JPS63116624A - Bottle culture of edible mushrooms - Google Patents

Abstract

(57) [Abstract] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention] (A) Object of the invention (a) Industrial application field The present invention is an improvement in the cultivation method of edible species such as oyster mushrooms, maitake mushrooms, enoki mushrooms, etc. It's something to do with.

(b) Regarding conventional technology, bottle cultivation of these mushroom species is carried out through cell culture using an agar medium, expansion culture using an expansion medium, and inoculation cultivation using a cultivation medium. When using clean water such as well water, the present invention aims to solve
, J:5 In the conventional cultivation method, the incidence of various bacteria is high in agar culture and expansion culture of mushroom fungi, and inoculation bottle cultivation, and therefore the germination rate and survival rate (incidence rate) of cultured bacteria are 70.
The problem was that the score was low at 80.

Particularly in bottle cultivation, the germination rate and incidence are the keys to winning or losing in competition between companies, and we aim to provide all cultivation methods with improved quality.

(B') Regarding the structure of the invention The present invention has been made with the above-mentioned objectives.Next, the structure will be explained with reference to the drawings.

FIG. 1 is an explanatory diagram of tissue culture and expansion culture. First, in tissue culture, agar and starch, which are agar medium materials (2'), and nutrients such as vitamins are added to the cellulose 7. (6
Ceramics treated water obtained by redundant or contact treatment with 1 (2
), put it in a test tube (12), use ceramic treated water (2), sterilize it in a steam sterilization pot (3), and use it as an agar medium (1)! obtain.

Next, mushroom tissue pieces (4) are planted on this agar medium (1) and cultured in a culture chamber (14). (13) is a cotton plug.

Ceramic treated water (2) can be used in the sterilization pot (3).
may be used as ceramic-treated water by placing water and ceramics (6) in a sterilizing pot (3). Mushroom fungi (4) can be obtained after a culturing period of about - months.

Next, for expansion culture, the expansion medium material (5) made of sawdust, rice bran, etc. is sterilized in a sterilization pot (3') using ceramics-treated water (2) to form an expansion medium (7). Transfer the mushroom fungus (4') Y obtained through the tissue culture to the culture chamber (14') and expand the culture to obtain mother fungus (8) 7. At this time, the optimum humidity in the culture room (14') is 60% to 70%, but if it is too dry, sprinkle ceramic-treated water (2) as appropriate.

Next, Figure 2 is a diagram showing the effects of inoculation and bottle cultivation, and the cultivation medium material (5'
) with the ceramics treated water (2), mix it well, put it into the bottle (10), and add the ceramics treated water (2).
Sterilize the cultivation medium (5) using the steam sterilizer (3).
〃〃, and the above-mentioned mother fungus (8)k in the cultivation medium (5〃).
Inoculate the mushrooms and perform 2 cultivation of mushrooms (15) in the cultivation room (11). The humidity in the cultivation room (1) is usually kept at 90 degrees or higher, but humidification to maintain humidity is done using Ceraminx-treated water (2). Although it has the above configuration, its operation and effects will be explained next.

In the tissue culture of mushroom fungi, the agar medium material (2') is mixed well with ceramics-treated water (2) and boiled, then sterilized with high-pressure steam for 71 hours in ceramics-treated water (2) to form agar medium (1). , put this in a test tube (6),
Mushroom tissue piece (4)? Place the cotton plug (13) on the culture chamber (
14).

The temperature of the culture room (14) is 22°C to 24°C and the humidity is 60%.
~70%, and the CO2 concentration is 500 PPM or less.

With this method, bacteria germinated in 2 to 3 days, the bacteria had very good vitality, and the bacteria grew quickly, 2 to 3 days faster than the conventional method.

The advantages over the conventional method are shown in Table 1 below.

(Table 1) Table of effectiveness in tissue culture As shown in the table above, the hyphae have good motility, the rate of miscellaneous bacteria has decreased, and the germination rate has greatly improved.

Next, expansion culture is carried out in a large test tube or small bottle, and the water used for mixing the expansion medium material (5) is suitably 60% ceramic treated water (2). High-pressure sterilization using the sterilization pot (3') can be completed in one hour using ceramic treated water (2), and the expansion medium (7) can be heated. Transfer 4 tissue-cultured mushroom fungi to this expansion medium (7) and culture (
Cultivate at 14 µm or in a culture room (the temperature of 14 µm is 2 µm).
2℃~24℃, humidity 60%~70%, CO□ concentration 500
PPM or less, and adjust the humidity using ceramic treated water (2
).

In such cases, the effects shown in the following table can be obtained compared to the conventional method.

Table 2 Expansion culture in a large test tube (killing of the medium [♀j means high-pressure sterilization) Table 3 Expansion culture in a bottle One mother bacterium (8) obtained as described above was grown as shown in Figure 2. Although the culture medium (5') is inoculated and cultured, one hour is sufficient for sterilizing the culture medium (5') in a bottle (1o) using a Koo sterilizing pot (of course, it is not necessary to use ceramic treatment). (This is done using water (2) as described above.) It takes a longer time than the conventional method. I will inoculate (8) and put it in the cultivation room (IJ) and wait for the growth to occur.Does the cultivation management such as temperature and humidity in the cultivation room (1) differ slightly depending on the type of mushroom used?

This is almost the same as the conventional method.

However, regardless of the temperature, germination and growth will occur even if the temperature is 2°C to 4°C lower than the rice cultivation temperature.

Note that humidification to maintain humidity is performed using ceramic-treated water).

Table 4 (Crab germination temperature) As shown in this table, crabs germinate at a lower temperature than with conventional cultivation methods, and the required number of days for germination is also 7 to 10 days compared to conventional methods.
You can get the effect of being earlier in the day.

Therefore, there is an advantage in that the amount of materials to be managed is reduced, and the harvest is also faster.

Next, a comparison of the origin, yield, etc. is shown in the table below.

As shown in this table, using ceramic-treated water (2) shortens the period from outbreak to harvest and greatly increases yields.

What is particularly remarkable is that in bottle cultivation, there is a perception that the color and shape of the mushrooms produced are poor, but when grown using the method of the present invention, the color, shape, and aroma of the mushrooms are very good. Mushrooms grown using conventional cultivation methods have almost no fragrance, but mushrooms grown using the proposed method have the same aroma and color as natural mushrooms. There is a big advantage of Zukero.

[Brief explanation of the drawing]

Fig. 1 is an explanatory diagram of tissue culture and expansion culture of mushroom fungi, and Fig. 2 is an explanatory diagram of inoculation and generation using a bottle.

Claims (1)

[Claims] In the bacterial tissue culture of edible mushrooms and its expansion culture, each medium material (
2') The water used for the treatment and sterilization in (5) is all ceramics-treated water used to cultivate the mother bacteria (8), and the cultivation medium material (5') is mixed with the ceramics-treated water (2). This is then placed in a bottle (10) and steam sterilized using ceramic treated water (2) to form a cultivation medium (5'').The medium (5'') is inoculated with mother bacteria (8) and placed in the cultivation room. (11) A method for cultivating edible mushrooms in a bottle, characterized in that the humidity in the cultivation room is adjusted and maintained using ceramic-treated water.