JPS6398318A - Culture of raw log of edible mushrooms - Google Patents

Culture of raw log of edible mushrooms

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Publication number
JPS6398318A
JPS6398318A JP61243024A JP24302486A JPS6398318A JP S6398318 A JPS6398318 A JP S6398318A JP 61243024 A JP61243024 A JP 61243024A JP 24302486 A JP24302486 A JP 24302486A JP S6398318 A JPS6398318 A JP S6398318A
Authority
JP
Japan
Prior art keywords
water
culture
ceramics
treated water
logs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP61243024A
Other languages
Japanese (ja)
Inventor
昌夫 森
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DAICHI BUSSAN KK
Original Assignee
DAICHI BUSSAN KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DAICHI BUSSAN KK filed Critical DAICHI BUSSAN KK
Priority to JP61243024A priority Critical patent/JPS6398318A/en
Publication of JPS6398318A publication Critical patent/JPS6398318A/en
Pending legal-status Critical Current

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  • Mushroom Cultivation (AREA)

Abstract

(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。
(57) [Abstract] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

【発明の詳細な説明】 (A)発明の目的について (イ)産業上の利用分野 本発明はシイタケ、ナメコ、シメシ等原木栽培をなす食
用茸類の栽培方法の改善に関するものである。
DETAILED DESCRIPTION OF THE INVENTION (A) Object of the Invention (a) Field of Industrial Application The present invention relates to an improvement in the method for cultivating edible mushrooms such as shiitake, nameko, and shimeko, which are grown on logs.

(ロ)従来の技術について この種食用茸類の原木栽培は、普通精水を使用した寒天
培地を殺菌処理(普通の水を使用した高圧釜を使用。)
しこれに完熟茸の切片を置いて寒天培養をなし、更に殺
菌処理(普通の水を使用した高圧釜を使用。)した拡大
培地で拡大培養して母菌を生成し、この母菌を原木に植
苗の後仮伏(初期培養)して伏込み(後期培養)を行い
、次いで通常の水に充分浸漬して本伏せを行うという技
術手段を採用していた。又母菌は菌種駒に培養しこれを
原木に打ち込むという技術手段も一般的に採用されてい
る。
(b) Regarding conventional technology Log cultivation of this type of edible mushroom involves sterilizing an agar medium using ordinary purified water (using a high-pressure pot using ordinary water).
Then, a section of a ripe mushroom is placed on top of this and cultured on agar, which is then expanded and cultured in an expansion medium that has been sterilized (using a high-pressure pot using ordinary water) to produce mother bacteria. After seedlings are planted, they are suspended (initial culture) and then submerged (late culturing), and then the seedlings are fully soaked in regular water for final submergence. In addition, a technical method is generally adopted in which the mother fungus is cultured on a seed piece and then driven into logs.

(ハ)本発明が解決しようとする問題点前記のような従
来の栽培方法では、茸菌の寒天培養及び拡大培養におい
ては雑菌発生率が高く従って培養菌の発菌率及び活着率
が70%〜80%と低いという難点があった。又植菌本
伝後茸発生迄2年という歳月を要し、一度収穫してから
次の発生収穫には一カ月以上原木を休養させなければな
らないという時間的ロスと、原木の生産寿命が4年〜5
年と短く且つ年々発生する茸の量及び質が低下するとい
う難点があると共に原木の不足と高騰という問題点が存
在した。
(c) Problems to be solved by the present invention In the conventional cultivation methods as described above, the incidence of various bacteria is high in agar culture and expansion culture of mushroom fungi, and the germination rate and survival rate of cultured bacteria are 70%. The problem was that it was as low as ~80%. In addition, it takes two years for mushrooms to emerge after inoculation, and there is a time loss in that the logs must be allowed to rest for more than a month after they are harvested, and the production life of the logs is limited. 4th to 5th year
There is a problem that the number of years is short and the quantity and quality of mushrooms that are produced decreases year by year, as well as a shortage of logs and rising prices.

