CN101371635A - Method of cottage and breeding for caxillary bud of pineapple in-vitro - Google Patents
Method of cottage and breeding for caxillary bud of pineapple in-vitro Download PDFInfo
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- CN101371635A CN101371635A CNA2007101470470A CN200710147047A CN101371635A CN 101371635 A CN101371635 A CN 101371635A CN A2007101470470 A CNA2007101470470 A CN A2007101470470A CN 200710147047 A CN200710147047 A CN 200710147047A CN 101371635 A CN101371635 A CN 101371635A
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- pineapple
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Abstract
The invention relates to a pineapple axillary bud in vitro cutting propagation method which is characterized in that the pineapple axillary buds are sheared, and then are rooted on a seedling bed; the soil is kept moist every day after the axillary buds are rooted, the seedling environment temperature is controlled at 18 to 35 DEG C till the pineapple seedlings are grown out. The propagation method has little investment, the technique is easy to master, 20 to 30 axillary buds can be obtained from one pineapple crown bud, each axillary bud can be cultivated into a pineapple plant, so the propagation rate is high, and the propagation method can meet the requirements of mass production.
Description
One. technical field
The invention belongs to agricultural technology field, particularly relate to the improvement of the method for caxillary bud of pineapple in-vitro cottage propagation.
Two. background technology
Pineapple (Ananas comosus) has another name called yellow pears, is that the Ananas pineapple family is often given birth to evergreen draft fruit plant for many years.The pineapple growth rapidly, and is with short production cycle, is the fruit kind that economic worth is arranged very much.Two kinds of methods of the current employing of the breeding of pineapple: the one, propagation by division is carried out in device palaces such as the crown bud of employing pineapple, holder bud, suction bud, this method, because of a pineapple plant has only totally 5 to 6 buds such as crown bud, holder bud, suction bud, the breeding multiplying power is not high, does not satisfy mass-produced needs.Second method is to adopt tissue culture technique to grow seedlings, this method, though can satisfy mass-produced requirement, the specification requirement height, investment is big, the seedling cost height, it is big to promote difficulty.
Three. summary of the invention
The objective of the invention is to design the method for caxillary bud of pineapple in-vitro cottage propagation, to overcome the crown bud, the holder bud that adopt pineapple, to inhale device palaces such as bud and carry out the propagation by division method, because of a pineapple plant has only totally 5 to 6 buds such as crown bud, holder bud, suction bud, the breeding multiplying power is not high, and adopt tissue culture technique to grow seedlings, and the specification requirement height, investment is big, the seedling cost height is promoted the big shortcoming of difficulty.
The object of the present invention is achieved like this: method of cottage and breeding for caxillary bud of pineapple in-vitro, it is characterized in that: the axillalry bud with pineapple strips earlier, then with axillalry bud in the seedbed cuttage, after the axillalry bud cuttage, keep ground moistening every day, the environment of growing seedlings control temperature is 18---and 35 degrees centigrade, until growing the pineapple seedling.Axillalry bud is in the seedbed cuttage, the cuttage degree of depth 5, and---between 40 millimeters, with 15---20 millimeters for well.The environment of growing seedlings control temperature at 2528 degrees centigrade for well.
Take the present invention of above measure, small investment, technology is grasped easily, and a pineapple crown bud just can be obtained 20 to 30 axillalry buds, and each axillalry bud just can be bred a pineapple plant, therefore breeds the multiplying power height, can satisfy mass-produced requirement.
Below the present invention is described in further detail again.
Four. embodiment
Method of cottage and breeding for caxillary bud of pineapple in-vitro is: earlier the axillalry bud with pineapple strips, then with axillalry bud in the seedbed cuttage, the cuttage degree of depth 5---between 40 millimeters, after the axillalry bud cuttage, keep ground moistening every day, the environment of growing seedlings control temperature is 18---and 35 degrees centigrade, until growing the pineapple seedling.
Concrete implement when of the present invention can be by following step:
One. the mattress sterilization of going out of crown bud: cut off crown bud from the pineapple fruit, put in 20 percent the limewash and soak the mattress sterilization of going out in 5 minutes.
Two. stripping of axillalry bud: axillalry bud is exsomatized along the periphery of pineapple crown bud stem with sharp scalpel or pocket knife and to cut off, select the healthy and strong axillalry bud of hindering that do not have, eliminate the axillalry bud of damage.
Three. the short root of axillalry bud is handled: the robust lossless of selecting is hindered band axil bud put in centesimal potent No. 1 taking root liquid of taking root, soaks taking-up after 15 minutes.
Four. the preparation in seedbed: select the leeward ventilative good sandy loam that faces south, play the smooth seedbed that becomes of furrow.
Five. the seedbed cuttage of axillalry bud: the axillalry bud cuttage that will soak in taking root liquid is on the seedbed, and the cuttage degree of depth 5---between 40 millimeters, with 15---is advisable between 20 millimeters.After the axillalry bud cuttage, keep ground moistening every day, the environment of growing seedlings control temperature is 18---and 35 degrees centigrade, with 25---be advisable for 26 degrees centigrade, after 16 to 20 days, grow the pineapple seedling.
