JPWO2012002541A1 - 標的分子の検出法 - Google Patents

標的分子の検出法 Download PDF

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Publication number
JPWO2012002541A1
JPWO2012002541A1 JP2011548712A JP2011548712A JPWO2012002541A1 JP WO2012002541 A1 JPWO2012002541 A1 JP WO2012002541A1 JP 2011548712 A JP2011548712 A JP 2011548712A JP 2011548712 A JP2011548712 A JP 2011548712A JP WO2012002541 A1 JPWO2012002541 A1 JP WO2012002541A1
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target molecule
nucleic acid
oligonucleotide
sequence
population
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JP2011548712A
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English (en)
Japanese (ja)
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真 宮岸
真 宮岸
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National Institute of Advanced Industrial Science and Technology AIST
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National Institute of Advanced Industrial Science and Technology AIST
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2011548712A 2010-07-01 2011-07-01 標的分子の検出法 Pending JPWO2012002541A1 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2010151242 2010-07-01
JP2010151242 2010-07-01
PCT/JP2011/065205 WO2012002541A1 (ja) 2010-07-01 2011-07-01 標的分子の検出法

Related Child Applications (1)

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JP2012110792A Division JP2012198225A (ja) 2010-07-01 2012-05-14 標的分子の検出法

Publications (1)

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JPWO2012002541A1 true JPWO2012002541A1 (ja) 2013-08-29

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Family Applications (2)

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JP2011548712A Pending JPWO2012002541A1 (ja) 2010-07-01 2011-07-01 標的分子の検出法
JP2012110792A Pending JP2012198225A (ja) 2010-07-01 2012-05-14 標的分子の検出法

Family Applications After (1)

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JP2012110792A Pending JP2012198225A (ja) 2010-07-01 2012-05-14 標的分子の検出法

Country Status (4)

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US (1) US20130116129A1 (https=)
EP (1) EP2589657A4 (https=)
JP (2) JPWO2012002541A1 (https=)
WO (1) WO2012002541A1 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012001820A1 (en) * 2010-07-02 2012-01-05 National University Corporation Tokyo University Of Agriculture And Technology Psa binding aptamer and method for diagnosis of prostate cancer
WO2013141291A1 (ja) * 2012-03-23 2013-09-26 Ｎｅｃソフト株式会社 ストレプトアビジンの分析用デバイスおよび分析方法
JP5999786B2 (ja) 2012-03-23 2016-09-28 Ｎｅｃソリューションイノベータ株式会社 Ａｔｐまたはａｍｐの分析用デバイスおよび分析方法
JP5731016B2 (ja) * 2012-04-27 2015-06-10 キリン株式会社 リガンドを高感度に検出する核酸分子並びに該核酸分子のスクリーニング方法および該核酸分子の感度の最適化方法
EP4170031A1 (en) 2012-10-23 2023-04-26 Caris Science, Inc. Aptamers and uses thereof
US10942184B2 (en) 2012-10-23 2021-03-09 Caris Science, Inc. Aptamers and uses thereof
AU2013361323B2 (en) 2012-12-19 2018-09-06 Caris Science, Inc. Compositions and methods for aptamer screening
US9828599B2 (en) 2013-09-18 2017-11-28 General Electric Company Methods of selecting binding-elements and uses thereof
US10359419B2 (en) 2013-10-02 2019-07-23 General Electric Company Methods for detection of target using affinity binding
JP6128621B2 (ja) * 2013-12-04 2017-05-17 国立研究開発法人産業技術総合研究所 マイクロベシクルに対する核酸アプタマー
CN105483267B (zh) * 2016-01-15 2018-12-04 古博 血浆游离DNA双分子标记、标记和检测血浆cfDNA的方法及其用途
JP7146839B2 (ja) * 2019-11-15 2022-10-04 花王株式会社 シーケンシング用ライブラリの調製方法
US20220396825A1 (en) 2019-11-15 2022-12-15 Kao Corporation Method for preparing sequencing library
CN113699215B (zh) * 2021-08-13 2024-06-14 东南大学 一种核酸适体筛选方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997038134A1 (en) * 1996-04-05 1997-10-16 Nexstar Pharmaceuticals, Inc. Method for detecting a target compound using a nucleic acid ligand
JP2008102675A (ja) * 2006-10-18 2008-05-01 Nec Soft Ltd 塩基配列の同定方法及び核酸分子の二次構造取得方法、並びにこれらを実行する装置及びプログラム
WO2009053691A1 (en) * 2007-10-22 2009-04-30 Pronota Nv Method of selecting aptamers
JP2009189310A (ja) * 2008-02-15 2009-08-27 Olympus Corp 高親和性核酸フラグメント群の塩基配列決定方法および核酸アプタマーのスクリーニング方法
WO2009107803A1 (ja) * 2008-02-27 2009-09-03 ソニー株式会社 標的物質の定量方法

