CN107047307A - A kind of tissue method for culturing seedlings of golden pineapple - Google Patents
A kind of tissue method for culturing seedlings of golden pineapple Download PDFInfo
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- CN107047307A CN107047307A CN201710305522.6A CN201710305522A CN107047307A CN 107047307 A CN107047307 A CN 107047307A CN 201710305522 A CN201710305522 A CN 201710305522A CN 107047307 A CN107047307 A CN 107047307A
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- sprout
- tissue
- hardening
- golden pineapple
- culturing seedlings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
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- Cell Biology (AREA)
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
The invention discloses a kind of tissue method for culturing seedlings of golden pineapple, comprise the following steps：（1）Take bud,（2）Sterilization,（3）Culture,（4）Hardening,（5）Transplant.Bacterium is less than 1% to the pollution rate of tissue in this method incubation, and the final survival rate more than 90% of Tissue vaccine, obtained golden pineapple seedling individual is uniform, grows vigorous.
Description
Technical field
The invention belongs to application technical field, in particular it relates to a kind of tissue method for culturing seedlings of golden pineapple.
Background technology
Golden pineapple, i.e. MD-2 pineapples, general 1-2 kilograms of fruit weight, cylindrical shape, leaf margin is stingless, and blade elongate surface is smooth, fruit
Thin skin, eye is shallow, and pulp is golden yellow, and meat is careful, and fiber is moderate, and mouthfeel is careful, and just melt in the mouth is with rich flavor, and mouthfeel is good.Especially
It is that skin is relatively thin and peeling removes eye without dissected valley, sweet taste weight need not steep salt solution, and peeling just can directly be eaten, very convenient.Gold
Pineapple fragrance succulence, storage period is long, if under the conditions of 7.5 DEG C, can preserve up to 25 days.
Domestic pineapple area is at 1,000,000 mu, and current 2,000 mu or so of area at home of golden pineapple account for the pineapple gross area 0.2%
Left and right, present fruit chain store and supermarket almost sell, market is in backorder status, meets if desired chain without golden pineapple
Shop and the demand of supermarket, golden pineapple area need to reach 100,000 mu, and market has openings is huge.The modes of reproduction of current pineapple has seed
There is the problem of reproductive efficiency is not high in breeding and vegetative propagation, current vegetative propagation.
The content of the invention
Present invention aim to address above mentioned problem, there is provided a kind of tissue method for culturing seedlings of golden pineapple.
The purpose of the present invention is implemented by the following technical programs：
A kind of tissue method for culturing seedlings of golden pineapple, comprises the following steps：
（1）Take bud：Win the crown bud of golden pineapple or inhale bud, peel off outer blade, retain 1-2 piece tender leafs；
（2）Sterilization：Ultraviolet disinfection is carried out to superclean bench, with 70% ethanol opponent and inoculating facility cleaning disinfection, then will be walked
Suddenly（1）Sprout after processing is placed in superclean bench, and sprout is sterilized with 70% ethanol, is rinsed 2-3 times with water, then with sterilization
Agent carries out disinfection, then is rinsed 3-4 times with water, then cuts sprout, obtains sprout tuber；
（3）Culture：By step（2）Obtained sprout tuber is put into progress tissue training in the blake bottle equipped with root media by size
Support, the sprout tuber sizableness in each blake bottle is taken root to sprout tuber, obtains Tissue vaccine；
（4）Hardening：By step（3）Obtained Tissue vaccine moves to outdoor carry out hardening；
（5）Transplant：By step（4）Tissue transplantation of seedlings after hardening carries out normal management into the matrix sterilized.
Further, the step（2）In, time >=20min of ultraviolet disinfection, 70% ethanol are carried out to superclean bench
It is 10-15s to the time that sprout sterilizes, the disinfectant is 2-3wt% copper-baths, and disinfectant disinfecting time is 11-
13min, each sprout cuts into 4 pieces.
