JPS6134465A - ヒトα↓２−プラスミンインヒビタ−に対するモノクロ−ナル抗体を用いた免疫学的測定試薬及びキツト - Google Patents
ヒトα↓２−プラスミンインヒビタ−に対するモノクロ−ナル抗体を用いた免疫学的測定試薬及びキツトInfo
- Publication number
- JPS6134465A JPS6134465A JP15399384A JP15399384A JPS6134465A JP S6134465 A JPS6134465 A JP S6134465A JP 15399384 A JP15399384 A JP 15399384A JP 15399384 A JP15399384 A JP 15399384A JP S6134465 A JPS6134465 A JP S6134465A
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- plasmin
- plasmin inhibitor
- human
- monoclonal antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 101000783712 Homo sapiens Alpha-2-antiplasmin Proteins 0.000 title claims abstract description 17
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 18
- 238000003018 immunoassay Methods 0.000 title claims description 6
- 239000000126 substance Substances 0.000 claims abstract description 13
- 101000605403 Homo sapiens Plasminogen Proteins 0.000 claims abstract description 8
- 238000005259 measurement Methods 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims description 22
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 229940088598 enzyme Drugs 0.000 claims description 8
- 239000000427 antigen Substances 0.000 claims description 6
- 102000036639 antigens Human genes 0.000 claims description 6
- 108091007433 antigens Proteins 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 239000003599 detergent Substances 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 238000010324 immunological assay Methods 0.000 claims description 2
- 108010036385 plasmin-plasmin inhibitor complex Proteins 0.000 claims description 2
- 239000000758 substrate Substances 0.000 claims description 2
- 239000011324 bead Substances 0.000 claims 3
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 108090000183 alpha-2-Antiplasmin Proteins 0.000 claims 1
- 102000003801 alpha-2-Antiplasmin Human genes 0.000 claims 1
- 239000004816 latex Substances 0.000 claims 1
- 229920000126 latex Polymers 0.000 claims 1
- 230000002934 lysing effect Effects 0.000 claims 1
- 239000002923 metal particle Substances 0.000 claims 1
- 229940122791 Plasmin inhibitor Drugs 0.000 abstract description 25
- 239000002806 plasmin inhibitor Substances 0.000 abstract description 25
- 238000005406 washing Methods 0.000 abstract description 10
- 239000000523 sample Substances 0.000 abstract description 5
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 26
- 229940012957 plasmin Drugs 0.000 description 15
- 108010088842 Fibrinolysin Proteins 0.000 description 14
- 239000000243 solution Substances 0.000 description 10
- 210000004408 hybridoma Anatomy 0.000 description 7
- 206010035226 Plasma cell myeloma Diseases 0.000 description 6
- 201000000050 myeloid neoplasm Diseases 0.000 description 6
- 230000004927 fusion Effects 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 230000003480 fibrinolytic effect Effects 0.000 description 4
- 230000003053 immunization Effects 0.000 description 4
- 238000002372 labelling Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 3
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 230000027455 binding Effects 0.000 description 3
- 238000002649 immunization Methods 0.000 description 3
- 239000003112 inhibitor Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- -1 polyethylene Polymers 0.000 description 3
- 239000006152 selective media Substances 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000003146 anticoagulant agent Substances 0.000 description 2
- 239000012752 auxiliary agent Substances 0.000 description 2
- 210000001185 bone marrow Anatomy 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 239000012228 culture supernatant Substances 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229920000573 polyethylene Polymers 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 230000002537 thrombolytic effect Effects 0.000 description 2
- TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000282994 Cervidae Species 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 108090000371 Esterases Proteins 0.000 description 1
- 102000009123 Fibrin Human genes 0.000 description 1
- 108010073385 Fibrin Proteins 0.000 description 1
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000018251 Hypoxanthine Phosphoribosyltransferase Human genes 0.000 description 1
- 108010091358 Hypoxanthine Phosphoribosyltransferase Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 235000003823 Petasites japonicus Nutrition 0.000 description 1
- 240000003296 Petasites japonicus Species 0.000 description 1
