JPS6398321A - Shiitake spawn and its production - Google Patents
Shiitake spawn and its productionInfo
- Publication number
- JPS6398321A JPS6398321A JP61244263A JP24426386A JPS6398321A JP S6398321 A JPS6398321 A JP S6398321A JP 61244263 A JP61244263 A JP 61244263A JP 24426386 A JP24426386 A JP 24426386A JP S6398321 A JPS6398321 A JP S6398321A
- Authority
- JP
- Japan
- Prior art keywords
- treated water
- ceramics
- culture
- bottle
- expansion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 240000000599 Lentinula edodes Species 0.000 title claims description 10
- 238000004519 manufacturing process Methods 0.000 title claims description 4
- 235000001715 Lentinula edodes Nutrition 0.000 title 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 239000000919 ceramic Substances 0.000 claims description 22
- 241000894006 Bacteria Species 0.000 claims description 11
- 230000001954 sterilising effect Effects 0.000 claims description 9
- 229920001817 Agar Polymers 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 238000010586 diagram Methods 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 4
- 238000007796 conventional method Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- 229910002090 carbon oxide Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 235000010419 agar Nutrition 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
Landscapes
- Mushroom Cultivation (AREA)
Abstract
(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。(57) [Abstract] This bulletin contains application data before electronic filing, so abstract data is not recorded.
Description
【発明の詳細な説明】
（Ａ）発明の目的について
（イ）産業上の利用分野
本発明は椎茸栽培において使用する菌種駒及びその製造
方法に関するものである。DETAILED DESCRIPTION OF THE INVENTION (A) Object of the invention (a) Field of industrial application The present invention relates to a bacterial seed piece used in shiitake mushroom cultivation and a method for producing the same.
（ロ）従来の技術について
従来椎茸菌種駒（以下「種駒」と称す。）は、生駒（１
３）とオガクズ（９）を混合し適度の水分を有せしめて
殺菌したる後拡大培養した母菌を接種して培養すること
によって種駒を製していた。(b) Regarding conventional technology, Shiitake mushroom seeds (hereinafter referred to as ``seeds'') are conventionally used in Ikoma (1
Seeds were prepared by mixing 3) and sawdust (9), adding an appropriate amount of moisture, sterilizing the mixture, inoculating the expanded cultured mother fungus, and culturing it.
（ハ）本発明が解決しようとする問題点前記のような種
駒の製造方法では、菌の組織培養及び拡大培養更には母
菌接種後の種駒培養において雑菌率が５％〜１０％と高
く、又発菌率が７０％〜８０％と低いため生駒（１３）
への活着率も８０％以下と低率であるため母菌の活性化
を図り生駒（１３）への活着率を向上すると共に原木へ
の活着良好な種駒を提供せんとするものである。(c) Problems to be solved by the present invention In the method for producing seed pieces as described above, the rate of miscellaneous bacteria is as high as 5% to 10% in the tissue culture and expansion culture of bacteria, as well as in the culture of seed pieces after mother bacteria inoculation. , and Ikoma (13) because the germination rate is low at 70% to 80%.
Since the rate of rooting on Ikoma (13) is low, at less than 80%, we aim to activate the mother bacteria and improve the rate of rooting on Ikoma (13), as well as provide seeds that have good rooting on logs.
（Ｂ）発明の構成及び作用について
本発明は以上の目的をもってなされたものであるが次に
その構成について図面に従って説明する。(B) Structure and operation of the invention The present invention has been made with the above objectives in mind, and the structure will now be explained with reference to the drawings.
第１図は椎茸菌の組織培養及び拡大培養の作用説明図で
あるが、寒天及び澱粉、ビタミン等栄養素（２′）を、
セラミックスに流液又は当接して得たセラミックス処理
水（２）で混合溶解し、これを試験管（６）に入れてセ
ラミックス処理水（２）使用の殺菌釜（３）で蒸気殺菌
して寒天培地（１）となし、この寒天培地（１）に、成
熟した椎茸から切り取った組織（４）を接種培養する。Figure 1 is an explanatory diagram of the effects of tissue culture and expansion culture of Shiitake mushrooms, and nutrients (2') such as agar, starch, and vitamins are
Mix and dissolve ceramics treated water (2) obtained by flowing or contacting ceramics, put this into a test tube (6), and steam sterilize it in a sterilization pot (3) using ceramics treated water (2) to obtain agar. The tissue (4) cut from a mature shiitake mushroom is inoculated into the agar medium (1) and cultured.
