JPS62158483A - 新規アルカリプロテア−ゼａｐｇ５０１およびその製造法 - Google Patents
新規アルカリプロテア−ゼａｐｇ５０１およびその製造法Info
- Publication number
- JPS62158483A JPS62158483A JP110786A JP110786A JPS62158483A JP S62158483 A JPS62158483 A JP S62158483A JP 110786 A JP110786 A JP 110786A JP 110786 A JP110786 A JP 110786A JP S62158483 A JPS62158483 A JP S62158483A
- Authority
- JP
- Japan
- Prior art keywords
- alkaline protease
- apg501
- protease
- bacillus
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091005658 Basic proteases Proteins 0.000 title claims abstract description 40
- 238000004519 manufacturing process Methods 0.000 title claims description 8
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 17
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 claims abstract description 3
- 238000012258 culturing Methods 0.000 claims abstract description 3
- 230000000694 effects Effects 0.000 claims description 21
- 241000894006 Bacteria Species 0.000 claims description 7
- 108010013296 Sericins Proteins 0.000 claims description 7
- 108091005804 Peptidases Proteins 0.000 claims description 5
- 239000004365 Protease Substances 0.000 claims description 5
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 5
- 230000009471 action Effects 0.000 claims description 5
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 4
- MUCZHBLJLSDCSD-UHFFFAOYSA-N diisopropyl fluorophosphate Chemical compound CC(C)OP(F)(=O)OC(C)C MUCZHBLJLSDCSD-UHFFFAOYSA-N 0.000 claims description 4
- 229960005051 fluostigmine Drugs 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
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- 238000001962 electrophoresis Methods 0.000 claims description 3
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- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 2
- 239000004471 Glycine Substances 0.000 claims description 2
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 2
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims description 2
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims description 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims description 2
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- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 claims 1
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- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 8
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- 241000193747 Bacillus firmus Species 0.000 description 5
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- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 3
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
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- 235000019764 Soybean Meal Nutrition 0.000 description 2
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 2
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- 239000004455 soybean meal Substances 0.000 description 2
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- 239000012138 yeast extract Substances 0.000 description 2
- HTCSFFGLRQDZDE-UHFFFAOYSA-N 2-azaniumyl-2-phenylpropanoate Chemical compound OC(=O)C(N)(C)C1=CC=CC=C1 HTCSFFGLRQDZDE-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
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- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 239000005909 Kieselgur Substances 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
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- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
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Landscapes
- Enzymes And Modification Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP110786A JPS62158483A (ja) | 1986-01-06 | 1986-01-06 | 新規アルカリプロテア−ゼａｐｇ５０１およびその製造法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP110786A JPS62158483A (ja) | 1986-01-06 | 1986-01-06 | 新規アルカリプロテア−ゼａｐｇ５０１およびその製造法 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14492490A Division JPH0315385A (ja) | 1990-06-01 | 1990-06-01 | 新規アルカリプロテアーゼａｐｇ５０１生産菌株 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62158483A true JPS62158483A (ja) | 1987-07-14 |
| JPH0324199B2 JPH0324199B2 (enrdf_load_html_response) | 1991-04-02 |
Family
ID=11492250
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP110786A Granted JPS62158483A (ja) | 1986-01-06 | 1986-01-06 | 新規アルカリプロテア−ゼａｐｇ５０１およびその製造法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62158483A (enrdf_load_html_response) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109061123A (zh) * | 2018-06-05 | 2018-12-21 | 苏州大学 | 一种桑蚕丝含胶率定量检测方法 |
-
1986
- 1986-01-06 JP JP110786A patent/JPS62158483A/ja active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109061123A (zh) * | 2018-06-05 | 2018-12-21 | 苏州大学 | 一种桑蚕丝含胶率定量检测方法 |
| CN109061123B (zh) * | 2018-06-05 | 2020-01-10 | 苏州大学 | 一种桑蚕丝含胶率定量检测方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0324199B2 (enrdf_load_html_response) | 1991-04-02 |
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