- 102000013566 Plasminogen Human genes 0.000 description 1
- 108010051456 Plasminogen Proteins 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 108091006629 SLC13A2 Proteins 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 229960003896 aminopterin Drugs 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 102000005936 beta-Galactosidase Human genes 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000007910 cell fusion Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000001461 cytolytic effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- KAKKHKRHCKCAGH-UHFFFAOYSA-L disodium;(4-nitrophenyl) phosphate;hexahydrate Chemical compound O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)C1=CC=C(OP([O-])([O-])=O)C=C1 KAKKHKRHCKCAGH-UHFFFAOYSA-L 0.000 description 1
- 208000009190 disseminated intravascular coagulation Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229950003499 fibrin Drugs 0.000 description 1
- 230000020764 fibrinolysis Effects 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000000760 immunoelectrophoresis Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000007912 intraperitoneal administration Methods 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 239000000123 paper Substances 0.000 description 1
- 210000003200 peritoneal cavity Anatomy 0.000 description 1
- 102000013415 peroxidase activity proteins Human genes 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229920002239 polyacrylonitrile Polymers 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000000941 radioactive substance Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/81—Protease inhibitors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/968—Plasmin, i.e. fibrinolysin
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Neurosurgery (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15399384A JPS6134465A (ja) | 1984-07-26 | 1984-07-26 | ヒトα↓２−プラスミンインヒビタ−に対するモノクロ−ナル抗体を用いた免疫学的測定試薬及びキツト |
| DE19853586577 DE3586577T2 (de) | 1984-07-26 | 1985-07-23 | Immunologische bestimmung von menschlichem plasmin/alpha-2-plasmin-hemmstoff. |
| EP19850109227 EP0169549B1 (en) | 1984-07-26 | 1985-07-23 | Immunological determination of human plasmin/alpha2-plasmin inhibitor |
| DK339985A DK339985A (da) | 1984-07-26 | 1985-07-25 | Immunologisk bestemmelse af humanplasmin/alfa2-plasmininhibitor samt reagenssystem til brug herved |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15399384A JPS6134465A (ja) | 1984-07-26 | 1984-07-26 | ヒトα↓２−プラスミンインヒビタ−に対するモノクロ−ナル抗体を用いた免疫学的測定試薬及びキツト |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6134465A true JPS6134465A (ja) | 1986-02-18 |
| JPH0441783B2 JPH0441783B2 (enrdf_load_stackoverflow) | 1992-07-09 |
Family
ID=15574568
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15399384A Granted JPS6134465A (ja) | 1984-07-26 | 1984-07-26 | ヒトα↓２−プラスミンインヒビタ−に対するモノクロ−ナル抗体を用いた免疫学的測定試薬及びキツト |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6134465A (enrdf_load_stackoverflow) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57551A (en) * | 1980-04-30 | 1982-01-05 | Merck Patent Gmbh | Immunologic measuring method and means of enzyme |
| JPS5716355A (en) * | 1980-04-25 | 1982-01-27 | Hoffmann La Roche | Immunological method |
| JPS5794660A (en) * | 1980-09-30 | 1982-06-12 | Behringwerke Ag | Measurement of inhibitor-enzyme compound |
| JPS57136165A (en) * | 1981-02-18 | 1982-08-23 | Mochida Pharmaceut Co Ltd | Immunological measuring reagent |
| JPS58148963A (ja) * | 1982-03-02 | 1983-09-05 | Maruko Seiyaku Kk | サンドウイツチ法によるヒト体液中のアルフア−−１−アンチトリプシン・トリプシン結合物測定用試薬 |
-
1984
- 1984-07-26 JP JP15399384A patent/JPS6134465A/ja active Granted
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5716355A (en) * | 1980-04-25 | 1982-01-27 | Hoffmann La Roche | Immunological method |
| JPS57551A (en) * | 1980-04-30 | 1982-01-05 | Merck Patent Gmbh | Immunologic measuring method and means of enzyme |
| JPS5794660A (en) * | 1980-09-30 | 1982-06-12 | Behringwerke Ag | Measurement of inhibitor-enzyme compound |
| JPS57136165A (en) * | 1981-02-18 | 1982-08-23 | Mochida Pharmaceut Co Ltd | Immunological measuring reagent |
| JPS58148963A (ja) * | 1982-03-02 | 1983-09-05 | Maruko Seiyaku Kk | サンドウイツチ法によるヒト体液中のアルフア−−１−アンチトリプシン・トリプシン結合物測定用試薬 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0441783B2 (enrdf_load_stackoverflow) | 1992-07-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| EXPY | Cancellation because of completion of term |