JPH02286020A - Massive multiplication of allium victorialis l. - Google Patents
Massive multiplication of allium victorialis l.Info
- Publication number
- JPH02286020A JPH02286020A JP1107217A JP10721789A JPH02286020A JP H02286020 A JPH02286020 A JP H02286020A JP 1107217 A JP1107217 A JP 1107217A JP 10721789 A JP10721789 A JP 10721789A JP H02286020 A JPH02286020 A JP H02286020A
- Authority
- JP
- Japan
- Prior art keywords
- stems
- garlic
- adventitious buds
- phosphorus
- inducing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000161286 Allium victorialis Species 0.000 title abstract 4
- 235000011644 Allium victorialis Nutrition 0.000 title abstract 4
- 238000012258 culturing Methods 0.000 claims abstract description 25
- 229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims abstract description 6
- 229930006000 Sucrose Natural products 0.000 claims abstract description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 5
- 239000005720 sucrose Substances 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 44
- 240000002234 Allium sativum Species 0.000 claims description 29
- 235000004611 garlic Nutrition 0.000 claims description 29
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 23
- 239000011574 phosphorus Substances 0.000 claims description 23
- 229910052698 phosphorus Inorganic materials 0.000 claims description 23
- 230000001939 inductive effect Effects 0.000 claims description 19
- 241000304531 Allium macrostemon Species 0.000 claims description 11
- 230000001902 propagating effect Effects 0.000 claims description 8
- 230000005059 dormancy Effects 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- 239000002609 medium Substances 0.000 description 45
- 238000004519 manufacturing process Methods 0.000 description 7
- 238000011160 research Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 239000006870 ms-medium Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 4
- 230000006698 induction Effects 0.000 description 4
- 238000004161 plant tissue culture Methods 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- 239000000122 growth hormone Substances 0.000 description 3
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 3
- 229960001669 kinetin Drugs 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 239000003375 plant hormone Substances 0.000 description 3
- 230000035755 proliferation Effects 0.000 description 3
- 239000005971 1-naphthylacetic acid Substances 0.000 description 2
- IIDAJRNSZSFFCB-UHFFFAOYSA-N 4-amino-5-methoxy-2-methylbenzenesulfonamide Chemical compound COC1=CC(S(N)(=O)=O)=C(C)C=C1N IIDAJRNSZSFFCB-UHFFFAOYSA-N 0.000 description 2
- 229930192334 Auxin Natural products 0.000 description 2
- XTEGARKTQYYJKE-UHFFFAOYSA-M Chlorate Chemical compound [O-]Cl(=O)=O XTEGARKTQYYJKE-UHFFFAOYSA-M 0.000 description 2
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 2
- 229910002651 NO3 Inorganic materials 0.000 description 2
- 244000184734 Pyrus japonica Species 0.000 description 2
- -1 ammonium ions Chemical class 0.000 description 2
- 239000002363 auxin Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 2
- 239000004062 cytokinin Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 1
- 239000005972 6-Benzyladenine Substances 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 244000223014 Syzygium aromaticum Species 0.000 description 1
- 235000016639 Syzygium aromaticum Nutrition 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000003898 horticulture Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000003617 indole-3-acetic acid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 150000003017 phosphorus Chemical class 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、ギヨウジヤニンニクの増殖方法に関し、詳し
くは、ギヨウジヤニンニクのリン茎の大量増殖方法、ギ
ヨウジヤニンニクの不定芽の誘導方法、及びギヨウジヤ
ニンニクのリン茎の誘導方法に関する。[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a method for propagating Japanese garlic, and more specifically, a method for mass-propagating lin stems of Japanese garlic, a method for inducing adventitious buds of Japanese garlic, The present invention also relates to a method for inducing lin stems of Japanese garlic.
ギヨウジヤニンニクの山菜としての需要は近年急激に増
大しているが、その栽培技術は確立されておらず之一部
、山地に自生している株を掘り上げて圃場に栽植するこ
とが実施されているが、需要を充分溝たすまでには至っ
ておらず、自生株の乱獲、資源枯渇が問題となっていた
。The demand for Giyojia garlic as a wild vegetable has increased rapidly in recent years, but the cultivation technology has not been established, and some methods have been carried out, such as digging up stocks that grow naturally in mountains and planting them in fields. However, this has not been enough to meet demand, and over-harvesting of native stocks and resource depletion have become problems.
