JPS6180053A - タンパク及び核酸の銀染色法 - Google Patents

タンパク及び核酸の銀染色法

Info

Publication number
JPS6180053A
JPS6180053A JP60206713A JP20671385A JPS6180053A JP S6180053 A JPS6180053 A JP S6180053A JP 60206713 A JP60206713 A JP 60206713A JP 20671385 A JP20671385 A JP 20671385A JP S6180053 A JPS6180053 A JP S6180053A
Authority
JP
Japan
Prior art keywords
matrix
silver
formula
contacting
tables
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP60206713A
Other languages
English (en)
Japanese (ja)
Inventor
デユーク・ハイ・リー
トマス・ジヨセフ・オコンネル・サード
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of JPS6180053A publication Critical patent/JPS6180053A/ja
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827—Total protein determination, e.g. albumin in urine
    • G01N33/683—Total protein determination, e.g. albumin in urine involving metal ions
    • G01N33/6836—Silver staining
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/305—Fixative compositions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/905—Photochemical activation of reactions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Peptides Or Proteins (AREA)
JP60206713A 1984-09-21 1985-09-20 タンパク及び核酸の銀染色法 Pending JPS6180053A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/652,890 US4575452A (en) 1984-09-21 1984-09-21 Kit for silver staining proteins and nucleic acids
US652890 2007-01-12

Publications (1)

Publication Number Publication Date
JPS6180053A true JPS6180053A (ja) 1986-04-23

Family

ID=24618627

Family Applications (1)

Application Number Title Priority Date Filing Date
JP60206713A Pending JPS6180053A (ja) 1984-09-21 1985-09-20 タンパク及び核酸の銀染色法

Country Status (11)

Country Link
US (1) US4575452A (enExample)
EP (1) EP0175371B1 (enExample)
JP (1) JPS6180053A (enExample)
AT (1) ATE51078T1 (enExample)
AU (1) AU4764385A (enExample)
CA (1) CA1242626A (enExample)
DE (1) DE3576563D1 (enExample)
DK (1) DK427985A (enExample)
GR (1) GR852288B (enExample)
IL (1) IL76438A0 (enExample)
NZ (1) NZ213559A (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0280958A (ja) * 1988-09-16 1990-03-22 Dai Ichi Pure Chem Co Ltd 銀染色法及び銀染色剤キツト

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4703016A (en) * 1986-05-05 1987-10-27 The United States Of America As Represented By The Department Of Health And Human Services Silver stain for rapid, quantitative detection of polypeptides and nucleic acids
US4946794A (en) * 1986-12-18 1990-08-07 Protein Databases, Inc. Visualization of proteins on electrophoresis gels using planar dyes
US4812412A (en) * 1987-02-26 1989-03-14 Health Research Inc. Standard specimen and method of making and using same
US5064768A (en) * 1988-09-16 1991-11-12 Daiichi Pure Chemicals Co., Ltd. Silver staining technique and kit
US6074818A (en) * 1990-08-24 2000-06-13 The University Of Tennessee Research Corporation Fingerprinting of nucleic acids, products and methods
AU1893192A (en) * 1991-03-28 1992-11-02 University Of Tennessee Research Corporation, The Dna silver staining
US5422277A (en) * 1992-03-27 1995-06-06 Ortho Diagnostic Systems Inc. Cell fixative composition and method of staining cells without destroying the cell surface
US5595878A (en) * 1995-06-02 1997-01-21 Boron Biologicals, Inc. Detection of biopolymers and biooligomers with boron hydride labels
ES2308800T3 (es) * 1997-01-08 2008-12-01 Invitrogen Corporation Metodos para la produccion de proteinas.
JP2004528528A (ja) * 2000-11-20 2004-09-16 インヴィトロジェン コーポレーション マトリクス中の生体高分子の染色増強用物質
WO2007002676A2 (en) * 2005-06-27 2007-01-04 Invitrogen Corporation Liquid protein markers for native gel electrophoresis
EP2069386A4 (en) 2006-07-21 2009-10-28 Life Technologies Corp PROTEIN MOLECULAR WEIGHT STANDARDS WITH HIGH DISINFECTION
US12566174B2 (en) 2017-05-11 2026-03-03 The Research Foundation For The State University Of New York Method and kit for determining the presence of monosodium urate crystals in joint synovial fluid
CN112920174B (zh) * 2021-02-02 2022-11-04 上海交通大学 一种光敏感型化合物及其制备方法和应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB631184A (en) * 1946-10-01 1949-10-28 Eastman Kodak Co Improvements in the processing of photographic materials
US2548552A (en) * 1947-10-04 1951-04-10 Eastman Kodak Co Acidifying compound for photographic baths
CH304715A (de) * 1951-03-17 1955-01-31 Cassella Farbwerke Mainkur Ag Verfahren zur Herstellung einer fluoreszierenden heterocyclischen Verbindung.
US2696439A (en) * 1952-01-04 1954-12-07 Levinos Steven Photographic stabilization process
US3148194A (en) * 1961-07-03 1964-09-08 Arapahoe Chemicals Inc Certain 1, 4-bis[2(4-substituted, 5-phenyloxazolyl)] benzenes
US3326685A (en) * 1964-04-27 1967-06-20 Eastman Kodak Co Lithographic process using a stop bath
US3622332A (en) * 1968-04-19 1971-11-23 Itek Corp Photographic process with improved activator composition
US4405720A (en) * 1981-03-04 1983-09-20 The United States Of America As Represented By The Department Of Health And Human Services Silver stains for protein in gels
US4434234A (en) * 1981-04-02 1984-02-28 The Upjohn Company Method and kit for silver staining substances supported in matrix
US4416998A (en) * 1981-04-02 1983-11-22 The Upjohn Company Silver stain procedure and kit for same
US4459356A (en) * 1982-02-11 1984-07-10 Georgetown University Radioactive staining of gels to identify proteins
US4468466A (en) * 1982-02-16 1984-08-28 The United States Of America As Represented By The Department Of Health And Human Services Silver stains for protein in gels--a modified procedure

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0280958A (ja) * 1988-09-16 1990-03-22 Dai Ichi Pure Chem Co Ltd 銀染色法及び銀染色剤キツト

Also Published As

Publication number Publication date
EP0175371A2 (en) 1986-03-26
EP0175371A3 (en) 1986-10-01
GR852288B (enExample) 1986-01-21
DE3576563D1 (de) 1990-04-19
AU4764385A (en) 1986-03-27
DK427985D0 (da) 1985-09-20
NZ213559A (en) 1988-02-12
DK427985A (da) 1986-03-22
ATE51078T1 (de) 1990-03-15
IL76438A0 (en) 1986-01-31
CA1242626A (en) 1988-10-04
EP0175371B1 (en) 1990-03-14
US4575452A (en) 1986-03-11

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