JP6071384B2 - Automatic analyzer - Google Patents

Description

  Embodiments described herein relate generally to an automatic analyzer that analyzes a component contained in a liquid such as a test sample collected from a subject.

  The automatic analyzer is intended for biochemical test items, immunological test items, etc., and optically detects changes in color and turbidity caused by the reaction of the mixture of the test sample collected from the sample and the reagent used for analysis of each test item. Measure automatically. By this measurement, analysis data represented by the concentration of various test item components in the test sample, the activity of the enzyme, and the like are generated.

  The automatic analyzer is provided with a plurality of reaction vessels that move and stop at regular cycle times. The sample dispensing probe sucks the test sample from the sample container and discharges it to the reaction container that stops at the sample discharge position. In addition, the reagent dispensing probe sucks a reagent for analysis of test items set according to the test from the reagent container and discharges it to the reaction container stopped at the reagent discharge position. Further, the stirring bar stirs the test sample that stops at the stirring position and the mixed solution in the reaction container from which the reagent has been discharged. The measuring unit measures the mixed liquid in the stirred reaction vessel. The cleaning nozzle cleans the reaction container that stops at the cleaning position after a predetermined time has elapsed since the sample or reagent was discharged.

  By the way, the reaction time of the test sample and the reagent differs for each inspection item. For inspection items that require a long reaction time, the reaction is interrupted halfway through the cleaning of the reaction container after a predetermined time has elapsed, and therefore it is conceivable to extend the reaction time by increasing the cycle time. However, since most test items do not require a long reaction time, there is a problem that throughput is lowered by increasing the cycle time. In response to this problem, multiple cleaning nozzles that wash the reaction vessel are moved independently to stop the washing of the reaction vessel that requires a long reaction time, thereby extending the reaction time, thereby reducing the decrease in throughput. There is an automatic analyzer that can.

  The number of reagents differs for each test item, and most test items consist of a first reagent of one reagent system or a first reagent and a second reagent of a two reagent system, and two first and second reagent dispensing probes. Dispensing is performed. For example, the number of first and second reagent dispensing probes that are larger than the number of first and second reagent dispensing probes, for example, can be automatically dispensed with the first and second reagent dispensing probes. There is an analyzer.

JP-A-6-160398 JP 2005-164508 A

  However, in the automatic analyzer capable of extending the reaction time, there is a problem that the maximum time of the first reaction by the sample and the first reagent is fixed and the time of the first reaction cannot be extended. In addition, there is a problem that a larger number of reagents than the number of reagent dispensing probes cannot be dispensed. In addition, there is a problem that the reaction time cannot be extended in an automatic analyzer capable of dispensing a larger number of reagents than the number of reagent dispensing probes.

  The embodiment has been made to solve the above problems, and an object thereof is to provide an automatic analyzer that can extend the reaction time of a larger number of reagents than the number of reagent dispensing probes.

To achieve the above object, an automatic analyzer of the embodiment, in the automatic analyzer for measuring the mixture of sample and reagent at a predetermined measurement position, the cleaning position, the sample discharge position and moved for each cycle time , a reagent discharge position, and a stirring position stops at including the stop position, the reaction vessel moves to and stops at the stop position has been stopped before the cycle time of the predetermined number of times or after a predetermined number of cycle time, the washing of the reaction vessel was stopped at the position, a cleaning nozzle for cleaning a reaction vessel in which the mixture has finished the measurement is accommodated, of the reaction vessel was stopped at the sample discharge position, cleaning line according to the cleaning nozzle a sample dispensing probe for ejecting the sample into a reaction vessel reaction vessel or the reagent of the air is contained in our out of the reaction vessel was stopped at the reagent discharging position, said cleaning A reagent dispensing probe washing with nozzle performed by discharging the reagent into a reaction vessel that is accommodated sample discharged by the air in the reaction vessel or the sample dispensing probe, the reaction vessel was stopped by the stirring position among measures a stirrer for stirring the mixed solution in the reaction vessel in which the mixed solution is accommodated, the liquid mixture in the reaction vessel passing through the measurement position after stirring is performed by the stirrer A measurement unit, an analysis control unit that drives and controls the reaction container, the washing nozzle, the sample dispensing probe, the reagent dispensing probe, and the stirrer; and measurement of the measurement unit when the sample is a standard sample based on the standard data generated by an arithmetic unit for generating calibration data, the analysis control unit, against the particular reaction vessel cleaning by that previously said cleaning nozzle is performed The washing after the destination washing is performed of whether we pre Symbol washed position n was locked stop at (integer of 2 or more) times stop until the discharge of the reagent to the reaction vessel, the destination When the cleaning is stopped at least once out of the n stops at the reagent discharge position after the cleaning is performed and n times at the cleaning position, the measurement unit performs the previous cleaning. The measurement position between the time when the previous washing is performed and the time when n is stopped at the washing position after the previous washing is performed on the reaction vessel in which the subsequent washing is stopped until n times at the washing position. Each time it passes, the mixture of the standard sample and the reagent in the reaction container is measured to generate a plurality of standard data, and the calculation unit is a plurality of standard data generated by the measurement of the measurement unit At least based on at least one standard data And generating a One calibration data.

The block diagram which shows the structure of the automatic analyzer which concerns on embodiment. The perspective view which shows the structure of the analysis part which concerns on embodiment. The top view which shows each analysis unit of the analysis part which concerns on embodiment. The figure which shows each reaction container and the measurement part which were hold | maintained at the reaction disc which concerns on embodiment. The timing which shows an example of the timing of the operation | movement regarding the reagent dispensing of the 1st and 2nd reagent rack which concerns on embodiment, the reaction disk, the 1st and 2nd reagent dispensing probe, and the 1st and 2nd reagent dispensing arm chart. The figure which shows an example of the structure of the washing | cleaning nozzle which concerns on embodiment, and each stop position which each reaction container stops. The figure which shows an example of the analysis parameter setting screen of the test | inspection item displayed on the display part which concerns on embodiment. The figure which shows an example of the analysis parameter setting screen of the test | inspection item displayed on the display part which concerns on embodiment. The figure which shows an example of the analysis parameter setting screen of the test | inspection item displayed on the display part which concerns on embodiment.

  Hereinafter, embodiments will be described with reference to the drawings.

  FIG. 1 is a block diagram illustrating a configuration of an automatic analyzer according to the embodiment. The automatic analyzer 100 includes an analysis unit 10 that generates a standard data and test data by measuring a mixed solution of a standard sample or test sample for each test item and an analysis reagent for each test item, and an analysis unit. An analysis control unit 50 for driving and controlling each analysis unit related to 10 measurements, a data processing unit 60 for processing standard data and test data generated by the analysis unit 10 to generate calibration data and analysis data, and It has.

  Further, the automatic analyzer 100 includes an output unit 70 that prints out and displays the calibration data and analysis data generated by the data processing unit 60, and an operation unit that performs input for setting analysis parameters of each inspection item. 80, and a system control unit 90 that controls the analysis control unit 50, the data processing unit 60, and the output unit 70 in an integrated manner.

  FIG. 2 is a perspective view showing the configuration of the analysis unit 10. The analysis unit 10 includes a sample container 11 for storing each sample such as a standard sample and a test sample, a sample rack 12 for holding the sample container 11 so as to be movable, and, for example, one reagent system or the like for analyzing each inspection item. A reagent container 13 for storing each of the first to fourth reagents of the four reagent system and a reagent storage 17 for keeping the reagent container 13 cool are provided.

  In addition, the first and second reagent racks 15 and 16 that hold the reagent containers 13 stored in the reagent storage 17 movably, and the reagent containers 13 held in the first and second reagent racks 15 and 16 A reader 18 that reads reagent information from an attached label, and a reaction disk 20 that holds a plurality of reaction vessels 19 arranged in a line on the circumference so as to be rotatable in one direction are provided.

  In addition, the sample dispensing probe 21 for dispensing the sample in the sample container 11 held in the sample rack 12 and discharging it into the reaction container 19 and the sample dispensing probe 21 can be rotated and moved up and down. The first reagent dispenser for dispensing the sample in the reagent container 13 held in the first reagent rack 15 and the reagent in the reagent container 13 held in the first reagent rack 15 to be discharged into the reaction container 19 from which each sample has been discharged. A probe 23 is provided.

  In addition, a first reagent dispensing arm 24 that holds the first reagent dispensing probe 23 so as to be able to rotate and move up and down, and a first stirrer that stirs the mixed solution of the sample and the reagent discharged into the reaction container 19. 25, a first stirring arm 26 that holds the first stirrer 25 so as to be able to rotate and move up and down, and a reagent in the reagent container 13 held in the second reagent rack 16 is aspirated to discharge the sample. A second reagent dispensing probe 27 that performs dispensing to be discharged into the reaction container 19 is provided.

  In addition, a second reagent dispensing arm 28 that holds the second reagent dispensing probe 27 so as to be able to rotate and move up and down, and a second stirrer 29 that agitates each sample and reagent mixture in the reaction container 19 , A second stirring arm 30 that holds the second stirrer 29 so as to be able to rotate and move up and down, a measuring unit 31 that optically measures the liquid mixture in the reaction vessel 19 by measuring light, and a measuring unit 31 And a cleaning nozzle 40 for cleaning the inside of the reaction vessel 19 for which the measurement has been completed.

  Then, the measurement unit 31 irradiates the reaction container 19 with light and detects, for example, absorbance data or absorbance data based on a detection signal that detects light transmitted through the mixed solution containing the standard sample and the test sample in the reaction container 19. Standard data and test data expressed by the amount of change are generated. Then, the generated standard data and test data are output to the data processing unit 60.

  The analysis control unit 50 includes a movement mechanism unit 51 having a movement mechanism that drives each analysis unit of the analysis unit 10, and measurement of each movement mechanism of the movement mechanism unit 51 and the analysis unit 10 based on analysis parameters of each inspection item. And a mechanism control unit 52 that controls the unit 31.