そこで本発明は以上に鑑み食茸菌の培養時において雑菌
の発生率を少くして且つ発菌率、活着率の大なる菌を培
養すると共に、生産サイクルの短縮及び原木の生産寿命
(年数)の長期化並びに収穫量の増収を図り生産性の向
」二を目的としたものである。
Therefore, in view of the above, the present invention reduces the incidence of miscellaneous bacteria when culturing edible mushroom fungi, cultivates bacteria with a high germination rate and survival rate, shortens the production cycle, and reduces the production life (years) of logs. The purpose of this is to increase productivity by prolonging the harvest and increasing yield.

(B)発明の構成について 本発明は以上の目的をもってなされたものであるが、次
にその構成について図面に従って説明する。第1図は組
織培養及び拡大培養の作用説明図であるが、寒天及び澱
粉、ビタミン等栄養素を、セラミックスに流液又は当接
して得たセラミックス処理水(2)で混合溶解し、これ
を試験管(6)に入れてセラミックス処理水(2)使用
の殺菌釜(3)で蒸気殺菌して寒天培地(1)となし、
この寒天培地(1)に食用茸から切り取った食用茸組織
(4)を植え培養する。なお殺菌釜(3)には水とセラ
ミックス(17)を入れてセラミックス処理水(2)と
するのが便利である。
(B) Regarding the structure of the invention The present invention has been made with the above-mentioned objectives. Next, the structure will be explained with reference to the drawings. Figure 1 is an explanatory diagram of the effects of tissue culture and expansion culture. Nutrients such as agar, starch, and vitamins are mixed and dissolved in ceramic-treated water (2) obtained by flowing or contacting ceramics, and this is tested. Put it in a tube (6) and steam sterilize it in a sterilization pot (3) using ceramic treated water (2) to make an agar medium (1).
Edible mushroom tissues (4) cut from edible mushrooms are planted and cultured on this agar medium (1). It is convenient to put water and ceramics (17) into the sterilization pot (3) to obtain ceramics-treated water (2).

他方、オガクズその他の栄養素からなる拡大培地材料(
5)をセラミックス処理水(2)でよく混合し全体の含
水量を55%〜65%程度とする。この拡大培地材料(
5)をセラミックス処理水(2)を使用した殺菌釜(3
′)で殺菌して拡大培地(7)となし、この拡大培地(
7)に前記組織培養の茸菌(4′)を入れて培養室(1
4)で拡大培養して母菌(8)を成牛ずる。
On the other hand, expansion medium materials consisting of sawdust and other nutrients (
5) is thoroughly mixed with ceramic treated water (2) to bring the total water content to about 55% to 65%. This expansion medium material (
5) in a sterilization pot (3) using ceramic-treated water (2).
’) to form an expansion medium (7), and this expansion medium (
7), put the tissue cultured mushroom fungi (4') into the culture chamber (1).
Expand the culture in step 4) and remove the mother bacteria (8) from adult cows.

この際培養室(14)の湿度を60%〜70%とするの
が最適であるが乾燥するようなときはセラミックス処理
水(2)を適宜撒水する。
At this time, it is optimal to keep the humidity in the culture chamber (14) at 60% to 70%, but if the culture room (14) becomes dry, sprinkle ceramics-treated water (2) as appropriate.