これらの問題を解決する手段としては、「ギヨウジヤニ
ンニクの栽培に関する基礎的研究（第２報）種子の形成
と発芽について」金沢俊彦、へ鍬利部北海道大農学部園
芸学会昭和６３年度春季講演要旨、 Ｐ３３２〜３（１
９８Ｂ）に示される様に、種子繁殖法の確立を目指し研
究が進められており、完熟期の採種直後の種子が発芽率
が最も高いことが見出されており、種子繁殖法の可能性
が明らかになってきている。As a means to solve these problems, "Basic research on the cultivation of Giyojia garlic (2nd report) Seed formation and germination" by Toshihiko Kanazawa, Kuwaribe Hokkaido University Faculty of Agriculture Horticulture Society 1988 Spring Lecture Abstracts , P332-3(1
As shown in 98B), research is underway to establish a seed propagation method, and it has been found that seeds immediately after harvesting at full ripeness have the highest germination rate, indicating the possibility of seed propagation. It's becoming clear.
また、近年の植物組織培養技術の発展により「遺産植物
資源の食品素材化と加工利用に関する研究（１）」田中
常雄、山内順−（北海道立工業試験場）西村弘行、水谷
純也、へ鍬利部、原田隆、中嶋博（北海道大学農学部）
昭和６１年度試験研究機関共同研究報告書 Ｐ１〜１４
（１９８７）に示される様に、植物組織培養を利用した
栄養繁殖による種苗生産の可能性が見出されている。In addition, due to the recent development of plant tissue culture technology, "Research on the conversion and processing of heritage plant resources into food materials (1)" by Tsuneo Tanaka, Jun Yamauchi (Hokkaido Industrial Research Institute), Hiroyuki Nishimura, Junya Mizutani, and Ribe Kuwari. , Takashi Harada, Hiroshi Nakajima (Faculty of Agriculture, Hokkaido University)
1985 Experimental Research Institute Joint Research Report P1-14
(1987), the possibility of producing seedlings by vegetative propagation using plant tissue culture has been discovered.
前記の種子繁殖による種苗生産方法は、たとえ高い発芽
率が確保されたとしても、次に種子が採取できる成株に
なるまでに、３〜４年間の長期を必要とするため、大量
の種苗を生産するには、広大な圃場が必要となり、効率
的に安価なギヨウジヤニンニクの種苗を生産する方法と
はなりえない。Even if a high germination rate is achieved in the seed production method using seed propagation described above, it takes a long period of 3 to 4 years for the plant to become an adult plant from which seeds can be harvested, so a large number of seeds and seedlings must be produced. For production, a vast field is required, and this method cannot be used to efficiently produce inexpensive seeds and seedlings of Giyoja garlic.
また、ギヨウジヤニンニクの外植片を培養することによ
り種苗を生産する方法は、第１に、寒天などの固定培地
による大量増殖法では、その増殖率に限界があり、大量
の種苗を確保するためには数多くの試験管を使用して培
養する必要があり、極めて効率が悪く数多くの作業要員
が必要となることを意味する。固定培地を使用する組織
培養技術により生産されているウィルスウリ−苗のコス
トの６０〜７０％を人件費が占めているため、極めてコ
ストの高い苗となり普及率が非常に低い原因となってい
る。そのため、不定芽の増殖率を従来のレベルに比較し
て大幅に向上させる必要がある。In addition, the method of producing seeds and seedlings by culturing explants of Giyojia garlic requires, firstly, a large-scale propagation method using a fixed medium such as agar, which has a limit to its multiplication rate, so it is difficult to secure a large amount of seeds and seedlings. In order to do this, it is necessary to use a large number of test tubes for culturing, which means that the efficiency is extremely low and a large number of workers are required. Labor costs account for 60 to 70% of the cost of Virus Uri seedlings, which are produced using tissue culture technology using a fixed medium, making the seedlings extremely expensive and the reason for their very low penetration rate. . Therefore, it is necessary to significantly improve the proliferation rate of adventitious buds compared to the conventional level.
第２に、生産した組織培養菌は、実質上長期保存は不可
能である。そのため市場の需要に合せた生産調整を行な
うことが極めて困難であるという問題があるため、その
苗販売事業が極めて不安定な状態を余儀なくされていた
。Second, the produced tissue culture bacteria cannot be stored for a long period of time. As a result, it is extremely difficult to adjust production to match market demand, which has forced the seedling sales business to be extremely unstable.
本発明の目的はギヨウジヤニンニクの種苗を高品質に、
大量にかつ簡易に生産することにある。The purpose of the present invention is to produce high quality Giyojia garlic seeds and seedlings.
The aim is to produce in large quantities and easily.
上記目的を達成するために、本発明のリン茎の大量増殖
方法は、ギヨウジヤニンニクの外植片を培養することに
より不定芽を誘導し、必要に応じて前記不定芽を増殖さ
せた後、該不定芽を個々に分割し、培養して発根したシ
ュートを得、ついでこのシュートの根部にリン茎を形成
せしめることによりギヨウジヤニンニクのリン茎を大量
に増殖させることを特徴とするものである。In order to achieve the above object, the method for mass propagation of lin stems of the present invention involves inducing adventitious buds by culturing explants of Giyoja garlic, propagating the adventitious buds as necessary, and then The method is characterized in that the adventitious buds are individually divided, cultured to obtain rooted shoots, and then phosphorus stems are formed at the roots of these shoots, thereby propagating a large amount of phosphorus stems of Japanese garlic. be.