  The moving mechanism unit 51 drives the sample rack 12 to move the sample container 11. Further, the first reagent rack 15 and the second reagent rack 16 are driven independently to rotate the reagent container 13. Further, the reaction disk 20 is driven to rotate and move the reaction vessel 19. Further, the sample dispensing arm 22, the first reagent dispensing arm 24, the second reagent dispensing arm 28, the first stirring arm 26, and the second stirring arm 30 are respectively rotated and driven up and down to sample dispensing. The probe 21, the first reagent dispensing probe 23, the second reagent dispensing probe 27, the first stirring bar 25, and the second stirring bar 29 are rotated and moved up and down, respectively. Further, the cleaning nozzle 40 is moved up and down.

  Further, the moving mechanism unit 51 causes the sample dispensing probe 21 to suck and discharge the sample. Further, the first reagent dispensing probe 23 is caused to suck and discharge the reagent. The second reagent dispensing probe 27 is caused to suck and discharge the reagent. Further, the first stirring bar 25 is allowed to stir the mixed solution in the reaction vessel 19. Further, the second stirring bar 29 is allowed to stir the mixed solution in the reaction vessel 19. Further, the cleaning nozzle 40 is made to clean the inside of the reaction container 19.

  The data processing unit 60 in FIG. 1 processes a standard data and test data output from the measurement unit 31 of the analysis unit 10 to generate calibration data and analysis data for each inspection item, and a calculation unit 61. And a data storage unit 62 for storing the standard data and analysis data generated in step (b).

  The calculation unit 61 generates calibration data representing the relationship between the concentration and activity of each test item component and the standard data from the standard data output from the measurement unit 31 and the standard values preset in the standard sample of the standard data. The generated calibration data is output to the output unit 70 and stored in the data storage unit 62.

  Moreover, the calibration data of the test item corresponding to the test data output from the measurement unit 31 is read from the data storage unit 62, and is expressed as a concentration value or an enzyme activity value from the test data using the read calibration data. Generate analytical data. The generated analysis data is output to the output unit 70 and stored in the data storage unit 62.

  The data storage unit 62 includes a memory device such as a hard disk, and stores calibration data output from the calculation unit 61 for each inspection item. In addition, the analysis data of each inspection item output from the calculation unit 61 is stored for each test sample.

  The output unit 70 includes a printing unit 71 that prints out the calibration data and analysis data output from the calculation unit 61 of the data processing unit 60 and a display unit 72 that displays the output. The printing unit 71 includes a printer or the like, and prints the calibration data and analysis data output from the calculation unit 61 on printer paper or the like according to a preset format.

  The display unit 72 includes a monitor such as a CRT or a liquid crystal panel, and displays calibration data and analysis data output from the calculation unit 61. Also, an analysis parameter setting screen for setting analysis parameters such as the number of rounds for each inspection item, test identification information such as name and ID for identifying the test sample for each test sample, and setting and inspection are necessary. An inspection item setting screen for setting inspection items is displayed.

  The operation unit 80 includes input devices such as a keyboard, a mouse, a button, and a touch key panel. The operation unit 80 is used to set an analysis parameter for each test item, test identification information of a test sample, and test items. Perform input etc.

  The system control unit 90 includes a CPU and a storage circuit, and stores input information such as command signals input by operation from the operation unit 80, analysis parameters for each inspection item, identification information for inspection, and inspection items in the storage circuit. Thereafter, based on the input information, the analysis control unit 50, the data processing unit 60, and the output unit 70 are integrated to control the entire system.

Next, an example of the operation of each analysis unit of the analysis unit 10 will be described with reference to FIGS.
FIG. 3 is a plan view showing each analysis unit of the analysis unit 10. The first reagent rack 15 of the analysis unit 10 is disposed in the reagent storage 17 and holds a plurality of reagent containers 13 arranged on a concentric outer circumference. The second reagent rack 16 is arranged on the inner periphery of the first reagent rack 15 in the reagent store 17, and holds a plurality of reagent containers 13 arranged on the inner circumference of the concentric circle. The first and second reagent racks 15 and 16 are independently rotated by the moving mechanism 51 of the analysis controller 50.

  When the reagent is dispensed by the first reagent dispensing probe 23, the first reagent rack 15 rotates and moves the reagent container 13 containing the reagent to be dispensed at the first reagent aspirating position P1. . Further, the second reagent rack 16 rotates and moves the reagent container 13 containing the reagent to be dispensed at the second reagent aspirating position P2 when the reagent is dispensed by the second reagent dispensing probe 27. Stop.

  FIG. 4 is a view showing each reaction container 19 and the measurement unit 31 held on the reaction disk 20. The reaction disk 20 is rotationally driven by the moving mechanism unit 51. Each reaction container 19 rotates in one direction every cycle time and stops at each stop position such as the first and second reagent discharge positions Pb and Pd and the cleaning position W which are different from those before the movement. Stop at the same stop position every round time which is longer than the cycle time. Here, the reaction disk 20 is rotationally moved in the direction of the arrow R1 every cycle time by the rotational drive of the angle θ, and is, for example, the position of the reaction vessel 19 adjacent in the opposite direction to the R1 direction before the movement. Stop at the position where the pitch has advanced.

  The measurement unit 31 is disposed across the inner and outer peripheries of each reaction container 19 and irradiates each reaction container 19 that passes through the measurement position Pm with each rotation of the reaction disk 20 with light. Then, every time the measurement position Pm passes, the light transmitted through the liquid mixture in each reaction vessel 19 is detected to generate standard data and test data.

  FIG. 5 shows reagent dispensing of the first and second reagent racks 15 and 16, the reaction disk 20, the first and second reagent dispensing probes 23 and 27, and the first and second reagent dispensing arms 24 and 28. It is a timing chart which showed an example of the timing of the operation concerned. Reagent dispensing in this timing chart is performed in one cycle time.

  When the first and second reagent racks 15 and 16 are rotated at the same timing, the reagent container 13 to be dispensed stops at the first and second reagent suction positions P1 and P2. As the reaction disk 20 rotates, each reaction vessel 19 advances one pitch and stops at each position. When the first and second reagent dispensing arms 24 and 28 rotate at the same timing, the first and second reagent dispensing probes 23 and 27 move from the home position, and the first and second reagent aspirating positions P1. , P2 is stopped above the reagent container 13.

  Since the first and second reagent dispensing arms 24 and 28 move downward at the same timing, the first and second reagent dispensing probes 23 and 27 are reagents at the first and second reagent aspirating positions P1 and P2. It stops at a position where the reagent in the container 13 can be aspirated. After the first and second reagent dispensing arms 24 and 28 are stopped, the first and second reagent dispensing probes 23 and 27 remove the reagent in the reagent container 13 at the first and second reagent suction positions P1 and P2. Aspirate at the same time. After the first and second reagent dispensing probes 23 and 27 suck the reagent, the first and second reagent dispensing arms 24 and 28 move upward at the same timing, so that the first and second reagent dispensing The injection probes 23 and 27 stop above the reagent container 13 at the first and second reagent suction positions P1 and P2.

  When the first and second reagent dispensing arms 24 and 28 are rotated at the same timing, the first and second reagent dispensing probes 23 and 27 are moved to the reaction containers 19 at the first and second reagent discharge positions Pb and Pd. Stop at the top. After the first and second reagent dispensing probes 23 and 27 are stopped, the first and second reagent dispensing probes 23 and 27 are placed in the reaction container 19 stopped at the first and second reagent discharge positions Pb and Pd. The reagent is discharged at the same timing. After the first and second reagent dispensing probes 23 and 27 discharge the reagent, the first and second reagent dispensing arms 24 and 28 rotate at the same timing, so that the first and second reagent dispensing probes are turned on. 23 and 27 stop at the home position.

  Thus, by operating the first and second reagent racks 15 and 16, the first and second reagent dispensing arms 24 and 28, and the first and second reagent dispensing probes 23 and 27, respectively, at the same timing. Two reagents can be dispensed in a short time.

FIG. 6 is a diagram showing an example of the configuration of the cleaning nozzle 40 and each stop position at which each reaction vessel 19 stops.
First, the configuration of the cleaning nozzle 40 will be described.
The cleaning nozzle 40 includes, for example, first to fifth cleaning nozzles 41 to 45. The first to fifth cleaning nozzles 41 to 45 are disposed so as to be independently movable in the vertical direction at the first to fifth cleaning positions W1 to W5 at the cleaning position W by the vertical drive of the moving mechanism 51. .

  Each of the first to fifth cleaning nozzles 41 to 45 is stopped at the upper stop position above the reaction disk 20 while the reaction disk 20 is rotating. Then, when the reaction disk 20 is stopped and the reaction container 19 to be cleaned is stopped at each of the first to fifth cleaning positions W1 to W5, the reaction disk 20 moves downward and enters the reaction container 19, and the lower end is Stop and clean at the bottom stop position close to the bottom. After finishing the cleaning, it moves upward and stops at the upper stop position.

  When the reaction vessel 19 to be cleaned is stopped at the first cleaning position W1, the first cleaning nozzle 41 moves downward and stops at the lower stop position. Then, cleaning is performed by sucking the mixed liquid in the reaction vessel 19. When the reaction container 19 other than the object to be cleaned is stopped at the first cleaning position W1, the cleaning is stopped at the upper stop position.

  When the reaction vessel 19 cleaned at the first cleaning position W1 is stopped at the second cleaning position W2, the second cleaning nozzle 42 moves downward and stops at the lower stop position. Then, cleaning is performed by discharging a first cleaning liquid such as an alkaline cleaning liquid and washing the discharged first cleaning liquid in order to wash off the mixed liquid remaining in the reaction container 19. Further, when the reaction containers 19 other than the reaction container 19 that has sucked the mixed liquid at the first cleaning position W1 are stopped at the second cleaning position W2, the cleaning is stopped at the upper stop position.