Further, the step（3）In, sprout tuber 3-5 is put into each blake bottle, the root media is 1/
2MS+ sucrose+agar, the sucrose and agar account for the 3.5% and 0.5% of root media weight respectively, and condition of culture is：Culture
29-32 DEG C of temperature, daily illumination 7-8h.
Further, the step（4）In, hardening time when carrying out outdoor hardening is 7-10d, and shade density is 50-
60%。
Further, the step（5）In, the matrix is 1 by weight ratio:1:1:1 vermiculite, perlite, plant ash
With peat soil composition, handled before vermiculite, perlite, plant ash and peat soil use through steam sterilizing.
Golden eyelet stitch's method for culturing seedlings of the present invention includes taking bud, sterilization, culture, hardening, transplanting process, and sterilization is reduced
Pollution of the bacterium to tissue in incubation, condition of culture and culture matrix rationally, can accelerate tissue and take root.The side of the present invention
Method, which is realized, has the advantages that survival rate is high and reproductive efficiency is high.
The invention has the advantages that：
Bacterium is less than 1%, Tissue vaccine to the pollution rate of tissue in golden eyelet stitch's method for culturing seedlings that the present invention is provided, incubation
Final survival rate more than 90%, obtained golden pineapple seedling individual is uniform, grows vigorous.
Embodiment
Embodiment 1
A kind of tissue method for culturing seedlings of golden pineapple, comprises the following steps：
（1）Take bud：Win the crown bud of golden pineapple or inhale bud, peel off outer blade, retain 1-2 piece tender leafs.
（2）Sterilization：Ultraviolet disinfection is carried out to superclean bench, disinfecting time >=20min is then shut off uviol lamp.With 70%
Ethanol opponent and inoculating facility cleaning disinfection, are then placed in superclean bench by the sprout after processing, and sprout is disappeared with 70% ethanol
Malicious 12s, is rinsed 3 times, then carried out disinfection 12min with 2.5wt% copper-baths with water, then is rinsed 4 times with water, then by bud
Body carries out cutting into 4 pieces.
（3）Culture：Sprout tuber is put into by size in the blake bottle equipped with root media and carries out tissue cultures, culture of rootage
Base is 1/2MS+ sucrose+agar, and sucrose and agar are accounted in the 3.5% and 0.5% of root media weight, each blake bottle respectively
Sprout tuber sizableness, every bottle larger of sprout tuber puts 3, and less every bottle of sprout tuber puts 5, is cultivated under the conditions of 30 DEG C, often
Day illumination 8h, taken root to sprout tuber, obtain Tissue vaccine.
（4）Hardening：Tissue vaccine is moved into outdoor carry out hardening, the hardening time is 8d, and shade density is 55%.
（5）Transplant：Tissue transplantation of seedlings after hardening is extremely compared 1 by weight:1:1:1 vermiculite, perlite, plant ash and mud
Charcoal local soil type into matrix in, carry out normal management, carry out normal management, vermiculite, perlite, plant ash and mud in the matrix of sterilizing
Handled before charcoal soil use through steam sterilizing.
Bacterium is 0.7% to the pollution rate of sprout tuber in the present embodiment culture, and the survival rate of Tissue vaccine is 95%.
Embodiment 2
A kind of tissue method for culturing seedlings of golden pineapple, comprises the following steps：
（1）Take bud：Win the crown bud of golden pineapple or inhale bud, peel off outer blade, retain 1-2 piece tender leafs.
（2）Sterilization：Ultraviolet disinfection is carried out to superclean bench, disinfecting time >=20min is then shut off uviol lamp.With 70%
Ethanol opponent and inoculating facility cleaning disinfection, are then placed in superclean bench by the sprout after processing, and sprout is disappeared with 70% ethanol
Malicious 10s, is rinsed 2 times, then carried out disinfection 11min with 3wt% copper-baths with water, then is rinsed 4 times with water, then by sprout
Progress cuts into 4 pieces.