次に第2図は植苗及び原木培養の作用説明図であるが、
上記のようにして拡大培養した母菌(8)を原木(9)
に植え込み封臘(13)して被覆物(15)で覆いをな
し仮状培養(lO)をし、次いで井桁に組んで伏込培養
(11)を行い、更に、浸漬槽(16)でセラミックス
処理水(2)に充分浸漬する。図中(18)はセラミッ
クス水処理管でこの中にセラミックス(17)を入れて
ホース(19)で通水し水をセラミックス(17)に流
液せしめてセラミックス処理水(2)となし伏込培養(
11)した原木(9)を浸漬処理している。この際セラ
ミックス処理水(2)は浸漬槽(16)内を常に環流し
且つ一方から流出するように構成する。又この浸漬処理
は浸漬槽(16)に限定されるものではなく浸漬池でも
よいし、又必要とするだけ原木(9)にセラミックス処
理水(2)を撒水してもよい。
Next, Figure 2 is an explanatory diagram of the effects of planting seedlings and log cultivation.
The mother bacteria (8) expanded as described above are grown on logs (9).
The ceramics were implanted in a container and sealed (13), covered with a coating material (15), and subjected to pseudo-culture (1O), then placed in a well and subjected to submerged cultivation (11), and then placed in a dipping tank (16). Thoroughly immerse in treated water (2). In the figure, (18) is a ceramic water treatment pipe.Ceramics (17) is placed in the pipe, water is passed through it with a hose (19), and the water is allowed to flow through the ceramics (17), which is then turned into ceramic-treated water (2). culture(
11) The raw wood (9) is soaked. At this time, the ceramics treated water (2) is configured to constantly circulate inside the immersion tank (16) and flow out from one side. Further, this immersion treatment is not limited to the immersion tank (16), but may be performed in a immersion pond, or the ceramic treated water (2) may be sprinkled on the raw wood (9) as much as necessary.

このようにしてセラミックス処理水(2)に充分浸漬し
た原木(9)を本伝(12)をなし第一回目の茸発生収
穫後は、原木(9)(即ち原木に満延している菌糸。)
の疲労回復措置期間を設けないで直ちに原木(9)をセ
ラミックス処理水(2)に浸漬処理して本伝(12)を
なし収穫を繰返す。本発明は以上のような構成からなっ
ているが、なお、組織培養、拡大培養に使用するセラミ
ックス処理水(2)はセラミックス水処理管(18)内
を通水して得てもよいし、器に水とセラミックス(17
)を入れて処理水としてもよいし、セラミックス(17
)に水を当接、流液などいずれの手段を用いてもよい。
The log (9) sufficiently immersed in the ceramics-treated water (2) in this way is subjected to the main process (12), and after the first mushroom generation is harvested, the log (9) (i.e., the mycelia that are fully spread on the log) .)
Immediately, the raw wood (9) is immersed in ceramic treatment water (2) without any fatigue recovery period, and the main process (12) is carried out and harvesting is repeated. Although the present invention has the above-described configuration, the ceramic treated water (2) used for tissue culture and expansion culture may be obtained by passing water through the ceramic water treatment pipe (18), Water and ceramics in a container (17
) can be used as treated water, or ceramics (17
) may be used by any means, such as applying water to the surface or flowing liquid.

(C)発明の作用及び効果について 本発明は以上のような構成を有するものであるが、次に
その作用及び効果について述べる寒天培地(1)は寒天
及び澱粉、ビタミン等々の栄養素(2′)からなり、こ
れらの寒天培地材料(2′)をセラミックス(17)に
流液又は当接して得たセラミックス処理水(2)で溶解
してこれを試験管(6)に入れてセラミックス処理水(
2)を使用した蒸気式の殺菌釜(3)で一時間蒸気殺菌
して寒天培地(1)となしこの寒天培地(1)の上に食
用茸組織(4)を乗せ培養室(20)に入れて食用茸菌
の組織培養を行う。培養室(20)の温度及び湿度並び
に−酸化倹素濃度は従来の培養手段と同様であるが、本
発明のようにセラミックス処理水(2)を使用した寒天
培地(1)を使用した場合は、セラミックス処理水を使
用しない従来の手段に比して次の表に示すような大きな
効果がある。
(C) Functions and Effects of the Invention The present invention has the above structure, but the agar medium (1), whose functions and effects will be described next, contains agar and nutrients such as starch and vitamins (2'). The agar medium material (2') is dissolved in the ceramics-treated water (2) obtained by flowing or contacting the ceramics (17), and the solution is placed in a test tube (6) to dissolve the ceramics-treated water (2').
Steam sterilize the mixture for one hour in a steam sterilization pot (3) using 2) to form an agar medium (1). Place the edible mushroom tissue (4) on top of this agar medium (1) and place it in the culture chamber (20). to perform tissue culture of edible mushroom fungi. The temperature and humidity of the culture chamber (20) and the concentration of oxidized chlorine are the same as those of conventional culture means, but when using the agar medium (1) using ceramic-treated water (2) as in the present invention, Compared to conventional means that do not use ceramic-treated water, this method has significant effects as shown in the following table.