また、本発明のギヨウジヤニンニクの不定芽誘導方法は
、ギヨウジヤニンニクの茎頂を、リンスマイヤー−スク
ーグ培地またはムラシゲ−スクーグ培地の無機塩類濃度
１．２倍以上に希釈した無機塩類組成を有し、かつシ＝
ｌＩ！を１．０〜３．０重量％の濃度で含有する培地に
おいて培養することにより前記茎頂より不定芽を誘導す
ることを特徴とする特
また、上記方法としては、ギヨウジヤニンニクの茎頂を
、リンスマイヤー−スクーグ培地またはムラシゲ−スク
ーグ培地の無機塩［４度を１．２倍以上に希釈した無機
塩類組成を有し、かつＮＨ，＋／ＮＯ３−のモル比をＯ
，１〜０．５の範囲に修正した液体培地に培養すること
により前記茎頂より不定芽を誘導することを特徴とする
ギヨウジヤニンニクの不定芽誘導方法も提供される。In addition, the method for inducing adventitious buds of Japanese garlic of the present invention has an inorganic salt composition obtained by diluting the shoot apex of Japanese garlic to an inorganic salt concentration of 1.2 times or more of a Linsmeyer-Skoog medium or a Murashige-Skoog medium. Shi, Katsushi =
lI! The above-mentioned method is characterized in that adventitious buds are induced from the shoot apex by culturing in a medium containing 1.0 to 3.0 wt% of , an inorganic salt composition of Linsmeyer-Skoog medium or Murashige-Skoog medium [4 times diluted to 1.2 times or more, and the molar ratio of NH,+/NO3- is O
There is also provided a method for inducing adventitious buds of garlic, which comprises inducing adventitious buds from the shoot apex by culturing in a liquid medium adjusted to a range of 1 to 0.5.
また、本発明のギヨウジヤニンニクのリン茎の誘導方法
は、ギヨウジヤニンニクにおいて、シュートを明照明下
、高温にて培養を行なうことにより、前記シュートの根
部にリン茎を誘導することを特徴とする。また、本発明
によれば、前記リン茎の大量増殖方法によって得られた
リン茎を、休眠を打破し、さらに馴化させて種苗とする
ことによりギヨウジヤニンニクの種苗を大量に増殖する
ことができる。Furthermore, the method for inducing phosphorus stems of Japanese garlic according to the present invention is characterized by inducing phosphorus stems to the roots of the shoots of Japanese garlic by culturing the shoots under bright lighting and at high temperature. do. Further, according to the present invention, the seeds and seedlings of Giyoja garlic can be propagated in large quantities by breaking the dormancy of the phosphorus stems obtained by the method for mass propagation of phosphorus stems, and further acclimatizing them to make seedlings. .
本発明方法を第１図のギヨウジヤニンニク種苗生産シス
テム概略図にそって具体的に説明する。The method of the present invention will be specifically explained with reference to the schematic diagram of a garlic seedling production system shown in FIG.
本発明で用いるギヨウジヤニンニクの外植片としては、
茎頂が好ましく、ギヨウジヤニンニクリン茎１よりクリ
ーンベンチ内で無菌内に茎頂部２を摘出する。茎頂部と
しては、大きさ２００〜５００μ程度、葉原基１〜２枚
程度がついたものが適切である。The explants of Giyoja garlic used in the present invention include:
The shoot apex is preferable, and the shoot apex 2 is extracted from the Spermia japonica stem 1 in a clean bench in a sterile manner. A suitable stem apex is one with a size of about 200 to 500 μm and about 1 to 2 leaf primordia.
本発明のリン茎の大量増殖方法においては、まずギヨウ
ジヤニンニクの茎、頂等の外植片を培養することにより
不定芽３を誘導するものである。In the method for mass propagation of phosphorus stems of the present invention, adventitious buds 3 are induced by first culturing explants such as the stems and tops of Garlic japonica.
この不定芽３の誘導方法としては、茎頂２をリンスマイ
ヤー−スクーグ培地（Ｌｉｎｓｍａｔｅｒ−５ｋｏｏｇ
培地、以下ＬＳ培地という）またはムラシゲ−スクーグ
培地（Ｍｕｒａｓｈｉｇｅ−Ｓｋｏｏｇ培地、以下ＭＳ
培地という）を１．２倍以上、に希釈し、シーｒ ＩＪ
！を１．０〜３．０重量％の濃度で含有する培地におい
て培養する方法が特に好ましい。As a method for inducing these adventitious buds 3, the shoot apex 2 is grown on Linsmaier-Skoog medium (Linsmater-5koog medium).
