JPWO2011158845A1 - 誘導多能性幹細胞の製造方法、及びその製造方法により得られる誘導多能性幹細胞 - Google Patents
誘導多能性幹細胞の製造方法、及びその製造方法により得られる誘導多能性幹細胞 Download PDFInfo
- Publication number
- JPWO2011158845A1 JPWO2011158845A1 JP2012520464A JP2012520464A JPWO2011158845A1 JP WO2011158845 A1 JPWO2011158845 A1 JP WO2011158845A1 JP 2012520464 A JP2012520464 A JP 2012520464A JP 2012520464 A JP2012520464 A JP 2012520464A JP WO2011158845 A1 JPWO2011158845 A1 JP WO2011158845A1
- Authority
- JP
- Japan
- Prior art keywords
- cells
- pluripotent stem
- induced pluripotent
- pulse power
- producing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000004263 induced pluripotent stem cell Anatomy 0.000 title claims abstract description 87
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 54
- 210000004027 cell Anatomy 0.000 claims abstract description 131
- 101150012532 NANOG gene Proteins 0.000 claims abstract description 63
- 235000013601 eggs Nutrition 0.000 claims abstract description 61
- 210000001082 somatic cell Anatomy 0.000 claims abstract description 52
- 238000000034 method Methods 0.000 claims description 24
- 230000014509 gene expression Effects 0.000 claims description 22
- 210000004602 germ cell Anatomy 0.000 claims description 14
- 241000251468 Actinopterygii Species 0.000 claims description 10
- 230000005684 electric field Effects 0.000 claims description 7
- 241000252233 Cyprinus carpio Species 0.000 claims description 4
- 210000000130 stem cell Anatomy 0.000 claims description 3
- 230000006698 induction Effects 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 description 27
- 206010036790 Productive cough Diseases 0.000 description 26
- 210000003802 sputum Anatomy 0.000 description 26
- 208000024794 sputum Diseases 0.000 description 26
- 241000276569 Oryzias latipes Species 0.000 description 19
- 239000003550 marker Substances 0.000 description 10
- 238000011282 treatment Methods 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 235000019688 fish Nutrition 0.000 description 8
- 238000011529 RT qPCR Methods 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 241000894007 species Species 0.000 description 7
- 101710126211 POU domain, class 5, transcription factor 1 Proteins 0.000 description 6
- 238000012258 culturing Methods 0.000 description 6
- 238000011161 development Methods 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- 230000004069 differentiation Effects 0.000 description 6
- 238000010195 expression analysis Methods 0.000 description 6
- 230000008672 reprogramming Effects 0.000 description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 5
- 102100035423 POU domain, class 5, transcription factor 1 Human genes 0.000 description 5
- 239000002771 cell marker Substances 0.000 description 5
- 239000012091 fetal bovine serum Substances 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 238000002054 transplantation Methods 0.000 description 5
- 241000251730 Chondrichthyes Species 0.000 description 4
- 101150039798 MYC gene Proteins 0.000 description 4
- 238000009004 PCR Kit Methods 0.000 description 4
- CGNLCCVKSWNSDG-UHFFFAOYSA-N SYBR Green I Chemical compound CN(C)CCCN(CCC)C1=CC(C=C2N(C3=CC=CC=C3S2)C)=C2C=CC=CC2=[N+]1C1=CC=CC=C1 CGNLCCVKSWNSDG-UHFFFAOYSA-N 0.000 description 4
- 101150037203 Sox2 gene Proteins 0.000 description 4
- 210000004413 cardiac myocyte Anatomy 0.000 description 4
- 239000002299 complementary DNA Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 210000003494 hepatocyte Anatomy 0.000 description 4
- 238000003753 real-time PCR Methods 0.000 description 4
- 230000009261 transgenic effect Effects 0.000 description 4
- 210000002237 B-cell of pancreatic islet Anatomy 0.000 description 3
- 102000029816 Collagenase Human genes 0.000 description 3
- 108060005980 Collagenase Proteins 0.000 description 3
- 101100351026 Drosophila melanogaster ey gene Proteins 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 102000007354 PAX6 Transcription Factor Human genes 0.000 description 3
- 101150081664 PAX6 gene Proteins 0.000 description 3
