JPS6352708B2 - - Google Patents
Info
- Publication number
- JPS6352708B2 JPS6352708B2 JP5277981A JP5277981A JPS6352708B2 JP S6352708 B2 JPS6352708 B2 JP S6352708B2 JP 5277981 A JP5277981 A JP 5277981A JP 5277981 A JP5277981 A JP 5277981A JP S6352708 B2 JPS6352708 B2 JP S6352708B2
- Authority
- JP
- Japan
- Prior art keywords
- latex
- antibodies
- antigens
- antibody
- antigen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000004816 latex Substances 0.000 claims description 91
- 229920000126 latex Polymers 0.000 claims description 91
- 239000000427 antigen Substances 0.000 claims description 47
- 102000036639 antigens Human genes 0.000 claims description 46
- 108091007433 antigens Proteins 0.000 claims description 46
- 239000003153 chemical reaction reagent Substances 0.000 claims description 45
- 238000006243 chemical reaction Methods 0.000 claims description 28
- 210000002966 serum Anatomy 0.000 claims description 28
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims description 23
- 230000004520 agglutination Effects 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 14
- 238000005259 measurement Methods 0.000 claims description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 239000003995 emulsifying agent Substances 0.000 claims description 9
- AGBXYHCHUYARJY-UHFFFAOYSA-N 2-phenylethenesulfonic acid Chemical compound OS(=O)(=O)C=CC1=CC=CC=C1 AGBXYHCHUYARJY-UHFFFAOYSA-N 0.000 claims description 8
- 239000003999 initiator Substances 0.000 claims description 7
- 230000003287 optical effect Effects 0.000 claims description 7
- JRKICGRDRMAZLK-UHFFFAOYSA-L peroxydisulfate Chemical compound [O-]S(=O)(=O)OOS([O-])(=O)=O JRKICGRDRMAZLK-UHFFFAOYSA-L 0.000 claims description 6
- 238000007334 copolymerization reaction Methods 0.000 claims description 5
- 239000002245 particle Substances 0.000 description 15
- 230000035945 sensitivity Effects 0.000 description 12
- 102000004169 proteins and genes Human genes 0.000 description 9
- 108090000623 proteins and genes Proteins 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- 241000700199 Cavia porcellus Species 0.000 description 7
- 150000001875 compounds Chemical class 0.000 description 7
- 239000008363 phosphate buffer Substances 0.000 description 7
- 108010088751 Albumins Proteins 0.000 description 6
- 102000009027 Albumins Human genes 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 206010029719 Nonspecific reaction Diseases 0.000 description 4
- 241000283973 Oryctolagus cuniculus Species 0.000 description 4
- 238000004220 aggregation Methods 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 238000000691 measurement method Methods 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 238000001042 affinity chromatography Methods 0.000 description 3
- 230000002776 aggregation Effects 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 230000006303 immediate early viral mRNA transcription Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000178 monomer Substances 0.000 description 3
- 239000008055 phosphate buffer solution Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 229910002012 Aerosil® Inorganic materials 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 241000700198 Cavia Species 0.000 description 2
- 241000700721 Hepatitis B virus Species 0.000 description 2
- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 description 2
- 239000003125 aqueous solvent Substances 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 230000000984 immunochemical effect Effects 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 2
- 230000001235 sensitizing effect Effects 0.000 description 2
- MNCGMVDMOKPCSQ-UHFFFAOYSA-M sodium;2-phenylethenesulfonate Chemical compound [Na+].[O-]S(=O)(=O)C=CC1=CC=CC=C1 MNCGMVDMOKPCSQ-UHFFFAOYSA-M 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- LCPVQAHEFVXVKT-UHFFFAOYSA-N 2-(2,4-difluorophenoxy)pyridin-3-amine Chemical compound NC1=CC=CN=C1OC1=CC=C(F)C=C1F LCPVQAHEFVXVKT-UHFFFAOYSA-N 0.000 description 1
- 239000004925 Acrylic resin Substances 0.000 description 1
- 229920000178 Acrylic resin Polymers 0.000 description 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- 238000011891 EIA kit Methods 0.000 description 1
- 108091006905 Human Serum Albumin Proteins 0.000 description 1