  The third cleaning nozzle 43 moves downward and stops at the lower stop position when the reaction vessel 19 cleaned at the second cleaning position W2 is stopped at the third cleaning position W3. Then, cleaning is performed by discharging a second cleaning liquid such as an acidic cleaning liquid and suctioning the discharged second cleaning liquid in order to wash out the mixed liquid that cannot be removed by the first cleaning liquid in the reaction vessel 19. Further, when the reaction containers 19 other than the reaction container 19 cleaned at the second cleaning position W2 are stopped at the third cleaning position W3, the cleaning is stopped at the upper stop position.

  When the reaction vessel 19 cleaned at the third cleaning position W3 is stopped at the fourth cleaning position W4, the fourth cleaning nozzle 44 moves downward and stops at the lower stop position. Then, cleaning is performed by discharging cleaning water for washing away the second cleaning liquid in the reaction container 19 and suction of the discharged cleaning water. Further, when the reaction containers 19 other than the reaction container 19 cleaned at the third cleaning position W3 are stopped at the fourth cleaning position W4, the cleaning is stopped at the upper stop position.

  The fifth cleaning nozzle 45 moves downward and stops at the lower stop position when the reaction vessel 19 cleaned at the fourth cleaning position W4 is stopped at the fifth cleaning position W5. Then, cleaning is performed by suction of the cleaning water remaining in the reaction vessel 19. Further, when the reaction containers 19 other than the reaction container 19 cleaned at the fourth cleaning position W4 are stopped at the fifth cleaning position W5, the cleaning is stopped at the upper stop position.

Next, an example of each stop position at which each reaction container 19 stops each time the reaction disk 20 rotates, and the operation of dispensing and stirring the sample and reagent at each stop position will be described.
The mechanism control unit 52 of the analysis control unit 50 finishes cleaning and stops at the fifth cleaning position W5 of the cleaning position W with the fifth cleaning nozzle 45 of the cleaning nozzle 40 stopped at the upper stop position. The starting position is the starting position, and the reaction container 19 at the starting position is determined for analysis of the inspection item set for each test sample. The reaction container 19 determined for analysis of the inspection item is stopped in order of the sample discharge position Pa, the first reagent discharge position Pb, the first stirring position Pc, the second reagent discharge position Pd, the second stirring position Pe, and the cleaning position W. To do.

  Here, when 1 is set as the number of rounds of the analysis parameter by the input from the operation unit 80, the mechanism control unit 52 is for analyzing the inspection item stopped at the cleaning position W for the first time after the cleaning is performed. The reaction vessel 19 is cleaned. If n (an integer greater than or equal to 2) is set as the number of rounds, the cleaning of the reaction container 19 for analysis of test items is stopped until the nth stop at the cleaning position W after the cleaning is performed. For this reason, the reaction container 19 for analysis of the test item is subjected to the sample discharge position Pa, the first reagent discharge position Pb, the first stirring position Pc, and the second reagent during the period from the cleaning to the next cleaning. Stop n times at each stop position of the discharge position Pd and the second stirring position Pe.

  Therefore, the sample can be discharged to the reaction container 19 for analyzing the inspection item at the sample discharge position Pa at the timing of n times, and the sample can be discharged at the timing of at least one of the n times. In addition, the reagent can be discharged to the reaction container 19 for analyzing the test item at the first reagent discharge position Pb at the timing of n times, and the reagent can be discharged at least at the timing of the n times. . In addition, the liquid mixture in the reaction container 19 for analysis of the inspection item can be stirred at n times at the first stirring position Pc, and the liquid mixture is stirred at at least one of the n times. Can do. In addition, the reagent can be discharged into the reaction container 19 for analysis of the test item at the second reagent discharge position Pd at the timing of n times, and the reagent can be discharged at least at the timing of the n times. . In addition, the liquid mixture in the reaction container 19 for analysis of the inspection item can be stirred at n times at the second stirring position Pe, and the liquid mixture is stirred at at least one of the n times. Can do.

  When n is set as the number of rounds, the reaction container 19 determined for analysis of the inspection item is stopped at each stop position as the first round until it stops at the same stop position as the start position after one round time. To do.

  In the first cycle of the first round, the reaction container 19 for inspection item analysis moves from the fifth cleaning position W5 and stops at a stop position advanced by one pitch. Next, in the a-th cycle after a predetermined cycle time has elapsed from the first cycle, the cleaning is performed and then stopped at the sample discharge position Pa for the first time. When the discharge of the sample is set at the timing of the first round that is at least one of the n times, the sample dispensing probe 21 is removed from the sample container 11 held in the sample rack 12. The sample is sucked and discharged into the reaction container 19 at the sample discharge position Pa.

  The reaction container 19 stopped at the sample discharge position Pa in the a cycle in the b cycle when one cycle time has elapsed from the a cycle is stopped at the first reagent discharge position Pb after the cleaning is performed. When the reagent discharge from the first reagent discharge position Pb is set at the timing of the first round, which is at least one of the n timings, the first reagent dispensing probe 23 The reagent is sucked from the reagent container 13 at the first reagent suction position P1 held in the one reagent rack 15, and discharged into the reaction container 19 at the first reagent discharge position Pb.

  In the c-th cycle after one cycle time has elapsed from the b-th cycle, the reaction vessel 19 stopped at the first reagent discharge position Pb in the b-th cycle is stopped for the first time at the first stirring position Pc after being washed. And when stirring of the liquid mixture from the 1st stirring position Pc is set by the timing of the 1st round which is the timing of at least 1 time among n times, the 1st stirring element 25 is 1st stirring. The mixed solution of the sample and the reagent in the reaction container 19 at the position Pc is stirred.

  In the d cycle when a predetermined cycle time has elapsed from the c cycle, the reaction vessel 19 stopped at the first stirring position Pc in the c cycle is stopped for the first time at the second reagent discharge position Pd after being washed. . When the reagent discharge from the second reagent discharge position Pd is set at the timing of the first round, which is at least one of the n timings, the second reagent dispensing probe 27 The reagent is sucked from the reagent container 13 at the second reagent suction position P2 held in the two reagent rack 16, and discharged into the reaction container 19 at the second reagent discharge position Pd.

  In the e cycle when one cycle time elapses from the d cycle, the reaction vessel 19 stopped at the second reagent discharge position Pd in the d cycle is stopped for the first time at the second stirring position Pe after the cleaning is performed. And when stirring of the liquid mixture from the 2nd stirring position Pe is set at the timing of the 1st round which is at least 1 timing among the timings of n times, the 2nd stirring element 29 is the 2nd stirring. The mixed solution in the reaction vessel 19 at the position Pe is stirred.

  In the f-th cycle after the elapse of a predetermined cycle time from the e-th cycle, the reaction vessel 19 stopped at the second stirring position Pe in the e-th cycle is stopped for the first time at the first cleaning position W1 after being cleaned. The first cleaning nozzle 41 stops the cleaning of the reaction vessel 19 at the first cleaning position W1.

  In the (f + 1) th cycle after one cycle time has passed since the fth cycle, the reaction vessel 19 stopped at the first cleaning position W1 in the fth cycle stops for the first time at the second cleaning position W2. The second cleaning nozzle 42 stops the cleaning of the reaction vessel 19 at the second cleaning position W2.

  In the (f + 2) cycle when one cycle time has elapsed from the (f + 1) cycle, the reaction vessel 19 stopped at the second cleaning position W2 in the (f + 1) cycle stops at the third cleaning position W3 for the first time. The third cleaning nozzle 43 stops the cleaning of the reaction vessel 19 at the third cleaning position W3.

  In the (f + 3) cycle when one cycle time has elapsed from the (f + 2) cycle, the reaction vessel 19 stopped at the third cleaning position W3 in the (f + 2) cycle stops at the fourth cleaning position W4 for the first time. The fourth cleaning nozzle 44 stops the cleaning of the reaction vessel 19 at the fourth cleaning position W4.

  In the (f + 4) cycle when one cycle time has elapsed from the (f + 3) cycle, the reaction vessel 19 stopped at the fourth cleaning position W4 in the (f + 3) cycle stops at the fifth cleaning position W5 for the first time. The fifth cleaning nozzle 45 stops the cleaning of the reaction container 19 at the fifth cleaning position W5.

  The inspection item analysis reaction vessel 19 stopped at the fifth cleaning position W5 in the (f + 4) cycle has been cleaned and the fifth cleaning position W5 with the fifth cleaning nozzle 45 stopped at the upper stop position. The position at the time of stopping at is the end position of the first round.

  When n is 2, the end position of the first round is the start position of the second round. Then, the target reaction vessel 19 stops at the sample discharge position Pa, the first and second reagent discharge positions Pb and Pd, and the first and second stirring positions Pc and Pe during the second round.

  The reaction container 19 for analysis of the inspection item is cleaned by the cleaning nozzle 40 when stopped for the second time at the cleaning position, and the cleaning is finished with the fifth cleaning nozzle 45 stopped at the upper stop position. The position at the time of stopping at the fifth cleaning position W5 is set as the end position of the second round. And it is determined as the reaction container 19 for the next inspection item analysis.

  When the sample is a standard sample, the measurement unit 31 checks each time the measurement position Pm passes from the time when the reagent is discharged to the reaction container 19 for analysis of the test item until the nth stop at the cleaning position W. A plurality of standard data is generated by measuring a mixed solution of the standard sample and the reagent in the reaction container 19 for analysis. The calculation unit 61 of the data processing unit 60 generates at least one calibration data based on the standard data at at least one timing among the plurality of standard data generated by the measurement unit 31.