なお殺菌釜（３）には水とセラミックス（１５）を入れ
てセラミックス処理水（２）を得るのが便利である。他
方オガクズその他の栄養素からなる拡大培地材料（５）
をセラミックス処理水（２）でよく混合し全体の含水量
を５５％〜６５％程度とし、セラミックス処理水（２）
を使用した殺菌釜（３″）で殺菌して拡大培地（７）と
なし、この拡大培地（７）に前記組織培養の椎茸菌（４
′）を接種して培養室（１４）で拡大培養して母菌（８
）を成牛ずる。この際培養室（１４）の湿度を６０％〜
７０％とするのが最適であるが乾燥するようなときはセ
ラミックス処理水（２）を適宜撒水する。Note that it is convenient to obtain ceramic-treated water (2) by putting water and ceramics (15) into the sterilization pot (3). On the other hand, expansion medium material (5) consisting of sawdust and other nutrients
Mix well with ceramics-treated water (2) to make the total moisture content about 55% to 65%, and add ceramics-treated water (2).
was sterilized in a sterilizing pot (3″) using
') and expand culture in the culture room (14) to obtain mother bacteria (8
) as an adult cow. At this time, the humidity in the culture room (14) is set to 60% or more.
It is optimal to set it to 70%, but if it becomes dry, sprinkle ceramics treated water (2) as appropriate.
次に第２図は種駒の培養説明図であるが、まず生駒（１
３）をセラミックス処理水（２）の中に２４時間前後浸
漬処理して充分水を含ませる。この浸漬処理した生駒（
１３）をオガクズ（９）と混合してボトル（１０）に入
れ更にこのボトル（１０）に５５景％〜６５量％のセラ
ミックス処理水（２）を入れて撹特したる後セラミック
ス処理水（２）を使用した蒸気式の殺菌釜（１１’）で
殺菌処理する。殺菌処理は高圧の場合で１２０℃をもっ
て１時間、常圧 の場合９８℃で３時間程度行えば充分
である。Next, Figure 2 is an explanatory diagram of the culture of seed seeds.
3) is immersed in ceramic treatment water (2) for about 24 hours to fully absorb water. This soaked Ikoma (
Mix 13) with sawdust (9) and put it in a bottle (10), then add 55% to 65% of ceramics treated water (2) in this bottle (10) and stir it. 2) is sterilized using a steam-type sterilization pot (11'). For sterilization, it is sufficient to perform the sterilization treatment at 120°C for 1 hour under high pressure, or at 98°C for about 3 hours under normal pressure.
この殺菌処理したボトル（１０）（オガクズと生駒）に
前記の拡大培養した母菌（８）を接種し培養室（１２）
で培養する。この際培養室（１２）の温度は２０℃〜２
５℃、湿度６０％〜７０％、−酸化炭素濃度５００ＰＰ
Ｍ以下とするのが最適で、湿度はセラミックス処理水（
２）を撒水して調整する。然るときは培養室（１２）の
温度、湿度、−酸化炭素濃度は非常に安定する。This sterilized bottle (10) (sawdust and fresh produce) is inoculated with the expanded cultured mother bacteria (8) and moved to the culture room (12).
Cultivate with At this time, the temperature of the culture chamber (12) is between 20℃ and 20℃.
5℃, humidity 60% to 70%, -carbon oxide concentration 500PP
It is best to keep the humidity below M, and the humidity is ceramic treated water (
2) Sprinkle water and adjust. In such a case, the temperature, humidity, and carbon oxide concentration in the culture chamber (12) will be very stable.
以上のようにして培養すると３日〜４日で発菌し活着し
て種駒（１６）を得ることができる。When cultured as described above, the seeds germinate and take root in 3 to 4 days, and seed pieces (16) can be obtained.
（Ｃ）発明の効果について
以上のように組織培養、拡大培養及び種駒培養において
セラミックス処理水を使用するとそれぞれ各培養時にお
ける雑菌率が、従来の方法では５％〜１０％であるのに
たいして２％以下と低率であり、又発菌率は従来の方法
ではそれぞれ７０％〜８０％であるのにたいして９８％
以上と高率であり、更に生駒（１３）への活着率が、従
来の方法では８０％以下なのにたいして本発明による場
合は１００％に近いという大きな効果が得られる。又本
発明による種駒（１６）で植菌した原木の発菌率も１０
０％に近い成績を有するという大きな利点を有するもの
である。(C) Effects of the invention As described above, when ceramic-treated water is used in tissue culture, expansion culture, and seed culture, the bacteria rate during each culture is 2%, compared to 5% to 10% with conventional methods. % or less, and the germination rate is 98%, compared to 70% to 80% with conventional methods.
