JPS629862B2 - - Google Patents
Info
- Publication number
- JPS629862B2 JPS629862B2 JP2620781A JP2620781A JPS629862B2 JP S629862 B2 JPS629862 B2 JP S629862B2 JP 2620781 A JP2620781 A JP 2620781A JP 2620781 A JP2620781 A JP 2620781A JP S629862 B2 JPS629862 B2 JP S629862B2
- Authority
- JP
- Japan
- Prior art keywords
- reaction
- peroxidase
- enzyme
- reaction mixture
- horseradish peroxidase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000004190 Enzymes Human genes 0.000 claims description 17
- 108090000790 Enzymes Proteins 0.000 claims description 17
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims description 17
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical group NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 claims description 13
- 239000007795 chemical reaction product Substances 0.000 claims description 13
- 239000011541 reaction mixture Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 12
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 11
- 239000000758 substrate Substances 0.000 claims description 10
- 239000003638 chemical reducing agent Substances 0.000 claims description 8
- 230000000087 stabilizing effect Effects 0.000 claims description 7
- 150000003839 salts Chemical class 0.000 claims description 6
- 230000006641 stabilisation Effects 0.000 claims description 4
- 238000011105 stabilization Methods 0.000 claims description 4
- 102000003992 Peroxidases Human genes 0.000 claims description 3
- 229940088598 enzyme Drugs 0.000 description 16
- 238000006243 chemical reaction Methods 0.000 description 14
- 239000011734 sodium Substances 0.000 description 13
- 102000013415 peroxidase activity proteins Human genes 0.000 description 9
- 239000000243 solution Substances 0.000 description 7
- HRZFUMHJMZEROT-UHFFFAOYSA-L sodium disulfite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])(=O)=O HRZFUMHJMZEROT-UHFFFAOYSA-L 0.000 description 6
- 235000010262 sodium metabisulphite Nutrition 0.000 description 6
- 239000011324 bead Substances 0.000 description 5
- 239000000872 buffer Substances 0.000 description 5
- 239000008363 phosphate buffer Substances 0.000 description 5
- 229940001584 sodium metabisulfite Drugs 0.000 description 5
- 239000003381 stabilizer Substances 0.000 description 5
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Substances [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 4
- 238000003018 immunoassay Methods 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 3
- 235000019345 sodium thiosulphate Nutrition 0.000 description 3
- GHCZTIFQWKKGSB-UHFFFAOYSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;phosphoric acid Chemical compound OP(O)(O)=O.OC(=O)CC(O)(C(O)=O)CC(O)=O GHCZTIFQWKKGSB-UHFFFAOYSA-N 0.000 description 2
- 101710132601 Capsid protein Proteins 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 238000000862 absorption spectrum Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000003398 denaturant Substances 0.000 description 2
- WBZKQQHYRPRKNJ-UHFFFAOYSA-L disulfite Chemical compound [O-]S(=O)S([O-])(=O)=O WBZKQQHYRPRKNJ-UHFFFAOYSA-L 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- RZXMPPFPUUCRFN-UHFFFAOYSA-N p-toluidine Chemical compound CC1=CC=C(N)C=C1 RZXMPPFPUUCRFN-UHFFFAOYSA-N 0.000 description 2
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 2
- 239000012429 reaction media Substances 0.000 description 2
- PTLRDCMBXHILCL-UHFFFAOYSA-M sodium arsenite Chemical compound [Na+].[O-][As]=O PTLRDCMBXHILCL-UHFFFAOYSA-M 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- CBCKQZAAMUWICA-UHFFFAOYSA-N 1,4-phenylenediamine Chemical compound NC1=CC=C(N)C=C1 CBCKQZAAMUWICA-UHFFFAOYSA-N 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 229940126062 Compound A Drugs 0.000 description 1
- 108060006006 Cytochrome-c peroxidase Proteins 0.000 description 1
- NLDMNSXOCDLTTB-UHFFFAOYSA-N Heterophylliin A Natural products O1C2COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC2C(OC(=O)C=2C=C(O)C(O)=C(O)C=2)C(O)C1OC(=O)C1=CC(O)=C(O)C(O)=C1 NLDMNSXOCDLTTB-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 108010023244 Lactoperoxidase Proteins 0.000 description 1
- 102000045576 Lactoperoxidases Human genes 0.000 description 1
- QCYOIFVBYZNUNW-BYPYZUCNSA-N N,N-dimethyl-L-alanine Chemical compound CN(C)[C@@H](C)C(O)=O QCYOIFVBYZNUNW-BYPYZUCNSA-N 0.000 description 1
- AFBPFSWMIHJQDM-UHFFFAOYSA-N N-methyl-N-phenylamine Natural products CNC1=CC=CC=C1 AFBPFSWMIHJQDM-UHFFFAOYSA-N 0.000 description 1
- 229910004879 Na2S2O5 Inorganic materials 0.000 description 1
- 108700020962 Peroxidase Proteins 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- -1 antibodies Proteins 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- AQLMHYSWFMLWBS-UHFFFAOYSA-N arsenite(1-) Chemical compound O[As](O)[O-] AQLMHYSWFMLWBS-UHFFFAOYSA-N 0.000 description 1