  In addition, when the sample is a test sample, the measurement unit 31 is configured to perform measurement every time the measurement position Pm passes from when the reagent is discharged to the reaction container 19 for analyzing the test item until the nth stop at the cleaning position W. In addition, a plurality of test data are generated by measuring the test sample and the mixed solution of the reagent in the reaction container 19 for analyzing the test item. The calculation unit 61 generates analysis data based on at least one test data and at least one calibration data among a plurality of test data generated by the measurement unit 31.

  Hereinafter, an example of the operation of the automatic analyzer 100 will be described with reference to FIGS. 1 to 9. In the following, the number of first to third reagents larger than the number of the first and second reagent dispensing probes 23 and 27 and the reaction time up to the n-th round longer than the reaction time in the first round are required. For example, an operation for analysis of inspection items A, B, and C will be described. When an input for setting analysis parameters for each of the inspection items A, B, and C is performed from the operation unit 80, an analysis parameter setting screen is displayed on the display unit 72 of the output unit 70.

  FIG. 7 is a diagram illustrating an example of an analysis parameter setting screen for the inspection item A displayed on the display unit 72. The analysis parameter setting screen 73 includes an “item” column for setting the item name of the inspection item A and a “round” column for setting the number of rounds. In addition, a column of “Reaction time” for setting the reaction time of the sample and the reagent of the inspection item A, and “Sample parameter”, “Reagent parameter”, and “Agitation parameter” for setting the sample, reagent, and stirring parameters ”Column.

  The analysis parameter setting screen 73 includes a “Reaction sequence” column in which a timing chart of sample and reagent dispensing is displayed according to settings in the “round”, “reaction time”, and “reagent parameter” columns. Is done. In each column, each analysis parameter set by input from the operation unit 80 is displayed.

  In the “item” column, “A” which is the item name of the inspection item A is displayed. In the “round” column, “2” indicating that the number of rounds is 2 is displayed. In the “reaction time” column, “Tw2” indicating that the reaction time for analyzing the inspection item A is the time Tw2 is displayed. With this setting, when the time Tw2 has elapsed since stopping at the first reagent discharge position Pb in the first round, the measurement unit 31 ends the measurement, and the output unit 70 outputs the analysis data of the test item A.

  In the “sample parameter” column, the “sample / diluent” column for setting the test sample and diluent to be discharged to the reaction container 19 and the amount of the test sample and diluent to be discharged to the reaction container 19 are displayed. It is composed of a “discharge amount (μL)” column for setting and a “discharge round” column for setting the timing of discharging the test sample and the diluted solution to the reaction container 19.

  In the “sample / diluent” column, “Sa” indicating that the test sample for analysis of the test item A is discharged into the reaction container 19 for analysis of the test item A is displayed. Further, “Sr” and “Sd” are displayed in the reaction container 19 to indicate that the test sample and the diluted solution for the re-examination of the inspection item A are discharged.

  In the “discharge amount (μL)” column, “5” indicating that 5 μL of the test sample for analysis of the test item A is discharged into the reaction container 19 for analysis of the test item A is displayed. Further, “10” indicating that 10 μL of the test sample for retesting of the inspection item A is discharged into the reaction container 19 is displayed, and “100” indicating that 100 μL of the diluted solution is discharged is displayed.

  In the “discharge round” column, “1” indicating that the test sample for analysis of the test item A is discharged into the reaction container 19 for analysis of the test item A that stops at the sample discharge position Pa in the first round is displayed. Has been. Further, the timing for discharging the test sample for retesting of the test item A and the diluent is not set in the reaction container 19 and is blank.

  In this way, when 2 is set as the number of rounds, the sample can be discharged into the reaction container 19 for analysis of the inspection item A at the sample discharge position Pa at two timings, and one of the two timings. It is possible to perform setting so that the test sample for analysis of the test item A is discharged in the first round that is the second timing.

  When 2 is set as the number of rounds, two “Sa” indicating that the test sample is to be discharged is set in the “sample / diluent” column in the “sample parameter” column, and “discharge round” is set. "1" and "2" are set in the column, respectively, so that the sample can be discharged at two timings of the first round and the second round.

  The “reagent parameter” column is a “reagent” column for setting which of the first to fourth reagents is used, and for example, a reagent name for identifying each first to fourth reagent is set. “Reagent name” column, “Discharge amount (μL)” column for setting the amount of reagent to be discharged to the reaction container 19, “Discharge position” for setting the reagent discharge position for discharging the reagent to the reaction container 19 Column, and a “discharge round” column for setting the timing of discharging the reagent to the reaction container 19.

  In the “reagent” column, “R1” indicating that the reagent of the test item A is composed of first to third reagents of the three reagent system and is the first reagent to be dispensed first, and the first reagent is dispensed. “R2” indicating that the second reagent is dispensed after being dispensed and “R3” indicating that the second reagent is dispensed after the second reagent is dispensed are displayed.

  In the “reagent name” column, “A1” indicating that the reagent name of the first reagent used for the analysis of the test item A is the reagent A1 is displayed, and the reagent name of the second reagent is the reagent A2. “A2” is displayed. In addition, “A3” indicating that the reagent name of the third reagent is reagent A3 is displayed.

  In the “discharge amount (μL)” column, “100” indicating that the reagent amount of the first reagent discharged into the reaction container 19 for analysis of the test item A is 100 μL is displayed. Further, “50” indicating that the amount of the second reagent discharged to the reaction container 19 for analysis of the test item A is 50 μL is displayed. Further, “20” indicating that the reagent amount of the third reagent discharged to the reaction container 19 for analysis of the test item A is 20 μL is displayed.

  In the “discharge position” and “discharge round” columns, “Pb” indicating that the first reagent is discharged into the reaction container 19 for analysis of the test item A that stops at the first reagent discharge position Pb in the first round. And “1” are displayed. Further, “Pd” and “1” indicating that the second reagent is to be discharged into the reaction container 19 for analysis of the test item A that stops at the second reagent discharge position Pd in the first round are displayed. In addition, “Pd” and “2” indicating that the third reagent is discharged into the reaction container 19 for analysis of the test item A that stops at the second reagent discharge position Pd in the second round are displayed.

  As described above, when 2 is set as the number of rounds, the reagent can be discharged into the reaction container 19 for analysis of the test item A at the first reagent discharge position Pb at two times. The first reagent can be set to be discharged in the first round which is the first timing. In addition, the reagent can be discharged into the reaction container 19 for analysis of the test item A at the second reagent discharge position Pd at two timings, and the second is added to the first round which is the first timing of the two times. It is possible to perform setting so that the reagent is discharged and the third reagent is discharged in the second round, which is the second timing.

  The “stirring parameter” column includes a “stirring position” column for setting a stirring position for stirring the mixed solution and a “discharge round” column for setting timing for stirring the mixed solution. In the “stirring position” and “stirring round” columns, “Pc” indicating that the mixed liquid in the reaction container 19 for analysis of the inspection item A that stops at the first stirring position Pc in the first round is stirred. And “1” are displayed. In addition, “Pe” and “1” indicating that the liquid mixture in the reaction container 19 for the inspection item A analysis that stops at the second stirring position Pe in the first round is displayed. In addition, “Pe” and “2” indicating that the mixed solution in the reaction container 19 for the inspection item A analysis that stops at the second stirring position Pe is stirred in the second round are displayed.

  Thus, when 2 is set as the number of rounds, the liquid mixture in the reaction container 19 for analysis of the test item A can be stirred at the first stirring position Pc at two timings. The setting which stirs a liquid mixture in the 1st round which is the timing of the 1st time of them can be performed. In addition, the liquid mixture in the reaction container 19 for analysis of the inspection item A can be stirred at the second stirring position Pe at the timing of two times, and the setting for stirring in the first and second rounds can be performed.

  The timing chart displayed in the “Reaction sequence” column is composed of two arrows 1R and 2R indicating the first and second rounds corresponding to the number of rounds set in the “Round” column. Also, the time from when the reaction container 19 for analysis of the test item A stops for the first time at the first reagent discharge position Pb in the first round to when it stops for the first time at the second reagent discharge position Pd in the first round. “T1” indicating T1, “T2” indicating a time T2 until the second round is stopped at the first reagent discharge position Pb, and “T2” is stopped at the second reagent discharge position Pd in the second round. “T3” indicating the time T3 until the start, “Tw2” indicating the time Tw2 until the second round is stopped at the first cleaning position W1 of the cleaning position W, and “Tw2” as the reaction time. Consists of arrows pointing to the right.

  Further, the reaction container 19 for analysis of the test item A is constituted by “Sa” indicating that the test sample for analysis is discharged when stopped at the sample discharge position Pa in the first round and an arrow pointing downward. Is done.

  In addition, when the reaction container 19 for analysis of the test item A stops at the first reagent discharge position Pb in the first round, “R1” and “100 μL” indicating that 100 μL of the first reagent is discharged are directed downward. Arrows indicating that 50 μL of the second reagent is to be discharged when stopped at the second reagent discharge position Pd in the first round, and an arrow pointing downward in the second round, It is composed of “R3” and “20 μL” indicating that 20 μL of the third reagent is discharged when stopped at the two reagent discharge position Pd, and an arrow pointing downward.

  In addition, by operating the mouse of the operation unit 80 and moving, for example, the arrow “R2” displayed in the “Reaction sequence” column to the first reagent discharge position Pb in the second round, “discharge round” and The second reagent discharge positions “1” and “Pd” displayed in the “discharge position” column are changed to the first reagent discharge position Pb in the second round, and “2” and “Pb” are displayed. Can be made. Further, by operating the mouse and keyboard of the operation unit 80 and changing the reagent amount “100 μL” of the first reagent displayed in the “Reaction sequence” column to “90 μL”, for example, the “reagent amount ( It is possible to change and set “100” displayed in the “μL)” column to “90” to discharge 90 μL of the first reagent.

