accuracy, the sample dispensing accuracy of a small amount of sample can be improved.

FIG. 10 is a timing chart showing another embodiment of the timing chart 80 shown in FIG. The timing chart 82 is different from the timing chart 80 in that the “movement” operation start timing when the reaction disk 5 is stopped and the sample dispensing operation is restricted, and “
The operation start and end timings of “suction” and “discharge” are made variable.

The timing chart 82 includes steps S61, S62, S63, S64b, and S65b for dispensing the test sample to the reaction vessel 4A, and steps for dispensing the test sample to the reaction vessel 4B. The timings of “suction”, “discharge”, and “movement” in S71b, S72b, S73b, S74b, and S75b are shown.

Then, the operation end timing in step S64b of the timing chart 82, the operation start and end timings in steps S72b and 74b, and steps S65b, S73b and S
The operation start timing of 75b is variable, and steps S64b, S72b, S7
The operations of steps S64b and S65b and steps SS71b to S75b are continuously performed except for the surplus time generated in 4b.

The timing of starting the discharge of the test sample into the reaction vessel 4A in step S64b, which is executed while the reaction disk 5 is stopped, is a predetermined time t1 after the timing of starting the rotation of the reaction disk 5, and from the timing of starting the discharge in step S64b. The time until the next rotation start timing of the reaction disk 5 is set to a certain maximum time Tmax.

In addition, in (Step S65b + Step S71b) and Step S73b, the movement time between the discharge position of the sample dispensing probe 16 in the reaction container 4A and the sample container 17 between the test sample and the reaction container 4B of the sample dispensing probe 16 in Step S73b. Movement time between the discharge position in the sample container and the sample container 17 and the sample dispensing probe 1 in step S75b
The moving time between the discharge position in the reaction container 4B and the sample dispensing probe cleaning position is set to constant TAt, TBt, and Th1, respectively. In addition, “Steps S64b, S72b, S74b”
The operation time of “discharge” and “suction” is calculated in advance by the system control unit 70 every time the sample analysis step 50 is executed, and is set to variable TAd, TBa, TBd for each measurement item.

The maximum time Tmax is Tmax ≧ (TAd + TAt + TBa + TBt + TBd
+ TBt1), and the maximum time Tmax is (TAd + TAt + TBa + TBt).
Coincides with the timing chart 80 when equal to + TBd + TBt1).

FIG. 11 applies the timing chart 82 in which the test sample is dispensed to the reaction vessels 4A and 4B in FIG. 10 when only the test sample is dispensed to the reaction vessel 4B in the analysis cycle. It is a timing chart.

This timing chart 82a shows the timing of “movement”, “suction”, and “discharge” in steps S71c, S72c, S73c, S74c, and S75c for dispensing the test sample into the reaction vessel 4B. Steps S61, S62, S63, S64b,
It almost coincides with the timing of S65b.

FIG. 12 is a timing chart showing the timing of “cleaning” performed after the dispensing operation of the test sample to the reaction vessels 4A and 4B in FIG.

In this timing chart 82b, variable “movement” of step S81b, “washing” of step S82b, and “movement” of step S83b, which are continuously performed after dispensing the test sample into the reaction vessel 4A or 4B. Timing of the sample dispensing probe cleaning step S
This corresponds to 80 steps S81, S82, and S83.

The operating times of steps S81b, S82b, and S83b are set to constant Th2, Tw, and Th3, respectively.
Then, Tmax ≧ (TAd + TAt + TBa + TBt + TBd + Th1 + Th2 + T
At the time of w + Th3), since “cleaning” can be performed while the reaction disk 5 is stopped, the timing chart 82b is executed. Tmax <(TAd + TAt + TBa + TBt + T
Bd + Th1 + Th2 + Tw + Th3), Steps S81, S82, and S83 of the timing chart 80 are executed.

In this way, the operation start and end timings of “suction” and “discharge” in the timing chart, the operation start timing of “movement”, and the operation start timing of “cleaning” are made variable,
“Move” while the reaction disk 5 is stopped, except for the extra time caused by dispensing a small amount of sample.
, “Discharge”, “Suction”, and “Washing” are successively executed to dispense the test sample into the reaction vessels 4A and 4B and to clean the sample dispensing probe 16 within the analysis cycle. Therefore, when measuring N test samples, the measurement time can be maximized and the measurement time of the (N-1) analysis cycle can be shortened.

According to the embodiment of the present invention described above, the sample dispensing probe 1 in the semi-random access system having the A and B measurement lines provided so as to be able to measure two measurement items.
6. Using one sample dispensing system such as the sample dispensing arm 10 and the sample dispensing pump 10a, for dispensing into the reaction vessel 4A during the rotation of the reaction disk 5 that rotates and stops at each analysis cycle. The test sample can be sucked, the test sample can be discharged into the reaction container 4A while the reaction disk 5 is stopped during the analysis cycle, and the test sample can be sucked and discharged into the reaction container 4B. Thereby, since the influence between the test samples via the sample dispensing probe 16 can be reduced, it can measure with a sufficient precision. Moreover, by using one sample dispensing system, the configuration of the apparatus can be simplified and the cost can be reduced.

Further, by aspirating the test sample using one sample dispensing probe 16, 2
The sample to be tested can be sucked easily by moving the sample container 17b, which is difficult to move with one sample dispensing probe. Moreover, since the usage-amount of a dummy can be reduced, it can measure with a trace amount test sample.