」二記の表に示すようにセラミックス処理水を使用した
場合は雑菌率が激しく低下し従って発菌率が向」−する
と共に菌の生育も従来方法に比して3日乃至4日翳いと
いう作用効果が得られる。即ち活性力ある食用茸菌(4
′)が得られる。
``As shown in the table below, when ceramic-treated water is used, the rate of bacteria is drastically reduced, and the germination rate is therefore improved.'' At the same time, the growth of bacteria is also delayed for 3 to 4 days compared to the conventional method. This effect can be obtained. In other words, active edible mushroom fungi (4
′) is obtained.

次に、拡大培地材料(5)をセラミックス処理水(2)
で混合処理すると共にセラミックス処理水(2)で蒸気
殺菌(殺菌釜使用)して拡大培地(7)を製し、この拡
大培地(7)で前記の寒天培養した食用茸菌(4′)を
培養室(]4)で拡大培養するのであるが、培養中、培
養室(14)内の温度、湿度、−酸化炭素濃度は従来の
手段と変りはないが、湿度保持のため撒水するときはセ
ラミックス処理水(2)を使用する。然るときは2日乃
至3日で発菌するが、セラミックス処理水を使用しない
従来技術手段と比較すると次の表に示す通り効果に差が
生ずる。
Next, the expansion medium material (5) is added to the ceramic treated water (2).
and steam sterilization (using a sterilization pot) with ceramic-treated water (2) to prepare an expansion medium (7), in which the agar-cultured edible mushroom fungus (4') is grown. The culture is expanded in the culture room (4), and during culture, the temperature, humidity, and carbon oxide concentration in the culture room (14) are the same as in conventional methods, but when watering to maintain humidity, Use ceramic treated water (2). In such cases, germs will develop in 2 to 3 days, but when compared with conventional techniques that do not use ceramic-treated water, there is a difference in effectiveness as shown in the following table.

上記のようにセラミックス処理水(2)を使用するとき
は雑菌率が低下し、発菌率、活着率が向上し菌勢の極め
て良好な母菌(8)を得ることができる。
When the ceramic-treated water (2) is used as described above, the rate of miscellaneous bacteria is reduced, the germination rate and survival rate are improved, and it is possible to obtain mother bacteria (8) with extremely good bacterial strength.

次に、以上のようにして培養した母菌(8)を原木(9
)に植菌する。植菌手段は従来のそれと変りはないが植
菌する際原木(9)にセラミックス処理水(2)を撒水
することが望ましい。然し植菌、仮伏、本状後セラミッ
クス処理水(2)に浸漬するのでこれを省略してもよい
。植苗は原木(9)に適宜穴をあけ母菌(8)を植え封
臘(13)する。植苗後の原木培養手段である仮状培養
(10)及び伏込培養(11)手段は従来の手段と同様
である。図中(15)は仮状培養(10)中の被覆物で
ある。
Next, the mother fungus (8) cultured as described above was added to the log (9).
). The inoculation method is the same as the conventional one, but it is desirable to sprinkle the ceramic treated water (2) on the raw wood (9) when inoculating the inoculation. However, since the ceramics are immersed in the ceramic treatment water (2) after inoculation, temporary incubation, and main formation, this step may be omitted. For planting seedlings, make appropriate holes in logs (9), plant mother fungi (8), and seal (13). The pseudo culture (10) and submerged culture (11) means for culturing logs after planting seedlings are the same as conventional methods. In the figure (15) is the coating in the pseudoculture (10).