  In this way, by displaying the reagent dispensing timing chart on the display unit 72, the operator can easily grasp the entire reaction such as the dispensing timing, reagent amount, and reaction time of the reagent of the test item A. Can do.

  The mechanism control unit 52 of the analysis control unit 50 controls the moving mechanism unit 51 and the measurement unit 31 based on the analysis parameters displayed on the analysis parameter setting screen 73 to operate each analysis unit of the analysis unit 10.

  In the first round, the sample dispensing probe 21 is the first inspection item that stops at the sample discharge position Pa after being cleaned by the fifth cleaning nozzle 45 of the cleaning nozzle 40 at the fifth cleaning position W5 of the cleaning position W. 5 μL of the test sample is discharged into the reaction vessel 19 for analysis. The first reagent dispensing probe 23 discharges 100 μL of the first reagent into the reaction container 19 for analysis of the test item A in which the test sample to be stopped for the first time is discharged at the first reagent discharge position Pb. Moreover, the 1st stirring element 25 stirs the liquid mixture in the reaction container 19 for the test item A analysis stopped at the 1st stirring position Pc for the first time. The second reagent dispensing probe 27 discharges 50 μL of the second reagent into the reaction container 19 for analysis of the test item A that stops at the first time at the second reagent discharge position Pd. The second stirrer 29 stirs the mixed solution in the reaction container 19 for the inspection item A that stops for the first time at the second stirring position Pe. Further, the cleaning nozzle 40 stops the cleaning of the reaction container 19 for analysis of the inspection item A that stops at the cleaning position W for the first time.

  Thus, by setting 2 as the number of rounds, by stopping the cleaning of the reaction container 19 for the test item A analysis that stops at the cleaning position W in the first round, the test sample, the first reagent, and the first reagent The second reaction, which is a reaction of the mixed solution with the two reagents, can be continued without being completed in the first round.

  In the second round, the sample dispensing probe 21 stops the discharge of the test sample into the reaction container 19 for the inspection item A analysis that stops at the second time at the sample discharge position Pa. In addition, the first reagent dispensing probe 23 stops the discharge of the reagent into the reaction container 19 for analysis of the test item A, which stops for the second time at the first reagent discharge position Pb. Moreover, the 1st stirring element 25 stops the stirring in the reaction container 19 for the test | inspection item A analysis stopped for the 2nd time in the 1st stirring position Pc. The second reagent dispensing probe 27 discharges 20 μL of the third reagent into the reaction container 19 for analysis of the test item A that stops at the second reagent discharge position Pd for the second time. Moreover, the 2nd stirring element 29 stirs the liquid mixture in the reaction container 19 for the test item A analysis stopped at the 2nd stirring position Pe for the 2nd time. In addition, the cleaning nozzle 40 cleans the inside of the reaction container 19 for analysis of the inspection item A that stops at the cleaning position W for the second time after the cleaning is performed.

  In this way, by setting the reaction time in the column of “Reaction time” on the analysis parameter setting screen 73, the third reaction, which is the reaction of the mixed solution of the test sample and the first to third reagents, can be changed. Even when the reaction of 2 is continued until the second round, it can be performed without shortening the time.

  Further, by setting 2 as the number of rounds, the third reagent is dispensed in the second round using the second reagent dispensing probe 27 without providing a dedicated reagent dispensing probe for dispensing the third reagent. Can be dispensed. As a result, a larger number of first to third reagents than the number of the first and second reagent dispensing probes 23 and 27 are replaced with the first and second reagent dispensing probes 23 and 27, the first and second reagents. The reagent dispensing arms 24 and 28 and the moving mechanisms that drive the first and second reagent dispensing arms 24 and 28, respectively, can be used for dispensing, thereby preventing the automatic analyzer 100 from becoming complicated and large. be able to.

  When the reagent of the test item A is, for example, the first to fourth reagents, the cleaning of the reaction container 19 for the test item A that stops at the cleaning position W is stopped for the second time in the second round, for example, the third reagent The fourth reagent may be dispensed by the first or second reagent dispensing probes 23 and 27 in a round.

  The measurement unit 31 is a reaction container for analysis of the inspection item A that passes through the measurement position Pm every cycle time from the first stop at the first stirring position Pc to the second stop at the cleaning position W. The liquid mixture in 19 is measured to generate absorbance data. Then, the calculation unit 61 of the data processing unit 60 generates analysis data of the inspection item A based on the absorbance data generated by the measurement unit 31. The output unit 70 outputs the analysis data of the inspection item A when the reaction container 19 for analysis of the inspection item A stops at the first cleaning position W1.

  In this way, by setting the reaction time in the column of “Reaction time” on the analysis parameter setting screen 73, the inspection item A is set at a timing corresponding to the reaction time earlier than the end timing of the second round as the final round. Analysis data can be output.

  In the case of a multi-assay that analyzes two test items D and E using the first reagent for analysis of the test item D and the second reagent for analysis of the test item E, for example, 2 is set as the number of rounds. After the first reagent is discharged into the reaction container 19 in which the test items D and E to be tested for the first stop at the first reagent discharge position Pb are discharged in one round, and then at the first stirring position Pc. The mixed solution of the test sample and the first reagent in the reaction container 19 stopped at the first time is stirred. Next, after the second reagent is discharged into the reaction container 19 for inspection items D and E which are stopped at the first or second reagent discharge positions Pb and Pd in the second round, the first or second stirring position Pc , Pe, the test sample in the reaction vessel 19 stopped for the second time and the mixed solution of the first and second reagents are stirred. Then, the analysis data of the inspection item D is generated by the measurement after the first reagent is discharged and stirred until the second reagent is discharged, and after the second reagent is discharged, the analysis data is stirred and then washed. You may implement so that the analysis data of the test | inspection item E may be produced | generated by the measurement until it stops at the position W. FIG.

  FIG. 8 is a diagram illustrating an example of an analysis parameter setting screen for the inspection item B displayed on the display unit 72. The analysis parameter setting screen 74 includes the same fields as the analysis parameter setting screen 73 in FIG.

  In the “item” column, “B” which is the item name of the inspection item B is displayed. In the “round” column, “5” indicating that the number of rounds is 5 is displayed. In the “reaction time” column, “T9” indicating that the reaction time of the mixed solution for analyzing the inspection item B is time T9 is displayed. With this setting, when time T9 elapses after stopping at the first reagent discharge position Pb in the first round, the measurement unit 31 ends the measurement, and the output unit 70 outputs the analysis data of the test item B.

  In the “sample / diluent” column of the “sample parameter” column, “Sa” indicating that the test sample for analysis of the test item B is discharged into the reaction container 19 is displayed. In addition, “Sr” and “Sd” are displayed in the reaction container 19 to indicate that a test sample and a diluted solution for retesting the inspection item B are discharged.

  In the “discharge amount (μL)” column, “2” indicating that 2 μL of the test sample for analysis of the test item B is discharged into the reaction container 19 for analysis of the test item B is displayed. Further, “10” indicating that 10 μL of the test sample for retesting of the inspection item B is discharged into the reaction container 19 is displayed, and “100” indicating that 100 μL of the diluted solution is discharged is displayed.

  In the “discharge round” column, “1” indicating that the test sample for analysis of the test item B is discharged into the reaction container 19 for analysis of the test item B that stops at the sample discharge position Pa in the first round is displayed. It is displayed. Moreover, since the timing which discharges the test sample for the reexamination of the test item B and the dilution liquid in the reaction container 19 is not set, it is blank.

  As described above, when 5 is set as the number of rounds, the sample can be discharged to the reaction container 19 for analysis of the inspection item B at the sample discharge position Pa at the timing of 5 times, and one of the 5 timings. It is possible to make a setting for discharging the test sample for analysis of the inspection item B in the first round which is the timing of the second time.

  In the “reagent” column of the “reagent parameter” column, “R1” indicating that the reagent of the test item B includes the first to third reagents of the three reagent system and is the first reagent to be dispensed first. “R2” indicating that the second reagent is dispensed after the first reagent is dispensed, and “R3” indicating that the second reagent is dispensed after the second reagent is dispensed. It is displayed.

  In the “reagent name” column, “B1” indicating that the reagent name of the first reagent used for the analysis of the test item B is reagent B1 is displayed, and the reagent name of the second reagent is reagent B2. “B2” is displayed. In addition, “B3” indicating that the reagent name of the third reagent is reagent B3 is displayed.

  In “discharge amount (μL)”, “100” indicating that the reagent amount of the first reagent discharged into the reaction container 19 for analysis of the test item B is 100 μL is displayed. Further, “60” indicating that the amount of the second reagent discharged to the reaction container 19 for analysis of the test item B is 60 μL is displayed. Further, “20” indicating that the reagent amount of the third reagent discharged to the reaction container 19 for analysis of the test item B is 20 μL is displayed.

  In the “discharge position” and “discharge round” fields, “Pb” indicating that the first reagent is discharged into the reaction container 19 for analysis of the test item B that stops at the first reagent discharge position Pb in the first round. And “1” are displayed. In addition, “Pb” and “2” indicating that the second reagent is discharged into the reaction container 19 for analysis of the inspection item B that stops at the first reagent discharge position Pb in the second round are displayed. In addition, “Pd” and “4” indicating that the third reagent is discharged into the reaction container 19 for analysis of the test item B that stops at the second reagent discharge position Pd in the fourth round are displayed.

  Thus, when 5 is set as the number of rounds, the reagent can be discharged into the reaction container 19 for analysis of the test item B at the first reagent discharge position Pb at the timing of 5 times. The first reagent can be discharged in the first round, which is the first timing, and the second reagent can be discharged in the second round, which is the second timing. In addition, the reagent can be discharged into the reaction container 19 for analysis of the inspection item B at the second reagent discharge position Pd at the timing of five times, and the third time is set at the fourth round which is the fourth timing among the five times. Settings for discharging the reagent can be made.

