JPWO2006011296A1 - 軟骨細胞の三次元培養方法 - Google Patents
軟骨細胞の三次元培養方法 Download PDFInfo
- Publication number
- JPWO2006011296A1 JPWO2006011296A1 JP2006528429A JP2006528429A JPWO2006011296A1 JP WO2006011296 A1 JPWO2006011296 A1 JP WO2006011296A1 JP 2006528429 A JP2006528429 A JP 2006528429A JP 2006528429 A JP2006528429 A JP 2006528429A JP WO2006011296 A1 JPWO2006011296 A1 JP WO2006011296A1
- Authority
- JP
- Japan
- Prior art keywords
- chondrocytes
- collagen
- gel
- group
- proteoglycan
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3817—Cartilage-forming cells, e.g. pre-chondrocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
- A61L27/3843—Connective tissue
- A61L27/3852—Cartilage, e.g. meniscus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0655—Chondrocytes; Cartilage
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Rheumatology (AREA)
- Dermatology (AREA)
- Botany (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Vascular Medicine (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Developmental Biology & Embryology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Physical Education & Sports Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Immunology (AREA)
- Materials For Medical Uses (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Description
項1.軟骨細胞をコラーゲン及びプロテオグリカンを含むゲル状構造物中に包埋した状態で培養することを特徴とする軟骨細胞の三次元培養方法。
項2.コラーゲンとプロテオグリカンの比率が1:0.3〜1.1(重量比)である項1に記載の方法。
項3.コラーゲン及びプロテオグリカンからなるゲル状構造物中で軟骨細胞を増殖させることを特徴とする軟骨細胞の生産方法。
項4.コラーゲン及びプロテオグリカンからなるゲル状構造物中に軟骨細胞を包埋してなる軟骨組織を再生可能なゲル状構造物。
項5.軟骨組織損傷部への移植用材である項4のゲル状構造物。
項6.少なくとも軟骨細胞、コラーゲン及びプロテオグリカンを含む軟骨組織研究用キット。
実施例1
軟骨細胞の培養
ウサギ膝関節軟骨から分離された軟骨細胞(株式会社ホクドーより購入)を用いた。軟骨細胞は、1世代継代されたものを実験に用いた。また、培養用メデイウムは、10%FBSおよび100μMアスコルビン酸を含むRPMI1670培地(株式会社ホクドー)を用いた。3%I型アテロコラーゲン:(プロテオグリカン+培養用メデイウム)=65μl:60μlとなるように混合し、これに軟骨細胞 約1X105個を加えた。(アテロコラーゲン:プロテオグリカンの重量での比率 A群(1:0)、B群(1:0.05)、C群(1:0.51)、D群(1:2.56))次いで十分に攪拌後、35 mmデイッシュに滴下し、37℃で10分間インキュベーションしゲル化させた。ゲル化が良好であることを確認後、培養用メデイウムを2.5 ml加えた。 5%CO2で37℃の下培養を行なった。また、2回/週で培地交換を行ない、21日間培養を行なった。
組織学的及び免疫組織学的評価
培養21日目に、標本を10%ホルマリンで固定し、ヘマトキシリン・エオジン染色とアルシアンブルー染色を行なった。また、硝子軟骨の重要基質であるII型コラーゲンの存在を確認するために、抗ヒトコラーゲンII型抗体(第一ファインケミカル株式会社)で免疫染色を行なった。なお、図2a, 3a, 4aは、A群を図2b, 3b, 4bは、B群を図2c, 3c, 4cは、C群を示す。
Claims (6)
- 軟骨細胞をコラーゲン及びプロテオグリカンを含むゲル状構造物中に包埋した状態で培養することを特徴とする軟骨細胞の三次元培養方法。
- コラーゲンとプロテオグリカンの比率が1:0.3〜1.1(重量比)である請求項1に記載の方法。
- コラーゲン及びプロテオグリカンからなるゲル状構造物中で軟骨細胞を増殖させることを特徴とする軟骨細胞の生産方法。
- コラーゲン及びプロテオグリカンからなるゲル状構造物中に軟骨細胞を包埋してなる軟骨組織を再生可能なゲル状構造物。
- 軟骨組織損傷部への移植用材である請求項4のゲル状構造物。
- 少なくとも軟骨細胞、コラーゲン及びプロテオグリカンを含む軟骨組織研究用キット。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2006528429A JP4936892B2 (ja) | 2004-07-30 | 2005-06-01 | 軟骨細胞の三次元培養方法 |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2004223616 | 2004-07-30 | ||
JP2004223616 | 2004-07-30 | ||
PCT/JP2005/010038 WO2006011296A1 (ja) | 2004-07-30 | 2005-06-01 | 軟骨細胞の三次元培養方法 |
JP2006528429A JP4936892B2 (ja) | 2004-07-30 | 2005-06-01 | 軟骨細胞の三次元培養方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2006011296A1 true JPWO2006011296A1 (ja) | 2008-05-01 |
JP4936892B2 JP4936892B2 (ja) | 2012-05-23 |
Family
ID=35786050
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2006528429A Expired - Fee Related JP4936892B2 (ja) | 2004-07-30 | 2005-06-01 | 軟骨細胞の三次元培養方法 |