次に伏込培養(11)が終了したならば原木(9)を水
中に浸漬処理するのであるが原木(9)を浸漬槽(16
)に入れ、当該槽(16)内でセラミックス処理水(2
)を環流更新しながら原木(9)に充分に含湿せしめる
。この際セラミックス処理水(2)はセラミックス水処
理管(18)にセラミックス(17)を内蔵し、これに
ホース(19)で通水してセラミックス処理水(2)と
なし浸漬槽(16)内を環流させながら浸漬槽(16)
上部の排水口(21)から排水し、浸漬槽(16)内で
は常に新しいセラミックス処理水(2)が環流するよう
にしである。なお浸漬槽に限らず浸漬池を使用する場合
においても同様とする。このようにして植菌した原木(
9)に充分セラミックス処理水(2)を滲潤せしめ(通
常では24時間で充分である。)、次いで浸漬槽(16
)から原木(9)を取り出して本状(12)する。然る
ときは、実験の結果 (A)従来方法では菌の活着率が上71<80%程度で
あったが、本発明では」00%の活着率である。
Next, when the submerged culture (11) is completed, the log (9) is immersed in water.
), and in the tank (16), the ceramics treated water (2
) is circulated and renewed to sufficiently moisten the log (9). At this time, the ceramics-treated water (2) contains ceramics (17) in the ceramics water treatment pipe (18), and water is passed through it with a hose (19) to form the ceramics-treated water (2) inside the dipping tank (16). Immersion tank (16) while circulating
Drainage is carried out through the drain port (21) at the top, and fresh ceramics-treated water (2) always circulates in the immersion tank (16). The same applies not only to immersion tanks but also to immersion ponds. Logs inoculated in this way (
9) is sufficiently soaked with the ceramic treatment water (2) (usually 24 hours is sufficient), and then soaked in the immersion tank (16
) is taken out from the log (9) and made into a book (12). In such cases, experimental results (A) showed that the conventional method had a bacterial survival rate of about 71<80%, but the present invention had a survival rate of 0.00%.

(B)従来方法では原木(9)の雑菌発生率が10%〜
15%であったが、本発明では2%程度である。
(B) In the conventional method, the incidence of bacteria on log (9) is 10% ~
Although it was 15%, it is about 2% in the present invention.

(C)従来方法では本状(12)から茸発生まで最低2
年を要していたが、本発明では1年で茸の発生を見る。
(C) In the conventional method, it takes at least 2 times from the main state (12) to the appearance of mushrooms.
It used to take many years, but with the present invention, mushrooms can be grown in one year.

(D)従来方法では、一度収穫したならば原木(9)を
必ず1ケ月間休ませて菌の疲労を回復してから再び水に
浸漬して本状(12)をして茸の発生を待つが、本発明
の場合は収穫接菌の疲労回復期間を置かないで、直ちに
浸漬槽(16)(又は浸漬池)で原木(9)をセラミッ
クス処理水(2)に浸漬して本状(12)をなし翌年に
は茸の発生収穫が可能である。
(D) In the conventional method, once harvested, the log (9) must be rested for one month to recover bacterial fatigue, and then immersed in water again to form the main form (12) to prevent the growth of mushrooms. However, in the case of the present invention, the raw wood (9) is immediately immersed in the ceramics-treated water (2) in the soaking tank (16) (or soaking pond) without allowing a fatigue recovery period after harvest inoculation. 12), and the mushrooms can be harvested the following year.

(E)従来の方法では原木(9)の寿命は4年〜5年で
、然も3年目からは茸の品質が低下し、その後は茸芽の
発生は見るが成茸には生長しないが、本発明による場合
は8年〜9年間品質良好な茸を収穫することが可能であ
ることが確められた。
(E) With the conventional method, the lifespan of the log (9) is 4 to 5 years, but the quality of the mushrooms deteriorates from the third year onward, and after that, mushroom buds appear but do not grow into adult mushrooms. However, it has been confirmed that according to the present invention, it is possible to harvest mushrooms of good quality for 8 to 9 years.