  In the “stirring position” and “stirring round” columns in the “stirring parameter” column, the liquid mixture in the reaction vessel 19 for the inspection item B analysis that stops at the first stirring position Pc in the first round is stirred. “Pc” and “1” are displayed. In addition, “Pc” and “2” indicating that the liquid mixture in the reaction container 19 for the inspection item B analysis that stops at the first stirring position Pc is stirred in the second round are displayed. In addition, “Pe” and “4” indicating that the mixed liquid in the reaction container 19 for the inspection item B analysis that stops at the second stirring position Pc is stirred in the fourth round are displayed.

  As described above, when 5 is set as the number of rounds, the liquid mixture in the reaction container 19 for analysis of the inspection item B can be stirred at the first stirring position Pc at the timing of 5 times. The setting which stirs a liquid mixture in the 1st and 2nd round which is the timing of the 1st time and the 2nd time of them can be performed. In addition, the liquid mixture in the reaction vessel 19 for analysis of the inspection item B can be stirred at the second stirring position Pe at the timing of five times, and the mixture is stirred at the fourth round, which is the fourth timing among the five timings. Can be set.

  The timing chart displayed in the “Reaction sequence” column includes five arrows 1R to 5R indicating the first to fifth rounds corresponding to the number of rounds set in the “Round” column. Also, the time from when the reaction container 19 for analysis of the test item B stops for the first time at the first reagent discharge position Pb in the first round until it stops for the first time at the second reagent discharge position Pd in the first round. “T1” indicating T1 and “T2” and “T3” indicating times T2 and T3 until the second stop at the first and second reagent discharge positions Pb and Pd are displayed in the second round.

  In addition, after the reaction container 19 for analysis of the test item B stops for the first time at the first reagent discharge position Pb, it stops until the third stop at the first and second reagent discharge positions Pb and Pd in the third round. “T4” and “T5” indicating times T4 and T5, and “T6” and “T7” indicating times T6 and T7 until the fourth round stops at the first and second reagent discharge positions Pb and Pd in the fourth round. ”,“ T8 ”and“ T9 ”indicating times T8 and T9 until the fifth stop at the first and second reagent discharge positions Pb and Pd in the fifth round, and“ T9 ”as the reaction time. Consists of arrows indicating directions.

  In addition, the reaction container 19 for analysis of the test item B is constituted by “Sa” indicating that the test sample for analysis is discharged when stopped at the sample discharge position Pa in the first round, and an arrow pointing downward. Is done.

  In addition, when the reaction container 19 for analysis of the test item B stops at the first reagent discharge position Pb in the first round, it indicates that 100 μL of the first reagent is discharged “R1”, “100 μL”, and the downward direction. An arrow indicating the discharge of 60 μL of the second reagent when stopped at the first reagent discharge position Pb in the second round, an arrow pointing downward with “R2”, “60 μL” and the fourth in the fourth round It is composed of “R3” and “20 μL” indicating that 20 μL of the third reagent is discharged when stopped at the two reagent discharge position Pd, and an arrow pointing downward.

  The mechanism control unit 52 controls the moving mechanism unit 51 based on the analysis parameter displayed on the analysis parameter setting screen 74 to operate each analysis unit of the analysis unit 10.

  In the first round, the sample dispensing probe 21 is cleaned by the fifth cleaning nozzle 45 at the fifth cleaning position W5 and then stopped for the first time at the sample discharge position Pa. 2 μL of the test sample is discharged into the inside. Further, the first reagent dispensing probe 23 discharges 100 μL of the first reagent into the reaction container 19 for analysis of the test item B that stops for the first time at the first reagent discharge position Pb. Moreover, the 1st stirring element 25 stirs the liquid mixture in the reaction container 19 for the test | inspection item B analysis stopped for the 1st time in the 1st stirring position Pc. In addition, the second reagent dispensing probe 27 stops the discharge of the reagent into the reaction container 19 for analysis of the test item B, which stops for the first time at the second reagent discharge position Pd, and the second stirrer 29 performs the second stirring. Stirring in the reaction vessel 19 for analysis of the inspection item B that stops at the position Pe for the first time is stopped. Further, the cleaning nozzle 40 stops the cleaning of the reaction container 19 for analysis of the inspection item B that stops at the cleaning position W for the first time.

  In this way, the first reaction, which is the reaction of the mixed solution of the test sample and the first reagent, is stopped by stopping the cleaning of the reaction container 19 for analysis of the test item B that stops at the cleaning position W in the first round. Can be continued.

  In the second round, the sample dispensing probe 21 stops the discharge of the test sample into the reaction container 19 for the inspection item B analysis that stops at the second time at the sample discharge position Pa. Further, the first reagent dispensing probe 23 discharges 60 μL of the second reagent into the reaction container 19 for analysis of the test item B that stops for the second time at the first reagent discharge position Pb. Moreover, the 1st stirring element 25 stirs the liquid mixture in the reaction container 19 for the test item B analysis stopped at the 2nd time in the 1st stirring position Pc. The second reagent dispensing probe 27, the second stirrer 29, and the washing nozzle 40 are used for the reaction for the test item B that stops at the second reagent discharge position Pd, the second stirring position Pe, and the washing position W for the second time. The operation of discharging, stirring and washing the reagent to the container 19 is stopped.

  In this way, by stopping the cleaning of the reaction container 19 for analysis of the test item B that stops at the cleaning position W in the second round, the reaction of the mixed liquid of the test sample and the first and second reagents is stopped. The reaction of 2 can be continued.

  In the third round, the sample dispensing probe 21, the first reagent dispensing probe 23, the first stirrer 25, the second reagent dispensing probe 27, the second stirrer 29, and the washing nozzle 40 are arranged at the sample discharge position Pa, the first 1 sample discharge position Pb, 1st stirring position Pc, 2nd reagent discharge position Pd, 2nd stirring position Pe, and discharge of the sample and the reagent with respect to the reaction container 19 for the test item B analysis stopped at the 3rd time, Stop the stirring and washing operations.

  Thus, by stopping the cleaning of the reaction container 19 for analysis of the inspection item B that stops at the cleaning position W in the third round, the reaction of the mixed liquid of the test sample and the first and second reagents is stopped. The reaction of 2 can be continued further.

  In the fourth round, the sample dispensing probe 21, the first reagent dispensing probe 23, and the first stirring bar 25 are inspected for the fourth time at the sample ejection position Pa, the first reagent ejection position Pb, and the first stirring position Pc. Item B Discharge of sample and reagent to the reaction vessel 19 for analysis and the operation of stirring are stopped. Further, the second reagent dispensing probe 27 discharges 20 μL of the third reagent into the reaction container 19 for analysis of the test item B that stops for the fourth time at the second reagent discharge position Pd. Moreover, the 2nd stirring element 29 stirs the liquid mixture in the reaction container 19 for the test item B analysis stopped at the 4th time in the 2nd stirring position Pe. Further, the cleaning nozzle 40 stops the cleaning of the reaction container 19 for analysis of the inspection item B, which stops at the cleaning position W for the fourth time.

  In this way, by stopping the cleaning of the reaction container 19 for analysis of the inspection item B that stops at the cleaning position W in the fourth round, the reaction of the mixed liquid of the test sample and the first to third reagents is stopped. The reaction of 3 can be continued.

  In addition, the third reagent can be dispensed using the second reagent dispensing probe 27 in the fourth round without providing a dedicated analysis unit for dispensing the third reagent. As a result, a larger number of first to third reagents than the number of the first and second reagent dispensing probes 23 and 27 are replaced with the first and second reagent dispensing probes 23 and 27, the first and second reagents. The reagent dispensing arms 24 and 28 and the moving mechanisms that drive the first and second reagent dispensing arms 24 and 28, respectively, can be used for dispensing, thereby preventing the automatic analyzer 100 from becoming complicated and large. be able to.

  In the fifth round, the sample dispensing probe 21, the first reagent dispensing probe 23, the first stirrer 25, the second reagent dispensing probe 27, and the second stirrer 29 are the sample ejection position Pa and the first reagent ejection position. The operation of discharging and stirring the sample and reagent to the reaction container 19 for the inspection item B analysis that stops at the fifth time at Pb, the first stirring position Pc, the second reagent discharging position Pd, and the second stirring position Pe is stopped. Further, the cleaning nozzle 40 cleans the reaction container 19 for analysis of the inspection item B, which stops at the cleaning position W for the fifth time.

  The measurement unit 31 measures the liquid mixture in the reaction container 19 that has passed through the measurement position Pm from the first stop at the first stirring position Pc to the fifth stop at the second reagent discharge position Pd. Absorbance data is generated by measuring every Pm passage. Then, the calculation unit 61 generates analysis data for the inspection item B based on the absorbance data generated by the measurement unit 31. The output unit 70 outputs the analysis data of the inspection item B when the reaction container 19 for analysis of the inspection item B stops at the second reagent discharge position Pd in the fifth round.

  FIG. 9 is a diagram showing an example of an analysis parameter setting screen for the inspection item C displayed on the display unit 72. The analysis parameter setting screen 75 includes the same fields as the analysis parameter setting screen 73 of FIG.

  In the “item” column, “C” which is the item name of the inspection item C is displayed. In the “round” column, “5” indicating that the number of rounds is 5 is displayed. In the “reaction time” column, “T9” indicating that the reaction time of the mixed solution for analyzing the inspection item C is time T9 is displayed.

  In the “sample / diluent” column of the “sample parameter” column, “Sa” indicating that the test sample for analysis of the test item C is discharged into the reaction container 19 for analysis of the test item C is displayed. Has been. Further, “Sr” and “Sd” are displayed in the reaction container 19 to indicate that a test sample for retesting of the inspection item C and a diluted solution are to be discharged.