（3）Culture：Sprout tuber is put into by size in the blake bottle equipped with root media and carries out tissue cultures, culture of rootage
Base is 1/2MS+ sucrose+agar, and sucrose and agar are accounted in the 3.5% and 0.5% of root media weight, each blake bottle respectively
Sprout tuber sizableness, every bottle larger of sprout tuber puts 3, and less every bottle of sprout tuber puts 5, is cultivated under the conditions of 29 DEG C, often
Day illumination 8h, taken root to sprout tuber, obtain Tissue vaccine.
（4）Hardening：Tissue vaccine is moved into outdoor carry out hardening, the hardening time is 7d, and shade density is 50%.
（5）Transplant：Tissue transplantation of seedlings after hardening is extremely compared 1 by weight:1:1:1 vermiculite, perlite, plant ash and mud
Charcoal local soil type into matrix in, carry out normal management, carry out normal management, vermiculite, perlite, plant ash and mud in the matrix of sterilizing
Handled before charcoal soil use through steam sterilizing.
Bacterium is 0.6% to the pollution rate of sprout tuber in the present embodiment culture, and the survival rate of Tissue vaccine is 91%.
Embodiment 3
A kind of tissue method for culturing seedlings of golden pineapple, comprises the following steps：
（1）Take bud：Win the crown bud of golden pineapple or inhale bud, peel off outer blade, retain 1-2 piece tender leafs.
（2）Sterilization：Ultraviolet disinfection is carried out to superclean bench, disinfecting time >=20min is then shut off uviol lamp.With 70%
Ethanol opponent and inoculating facility cleaning disinfection, are then placed in superclean bench by the sprout after processing, and sprout is disappeared with 70% ethanol
Malicious 15s, is rinsed 3 times, then carried out disinfection 13min with 2wt% copper-baths with water, then is rinsed 3 times with water, then by sprout
Progress cuts into 4 pieces.
（3）Culture：Sprout tuber is put into by size in the blake bottle equipped with root media and carries out tissue cultures, culture of rootage
Base is 1/2MS+ sucrose+agar, and sucrose and agar are accounted in the 3.5% and 0.5% of root media weight, each blake bottle respectively
Sprout tuber sizableness, every bottle larger of sprout tuber puts 3, and less every bottle of sprout tuber puts 5, is cultivated under the conditions of 32 DEG C, often
Day illumination 7h, taken root to sprout tuber, obtain Tissue vaccine.
（4）Hardening：Tissue vaccine is moved into outdoor carry out hardening, the hardening time is 10d, and shade density is 60%.
（5）Transplant：Tissue transplantation of seedlings after hardening is extremely compared 1 by weight:1:1:1 vermiculite, perlite, plant ash and mud
Charcoal local soil type into matrix in, carry out normal management, carry out normal management, vermiculite, perlite, plant ash and mud in the matrix of sterilizing
Handled before charcoal soil use through steam sterilizing.
Bacterium is 0.8% to the pollution rate of sprout tuber in the present embodiment culture, and the survival rate of Tissue vaccine is 92%.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, although with reference to foregoing reality
Apply example the present invention is described in detail, for those skilled in the art, it still can be to foregoing each implementation
Technical scheme described in example is modified, or carries out equivalent substitution to which part technical characteristic.All essences in the present invention
God is with principle, and any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (5)
1. a kind of tissue method for culturing seedlings of golden pineapple, it is characterised in that comprise the following steps：
（1）Take bud：Win the crown bud of golden pineapple or inhale bud, peel off outer blade, retain 1-2 piece tender leafs；
（2）Sterilization：Ultraviolet disinfection is carried out to superclean bench, with 70% ethanol opponent and inoculating facility cleaning disinfection, then will be walked
Suddenly（1）Sprout after processing is placed in superclean bench, and sprout is sterilized with 70% ethanol, is rinsed 2-3 times with water, then with sterilization
Agent carries out disinfection, then is rinsed 3-4 times with water, then cuts sprout, obtains sprout tuber；
（3）Culture：By step（2）Obtained sprout tuber is put into progress tissue training in the blake bottle equipped with root media by size
Support, the sprout tuber sizableness in each blake bottle is taken root to sprout tuber, obtains Tissue vaccine；
（4）Hardening：By step（3）Obtained Tissue vaccine moves to outdoor carry out hardening；
（5）Transplant：By step（4）Tissue transplantation of seedlings after hardening carries out normal management into the matrix sterilized.