- 208000018737 Parkinson disease Diseases 0.000 description 3
- 102000004142 Trypsin Human genes 0.000 description 3
- 108090000631 Trypsin Proteins 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 210000002950 fibroblast Anatomy 0.000 description 3
- 230000001172 regenerating effect Effects 0.000 description 3
- 239000012588 trypsin Substances 0.000 description 3
- 208000024827 Alzheimer disease Diseases 0.000 description 2
- 101100179813 Drosophila melanogaster Actbeta gene Proteins 0.000 description 2
- 206010019280 Heart failures Diseases 0.000 description 2
- 102000003745 Hepatocyte Growth Factor Human genes 0.000 description 2
- 108090000100 Hepatocyte Growth Factor Proteins 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 101710135898 Myc proto-oncogene protein Proteins 0.000 description 2
- 101710150448 Transcriptional regulator Myc Proteins 0.000 description 2
- 206010067584 Type 1 diabetes mellitus Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 210000001015 abdomen Anatomy 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 206010008118 cerebral infarction Diseases 0.000 description 2
- 229960002424 collagenase Drugs 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 210000005064 dopaminergic neuron Anatomy 0.000 description 2
- 210000002242 embryoid body Anatomy 0.000 description 2
- 210000001671 embryonic stem cell Anatomy 0.000 description 2
- 210000002257 embryonic structure Anatomy 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 108010021843 fluorescent protein 583 Proteins 0.000 description 2
- 210000003897 hepatic stem cell Anatomy 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 230000009027 insemination Effects 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 108700024542 myc Genes Proteins 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 210000000006 pectoral fin Anatomy 0.000 description 2
- 210000001778 pluripotent stem cell Anatomy 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 208000020431 spinal cord injury Diseases 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 239000013598 vector Substances 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 241000938605 Crocodylia Species 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 206010019663 Hepatic failure Diseases 0.000 description 1
- 102100038895 Myc proto-oncogene protein Human genes 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 208000016222 Pancreatic disease Diseases 0.000 description 1
- 108010023082 activin A Proteins 0.000 description 1
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 208000015114 central nervous system disease Diseases 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 230000007882 cirrhosis Effects 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 239000003862 glucocorticoid Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- -1 klf4 Proteins 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 208000007903 liver failure Diseases 0.000 description 1
- 231100000835 liver failure Toxicity 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000000472 morula Anatomy 0.000 description 1
- 201000006417 multiple sclerosis Diseases 0.000 description 1
- 208000031225 myocardial ischemia Diseases 0.000 description 1
- 210000001577 neostriatum Anatomy 0.000 description 1
- 210000001178 neural stem cell Anatomy 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 102000045246 noggin Human genes 0.000 description 1
- 108700007229 noggin Proteins 0.000 description 1
- 230000011599 ovarian follicle development Effects 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 208000024691 pancreas disease Diseases 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 108010054624 red fluorescent protein Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229930002330 retinoic acid Natural products 0.000 description 1
- 230000001177 retroviral effect Effects 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000009168 stem cell therapy Methods 0.000 description 1
