JPS6251111B2 - - Google Patents
Info
- Publication number
- JPS6251111B2 JPS6251111B2 JP59207356A JP20735684A JPS6251111B2 JP S6251111 B2 JPS6251111 B2 JP S6251111B2 JP 59207356 A JP59207356 A JP 59207356A JP 20735684 A JP20735684 A JP 20735684A JP S6251111 B2 JPS6251111 B2 JP S6251111B2
- Authority
- JP
- Japan
- Prior art keywords
- water
- oily
- decomposition products
- lipase
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000354 decomposition reaction Methods 0.000 claims description 43
- 108090001060 Lipase Proteins 0.000 claims description 40
- 102000004882 Lipase Human genes 0.000 claims description 40
- 239000004367 Lipase Substances 0.000 claims description 40
- 235000019421 lipase Nutrition 0.000 claims description 40
- 239000000758 substrate Substances 0.000 claims description 30
- 238000000034 method Methods 0.000 claims description 23
- 239000007864 aqueous solution Substances 0.000 claims description 19
- 230000007062 hydrolysis Effects 0.000 claims description 12
- 238000006460 hydrolysis reaction Methods 0.000 claims description 12
- 150000002632 lipids Chemical class 0.000 claims description 7
- 239000000047 product Substances 0.000 description 45
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 20
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 18
- 238000006243 chemical reaction Methods 0.000 description 14
- 239000000243 solution Substances 0.000 description 12
- 235000014113 dietary fatty acids Nutrition 0.000 description 11
- 239000000839 emulsion Substances 0.000 description 11
- 229930195729 fatty acid Natural products 0.000 description 11
- 239000000194 fatty acid Substances 0.000 description 11
- 150000004665 fatty acids Chemical class 0.000 description 9
- 235000011187 glycerol Nutrition 0.000 description 9
- 239000000203 mixture Substances 0.000 description 7
- 239000003921 oil Substances 0.000 description 7
- 235000019198 oils Nutrition 0.000 description 7
- 239000004006 olive oil Substances 0.000 description 7
- 235000008390 olive oil Nutrition 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 238000011084 recovery Methods 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000000499 gel Substances 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000007795 chemical reaction product Substances 0.000 description 4
- 150000002148 esters Chemical class 0.000 description 4
- 239000003925 fat Substances 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- OGBUMNBNEWYMNJ-UHFFFAOYSA-N batilol Chemical class CCCCCCCCCCCCCCCCCCOCC(O)CO OGBUMNBNEWYMNJ-UHFFFAOYSA-N 0.000 description 3
- -1 fatty acid esters Chemical class 0.000 description 3
- 230000005484 gravity Effects 0.000 description 3
- 238000002844 melting Methods 0.000 description 3
- 230000008018 melting Effects 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
- 108010093096 Immobilized Enzymes Proteins 0.000 description 2
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 2
- 235000011613 Pinus brutia Nutrition 0.000 description 2
- 241000018646 Pinus brutia Species 0.000 description 2
- AWUCVROLDVIAJX-UHFFFAOYSA-N alpha-glycerophosphate Natural products OCC(O)COP(O)(O)=O AWUCVROLDVIAJX-UHFFFAOYSA-N 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 230000003100 immobilizing effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 150000003904 phospholipids Chemical class 0.000 description 2
- 229920001467 poly(styrenesulfonates) Polymers 0.000 description 2
- AWUCVROLDVIAJX-GSVOUGTGSA-N sn-glycerol 3-phosphate Chemical compound OC[C@@H](O)COP(O)(O)=O AWUCVROLDVIAJX-GSVOUGTGSA-N 0.000 description 2
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 2
- 238000004809 thin layer chromatography Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000589513 Burkholderia cepacia Species 0.000 description 1
- 241000589540 Pseudomonas fluorescens Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 235000019626 lipase activity Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000014593 oils and fats Nutrition 0.000 description 1
- KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000009789 rate limiting process Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000007127 saponification reaction Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 235000010265 sodium sulphite Nutrition 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000008400 supply water Substances 0.000 description 1
- 239000003760 tallow Substances 0.000 description 1
- 108010072641 thermostable lipase Proteins 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59207356A JPS6185195A (ja) | 1984-10-02 | 1984-10-02 | 脂質の連続加水分解法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59207356A JPS6185195A (ja) | 1984-10-02 | 1984-10-02 | 脂質の連続加水分解法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6185195A JPS6185195A (ja) | 1986-04-30 |
| JPS6251111B2 true JPS6251111B2 (enrdf_load_stackoverflow) | 1987-10-28 |
Family
ID=16538372
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59207356A Granted JPS6185195A (ja) | 1984-10-02 | 1984-10-02 | 脂質の連続加水分解法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6185195A (enrdf_load_stackoverflow) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61287989A (ja) * | 1985-06-14 | 1986-12-18 | 花王株式会社 | 油脂の加水分解方法 |
| JPH07106154B2 (ja) * | 1986-05-28 | 1995-11-15 | 花王株式会社 | 酵素もしくは微生物反応方法 |
| JPH0198494A (ja) * | 1987-10-09 | 1989-04-17 | Agency Of Ind Science & Technol | バイオリアクター |
| US6258575B1 (en) | 1998-11-26 | 2001-07-10 | Kao Corporation | Hydrolyzing fats and oils using an immobilized enzyme column and substrate-feeding chamber that separates phases |
| JP3764855B2 (ja) | 2001-06-22 | 2006-04-12 | 花王株式会社 | 油脂類の加水分解方法 |
| JP4849967B2 (ja) | 2005-06-21 | 2012-01-11 | 花王株式会社 | 脂肪酸類の製造方法 |
| US8377664B2 (en) | 2005-10-05 | 2013-02-19 | Kao Corporation | Method for producing a useful substance by use of an immobilized enzyme |
| JP4915732B2 (ja) | 2006-12-15 | 2012-04-11 | 花王株式会社 | 固定化酵素を用いた有用物質の製造方法 |
| EP2096175A4 (en) | 2006-12-15 | 2012-04-04 | Kao Corp | METHOD FOR PRODUCING A USEFUL SUBSTANCE WITH IMMOBILIZED ENZYME |
-
1984
- 1984-10-02 JP JP59207356A patent/JPS6185195A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6185195A (ja) | 1986-04-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |