JPS6254473B2 - - Google Patents
Info
- Publication number
- JPS6254473B2 JPS6254473B2 JP58202929A JP20292983A JPS6254473B2 JP S6254473 B2 JPS6254473 B2 JP S6254473B2 JP 58202929 A JP58202929 A JP 58202929A JP 20292983 A JP20292983 A JP 20292983A JP S6254473 B2 JPS6254473 B2 JP S6254473B2
- Authority
- JP
- Japan
- Prior art keywords
- plasmid
- bacillus
- dna
- genetic marker
- microorganism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
- C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US43854482A | 1982-11-01 | 1982-11-01 | |
US438544 | 1982-11-01 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS59130187A JPS59130187A (ja) | 1984-07-26 |
JPS6254473B2 true JPS6254473B2 (en, 2012) | 1987-11-16 |
Family
ID=23741032
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP58202929A Granted JPS59130187A (ja) | 1982-11-01 | 1983-10-31 | バチルス微生物における遺伝子の異型クロ−ニング方法 |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0108301B2 (en, 2012) |
JP (1) | JPS59130187A (en, 2012) |
AT (1) | ATE48156T1 (en, 2012) |
DE (1) | DE3380878D1 (en, 2012) |
DK (1) | DK498883A (en, 2012) |
NO (1) | NO170594C (en, 2012) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5624829A (en) * | 1984-07-03 | 1997-04-29 | Gist-Brocades, B.V. | Transformed industrial bacillus strains and methods for making and using them |
CA1277931C (en) * | 1984-06-05 | 1990-12-18 | Shimon Ulitzur | Detection and/or identification of microorganisms in a test sample usingbioluminescence or other exogenous genetically-introduced marker |
DE3527913A1 (de) * | 1985-08-03 | 1987-02-12 | Henkel Kgaa | Alkalische protease, verfahren zur herstellung von hybridvektoren und genetisch transformierte mikroorganismen |
US5017477A (en) * | 1985-10-25 | 1991-05-21 | Biotechnica International, Inc. | Enhancing DNA sequencies derived from the sacQ gene |
ES2134186T3 (es) * | 1987-02-27 | 1999-10-01 | Genencor Int | Clonaje molecular y expresion de genes que codifican enzimas proteoliticas. |
JP3026567B2 (ja) * | 1987-02-27 | 2000-03-27 | ジェネンコー インターナショナル インコーポレイテッド | 分子のクローニング及びタンパク分解酵素をコードする遺伝子の発現 |
WO1997048822A1 (en) * | 1996-06-17 | 1997-12-24 | Microcide Pharmaceuticals, Inc. | Screening methods using microbial strain pools |
WO2016069432A1 (en) * | 2014-10-30 | 2016-05-06 | Merck Sharp & Dohme Corp. | Bacillus megaterium recombinant protein expression system |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2048894A (en) * | 1979-05-11 | 1980-12-17 | Gist Brocades Nv | Plasmid |
NO159863C (no) * | 1980-01-07 | 1989-02-15 | Univ Rochester | Fremgangsm te for fremstilling og seleksjon av en rant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme. |
IL61982A (en) * | 1980-02-15 | 1984-01-31 | Cpc International Inc | Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes |
ZA81424B (en) * | 1980-02-15 | 1982-02-24 | Cpc International Inc | Genetically engineered microorganisms for massive production of amylolytic enzymes and process for preparing same |
FI64813C (fi) * | 1980-12-31 | 1984-01-10 | Ilkka Antero Palva | Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer |
IE52434B1 (en) * | 1981-01-15 | 1987-10-28 | Cpc International Inc | A process for cloning the gene coding for a thermostable alpha-amylase into escherichia coli and bacillus subtilis |
JPS57139092A (en) * | 1981-02-23 | 1982-08-27 | Microbial Chem Res Found | New antibiotic istamycin a1, b1, c1 or a2 |
-
1983
- 1983-10-19 NO NO833808A patent/NO170594C/no unknown
- 1983-10-19 EP EP83110394A patent/EP0108301B2/en not_active Expired - Lifetime