2. the tissue method for culturing seedlings of golden pineapple according to claim 1, it is characterised in that the step（2）In, to ultra-clean
Workbench carries out time >=20min of ultraviolet disinfection, and the time that 70% ethanol is sterilized to sprout is 10-15s, and the disinfectant is
2-3wt% copper-baths, disinfectant disinfecting time is 11-13min, and each sprout cuts into 4 pieces.
3. the tissue method for culturing seedlings of golden pineapple according to claim 1, it is characterised in that the step（3）In, Mei Gepei
Support and sprout tuber 3-5 is put into bottle, the root media is 1/2MS+ sucrose+agar, and the sucrose and agar are accounted for respectively takes root
The 3.5% of culture medium weight and 0.5%, condition of culture is：29-32 DEG C of cultivation temperature, daily illumination 7-8h.
4. the tissue method for culturing seedlings of golden pineapple according to claim 1, it is characterised in that the step（4）In, carry out room
Hardening time during outer hardening is 7-10d, and shade density is 50-60%.
5. the tissue method for culturing seedlings of golden pineapple according to claim 1, it is characterised in that the step（5）In, the base
Matter is 1 by weight ratio:1:1:1 vermiculite, perlite, plant ash and peat soil composition, vermiculite, perlite, plant ash and peat
Handled before native use through steam sterilizing.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710305522.6A CN107047307A (en) | 2017-05-03 | 2017-05-03 | A kind of tissue method for culturing seedlings of golden pineapple |
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| CN201710305522.6A CN107047307A (en) | 2017-05-03 | 2017-05-03 | A kind of tissue method for culturing seedlings of golden pineapple |
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| CN107047307A true CN107047307A (en) | 2017-08-18 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110012834A (en) * | 2019-04-02 | 2019-07-16 | 云南省热带作物科学研究所 | A method of improving golden eyelet stitch culture efficiency |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101371635A (en) * | 2007-08-20 | 2009-02-25 | 何光浚 | Method of cottage and breeding for caxillary bud of pineapple in-vitro |
| CN103385169A (en) * | 2013-07-04 | 2013-11-13 | 中国热带农业科学院热带作物品种资源研究所 | Liquid cuttage method for pineapple single leaf |
| CN104542275A (en) * | 2014-10-23 | 2015-04-29 | 重庆翰锋元浩食品有限公司 | Rapid propagation cultivation method for milk pineapple |
-
2017
- 2017-05-03 CN CN201710305522.6A patent/CN107047307A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101371635A (en) * | 2007-08-20 | 2009-02-25 | 何光浚 | Method of cottage and breeding for caxillary bud of pineapple in-vitro |
| CN103385169A (en) * | 2013-07-04 | 2013-11-13 | 中国热带农业科学院热带作物品种资源研究所 | Liquid cuttage method for pineapple single leaf |
| CN104542275A (en) * | 2014-10-23 | 2015-04-29 | 重庆翰锋元浩食品有限公司 | Rapid propagation cultivation method for milk pineapple |
Non-Patent Citations (2)
| Title |
|---|
| S.R.W.M.C.J.K.RANAWANA ET AL.: "Effects of Type and Size of Stem Cutting and Propagation Media for Rapid Multiplication of Pineapple(Ananas comosus)", 《TROPICAL AGRICULTURAL RESEARCH》 * |
| 刘胜辉等: ""MD-2"菠萝冠芽叶芽扦插技术研究", 《中国热带农业》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110012834A (en) * | 2019-04-02 | 2019-07-16 | 云南省热带作物科学研究所 | A method of improving golden eyelet stitch culture efficiency |
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Application publication date: 20170818 |
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