Country Status (4)
Country | Link |
---|---|
US (2) | US7931894B2 (ja) |
JP (1) | JP4936892B2 (ja) |
CA (1) | CA2575516C (ja) |
WO (1) | WO2006011296A1 (ja) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007032404A1 (ja) * | 2005-09-16 | 2007-03-22 | St. Marianna University, School Of Medicine | 再生医療用バイオマテリアル |
JP6324733B2 (ja) * | 2014-01-17 | 2018-05-16 | 株式会社ファンケル | コラーゲンの三重螺旋構造安定化剤 |
CN108475034B (zh) * | 2015-12-25 | 2021-04-13 | 佳能株式会社 | 定影装置和成像装置 |
US20210230526A1 (en) | 2016-06-28 | 2021-07-29 | National Agriculture And Food Research Organization | Cell enclosure device and use for same |
EP3578638A4 (en) * | 2017-01-31 | 2020-10-14 | Toppan Printing Co., Ltd. | THREE-DIMENSIONAL TISSUE BODY, METHOD OF MANUFACTURING THEREOF AND MOLDING AGENT FOR THREE-DIMENSIONAL TISSUE BODY |
WO2019135494A1 (ko) | 2018-01-08 | 2019-07-11 | 주식회사 에스오에스랩 | 라이다 장치 |
US10591598B2 (en) | 2018-01-08 | 2020-03-17 | SOS Lab co., Ltd | Lidar device |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2001224678A (ja) * | 2000-02-17 | 2001-08-21 | Mitsuo Ochi | 軟骨移植用材料の製造方法 |
JP2001514551A (ja) * | 1997-03-07 | 2001-09-11 | アボネティクス リミティッド | 組織移植体 |
JP2002537896A (ja) * | 1999-03-01 | 2002-11-12 | ラッシュ−プレズビテリアン−セント ルークス メディカル センター | 可移植性軟骨組織のinvitroでの製造 |
JP2003111831A (ja) * | 2001-07-30 | 2003-04-15 | Olympus Optical Co Ltd | 骨軟骨移植材 |
JP2007503852A (ja) * | 2003-08-20 | 2007-03-01 | ヒストジェニックス コーポレイション | 関節軟骨、骨、骨軟骨の欠損及び傷害を治療するための非細胞基質インプラント及びその使用方法 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CZ289772B6 (cs) * | 1997-03-10 | 2002-04-17 | Revmatologický Ústav | Biologický materiál na bázi kolagenu |
US6442222B1 (en) * | 1998-12-24 | 2002-08-27 | At&T Wireless Services, Inc. | Method for error compensation in an OFDM system with diversity |
US20040151705A1 (en) * | 2002-03-22 | 2004-08-05 | Shuichi Mizuno | Neo-cartilage constructs and a method for preparation thereof |
CA2441994C (en) * | 2001-03-23 | 2012-08-14 | Laurence J. Berlowitz Tarrant | Composition and methods for the production of biological tissues and tissue constructs |
US6444222B1 (en) * | 2001-05-08 | 2002-09-03 | Verigen Transplantation Services International Ag | Reinforced matrices |
WO2003084385A2 (en) * | 2002-04-02 | 2003-10-16 | William Marsh Rice University | Redifferentiated cells for repairing cartilage defects |
-
2005
- 2005-06-01 JP JP2006528429A patent/JP4936892B2/ja not_active Expired - Fee Related
- 2005-06-01 WO PCT/JP2005/010038 patent/WO2006011296A1/ja active Application Filing
- 2005-06-01 CA CA2575516A patent/CA2575516C/en not_active Expired - Fee Related
- 2005-06-01 US US11/658,744 patent/US7931894B2/en not_active Expired - Fee Related
-
2011
- 2011-02-03 US US13/020,498 patent/US9238092B2/en not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2001514551A (ja) * | 1997-03-07 | 2001-09-11 | アボネティクス リミティッド | 組織移植体 |
JP2002537896A (ja) * | 1999-03-01 | 2002-11-12 | ラッシュ−プレズビテリアン−セント ルークス メディカル センター | 可移植性軟骨組織のinvitroでの製造 |
JP2001224678A (ja) * | 2000-02-17 | 2001-08-21 | Mitsuo Ochi | 軟骨移植用材料の製造方法 |
JP2003111831A (ja) * | 2001-07-30 | 2003-04-15 | Olympus Optical Co Ltd | 骨軟骨移植材 |
JP2007503852A (ja) * | 2003-08-20 | 2007-03-01 | ヒストジェニックス コーポレイション | 関節軟骨、骨、骨軟骨の欠損及び傷害を治療するための非細胞基質インプラント及びその使用方法 |
Also Published As