- 102000008100 Human Serum Albumin Human genes 0.000 description 1
- 108090000942 Lactalbumin Proteins 0.000 description 1
- 102000004407 Lactalbumin Human genes 0.000 description 1
- 241000283977 Oryctolagus Species 0.000 description 1
- 108010058846 Ovalbumin Proteins 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 102000013529 alpha-Fetoproteins Human genes 0.000 description 1
- 108010026331 alpha-Fetoproteins Proteins 0.000 description 1
- 229910001870 ammonium persulfate Inorganic materials 0.000 description 1
- XDIJWRHVEDUFGP-UHFFFAOYSA-N azanium;2-phenylethenesulfonate Chemical compound [NH4+].[O-]S(=O)(=O)C=CC1=CC=CC=C1 XDIJWRHVEDUFGP-UHFFFAOYSA-N 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 108010074605 gamma-Globulins Proteins 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 229940106780 human fibrinogen Drugs 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- ZKIAYSOOCAKOJR-UHFFFAOYSA-M lithium;2-phenylethenesulfonate Chemical compound [Li+].[O-]S(=O)(=O)C=CC1=CC=CC=C1 ZKIAYSOOCAKOJR-UHFFFAOYSA-M 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 229940092253 ovalbumin Drugs 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- QIFCIFLDTQJGHQ-UHFFFAOYSA-M potassium;2-phenylethenesulfonate Chemical compound [K+].[O-]S(=O)(=O)C=CC1=CC=CC=C1 QIFCIFLDTQJGHQ-UHFFFAOYSA-M 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical compound O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- KKCBUQHMOMHUOY-UHFFFAOYSA-N sodium oxide Chemical compound [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 description 1
- 229910001948 sodium oxide Inorganic materials 0.000 description 1
- CHQMHPLRPQMAMX-UHFFFAOYSA-L sodium persulfate Substances [Na+].[Na+].[O-]S(=O)(=O)OOS([O-])(=O)=O CHQMHPLRPQMAMX-UHFFFAOYSA-L 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
- 239000011882 ultra-fine particle Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5277981A JPS57166559A (en) | 1981-04-07 | 1981-04-07 | Latex reagent and measuring method for latex coagulation reaction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5277981A JPS57166559A (en) | 1981-04-07 | 1981-04-07 | Latex reagent and measuring method for latex coagulation reaction |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS57166559A JPS57166559A (en) | 1982-10-14 |
| JPS6352708B2 true JPS6352708B2 (en, 2012) | 1988-10-19 |
Family
ID=12924333
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5277981A Granted JPS57166559A (en) | 1981-04-07 | 1981-04-07 | Latex reagent and measuring method for latex coagulation reaction |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS57166559A (en, 2012) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5200315A (en) * | 1990-07-25 | 1993-04-06 | Eastman Kodak Company | Particulate biologically active reagent containing polyoxyalkylene side chains, analytical element and methods for use of the reagent |
-
1981
- 1981-04-07 JP JP5277981A patent/JPS57166559A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS57166559A (en) | 1982-10-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4305925A (en) | Latex reagent | |
| JPS6243138B2 (en, 2012) | ||
| JP3623657B2 (ja) | 非特異反応抑制剤、免疫測定試薬及び免疫測定方法 | |
| US4552633A (en) | Fine particulate for use in clinical testing and a process for producing thereof | |
| JP4086266B2 (ja) | 免疫測定方法 | |
| JPH0810224B2 (ja) | 生理活性物質固定化用ラテツクス及びこのラテツクスを用いるラテツクス試薬 | |
| JPH01118770A (ja) | タンパクの測定方法 | |
| EP0064275B1 (en) | Immunochemical reagent | |
| JP3471198B2 (ja) | 免疫学的ラテックス比濁定量用試薬 | |
| JP2682697B2 (ja) | 免疫測定試薬および免疫測定法 | |
| CN101354400B (zh) | 用于胶乳聚集反应的试剂及检测目标物质的方法 | |
| JP2545707B2 (ja) | 免疫学的診断試薬 | |
| US4792527A (en) | Method of assaying biologically active substances and labelling agents therefor | |
| JPS6116942B2 (en, 2012) | ||
| JPS6352708B2 (en, 2012) | ||
| JPS6116944B2 (en, 2012) | ||
| JPS5850646B2 (ja) | 血清学的診断試薬用ラテツクスの製造方法 | |
| JPS6116941B2 (en, 2012) | ||
| JPS6352707B2 (en, 2012) | ||
| JPS6116943B2 (en, 2012) | ||
| US5989922A (en) | Method for determining rheumatoid factors and agents for carrying out the method | |
| JPS6352706B2 (en, 2012) | ||
| JP3328058B2 (ja) | 免疫診断薬の製造方法 | |
| JPS63228069A (ja) | 診断薬用ラテツクス、その製法および該ラテツクスを用いてなる診断薬 | |
| JPS6343412B2 (en, 2012) |