Claims (3)
1. method of cottage and breeding for caxillary bud of pineapple in-vitro is characterized in that: the axillalry bud with pineapple strips earlier, then with axillalry bud in the seedbed cuttage, after the axillalry bud cuttage, keep ground moistening every day, the environment of growing seedlings control temperature is 18---and 35 degrees centigrade, until growing the pineapple seedling.
2. method of cottage and breeding for caxillary bud of pineapple in-vitro according to claim 1 is characterized by: described axillalry bud is in the seedbed cuttage, and 20 millimeters of the cuttage degree of depth 5---between 40 millimeters, with 15---are for well.
3. method of cottage and breeding for caxillary bud of pineapple in-vitro according to claim 1 is characterized by: the described environment control temperature of growing seedlings is 25---and 28 degrees centigrade for well.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101470470A CN101371635A (en) | 2007-08-20 | 2007-08-20 | Method of cottage and breeding for caxillary bud of pineapple in-vitro |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101470470A CN101371635A (en) | 2007-08-20 | 2007-08-20 | Method of cottage and breeding for caxillary bud of pineapple in-vitro |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101371635A true CN101371635A (en) | 2009-02-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007101470470A Pending CN101371635A (en) | 2007-08-20 | 2007-08-20 | Method of cottage and breeding for caxillary bud of pineapple in-vitro |
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| CN (1) | CN101371635A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103416203A (en) * | 2013-08-09 | 2013-12-04 | 镇江瑞繁农艺有限公司 | Method for axillary bud cutting seedling of common head cabbage |
| CN103609313A (en) * | 2013-12-04 | 2014-03-05 | 中国热带农业科学院南亚热带作物研究所 | Pineapple leaf bud cuttage method |
| CN104322250A (en) * | 2014-10-14 | 2015-02-04 | 深圳文科园林股份有限公司 | Efficient propagation method for maintaining chimera pineapple character stable inheritance |
| CN106258886A (en) * | 2016-08-15 | 2017-01-04 | 北京市农林科学院 | A kind of fine sequence awns cuttage breeding method |
| CN107047032A (en) * | 2017-05-16 | 2017-08-18 | 北海康维生态农业科技有限公司 | A kind of implantation methods of golden pineapple |
| CN107047307A (en) * | 2017-05-03 | 2017-08-18 | 北海康维生态农业科技有限公司 | A kind of tissue method for culturing seedlings of golden pineapple |
| CN107148867A (en) * | 2017-05-16 | 2017-09-12 | 北海康维生态农业科技有限公司 | A kind of seedling process method of golden pineapple |
| CN107182476A (en) * | 2017-05-05 | 2017-09-22 | 北海康维生态农业科技有限公司 | The method for culturing seedlings of golden pineapple |
| CN107232037A (en) * | 2017-05-05 | 2017-10-10 | 北海康维生态农业科技有限公司 | The breeding method of golden pineapple |
| CN115623963A (en) * | 2022-12-21 | 2023-01-20 | 海南伯特生态休闲农业科技有限公司 | Method for inducing field planted pineapple to quickly breed bud seedlings |
-
2007
- 2007-08-20 CN CNA2007101470470A patent/CN101371635A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103416203A (en) * | 2013-08-09 | 2013-12-04 | 镇江瑞繁农艺有限公司 | Method for axillary bud cutting seedling of common head cabbage |
| CN103416203B (en) * | 2013-08-09 | 2015-12-02 | 镇江瑞繁农艺有限公司 | The method of a kind of head cabbage axil bud cutting nursery |
| CN103609313A (en) * | 2013-12-04 | 2014-03-05 | 中国热带农业科学院南亚热带作物研究所 | Pineapple leaf bud cuttage method |
| CN104322250A (en) * | 2014-10-14 | 2015-02-04 | 深圳文科园林股份有限公司 | Efficient propagation method for maintaining chimera pineapple character stable inheritance |
| CN106258886A (en) * | 2016-08-15 | 2017-01-04 | 北京市农林科学院 | A kind of fine sequence awns cuttage breeding method |
| CN107047307A (en) * | 2017-05-03 | 2017-08-18 | 北海康维生态农业科技有限公司 | A kind of tissue method for culturing seedlings of golden pineapple |
| CN107182476A (en) * | 2017-05-05 | 2017-09-22 | 北海康维生态农业科技有限公司 | The method for culturing seedlings of golden pineapple |
| CN107232037A (en) * | 2017-05-05 | 2017-10-10 | 北海康维生态农业科技有限公司 | The breeding method of golden pineapple |
| CN107047032A (en) * | 2017-05-16 | 2017-08-18 | 北海康维生态农业科技有限公司 | A kind of implantation methods of golden pineapple |
| CN107148867A (en) * | 2017-05-16 | 2017-09-12 | 北海康维生态农业科技有限公司 | A kind of seedling process method of golden pineapple |
| CN115623963A (en) * | 2022-12-21 | 2023-01-20 | 海南伯特生态休闲农业科技有限公司 | Method for inducing field planted pineapple to quickly breed bud seedlings |
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Open date: 20090225 |