Medium (hereinafter referred to as LS medium) or Murashige-Skoog medium (hereinafter referred to as MS medium)
Dilute the medium (referred to as medium) to 1.2 times or more, and seal
! A method of culturing in a medium containing 1.0 to 3.0% by weight of 1.0 to 3.0% by weight is particularly preferred.
第２図に示す様に、ＬＳまたはＭＳの固定人工培地を使
用した場合に比較して、シ＝Ｉ糖ｔ、Ｏ〜３．０重量％
含有する液体培地のＬＳまたはＭＳ培地の無機塩組成を
希釈していくと大幅に不定芽３の誘導、増殖が促進され
る。特に、培地濃度を２倍に希釈した場合が最も効果的
であり、約３０倍の不定芽増殖率が確保できる。このこ
とは植物の組織培養を行なう場合、その生長、分化、増
殖に最適な培地の浸透圧があることを示している。特に
、液体培養を行なう場合、培地の浸透圧を調整すること
は効果的であり、通常のＬＳ培地で培養した場合、はと
んど生長、増殖が進行せず最後には枯死してしまうが、
ＬＳ培地を２倍に希釈し、ショ糖を１．０重量％に調整
して培養して培養した場合平均で３０倍、最大４５倍に
増殖することが可能である。As shown in Figure 2, compared to the case of using fixed artificial medium of LS or MS, the
When the inorganic salt composition of the liquid medium LS or MS medium contained therein is diluted, the induction and proliferation of adventitious buds 3 is greatly promoted. In particular, it is most effective when the medium concentration is doubled, and an adventitious bud multiplication rate of about 30 times can be ensured. This indicates that when performing plant tissue culture, there is an optimal osmotic pressure of the medium for growth, differentiation, and proliferation. In particular, when performing liquid culture, it is effective to adjust the osmotic pressure of the medium, and when cultured in normal LS medium, most of the cases do not grow or multiply and eventually die. ,
When the LS medium is diluted 2 times and the sucrose is adjusted to 1.0% by weight and cultured, it is possible to multiply by an average of 30 times and a maximum of 45 times.
更に、液体培地で培養する場合は、アンモニウムイオン
（ＮＨ４゛）と硝酸イオン（Ｎｏ：ｌ−）の量を調整す
る必要があり、第３図に示す様にＮＨ４＋／ＮＯ，−の
比が大きいと水で膨張したシュート、水浸状のシュート
が多発し、効率的な不定芽の増殖が困難となる。また、
ＮＨ４−イオンが皆無の場合は、生長不良となるため、
Ｎ）１．＋／ＮＯ，−０モル比には最適値が存在し、そ
の範囲は０．１〜０．５である。つまり、ギヨウジヤニ
ンニクの茎頂を、ＬＳ培地またはＭＳ培地の無機塩類濃
度を１．２倍以上に希釈し、ショ糖濃度を１．０〜３．
０重量％とじ、更に、［１４＋／ＮＯ，−のモル比を０
．１〜０．５の範囲に修正した液体培地において培養す
ることにより不定芽を効率的に誘導、増殖することが可
能となる。Furthermore, when culturing in a liquid medium, it is necessary to adjust the amounts of ammonium ions (NH4) and nitrate ions (No:l-), and as shown in Figure 3, the ratio of NH4+/NO,- is large. Shoots that are swollen with water or waterlogged occur frequently, making it difficult to efficiently propagate adventitious buds. Also,
If there are no NH4- ions, growth will be poor, so
N)1. There is an optimum value for the +/NO, -0 molar ratio, which ranges from 0.1 to 0.5. That is, the shoot apex of Japanese garlic is diluted to 1.2 times or more the inorganic salt concentration of LS medium or MS medium, and the sucrose concentration is 1.0 to 3.
The molar ratio of [14+/NO,- was reduced to 0% by weight.
．． By culturing in a liquid medium corrected to a range of 1 to 0.5, it becomes possible to efficiently induce and proliferate adventitious buds.
不定芽誘導に使用しうる培地中の植物ホルモンについて
は、サイトカイニンとして６−ベンジルアデニン、カイ
ネチンを、オーキシンとして１−ナフタリン酢酸、イン
ドール−３−酢酸、β−インドール醋酸を単独あるいは
組合せて濃度１〜３ｐｐｎ＋範囲で添加することが最適
である。Regarding plant hormones in the medium that can be used for adventitious bud induction, 6-benzyladenine and kinetin are used as cytokinins, and 1-naphthalene acetic acid, indole-3-acetic acid, and β-indole acetic acid are used as auxins, singly or in combination, at concentrations of 1 to 1. It is optimal to add in the 3ppn+ range.