Publication number | Publication date |
---|---|
WO2006011296A1 (ja) | 2006-02-02 |
CA2575516C (en) | 2014-03-18 |
US20110150847A1 (en) | 2011-06-23 |
JP4936892B2 (ja) | 2012-05-23 |
US9238092B2 (en) | 2016-01-19 |
US7931894B2 (en) | 2011-04-26 |
US20090004151A1 (en) | 2009-01-01 |
CA2575516A1 (en) | 2006-02-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Toh et al. | Potential of human embryonic stem cells in cartilage tissue engineering and regenerative medicine | |
JP4936892B2 (ja) | 軟骨細胞の三次元培養方法 | |
Flanagan et al. | Living artificial heart valve alternatives: a review | |
Van Susante et al. | Resurfacing potential of heterologous chondrocytes suspended in fibrin glue in large full-thickness defects of femoral articular cartilage: an experimental study in the goat | |
US6632651B1 (en) | Tissue maintenance system that applies rhythmic pulses of pressure | |
Almqvist et al. | Culture of chondrocytes in alginate surrounded by fibrin gel: characteristics of the cells over a period of eight weeks | |
US20070172812A1 (en) | Tissue equivalent for transplantation and method for producing same | |
JPS59192364A (ja) | 軟骨および骨の修復方法 | |
Yates et al. | Phenotypic analysis of bovine chondrocytes cultured in 3D collagen sponges: effect of serum substitutes | |
EP2302037A1 (en) | Method for non-autologous cartilage regeneration | |
Wong et al. | Facilitating in vivo articular cartilage repair by tissue-engineered cartilage grafts produced from auricular chondrocytes | |
EP3025720A1 (en) | Preparation method for therapeutic agent of bead-type chondrocyte | |
Kisiday | Expansion of chondrocytes for cartilage tissue engineering: A review of chondrocyte dedifferentiation and redifferentiation as a function of growth in expansion culture | |
Peña et al. | In vivo behavior of complete human oral mucosa equivalents: characterization in athymic mice | |
Choi et al. | Chondrogenesis of human periosteum-derived progenitor cells in atelocollagen | |
RU2016137290A (ru) | Fgf-18 в процедурах пересадки трансплантата и тканевой инженерии | |
JP7321152B2 (ja) | 軟骨移植片の再細胞化を可能にするエラスチン低減 | |
WO2002012451A1 (fr) | Procede de culture de chondrocytes humains | |
JP2012000262A (ja) | ヒト軟骨細胞と新規足場材料を用いた軟骨組織の製法 | |
JP2001293081A (ja) | 軟骨移植用材料及びその製造方法 | |
CA2254350A1 (en) | Method for bioengineering cartilage | |
WO2024024708A1 (ja) | 軟骨修復用組成物及びその製造方法 | |
Moskalewski | Chondrocytes | |
SCAFFOLDS et al. | A LIVING HYALINE CARTILAGINOUS GRAFT FOR CARTILAGE TISSUE ENGINEERING | |
Glattauer et al. | Direct use of resorbable collagen-based beads for cell delivery in tissue engineering and cell therapy applications |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20080509 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20110329 |
|
A521 | Written amendment |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20110530 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20120131 |
|
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20120221 |
|
FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20150302 Year of fee payment: 3 |
|
R150 | Certificate of patent or registration of utility model |
Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
LAPS | Cancellation because of no payment of annual fees |