- 238000009580 stem-cell therapy Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 210000002536 stromal cell Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 229960001727 tretinoin Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2529/00—Culture process characterised by the use of electromagnetic stimulation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Transplantation (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2010135921 | 2010-06-15 | ||
| JP2010135921 | 2010-06-15 | ||
| PCT/JP2011/063632 WO2011158845A1 (fr) | 2010-06-15 | 2011-06-15 | Procédé de production de cellules souches pluripotentes induites et cellules souches pluripotentes induites produites par le procédé |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPWO2011158845A1 true JPWO2011158845A1 (ja) | 2013-08-19 |
Family
ID=45348242
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012520464A Pending JPWO2011158845A1 (ja) | 2010-06-15 | 2011-06-15 | 誘導多能性幹細胞の製造方法、及びその製造方法により得られる誘導多能性幹細胞 |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JPWO2011158845A1 (fr) |
| WO (1) | WO2011158845A1 (fr) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ITGE20120073A1 (it) | 2012-07-23 | 2014-01-24 | Carlo Tremolada | Metodo e dispositivo per la preparazione di cellule staminali non embrionali |
| JP5774657B2 (ja) * | 2013-10-04 | 2015-09-09 | 国立大学法人京都大学 | エレクトロポレーションを利用した哺乳類の遺伝子改変方法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4214742B2 (ja) * | 2002-08-29 | 2009-01-28 | 独立行政法人科学技術振興機構 | 電気刺激装置及びその電気刺激装置を用いた生体細胞若しくは生体組織の成長促進又は抑制方法 |
| JP2004105148A (ja) * | 2002-09-20 | 2004-04-08 | National Cardiovascular Center | 細胞分化誘導法 |
| JP4389037B2 (ja) * | 2003-06-03 | 2009-12-24 | 独立行政法人理化学研究所 | Ｅｓ細胞の電気パルス処理によって得られた神経細胞 |
-
2011
- 2011-06-15 JP JP2012520464A patent/JPWO2011158845A1/ja active Pending
- 2011-06-15 WO PCT/JP2011/063632 patent/WO2011158845A1/fr active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2011158845A1 (fr) | 2011-12-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6080871B2 (ja) | 神経幹細胞を調製するための培養培地およびその使用 | |
| US20060247195A1 (en) | Method of altering cell properties by administering rna | |
| JP6868565B2 (ja) | 不死化幹細胞、及びその作製方法 | |
| Tanabe et al. | Induction of pluripotency by defined factors | |
| CN1860222A (zh) | 用于临床和商业用途的干细胞 | |
| CN105154407B (zh) | 一种皮肤修复功能提高的人脂肪干细胞的制备方法及其应用 | |
| CN102796696B (zh) | 一种从人皮肤细胞直接诱导的神经元细胞及其制备方法 | |
| CN107893049A (zh) | 产生诱导多能干细胞和分化细胞的方法 | |
| WO2011158845A1 (fr) | Procédé de production de cellules souches pluripotentes induites et cellules souches pluripotentes induites produites par le procédé | |
| JP6040494B2 (ja) | 発酵能を有する細菌を用いた多能性細胞の製造方法 | |
| KR101548534B1 (ko) | 전자기장을 이용한 성체세포를 유도만능 줄기세포로 역분화시키는 방법 | |
| JP6698626B2 (ja) | 哺乳動物多能性幹細胞、それらの調製のための方法、およびその使用 | |
| JP2011004674A (ja) | 誘導多能性幹細胞（ｉＰＳ細胞）の製造方法 | |
| JP6785516B2 (ja) | ヒト臍帯由来間葉系幹細胞から骨芽細胞の製造を目的としたアクチン重合阻害剤による分化誘導技術 | |
| KR101671882B1 (ko) | 지방-유래 중간엽 줄기세포로부터 유도만능 줄기세포를 제조하여 신경세포로 분화 시키는 방법 | |
| Li et al. | Using microRNAs to enhance the generation of induced pluripotent stem cells | |
| CN105073978B (zh) | 利用植物干细胞或植物去分化干细胞的提取物诱导定制亚全能干细胞的方法以及利用该方法的方式制得的亚全能干细胞 | |
| Gardlik | Inducing pluripotency using in vivo gene therapy | |
| KR20130028294A (ko) | 개과 동물의 와튼 젤리-유래 중간엽 줄기세포의 분리 및 특성화 | |
| WO2021081721A1 (fr) | Nouvelles cellules souches pluripotentes induites (ipsc) et leurs applications | |
| US20220243175A1 (en) | Method for isolating and culturing neural stem cells with high efficiency | |
| KR101744053B1 (ko) | 인장 자극을 이용한 유도만능 줄기세포의 생성 방법 | |
| TWI769410B (zh) | 新穎誘導性多能幹細胞(ipscs)及其應用 | |
| US20230174949A1 (en) | Engineered vascularized organoids | |
| Huang et al. | Induced pluripotent stem cell technologies for tissue engineering |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20140611 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20140611 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20150915 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20160126 |