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US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
ES2259800T3 (es) 1990-06-11 2006-10-16 Gilead Sciences, Inc. Procedimientos de uso de ligandos de acido nucleico.
US20090053138A1 (en) * 2002-11-21 2009-02-26 Preiss Jeffrey R Stabilized Aptamers to Platelet Derived Growth Factor and their Use as Oncology Therapeutics
JP2008161165A (ja) 2006-12-28 2008-07-17 Dnaform:Kk 競合オリゴヌクレオチドを用いた遺伝子検出法
ES2448426T3 (es) * 2007-10-12 2014-03-13 Pronota Nv Uso de aptámeros en proteómica
EP2260103A4 (en) * 2008-03-12 2012-06-13 Univ Syracuse DIRECT SELECTION OF STRUCTURAL DEFINED APTAMERS
JP2010041951A (ja) 2008-08-11 2010-02-25 Olympus Corp 標的分子の検出方法
KR101769743B1 (ko) * 2008-08-15 2017-08-21 코넬 유니버시티 특이적 화학적 타겟과 결합하는 핵산의 신속한 확인을 위한 장치
JP5256016B2 (ja) 2008-12-25 2013-08-07 コスモ工機株式会社 管接続部材の脱着装置
JP5419145B2 (ja) * 2009-07-24 2014-02-19 Ｎｅｃソフト株式会社 アプタマー分類装置、アプタマー分類方法、プログラムおよび記録媒体

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997038134A1 (en) * 1996-04-05 1997-10-16 Nexstar Pharmaceuticals, Inc. Method for detecting a target compound using a nucleic acid ligand
JP2008102675A (ja) * 2006-10-18 2008-05-01 Nec Soft Ltd 塩基配列の同定方法及び核酸分子の二次構造取得方法、並びにこれらを実行する装置及びプログラム
WO2009053691A1 (en) * 2007-10-22 2009-04-30 Pronota Nv Method of selecting aptamers
JP2009189310A (ja) * 2008-02-15 2009-08-27 Olympus Corp 高親和性核酸フラグメント群の塩基配列決定方法および核酸アプタマーのスクリーニング方法
WO2009107803A1 (ja) * 2008-02-27 2009-09-03 ソニー株式会社 標的物質の定量方法

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
JPN6012011892; ＢＭＣ Ｂｉｏｉｎｆｏｒｍａｔｉｃｓ Ｖｏｌ．９, 2008, ｄｏｉ：１０．１１８６／１４７１-２１０５-９-１７６ *
JPN6012011894; Ｇｅｎｏｍｅ Ｒｅｓ． Ｖｏｌ．２０， Ｎｏ．６, 201006, ｐｐ．８６１-８７３ *
JPN6012011896; ＢＭＣ Ｂｉｏｉｎｆｏｒｍａｔｉｃｓ Ｖｏｌ．７， Ｓｕｐｐｌ．２, 2006, ｄｏｉ：１０．１１８６／１４７１-２１０５-７-Ｓ２-Ｓ６ *
JPN6012011898; Ｂｉｏｔｅｃｈｎｏｌ． Ｌｅｔｔ． Ｖｏｌ．３２， Ｎｏ．３, 201003, ｐｐ．３７９-３８５ *
JPN6012011901; Ｂｉｏｍｏｌ． Ｅｎｇ． Ｖｏｌ．２４， Ｎｏ．２, 2007, ｐｐ．１７９-１８９ *
JPN6012011907; Ｃｌｉｎ． Ｃｈｅｍ． Ｖｏｌ．５５， Ｎｏ．９, 200909, ｐｐ．１６８６-１６９３ *

Also Published As

Publication number Publication date
EP2589657A4 (en) 2014-04-23
EP2589657A1 (en) 2013-05-08
JP2012198225A (ja) 2012-10-18
US20130116129A1 (en) 2013-05-09
WO2012002541A1 (ja) 2012-01-05
EP2589657A9 (en) 2013-10-16

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