  In the “discharge amount (μL)” column, “2” indicating that 2 μL of the test sample for analysis of the test item C is discharged into the reaction container 19 for analysis of the test item C is displayed. Further, “10” indicating that 10 μL of the test sample for retesting of the inspection item B is discharged into the reaction container 19 is displayed, and “100” indicating that 100 μL of the diluted solution is discharged is displayed.

  In the “discharge round” column, “1” indicating that the test sample for analysis of the test item A is discharged into the reaction container 19 for analysis of the test item C that stops at the sample discharge position Pa in the first round is displayed. Has been. Further, the timing for discharging the test sample for retesting of the test item A and the diluent is not set in the reaction container 19 and is blank.

  As described above, when 5 is set as the number of rounds, the sample can be discharged into the reaction container 19 for analysis of the inspection item C at the sample discharge position Pa at the timing of 5 times, and one of the 5 times is set. It is possible to perform setting so that the test sample is discharged in the first round that is the timing of the second time.

  In the “reagent” column of the “reagent parameter” column, “R1” indicating that the reagent of the test item C is composed of the first to third reagents of the three reagent system and is the first reagent to be dispensed first. “R2” indicating that the second reagent is dispensed after the first reagent is dispensed, and “R3” indicating that the second reagent is dispensed after the second reagent is dispensed. It is displayed.

  In the “reagent name” column, “C1” indicating that the reagent name of the first reagent used for the analysis of the test item C is the reagent C1 is displayed, and the reagent name of the second reagent is the reagent C2. “C2” is displayed, and “C3” is displayed indicating that the reagent name of the third reagent is the reagent C3.

  In “reagent amount (μL)”, “100” indicating that the reagent amount of the first reagent discharged to the reaction container 19 for analysis of the test item C is 100 μL is displayed. Further, “60” indicating that the amount of the second reagent discharged to the reaction container 19 for analysis of the test item C is 60 μL is displayed. Further, “20” indicating that the reagent amount of the third reagent discharged to the reaction container 19 for analysis of the test item C is 20 μL is displayed.

  In the “discharge position” and “discharge round” columns, “Pb” indicating that the first reagent is discharged into the reaction container 19 for analysis of the test item C that stops at the first reagent discharge position Pb in the first round. And “1” are displayed. In addition, “Pb” and “2” indicating that the second reagent is discharged into the reaction container 19 for analysis of the inspection item B that stops at the first reagent discharge position Pb in the second round are displayed. In addition, “Pd” and “4” indicating that the third reagent is discharged into the reaction container 19 for analysis of the inspection item C that stops at the second reagent discharge position Pd in the fourth round are displayed.

  As described above, when 5 is set as the number of rounds, the reagent can be discharged into the reaction container 19 for analysis of the test item C at the first reagent discharge position Pb at the timing of 5 times. The first reagent can be discharged in the first round, which is the first timing, and the second reagent can be discharged in the second round, which is the second timing. In addition, the reagent can be discharged into the reaction container 19 for analysis of the inspection item C at the second reagent discharge position Pd at the timing of five times, and the third time is set at the fourth round which is the fourth timing among the five times. Settings for discharging the reagent can be made.

  In the column of “stirring position” and “stirring round” in the column of “stirring parameter”, stir the mixed solution in the reaction container 19 for the inspection item C analysis that stops at the first stirring position Pc in the first round. “Pc” and “1” are displayed. In addition, “Pc” and “2” indicating that the liquid mixture in the reaction container 19 for the inspection item C analysis that stops at the first stirring position Pc is stirred in the second round are displayed. In addition, “Pc” and “3” indicating that the liquid mixture in the reaction container 19 for the inspection item C analysis that stops at the first stirring position Pc is stirred in the third round are displayed. In addition, “Pe” and “4” indicating that the mixed liquid in the reaction container 19 for the inspection item B analysis that stops at the second stirring position Pc is stirred in the fourth round are displayed.

  As described above, when 5 is set as the number of rounds, the liquid mixture in the reaction container 19 for analysis of the inspection item C can be stirred at the first stirring position Pc at the timing of 5 times. It is possible to make a setting for stirring the mixed solution in the first to third rounds, which are the first to third timings. In addition, the liquid mixture in the reaction container 19 for analysis of the inspection item C can be stirred at the second stirring position Pe at the timing of five times, and stirred at the fourth round, which is the fourth timing among the five times. Can be set.

  The timing chart displayed in the “Reaction sequence” column is different from the timing chart displayed in the “Reaction sequence” column of the analysis parameter setting screen 74 shown in FIG. 8 in that the first agitation position in the third round. The point is that an arrow pointing downward indicates that the mixed liquid in the reaction vessel 19 stopped at the third time with Pc is stirred.

  The mechanism control unit 52 controls the moving mechanism unit 51 based on the analysis parameter displayed on the analysis parameter setting screen 75 to operate each analysis unit of the analysis unit 10.

  In the first round, the sample dispensing probe 21 is cleaned by the fifth cleaning nozzle 45 at the fifth cleaning position W5 and then stopped for the first time at the sample discharge position Pa. 2 μL of the test sample for analysis is discharged into the inside. Further, the first reagent dispensing probe 23 discharges 100 μL of the first reagent into the reaction container 19 for analysis of the test item C that stops for the first time at the first reagent discharge position Pb. Moreover, the 1st stirring element 25 stirs the liquid mixture in the reaction container 19 for the test item C analysis stopped at the 1st time at the 1st stirring position Pc. The second reagent dispensing probe 27, the first stirrer 25, and the washing nozzle 40 are used for the reaction for analysis of the test item C that stops at the first reagent discharge position Pb, the first stirring position Pc, and the washing position W for the first time. The operation of discharging, stirring and washing the reagent to the container 19 is stopped.

  In this way, the first reaction of the mixed liquid of the test sample and the first reagent is continued by stopping the cleaning of the reaction container 19 for analysis of the inspection item C that stops at the cleaning position W in the first round. be able to.

  In the second round, the sample dispensing probe 21 stops the discharge of the test sample into the reaction container 19 for the inspection item C analysis that stops at the second time at the sample discharge position Pa. Further, the first reagent dispensing probe 23 discharges 60 μL of the second reagent into the reaction container 19 for analysis of the test item C that stops for the second time at the first reagent discharge position Pb. Moreover, the 1st stirring element 25 stirs the liquid mixture in the reaction container 19 for the test | inspection item C analysis stopped for the 2nd time in the 1st stirring position Pc. The second reagent dispensing probe 27, the second stirrer 29, and the washing nozzle 40 are used for the reaction for analysis of the test item C that stops at the second reagent discharge position Pd, the second stirring position Pe, and the washing position W for the second time. The operation of discharging, stirring and washing the reagent to the container 19 is stopped.

  In this way, by stopping the cleaning of the reaction container 19 for analysis of the test item C that stops at the cleaning position W in the second round, the second reaction of the mixed liquid of the test sample and the first and second reagents is stopped. Can be continued.

  In the third round, the sample dispensing probe 21 and the first reagent dispensing probe 23 are a sample and a reagent for the reaction container 19 for the test item C analysis that stops at the third time at the sample ejection position Pa and the first reagent ejection position Pb. Stop the discharge operation. Moreover, the 1st stirring element 25 stirs the liquid mixture in the reaction container 19 for the test item C analysis stopped at the 3rd time in the 1st stirring position Pc. The second reagent dispensing probe 27, the second stirrer 29, and the washing nozzle 40 are used for the reaction for analysis of the test item C that stops at the second reagent discharge position Pd, the second stirring position Pe, and the washing position W for the third time. The operation of discharging, stirring and washing the reagent to the container 19 is stopped.

  In addition, an arrow indicating that the liquid mixture in the reaction container 19 for the inspection item C analysis that stops for the fourth time at the first stirring position Pc is set in the “Reaction sequence” column of the analysis parameter setting screen 75 is set. Thus, the mixed liquid can be stirred when the reaction container 19 for analysis of the inspection item C stops for the fourth time at the first stirring position Pc.

  Thus, even when the reagent is not discharged at the first reagent discharge position Pc in the third round, the liquid mixture in the reaction container 19 for analysis of the inspection item C is stirred when stopped at the first stirring position Pc. In addition, stirring is not performed for a long time, mixing becomes insufficient, and deterioration of analysis data of the inspection item C that adversely affects the reaction can be prevented.

  In the fourth round, the sample dispensing probe 21, the first reagent dispensing probe 23, and the first stirring bar 25 are inspected for the fourth time at the sample ejection position Pa, the first reagent ejection position Pb, and the first stirring position Pc. The operation of discharging and stirring the sample and reagent to the reaction container 19 for item C analysis is stopped. Further, the second reagent dispensing probe 27 discharges 20 μL of the third reagent into the reaction container 19 for analysis of the test item C that stops at the fourth time at the second reagent discharge position Pd. Moreover, the 2nd stirring element 29 stirs the liquid mixture in the reaction container 19 for the test item C analysis stopped at the 4th time in the 2nd stirring position Pe. Further, the cleaning nozzle 40 stops cleaning the reaction container 19 for analysis of the inspection item C, which stops at the cleaning position W for the fourth time.

  In this way, the third reaction of the mixed liquid of the test sample and the first to third reagents is stopped by stopping the cleaning of the reaction container 19 for analysis of the inspection item C that stops at the cleaning position W in the fourth round. Can be continued.

  In the fifth round, the sample dispensing probe 21, the first reagent dispensing probe 23, the first stirrer 25, the second reagent dispensing probe 27, and the second stirrer 29 are the sample ejection position Pa and the first reagent ejection position. The operation of discharging and stirring the sample and reagent to the reaction container 19 for the inspection item C analysis that stops at the fifth time at Pb, the first stirring position Pc, the second reagent discharging position Pd, and the second stirring position Pe is stopped. The cleaning nozzle 40 also cleans the reaction container 19 for analysis of the inspection item C that stops at the cleaning position W for the fifth time.