培養環境としては、温度２５±５℃２照明度３０００〜
１５０００ルクスで培養するのが好ましい。また液体培
養の場合、１００〜１８０ｒｐＩＩで振とう培養するか
ジャファーメンタ−で培養することにより不定芽３を誘
導する。The culture environment is a temperature of 25±5°C, an illumination level of 3000~
Preferably, the culture is carried out at 15,000 lux. In the case of liquid culture, adventitious buds 3 are induced by shaking culture at 100 to 180 rpII or culturing in a Jaffer mentor.
不定芽の増殖方法としては、例えば茎頂を前記方法によ
り、１〜２ケ月培養を行ない、２０〜４０本程度誘導さ
れた不定芽をクリーンベンチ内で１本づつ分割し、不定
芽誘導条件と同様の条件で培養する方法が挙げられる。As a method for propagating adventitious buds, for example, the shoot apex is cultured for 1 to 2 months using the method described above, and approximately 20 to 40 adventitious buds are induced, which are divided one by one in a clean bench and then adjusted to the adventitious bud induction conditions. Examples include a method of culturing under similar conditions.
前記方法により誘導され、必要により増殖させた不定芽
３は、個々に分割した後、培養することによって発根さ
せる。The adventitious buds 3 induced by the method described above and propagated if necessary are divided into individuals and then cultured to root.
発根には、ＬＳ培地またＭＳ培地を基本培地とし、植物
ホルモンとしてサイトカイニンであるカイネチン、オー
キシンである１−ナフタリン酢酸を単独あるいは組み合
せて０．１〜２．０ｐｐｍ添加した固定培地を使用し、
温度２５±５℃５照明度３０００〜１００００ルクスで
培養することが好ましい。For rooting, use LS medium or MS medium as the basic medium, and use a fixed medium to which 0.1 to 2.0 ppm of the cytokinin kinetin and auxin 1-naphthalene acetic acid are added alone or in combination as plant hormones,
It is preferable to culture at a temperature of 25±5° C. and an illumination intensity of 3,000 to 10,000 lux.
上記のような方法で、不定芽３を培養することにより発
根したシュート４を誘導し、ついでこのシュートの根部
にリン茎５を形成せしめる。By culturing the adventitious buds 3 using the method described above, a rooted shoot 4 is induced, and then a lint stem 5 is formed at the root of this shoot.
リン茎５の誘導は、発根したシュート４を明照明下、高
温にて培養することにより行なうことができる。The phosphorus stem 5 can be induced by culturing the rooted shoot 4 under bright lighting at high temperature.
この場合、照明度３０００〜１５０００ルクス、温度は
３０±５℃であるのが好ましく、無機塩類を含有するが
植物ホルモンを含有しない培地で行なうのが特に好まし
い。In this case, the illumination intensity is preferably 3,000 to 15,000 lux and the temperature is preferably 30±5° C., and it is particularly preferable to use a medium containing inorganic salts but not containing plant hormones.
第４図に示す様に、高温、明照明の条件下で培養するこ
とにより、リン茎の誘導は促進される。As shown in FIG. 4, the induction of phosphorus stems is promoted by culturing under conditions of high temperature and bright lighting.
このことはギヨウジヤニンニクは自然界では春〜夏期に
かけてリン茎の形成が行なわれるため、試験管内（ｉｎ
ｖｉｔｒｏ ）においても、この条件を与えることによ
りシュート根部にリン茎を誘導することが可能となるた
めと考えられる。This is because in nature, the formation of phosphorus stems takes place from spring to summer, so it is possible to
This is thought to be because, even in vitro), providing these conditions makes it possible to induce phosphorus stems to the shoot roots.
リン茎５の誘導方法の一例として、前記方法により誘導
したギヨウジヤニンニクの発根したシュート４をＬＳ培
地またはＭＳ培地を基本培地とする固定培地へ移植し、
温度３０±５℃９照明度１５０００ルクスで培養するこ
とにより、人工的に休眠状態へ誘導し、試験管内で発根
したシュート４よりリン茎５を誘導する方法が挙げられ
る。As an example of the method for inducing the phosphorus stems 5, the rooted shoots 4 of the garlic cloves induced by the method described above are transplanted to a fixed medium with an LS medium or an MS medium as the basic medium,
An example of this method is to artificially induce a dormant state by culturing at a temperature of 30±5° C. and a lighting intensity of 15,000 lux, and to induce phosphorus stems 5 from shoots 4 that have taken root in a test tube.