This is a high rate, and furthermore, the rate of attachment to Ikoma (13) is less than 80% in the conventional method, whereas the present invention has a great effect of being close to 100%. In addition, the germination rate of logs inoculated with seed pieces (16) according to the present invention was 10.
It has the great advantage of having a performance close to 0%.
図面は実施例を示す作用説明図で、 第１図は椎茸菌の組織培養及び拡大培養図である。 第２図は種駒の培養図である。 The drawings are action explanatory diagrams showing examples. FIG. 1 is a diagram of tissue culture and expanded culture of Shiitake fungi. Figure 2 is a diagram of the culture of seed pieces.
Claims (2)
地（１）及び拡大培地（７）をつくる場合、各培地材料
（２′）（５）の混合及び殺菌処理に使用する水はすべ
てセラミックス処理水（２）を使用して母菌（８）を生
成し、他方生駒（１３）をセラミックス処理水（２）の
中に充分浸漬したる後オガクズ（９）と共にボトル（１
０）に入れ、これに５５量％〜６５量％のセラミックス
処理水（２）を入れて撹拌したる後、セラミックス処理
水（２）を使用した蒸気式の殺菌釜（１１）で殺菌処理
しこの殺菌処理したボトル（１０）の中に前記母菌（８
）を接種し、次いでこのボトル（１０）を培養室（１２
）でセラミックス処理水（２）の撒水で湿度を調整しな
がら培養活着してなる椎茸菌種駒。(1) When making agar medium (1) and expansion medium (7) in the tissue culture and expansion culture of Shiitake mushrooms, all the water used for mixing and sterilizing each medium material (2') (5) is made of ceramics. The treated water (2) is used to generate mother bacteria (8), while the Ikoma (13) is thoroughly immersed in the ceramics treated water (2), and then the bottle (1) is prepared with sawdust (9).
0), add 55% to 65% by volume of ceramics-treated water (2) and stir, and then sterilize it in a steam-type sterilizer (11) using ceramics-treated water (2). The mother bacteria (8) are placed in this sterilized bottle (10).
), and then this bottle (10) was placed in the culture chamber (12).
), the Shiitake mushroom seeds are cultured and taken root while adjusting the humidity by sprinkling ceramic-treated water (2).
地（１）及び拡大培地（７）をつくる場合、各培地材料
（２′）（５）の混合及び殺菌処理に使用する水はすべ
てセラミックス処理水（２）を使用して母菌（８）を生
成し、他方生駒（１３）をセラミックス処理水（２）の
中に充分浸漬したる後オガクズ（９）と共にボトル（１
０）に入れ、これに５５量％〜６５量％のセラミックス
処理水（２）を入れて撹拌したる後、セラミックス処理
水（２）を使用した蒸気式の殺菌釜（１１）で殺菌処理
しこの殺菌処理したボトル（１０）の中に前記母菌（８
）を接種し、次いでこのボトル（１０）を培養室（１２
）でセラミックス処理水（２）の撒水で湿度を調整しな
がら培養活着してなることを特徴とする椎茸菌種駒の製
造方法。(2) When making agar medium (1) and expansion medium (7) in the tissue culture and expansion culture of Shiitake mushrooms, all the water used for mixing and sterilizing each medium material (2') (5) is made of ceramics. The treated water (2) is used to generate mother bacteria (8), while the Ikoma (13) is thoroughly immersed in the ceramics treated water (2), and then the bottle (1) is prepared with sawdust (9).
0), add 55% to 65% by volume of ceramics-treated water (2) and stir, and then sterilize it in a steam-type sterilizer (11) using ceramics-treated water (2). The mother bacteria (8) are placed in this sterilized bottle (10).
), and then this bottle (10) was placed in the culture chamber (12).
) A method for producing shiitake mushroom seeds, characterized in that the seeds are cultured while controlling humidity by sprinkling with ceramic-treated water (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61244263A JPS6398321A (en) | 1986-10-16 | 1986-10-16 | Shiitake spawn and its production |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61244263A JPS6398321A (en) | 1986-10-16 | 1986-10-16 | Shiitake spawn and its production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6398321A true JPS6398321A (en) | 1988-04-28 |
Family
ID=17116148
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61244263A Pending JPS6398321A (en) | 1986-10-16 | 1986-10-16 | Shiitake spawn and its production |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6398321A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02104278A (en) * | 1988-10-13 | 1990-04-17 | Akira Yamazaki | Method for culturing mycelia of basidiomycete or ascomycete |
-
1986
- 1986-10-16 JP JP61244263A patent/JPS6398321A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02104278A (en) * | 1988-10-13 | 1990-04-17 | Akira Yamazaki | Method for culturing mycelia of basidiomycete or ascomycete |
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