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- LBJNMUFDOHXDFG-UHFFFAOYSA-N copper;hydrate Chemical compound O.[Cu].[Cu] LBJNMUFDOHXDFG-UHFFFAOYSA-N 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 229940057428 lactoperoxidase Drugs 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229940079877 pyrogallol Drugs 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- DHCDFWKWKRSZHF-UHFFFAOYSA-N sulfurothioic S-acid Chemical compound OS(O)(=O)=S DHCDFWKWKRSZHF-UHFFFAOYSA-N 0.000 description 1
- 238000000954 titration curve Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12681780A | 1980-03-03 | 1980-03-03 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS56137155A JPS56137155A (en) | 1981-10-26 |
| JPS629862B2 true JPS629862B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1987-03-03 |
Family
ID=22426850
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2620781A Granted JPS56137155A (en) | 1980-03-03 | 1981-02-26 | Stabilization of resultant detectable generated during immunity test of enzime |
Country Status (5)
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63258329A (ja) * | 1987-04-13 | 1988-10-25 | Taitoo:Kk | カ−ド発行装置 |
| JPH01135573U (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1988-03-10 | 1989-09-18 | ||
| JPH0358761U (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1989-10-16 | 1991-06-07 |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3360857D1 (en) * | 1982-03-03 | 1985-10-31 | Nat Res Dev | Enhanced luminescent and luminometric assay |
| US5225325A (en) * | 1990-03-02 | 1993-07-06 | Ventana Medical Systems, Inc. | Immunohistochemical staining method and reagents therefor |
| GB9723773D0 (en) * | 1997-11-12 | 1998-01-07 | Univ Glasgow | Haptoglobin assay |
| GB9924180D0 (en) * | 1999-10-12 | 1999-12-15 | Univ Glasgow | Assays for mastitis |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL6608106A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1966-06-10 | 1967-12-11 | ||
| NL7610608A (nl) * | 1976-09-24 | 1978-03-29 | Akzo Nv | Werkwijze voor het stabiliseren van peroxidase- -bevattende composities. |
| US4234680A (en) * | 1979-08-03 | 1980-11-18 | Calbiochem-Behring Corp. | Method for terminating a peroxidase catalyzed reaction |
| US4252896A (en) * | 1980-01-07 | 1981-02-24 | Abbott Laboratories | Method of stabilizing peroxidase in a serum protein based medium |
-
1981
- 1981-02-02 GB GB8103120A patent/GB2070767B/en not_active Expired
- 1981-02-26 JP JP2620781A patent/JPS56137155A/ja active Granted
- 1981-03-02 DE DE19813107904 patent/DE3107904A1/de not_active Ceased
- 1981-03-03 BE BE0/203993A patent/BE887771A/fr not_active IP Right Cessation
- 1981-03-03 FR FR8104239A patent/FR2477176A1/fr active Granted
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63258329A (ja) * | 1987-04-13 | 1988-10-25 | Taitoo:Kk | カ−ド発行装置 |
| JPH01135573U (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1988-03-10 | 1989-09-18 | ||
| JPH0358761U (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1989-10-16 | 1991-06-07 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS56137155A (en) | 1981-10-26 |
| FR2477176A1 (fr) | 1981-09-04 |
| FR2477176B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1984-09-14 |
| BE887771A (fr) | 1981-09-03 |
| GB2070767B (en) | 1983-07-27 |
| DE3107904A1 (de) | 1982-01-21 |
| GB2070767A (en) | 1981-09-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA1155737A (en) | Process and diagnostic agents for the detection of redox reactions | |
| CA1252713A (en) | Instrumentless quantitative analysis system | |
| Arnold et al. | An automated continuous assay of membrane-bound and soluble ATPases and related enzymes | |
| EP1038939B1 (en) | Chemiluminescent reagents and chemiluminescence analysis methods with the use of the same | |
| JPS612078A (ja) | 凝集免疫検定における非特異的干渉を減少する方法 | |
| JPS629862B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | ||
| EP0198286A2 (en) | Analytical compositions, elements and methods utilizing reduction of ferric ion chelates to form detectable dyes | |
| JPS584918B2 (ja) | グルタメ−ト−オキザロアセテ−ト−トランスアミナ−ゼ及びグルタメ−ト−ピルベ−ト−トランスアミナ−ゼの測定法 | |
| JPH063447B2 (ja) | 化学的触媒の調節剤によるイムノアッセイの信号強化法 | |
| US4302537A (en) | Reagent and method for the determination of peroxidase | |
| JPH0421819B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | ||
| JP4319545B2 (ja) | 総システインアッセイ | |
| JP2000316600A (ja) | 遊離脂肪酸の定量方法及び遊離脂肪酸定量用試薬 | |
| Chen et al. | Ambivalent active-site-directed inactivators of liver alcohol dehydrogenase | |
| Hatase et al. | Specific inhibition of phosphate transport in mitochondria by N-ethylmaleimide | |
| JPS6342470A (ja) | ペルオキシダ−ゼまたはｈ↓２ｏ↓２の測定方法 | |
| JPS6252466A (ja) | 不均一イムノアッセイを行う方法 | |
| JP4120024B2 (ja) | 改良された酵素基質 | |
| JPH012598A (ja) | カルシウムの測定方法 | |
| JPH01257265A (ja) | 前立腺酸性ホスフアターゼの測定方法 | |
| CN103558213B (zh) | 一种蛋白质烷基化修饰剂peg修饰能力的检测方法及其应用 | |
| JPS62180269A (ja) | 遊離型サイロキシンの酵素免疫測定法 | |
| JPS62218865A (ja) | リウマチ因子の定量法 | |
| JPS6058095A (ja) | 血清遊離脂肪酸の定量法 | |
| JP3804102B2 (ja) | 改良された酵素基質の製造法 |