以上、詳述したリン茎の増殖方法によって得られたリン
茎は、温度１〜５℃の低温で約２００日間長期保存が可
能である。The lin stems obtained by the above-described method for propagating lin stems can be stored for a long period of time at a low temperature of 1 to 5° C. for about 200 days.
また、そのリン茎を例えばＢＡを１１００ｐｐ含有する
水溶液で２４時間室温で処理することにより休眠を打破
した後、圃場で栽培することにより、ギヨウジヤニンニ
クの種苗として利用することが可能となる。In addition, by treating the phosphor stalks with an aqueous solution containing 1100 pp of BA at room temperature for 24 hours to break dormancy, and then cultivating them in the field, it becomes possible to use them as seedlings of Giyoja garlic.
ギヨウジヤニンニクの種苗大量増殖の実施例を以下に述
べる。An example of mass propagation of seeds and seedlings of Giyoja garlic will be described below.
ギヨウジヤニンニクリン茎を０．５％次亜鉛素酸ナトリ
ウム水溶液で滅菌後、滅菌水で充分洗浄してから、クリ
ーンベンチ内で大きさ２００〜５００μの茎頂を採取し
、それをベンジルアデニン（以下ＢＡと記す）１．０〜
３．０ｐｐｍ、ナフタリン酢酸（以下ＮＡＡと記す）１
．０〜３．Ｏｐｐ蒙、シｇＩ！１％、寒天０．７％含有
する２倍に希釈したＬＳ培地を基本培地とする固定培地
に置床し、温度２５±５℃、照度５０００ルクスで６０
日間培養すると不定芽が誘導される。After sterilizing the stems of Garlic Phosphorus with a 0.5% aqueous solution of sodium subzinc chlorate and thoroughly washing them with sterile water, the shoot tips with a size of 200 to 500 μm were collected in a clean bench, and they were treated with benzyladenine ( (hereinafter referred to as BA) 1.0~
3.0 ppm, naphthalene acetic acid (hereinafter referred to as NAA) 1
．． 0-3. Opp Meng, SigI! The base medium was a 2-fold diluted LS medium containing 1% agar and 0.7% agar.
Adventitious buds are induced when cultured for one day.
この不定芽を個々に分割し、上記同様の条件で培養する
ことにより増殖することが可能である。It is possible to propagate these adventitious buds by dividing them into individual buds and culturing them under the same conditions as above.
大規模に不定芽の誘導増殖を行なう場合、植物生長ホル
モンとしてＢＡを１．０〜２．０ｐｐｍ 、 ＮＡＡを
１．０〜２．０ｐｐｍ、及びシ＊Ｉ！１．０％を含有し
、ＮＨ，”／Ｎｏ、−０モル比を０．１〜０，５に修正
し、２倍に希釈したＬＳ液体培地を使用する。When performing induced multiplication of adventitious buds on a large scale, 1.0 to 2.0 ppm of BA, 1.0 to 2.0 ppm of NAA, and C*I! are used as plant growth hormones. A 2-fold diluted LS liquid medium containing 1.0% with a modified NH,''/No,-0 molar ratio of 0.1 to 0,5 is used.
この液体培地を１．５１程度、２． ＯＩｌ程度のエア
リストタイプのジャーファーメンタ−に入れ、茎頂２を
約１個／ ２５ ｃｃの密度で仕込み、空気通気量２０
０〜４００１ ／ ｍｉｎ、 温度２０’Ｃ，照度ａ
ｏｏｏルクスの条件で６０日間培養すると不定芽が誘導
される。About 1.51% of this liquid medium, 2. Place in an airist-type jar fermenter with about 100 ml of oil, fill with stem tips 2 at a density of about 1 piece/25 cc, and set the air aeration amount to 20.
0~4001/min, temperature 20'C, illuminance a
Adventitious buds are induced when cultured for 60 days under ooo lux conditions.
この不定芽を個々に分割し、上記同様の条件で培養する
ことにより増殖することが可能である。It is possible to propagate these adventitious buds by dividing them into individual buds and culturing them under the same conditions as above.
前記いずれかの方法で誘導した不定芽を個々に分割し、
ＬＳ培地を基本培地とし、植物生長ホルモンとしてカイ
ネチン０．１〜１．０ｐｐｍ、 ＮＡＡＯ，５〜１．
０ｐｐｍ、 ：／ −ｓ ｔ！ ３． Ｏｍ１％ヲ含有
ｔ ル固定培地へ移植する。温度２５±５℃１照度３０
００ルクスで３０日間培養し発根したシュートを形成さ
せる。Adventitious buds induced by any of the above methods are individually divided,
LS medium was used as the basic medium, and kinetin 0.1 to 1.0 ppm and NAAO, 5 to 1.0 ppm as plant growth hormones.