(F)以上のような大きな効用が存在するので毎年の収
穫量は従来の方法に比較して30%以上の増収となるば
かりでなく原木(9)の寿命が倍も長くなったので同一
原木(9)で全収穫量は少くとも3倍以上となり生産性
が極めて高く原木不足にも対応可能であるという極めて
大きな利点を有するものである。
(F) Because of the great benefits mentioned above, not only does the annual yield increase by more than 30% compared to the conventional method, but the lifespan of logs (9) has also doubled, so even if the same log In (9), the total yield is at least tripled, which is extremely high productivity, and it has the extremely great advantage of being able to cope with a shortage of logs.

【図面の簡単な説明】[Brief explanation of the drawing]

図面は実施例を示す作用説明図で、 第1図は食用茸菌の組織培養及び拡大培養図であり、 第2図は植苗及び原木培養並びに本伝図である。 (1)・・・・・・・寒天培地 (16)旧・・・・・
原木の浸漬槽(2)・・・・・・・・セラミックス処理
水(3)・・・・・・殺菌釜  (17) 叫−、、、
セラミックス(4)・・ ・・・食用茸組織(18)・
・叩・・・セラミックス(7)・・・・・・・拡大培地
      水処理管(8)・・・・・・母菌
The drawings are action explanatory diagrams showing examples. Fig. 1 is a diagram of tissue culture and expanded culture of edible mushroom fungi, and Fig. 2 is a diagram of planted seedlings, log culture, and this biography. (1)・・・・・・Agar medium (16) Old・・・・・・
Log soaking tank (2) Ceramics treated water (3) Sterilization pot (17)
Ceramics (4)... Edible mushroom tissue (18)...
- Beating...Ceramics (7)...Expansion medium Water treatment tube (8)...Mother bacteria

Claims (1)

【特許請求の範囲】[Claims] 食用茸類の菌組織培養及びその拡大培養において、寒天
培地(1)及び拡大培地(7)をつくる場合各培地材料
(2′)(5)の混合及び殺菌処理に使用する水はすべ
てセラミックス処理水(2)を使用して母菌(8)を生
成し、又原木(9)に母菌(8)を植菌し本伏(12)
に至るまでの原木培養においては、伏込培養(11)後
の原木水中浸漬処理にはセラミックス処理水(2)を使
用し、更に第一回収穫後は菌の疲労回復期間を設けず直
ちにセラミックス処理水(2)に浸漬処理して本伏(1
2)と収穫を繰返すことを特徴とする食用茸類の原木栽
培方法。
When making agar medium (1) and expansion medium (7) in bacterial tissue culture of edible mushrooms and its expansion culture, all water used for mixing and sterilization of each medium material (2') (5) is treated with ceramics. Use water (2) to generate mother fungi (8), and inoculate logs (9) with mother fungi (8) to produce honbushi (12).
In the cultivation of logs up to the stage of culturing, ceramics-treated water (2) is used to immerse the logs in water after submergence cultivation (11), and after the first harvest, ceramics are immediately grown without a period for the bacteria to recover from fatigue. Honbushi (1) is immersed in treated water (2).
2) A method for cultivating logs of edible mushrooms characterized by repeated harvesting.
JP61243024A 1986-10-15 1986-10-15 Culture of raw log of edible mushrooms Pending JPS6398318A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP61243024A JPS6398318A (en) 1986-10-15 1986-10-15 Culture of raw log of edible mushrooms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP61243024A JPS6398318A (en) 1986-10-15 1986-10-15 Culture of raw log of edible mushrooms

Publications (1)

Publication Number Publication Date
JPS6398318A true JPS6398318A (en) 1988-04-28

Family

ID=17097734

Family Applications (1)

Application Number Title Priority Date Filing Date
JP61243024A Pending JPS6398318A (en) 1986-10-15 1986-10-15 Culture of raw log of edible mushrooms

Country Status (1)

Country Link
JP (1) JPS6398318A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02104278A (en) * 1988-10-13 1990-04-17 Akira Yamazaki Method for culturing mycelia of basidiomycete or ascomycete

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02104278A (en) * 1988-10-13 1990-04-17 Akira Yamazaki Method for culturing mycelia of basidiomycete or ascomycete

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