  The measurement unit 31 measures the liquid mixture in the reaction container 19 that has passed through the measurement position Pm from the first stop at the first stirring position Pc to the fifth stop at the second reagent discharge position Pd. Absorbance data is generated by measuring every Pm passage. Then, the calculation unit 61 generates analysis data for the inspection item C based on the absorbance data generated by the measurement unit 31. The output unit 70 outputs the analysis data of the inspection item C when the reaction container 19 for analysis of the inspection item C stops at the second reagent discharge position Pd in the fifth round.

  Thus, by setting the reaction time in the column of “Reaction time” on the analysis parameter setting screen 75, each test item C has a timing corresponding to the reaction time earlier than the end timing of the fifth round as the final round. Analysis data can be output.

  According to the embodiment described above, by setting 1 as the number of rounds by the input from the operation unit 80, the reaction container 19 for analysis of test items stopped at the cleaning position W for the first time after the cleaning is performed. Cleaning can be performed. In addition, by setting n as the number of rounds, the cleaning of the reaction container 19 for analysis of the inspection item can be stopped until the nth stop at the cleaning position W after the cleaning is performed. Then, the sample can be discharged into the reaction container 19 for analyzing the inspection item at the sample discharge position Pa at the timing of n times, and the sample can be discharged at the timing of at least one of the n times. In addition, the reagent can be discharged to the reaction container 19 for analyzing the test item at the first reagent discharge position Pb at the timing of n times, and the reagent can be discharged at least at the timing of the n times. . In addition, the liquid mixture in the reaction container 19 for analysis of the inspection item can be stirred at n times at the first stirring position Pc, and the liquid mixture is stirred at at least one of the n times. Can do. In addition, the reagent can be discharged into the reaction container 19 for analysis of the test item at the second reagent discharge position Pd at the timing of n times, and the reagent can be discharged at least at the timing of the n times. . Further, the liquid mixture in the reaction container 19 for analysis of the inspection item can be stirred at n times at the second stirring position Pe, and the liquid mixture is stirred at at least one of the n times. Can do.

  In the analysis of the inspection items B and C, the cleaning of the reaction container 19 for analysis of the inspection items B and C, which stops at the cleaning position W in the first round, is stopped, thereby mixing the test sample and the first reagent. The first reaction of the liquid can be continued. Further, by stopping the cleaning of the reaction container 19 for analysis of the inspection item A that stops at the cleaning position W in the first round, the second reaction of the mixed solution of the test sample and the first and second reagents is continued. Can be made. In addition, by stopping the cleaning of the reaction containers 19 for the inspection items B and C which are stopped at the cleaning position W in the second and third rounds, the first mixture liquid of the test sample and the first and second reagents is stopped. The reaction of 2 can be continued. Further, the third reaction of the mixed liquid of the test sample and the first to third reagents is stopped by stopping the cleaning of the reaction container 19 for analysis of the inspection items B and C that stops at the cleaning position W in the fourth round. Can be continued.

  This makes it possible to extend the time of the first reaction, the time of the second reaction, and the time of the third reaction without changing the time of one cycle time, thereby extending the time of one cycle time. A decrease in throughput can be reduced as compared to extending the reaction time.

  In addition, by setting n as the number of rounds, the first or second reagent dispensing probes 23 and 27 can be installed after the second round without providing a dedicated reagent dispensing probe for dispensing the third reagent. Used to dispense the second or third reagent. As a result, a larger number of first to third reagents than the number of the first and second reagent dispensing probes 23 and 27 are replaced with the first and second reagent dispensing probes 23 and 27, the first and second reagents. The reagent dispensing arms 24 and 28 and the moving mechanisms that drive the first and second reagent dispensing arms 24 and 28, respectively, can be used for dispensing, thereby preventing the automatic analyzer 100 from becoming complicated and large. be able to.

  Further, even when the reagent is not discharged at the first reagent discharge position Pc in the third round, the mixture in the reaction container 19 for analysis of the inspection item C is stirred when stopped at the first stirring position Pc. Stirring is not performed over time, mixing becomes insufficient, and deterioration of analysis data of inspection item C that adversely affects the reaction can be prevented.

  In addition, by setting the reaction time in the column of “Reaction time” of each analysis parameter setting screen 73, 74, 75, each test item A, B at a timing corresponding to the reaction time earlier than the end timing of the final round. , C analysis data can be output.

  As described above, the reaction time of a larger number of reagents than the number of the first and second reagent dispensing probes 23 and 27 can be extended.

  Although several embodiments of the present invention have been described, these embodiments are presented by way of example and are not intended to limit the scope of the invention. These novel embodiments can be implemented in various other forms, and various omissions, replacements, and changes can be made without departing from the scope of the invention. These embodiments and modifications thereof are included in the scope and gist of the invention, and are included in the invention described in the claims and the equivalents thereof.

Pa Sample discharge position Pb First reagent discharge position Pc First stirring position Pd Second reagent discharge position Pe Second stirring position W Washing position 19 Reaction vessel 21 Sample dispensing probe 23 First reagent dispensing probe 25 First stirring bar 27 Second reagent dispensing probe 29 Second stirrer 31 Measuring unit 40 Washing nozzle 50 Analysis control unit 51 Movement mechanism unit 52 Mechanism control unit 100 Automatic analyzer

Claims (5)

In an automatic analyzer that measures a mixture of a sample and a reagent at a predetermined measurement position,
Cleaning position and moved for each cycle time, the sample discharging position, a reagent discharge position, and a stirring position stops at including the stop position, it stopped before the cycle time of the predetermined number of times after the cycle time of a predetermined number of times It moves at a stop position until a reaction vessel to stop the,
Among the reaction vessels stopped at the washing position, a washing nozzle for washing the reaction vessel containing the mixed liquid that has finished the measurement ,
Of the reaction vessel was stopped at the sample discharge position, the sample dispensing probe for discharging the sample cleaning performed in the reaction vessel is housed an empty reaction vessel or the reagent according to the cleaning nozzle,
Of the reaction vessel was stopped at the reagent discharging position, a reagent which discharges the reagent into a reaction vessel a sample cleaning by the cleaning nozzle is ejected by performed by an empty reaction vessel or the sample dispensing probe is accommodated A dispensing probe;
Of the reaction vessel was stopped by the stirring position, and stirrer for stirring the mixed solution in the reaction vessel in which the mixed solution is accommodated,
A measurement unit for measuring the mixed liquid in the reaction vessel that passes through the measurement position after being stirred by the stirring bar;
An analysis control unit that drives and controls the reaction container, the washing nozzle, the sample dispensing probe, the reagent dispensing probe, and the stirring bar;
A calculation unit that generates calibration data based on standard data generated by measurement of the measurement unit when the sample is a standard sample;
The analysis control section, the wash after washing by that earlier cleaning nozzles for a particular reaction vessel is made, the destination of cleaning performed by either et previous SL cleaning position with n (2 or more integer ) times was sealed stop at stop until the n times the discharge of the reagent to the reaction vessel, with the reagent discharge position during a period from the destination of the cleaning is performed until stopped n times with the washing position It was done when stopping at least one of stopping,
The measuring unit is configured to perform n cleaning at the cleaning position after the previous cleaning is performed on the reaction container in which the subsequent cleaning is stopped until the subsequent cleaning is stopped n times after the previous cleaning is performed. Until it is stopped once, a plurality of standard data is generated by measuring the mixed solution of the standard sample and the reagent in the reaction container every time the measurement position passes,
The automatic calculation apparatus, wherein the calculation unit generates at least one calibration data based on at least one standard data among a plurality of standard data generated by measurement of the measurement unit. The analysis control unit stops the subsequent cleaning with respect to the specific reaction container that has been previously cleaned by the cleaning nozzle until the reaction is stopped n times at the cleaning position after the previous cleaning is performed. stirring of the mixture of the sample and the reagent with respect to the container, the destination of at least one of stopping the n washes from taking place by the stirring positioned until the stop n times with the washing position The automatic analyzer according to claim 1, wherein the automatic analyzer is performed when stopped . The analysis control unit stops the subsequent cleaning with respect to the specific reaction container that has been previously cleaned by the cleaning nozzle until the reaction is stopped n times at the cleaning position after the previous cleaning is performed. performed when the discharge of the sample with respect to the container, and at least one stop of stopping the n times with the sample discharge position during a period from the destination of the cleaning is carried out until the stop n times with the washing position The automatic analyzer according to claim 1, wherein: When the sample is a test sample, the measurement unit performs the previous cleaning on the reaction vessel in which the subsequent cleaning is stopped until the cleaning is stopped n times after the previous cleaning is performed. between performed with up to stop n times with the washing position, generating a plurality of test data mixture by measuring of the test sample and the reagent in the reaction vessel for each passage of the measuring positions And
The arithmetic unit, and generating analysis data based on at least one of the test data and the at least one calibration data of the plurality of test data generated by the measurement before Symbol measuring unit The automatic analyzer according to claim 1 . The cleaning nozzle is pre-Symbol the first cleaning nozzle and said first stop the mixture was completed the measurement to wash the reaction vessel is housed within the reaction vessel was stopped at the first cleaning position of the cleaning position A second cleaning nozzle for cleaning the reaction vessel cleaned by the first cleaning nozzle stopped at the second cleaning position after stopping at the position;
Wherein the first and the second cleaning nozzle is moved independently, stopped in finished the reaction vessel mixture is accommodated and the second cleaning position the measurement stopped at the first cleaning position The automatic analyzer according to any one of claims 1 to 4 , wherein the reaction vessel cleaned by the first cleaning nozzle enters and stops at a position where cleaning can be performed .

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