Further, by aspirating the test sample for one measurement item into the sample dispensing probe 16, dilution of the test sample of the measurement item in the sample dispensing probe 16 is prevented, and the test sample is accurately separated. Since it can be poured, it can be measured accurately.

Further, the operation timings of “suction” and “discharge” while the reaction disk 5 is stopped are made variable based on the set sample amount of the analysis condition, and the surplus time generated by dispensing a small amount of sample is determined as an error in sample dispensing accuracy. By allocating to the action of the factor, the sample dispensing accuracy of a very small amount of sample can be further improved.

In addition, the start and end timings of “suction” and “discharge” while the reaction disk 5 is stopped, the operation start timing of “movement”, and the operation start timing of “cleaning” are made variable so that a minute sample can be separated. The reaction vessels 4A and 4B are operated within the analysis cycle by continuously operating “movement”, “discharge”, “suction”, and “cleaning” while the reaction disk 5 is stopped, except for the surplus time caused by the injection. Since the test sample can be dispensed and the sample dispensing probe 16 can be washed, the measurement time can be shortened.

In addition, this invention is not limited to the said Example, For example, in addition to A and B measurement line, C measurement line is provided, One sample dispensing system, and reaction container 4C corresponding to C measurement line
In addition, it may be carried out by a semi-random system provided with a reagent dispensing system such as a reagent dispensing probe, a reagent dispensing arm, a reagent dispensing pump, and a stirring unit.

In this case, the operation start and end timings of “suction” and “discharge” while the reaction disk is stopped and the operation start timing of “movement” are made variable so that the reaction container is rotated during the rotation of the reaction disk 5 for each analysis cycle. “Aspiration” for dispensing into 4A, “discharge” into the reaction vessel 4A while the reaction disk 5 is stopped, “movement”, “to move” to dispense the test sample into the reaction vessels 4B and 4C
By continuously operating “discharge” and “suction”, the same effect as the one sample dispensing system can be obtained, and the test sample can be further processed at high speed.

Also, for example, four A1, B1, A2, and B2 measurement lines, reaction vessels 4A1, 4A2 and A sample dispensing probes corresponding to the A1, A2 measurement lines, and reaction vessels 4B1, 4B2 corresponding to the B1, B2 measurement lines A and B sample dispensing probes, a sample dispensing arm that moves the A and B sample dispensing probes, and A and B sample dispensing that perform suction and discharge of the test sample through the A and B sample dispensing probes, respectively. A pump, four first and second reagent dispensing systems (reagent dispensing probe, reagent dispensing arm, and reagent dispensing pump) corresponding to the A1, B1, A2, and B2 measurement lines, and four stirring bars A first stirring unit and a second stirring unit.

While the reaction disk is rotating, the test sample to be dispensed into the reaction vessels 4A1 and 4B1 is sucked using the A and B sample dispensing probes and the A and B sample dispensing pumps, and the reaction disk is stopped. In addition, the reaction container 4 was prepared using the A sample dispensing probe and the A sample dispensing pump.
The test sample is discharged into A1, and the test sample is discharged into the reaction vessel 4B1 using the B sample dispensing probe and the B sample dispensing pump.

Furthermore, the sample dispensing arm is moved to cause the test sample to be dispensed into the reaction vessel 4A2 using the A sample dispensing probe and the A sample dispensing pump, and at the same time, using the B sample dispensing probe and the B sample dispensing pump. Then, the test sample is dispensed into the reaction vessel 4B2.

Thereby, since the influence between test samples via a sample dispensing probe can be reduced rather than the case where the same number of sample dispensing probes as a measurement line is used, it can measure accurately. Further, the configuration of the apparatus can be simplified and the cost can be reduced. Moreover, since the usage-amount of a dummy can be reduced, it can measure with a trace amount test sample. In addition, by aspirating the test sample for one measurement item into each sample dispensing probe, dilution of the test sample of the measurement item in the sample dispensing probe is prevented, and the test sample is accurately dispensed. Can be measured with high accuracy.

4, 4A, 4B Reaction vessel 5 Reaction disk 10 Sample dispensing arm 16 Sample dispensing probe 17 Sample container 17a Rack 19 Analytical unit 20 Sample unit 21 Reagent unit 22 Reaction unit 30 Analysis control unit 31 Mechanism unit 32 Control unit 40 Analysis data Processing unit 50 Output unit 60 Operation unit 70 System control unit 100 Automatic analyzer

Claims (2)

A dispensing means for sucking the test sample from the sample container containing the test sample and discharging it into the reaction container;
When the test sample is discharged into the reaction container by the first sample amount, the first suction time required to suck the test sample from the sample container by the dispensing means by the dispensing means. The first discharge time required for the second sample is less than the first sample amount from the sample container.
The first sample amount is based on the second suction time required for sucking the sample amount of the second sample and the second discharge time required for discharging the test sample by the second sample amount into the reaction container. A calculating means for calculating a surplus time which is a difference between a time required for dispensing a minute and a time required for dispensing the second sample amount;
Control means for varying the control of the dispensing means based on the surplus time;
An automatic analyzer characterized by comprising. The control means is a lowering action of the probe of the dispensing means to the sample container in the suctioning action of the test sample by the dispensing means, an aspiration action of the sample after stopping the lowering action of the probe, The ascending operation of the probe from the sample container after completion of the aspirating operation, the descending operation of the probe of the dispensing means to the sample container among the discharging operations of the sample to be tested by the dispensing means, the probe 2. The control of at least one of the discharge operation of the sample after stopping the lowering operation and the rising operation of the probe from the sample container after the discharge operation ends is varied. Automatic analyzer.

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