0ppm, :/-st! 3. Transfer to a fixed medium containing 1% Omol. Temperature 25±5℃ 1 Illuminance 30
Cultured at 00 lux for 30 days to form rooted shoots.
これを植物生長ホルモンを含有しないＬＳ培地を基本培
地とする固定培地へ移植し、温度３０±５℃、照度１０
０００ルクスで６０日間培養することにより発根したシ
ュートよりリン茎を誘導する。This was transferred to a fixed medium with LS medium that does not contain plant growth hormone as the basic medium, and the temperature was 30±5℃ and the illuminance was 10.
By culturing at 000 lux for 60 days, phosphorus stems are induced from the rooted shoots.
このリン茎は、温度１〜５℃の低温で約２００日間保存
することが可能である。This lin stem can be stored at a low temperature of 1 to 5°C for about 200 days.
このリン茎を圃場にて栽培する場合、ＢＡを１１００ｐ
ｐ含有する水溶液で２４時間処理し、休眠を打破した後
、圃場に播きギヨウジヤニンニク種苗とすることができ
る。When cultivating this phosphorus stem in the field, add 1100p of BA.
After breaking dormancy by treating with an aqueous solution containing P for 24 hours, the seeds can be sown in a field to produce Garlic seedlings.
本発明によれば、ギヨウジヤニンニクの栽培において、
大量の種苗を効率的に確保することが可能であり、これ
によって資源の枯渇を防げるばかりでなく、植物組織培
養により均一で優良な種苗を生産することが可能である
。According to the present invention, in the cultivation of Giyoja garlic,
It is possible to efficiently secure a large amount of seeds and seedlings, which not only prevents resource depletion but also makes it possible to produce uniform and high quality seeds and seedlings through plant tissue culture.
また、種子繁殖方法に比較して、種苗生産の期間を１７
３程度に短縮することが可能であり、増殖率も種子繁殖
が１親株あたり５倍／３年であるのに対して、本方法は
、１１を株あたり、３０倍／２ケ月であることから本方
法は、種子繁殖方法に比較して年間１００倍以上の種苗
生産能力の向上が可能となる。In addition, compared to the seed propagation method, the period of seedling production is 17%.
The multiplication rate can be reduced to about 3, and the multiplication rate is 5 times per parent plant/3 years for seed propagation, whereas this method is 30 times/2 months per 11 plants. This method can improve seedling production capacity by more than 100 times per year compared to seed propagation methods.
第１図はギヨウジヤニンニク種苗生産システム概略図、
第２図は基本培地希釈の効果を示すグラフ、第３図はＮ
）１．’／ＮＯ３−モル比と水浸状面の発生率の関係を
示すグラフ、第４図は培養温度、照度とリン茎誘導率の
関係を示すグラフである。Figure 1 is a schematic diagram of the Giyojia garlic seedling production system.
Figure 2 is a graph showing the effect of basic medium dilution, Figure 3 is N
)1. Figure 4 is a graph showing the relationship between the '/NO3 molar ratio and the incidence of water-soaked surfaces, and FIG.
Claims (1)
不定芽を誘導し、必要に応じて前記不定芽を増殖させた
後、該不定芽を個々に分割し、培養して発根したシュー
トを得、ついでこのシュートの根部にリン茎を形成せし
めることを特徴とするギョウジャニンニクのリン茎の大
量増殖方法。 ２、外植片が茎頂であることを特徴とする請求項１記載
のギョウジャニンニクのリン茎の大量増殖法。 ３、ギョウジャニンニクの茎頂を、リンスマイヤー−ス
クーグ培地またはムラシゲ−スクーグ培地の無機塩類濃
度を１．２倍以上に希釈した無機塩類組成を有し、かつ
、ショ糖を１．０〜３．０重量％の濃度で含有する培地
において培養することにより前記茎頂より不定芽を誘導
することを特徴とするギョウジャニンニクの不定芽誘導
方法。 ４、ギョウジャニンニクの茎頂を、リンスマイヤー−ス
クーグ培地またはムラシゲ−スクーグ培地の無機塩類濃
度を１．２倍以上に希釈した無機塩類組成を有し、ショ
糖を１．０〜３０．重量％の濃度で含有し、かつＮＨ＿
４＾＋／ＮＯ＿３＾−のモル比を０．１〜０．５の範囲
に修正した液体培地において培養することにより前記茎
頂より不定芽を誘導することを特徴とするギョウジャニ
ンニクの不定芽誘導方法。 ５、ギョウジャニンニクにおいて、外植片より誘導した
シュートを照明下、高温にて培養を行なうことにより、
前記シュートの根部にリン茎を誘導することを特徴とす
るギョウジャニンニクのリン茎の誘導方法。 ６、高温が３０±５℃であることを特徴とする請求項５
記載のギョウジャニンニクのリン茎の誘導方法。 ７、照明度が３０００〜１５０００ルクスであることを
特徴とする請求項５又は６記載のギョウジャニンニクの
リン茎の誘導方法。 ８、請求項１〜３のいずれかの項記載の大量増殖方法に
よって得られたリン茎を、休眠を打破し、さらに馴化さ
せ種苗とすることを特徴とするギョウジャニンニクの種
苗大量増殖方法。[Scope of Claims] 1. After inducing adventitious buds by culturing explants of Gyoja garlic, and multiplying the adventitious buds as necessary, the adventitious buds are divided into individual parts and cultured to allow them to develop. A method for mass propagating phosphorus stems of Gyoja garlic, which is characterized by obtaining rooted shoots and then forming phosphorus stems at the roots of the shoots. 2. The method for mass propagation of phosphorus stems of Gyoja garlic according to claim 1, wherein the explant is a shoot apex. 3. The shoot tip of Gyoja garlic has an inorganic salt composition diluted to 1.2 times or more the inorganic salt concentration of Linsmeyer-Skoog medium or Murashige-Skoog medium, and sucrose is 1.0 to 3.0. 1. A method for inducing adventitious buds of Gyoja garlic, which comprises inducing adventitious buds from the shoot apex by culturing in a medium containing a concentration of % by weight. 4. The shoot apex of Gyoja garlic has an inorganic salt composition diluted to 1.2 times or more the inorganic salt concentration of Linsmeyer-Skoog medium or Murashige-Skoog medium, and sucrose is 1.0 to 30. Contains at a concentration of % by weight, and NH_