- 1983-10-19 AT AT83110394T patent/ATE48156T1/de not_active IP Right Cessation
- 1983-10-19 DE DE8383110394T patent/DE3380878D1/de not_active Expired
- 1983-10-31 DK DK498883A patent/DK498883A/da not_active Application Discontinuation
- 1983-10-31 JP JP58202929A patent/JPS59130187A/ja active Granted
Also Published As
Publication number | Publication date |
---|---|
EP0108301A3 (en) | 1986-02-05 |
EP0108301B2 (en) | 1993-09-01 |
NO170594B (no) | 1992-07-27 |
NO170594C (no) | 1992-11-04 |
EP0108301B1 (en) | 1989-11-23 |
DE3380878D1 (en) | 1989-12-28 |
NO833808L (no) | 1984-05-02 |
EP0108301A2 (en) | 1984-05-16 |
JPS59130187A (ja) | 1984-07-26 |
DK498883D0 (da) | 1983-10-31 |
ATE48156T1 (de) | 1989-12-15 |
DK498883A (da) | 1984-05-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Slepecky et al. | The genus Bacillus—nonmedical | |
Lu et al. | Interactions of the components of the general secretion pathway: role of Pseudomonas aeruginosa type IV pilin subunits in complex formation and extracellular protein secretion | |
US4493893A (en) | Process for cloning the gene coding for a thermostable alpha-amylase into Escherichia coli and Bacillus subtilis | |
Ahrenholtz et al. | A conditional suicide system in Escherichia coli based on the intracellular degradation of DNA | |
EP0057976B1 (en) | a process for cloning the gene coding for a thermostable alpha-amylase into escherichia coli and bacillus subtilis | |
HU193504B (en) | Process for preparing and applying recombinated plasmids containing basic phosphatase gens | |
EP0273040A1 (en) | BIOLOGICAL CONTAINMENT. | |
US5024943A (en) | Regulatory region cloning and analysis plasmid for bacillus | |
JPS6254473B2 (en, 2012) | ||
US4374200A (en) | Broad host range small plasmid rings as cloning vehicles | |
FI112666B (fi) | Itiöimätön Bacillus subtilis, sen valmistus ja käyttö | |
NO159863B (no) | Fremgangsmaate for fremstilling og seleksjon av en rekombinant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme. | |
Bankaitis et al. | Regulation of adenylate cyclase synthesis in Escherichia coli: studies with cya-lac operon and protein fusion strains | |
US5834233A (en) | Method of limiting the survival of genetically engineered microorganisms in their enivronment | |
JPH01108978A (ja) | 中性プロテアーゼ遺伝子の分子クローニングおよび発現 | |
Szurmant et al. | The essential YycFG two‐component system of Bacillus subtilis | |
CA2173751A1 (en) | Recombinant staphylococcal nucleases and their use in limiting the survival of genetically engineered microorganisms | |
US4376164A (en) | Process for preparing broad host range small plasmid rings as cloning vehicles | |
US4418194A (en) | DNA Fragments for forming plasmids | |
JPH01503593A (ja) | 光誘導プロモーター | |
RU2103363C1 (ru) | Рекомбинантная плазмидная днк рвтn ii, определяющая синтез кристаллического инсектицидного дельта-эндотоксина cry iiia-типа, способ ее конструирования и штамм бактерий pseudomonas putida, содержащий рекомбинантную плазмиду днк рвтn ii-продуцент кристаллического инсектицидного дельта-эндотоксина cry iiia-типа, активного против насекомых отряда coleoptera | |
Rong et al. | Mutations in the precursor region of a Bacillus subtilis sporulation sigma factor | |
JPS6091984A (ja) | 新規プラスミドベクタ− | |
Kavitha et al. | Cloning and molecular characterisation of resuscitation promoting factor-like gene from Mycobacterium avium subspecies avium | |
EP0635061A1 (en) | A method of limiting the survival of genetically engineered microorganisms in their environment |