A method for inducing adventitious buds of Gyoja garlic, which comprises inducing adventitious buds from the shoot apex by culturing in a liquid medium in which the molar ratio of 4^+/NO_3^- is adjusted to a range of 0.1 to 0.5. . 5. In Gyoja garlic, by culturing shoots induced from explants under lighting at high temperature,
A method for inducing phosphorus stems in Gyoja garlic, the method comprising inducing phosphorus stems to the roots of the shoots. 6. Claim 5 characterized in that the high temperature is 30±5°C.
The described method for inducing lin stems of Gyoja garlic. 7. The method for inducing phosphorus stems of Japanese garlic according to claim 5 or 6, characterized in that the illumination intensity is 3,000 to 15,000 lux. 8. A method for mass propagating seeds and seedlings of Gyoja garlic, which comprises breaking the dormancy of the phosphorus stems obtained by the method for mass propagating according to any one of claims 1 to 3, and further acclimatizing them to use them as seeds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1107217A JPH02286020A (en) | 1989-04-28 | 1989-04-28 | Massive multiplication of allium victorialis l. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1107217A JPH02286020A (en) | 1989-04-28 | 1989-04-28 | Massive multiplication of allium victorialis l. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH02286020A true JPH02286020A (en) | 1990-11-26 |
Family
ID=14453457
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1107217A Pending JPH02286020A (en) | 1989-04-28 | 1989-04-28 | Massive multiplication of allium victorialis l. |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH02286020A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011172547A (en) * | 2010-02-25 | 2011-09-08 | Aomori Prefectural Industrial Technology Research Center | Method for promoting blooming of plants of allium genus, self-propagating seeds, cross-breeding seeds, self-propagating method, mating method, method for creating virus-free individual, and virus-free individual |
| CN105265316A (en) * | 2015-11-02 | 2016-01-27 | 吉林省蔬菜花卉科学研究院 | Rapid propagation method for plateaus of alliums |
| CN105265317A (en) * | 2015-11-02 | 2016-01-27 | 吉林省蔬菜花卉科学研究院 | Rapid propagation method of allium victorialis |
| CN111492981A (en) * | 2020-06-11 | 2020-08-07 | 包头市农牧业科学研究院 | Tissue culture rapid propagation method of red onions |
-
1989
- 1989-04-28 JP JP1107217A patent/JPH02286020A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011172547A (en) * | 2010-02-25 | 2011-09-08 | Aomori Prefectural Industrial Technology Research Center | Method for promoting blooming of plants of allium genus, self-propagating seeds, cross-breeding seeds, self-propagating method, mating method, method for creating virus-free individual, and virus-free individual |
| CN105265316A (en) * | 2015-11-02 | 2016-01-27 | 吉林省蔬菜花卉科学研究院 | Rapid propagation method for plateaus of alliums |
| CN105265317A (en) * | 2015-11-02 | 2016-01-27 | 吉林省蔬菜花卉科学研究院 | Rapid propagation method of allium victorialis |
| CN111492981A (en) * | 2020-06-11 | 2020-08-07 | 包头市农牧业科学研究院 | Tissue culture rapid propagation method of red onions |
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