JPS6045534A - 肝実質細胞増殖因子 - Google Patents
肝実質細胞増殖因子Info
- Publication number
- JPS6045534A JPS6045534A JP58153426A JP15342683A JPS6045534A JP S6045534 A JPS6045534 A JP S6045534A JP 58153426 A JP58153426 A JP 58153426A JP 15342683 A JP15342683 A JP 15342683A JP S6045534 A JPS6045534 A JP S6045534A
- Authority
- JP
- Japan
- Prior art keywords
- serum
- protein
- hgf
- buffer solution
- activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000003745 Hepatocyte Growth Factor Human genes 0.000 title claims abstract description 7
- 108090000100 Hepatocyte Growth Factor Proteins 0.000 title claims abstract description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 35
- 230000000694 effects Effects 0.000 claims abstract description 35
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 35
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 35
- 239000011780 sodium chloride Substances 0.000 claims abstract description 18
- 238000002523 gelfiltration Methods 0.000 claims abstract description 12
- 238000000338 in vitro Methods 0.000 claims abstract description 10
- 229920002684 Sepharose Polymers 0.000 claims abstract description 7
- 230000001766 physiological effect Effects 0.000 claims abstract description 6
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims abstract description 4
- VJHCJDRQFCCTHL-UHFFFAOYSA-N acetic acid 2,3,4,5,6-pentahydroxyhexanal Chemical compound CC(O)=O.OCC(O)C(O)C(O)C(O)C=O VJHCJDRQFCCTHL-UHFFFAOYSA-N 0.000 claims abstract description 4
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 claims abstract description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims abstract 8
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims abstract 4
- 230000002440 hepatic effect Effects 0.000 claims description 21
- 210000004738 parenchymal cell Anatomy 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 8
- 239000000872 buffer Substances 0.000 claims description 5
- 239000008363 phosphate buffer Substances 0.000 claims description 5
- 241000124008 Mammalia Species 0.000 claims description 3
- KYARBIJYVGJZLB-UHFFFAOYSA-N 7-amino-4-hydroxy-2-naphthalenesulfonic acid Chemical compound OC1=CC(S(O)(=O)=O)=CC2=CC(N)=CC=C21 KYARBIJYVGJZLB-UHFFFAOYSA-N 0.000 claims 1
- 230000002062 proliferating effect Effects 0.000 claims 1
- 210000002966 serum Anatomy 0.000 abstract description 46
- 238000011282 treatment Methods 0.000 abstract description 9
- 239000007853 buffer solution Substances 0.000 abstract description 7
- 241000283690 Bos taurus Species 0.000 abstract description 3
- 241000282414 Homo sapiens Species 0.000 abstract description 3
- 238000005119 centrifugation Methods 0.000 abstract description 3
- 238000000108 ultra-filtration Methods 0.000 abstract description 3
- 241000283073 Equus caballus Species 0.000 abstract description 2
- 238000004587 chromatography analysis Methods 0.000 abstract description 2
- 239000002808 molecular sieve Substances 0.000 abstract description 2
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 36
- 235000018102 proteins Nutrition 0.000 description 32
- 102100021866 Hepatocyte growth factor Human genes 0.000 description 19
- 230000006820 DNA synthesis Effects 0.000 description 16
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 9
- 210000004185 liver Anatomy 0.000 description 9
- 229920005654 Sephadex Polymers 0.000 description 8
- 239000003102 growth factor Substances 0.000 description 8
- 210000003494 hepatocyte Anatomy 0.000 description 8
- 230000035755 proliferation Effects 0.000 description 8
- 239000012507 Sephadex™ Substances 0.000 description 7
- 238000011160 research Methods 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- 102000004877 Insulin Human genes 0.000 description 6
- 108090001061 Insulin Proteins 0.000 description 6
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 6
- 229940125396 insulin Drugs 0.000 description 6
- 241000700159 Rattus Species 0.000 description 5
- 229940088597 hormone Drugs 0.000 description 5
- 239000005556 hormone Substances 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 5
- 102000009024 Epidermal Growth Factor Human genes 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 238000010828 elution Methods 0.000 description 4
- 238000005194 fractionation Methods 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 108090000190 Thrombin Proteins 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 238000000862 absorption spectrum Methods 0.000 description 3
- 238000000502 dialysis Methods 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000012753 partial hepatectomy Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 229960004072 thrombin Drugs 0.000 description 3
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 101100136092 Drosophila melanogaster peng gene Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 102000048143 Insulin-Like Growth Factor II Human genes 0.000 description 2
- 108090001117 Insulin-Like Growth Factor II Proteins 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 229960002897 heparin Drugs 0.000 description 2
- 229920000669 heparin Polymers 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 230000003908 liver function Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- -1 propatool Chemical compound 0.000 description 2
- 235000004252 protein component Nutrition 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- 239000011800 void material Substances 0.000 description 2
- 101800000263 Acidic protein Proteins 0.000 description 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 229920002160 Celluloid Polymers 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 101800003838 Epidermal growth factor Proteins 0.000 description 1
- 238000012752 Hepatectomy Methods 0.000 description 1
- VSNHCAURESNICA-UHFFFAOYSA-N Hydroxyurea Chemical compound NC(=O)NO VSNHCAURESNICA-UHFFFAOYSA-N 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 102000010780 Platelet-Derived Growth Factor Human genes 0.000 description 1
- 108010038512 Platelet-Derived Growth Factor Proteins 0.000 description 1
- 241000700157 Rattus norvegicus Species 0.000 description 1
- 239000012506 Sephacryl® Substances 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 102000004338 Transferrin Human genes 0.000 description 1
- 108090000901 Transferrin Proteins 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 101710112926 Trypsin inhibitor 4 Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- MZVQCMJNVPIDEA-UHFFFAOYSA-N [CH2]CN(CC)CC Chemical group [CH2]CN(CC)CC MZVQCMJNVPIDEA-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000010306 acid treatment Methods 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 210000000709 aorta Anatomy 0.000 description 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000003145 cytotoxic factor Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 1
- 229960003957 dexamethasone Drugs 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940116977 epidermal growth factor Drugs 0.000 description 1
- NJSUFZNXBBXAAC-UHFFFAOYSA-N ethanol;toluene Chemical compound CCO.CC1=CC=CC=C1 NJSUFZNXBBXAAC-UHFFFAOYSA-N 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 229960001330 hydroxycarbamide Drugs 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- KKHLKORVTUUSBC-YFKPBYRVSA-N n-(2,4-dinitrophenyl)-l-alanine Chemical compound OC(=O)[C@H](C)NC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O KKHLKORVTUUSBC-YFKPBYRVSA-N 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 235000011008 sodium phosphates Nutrition 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000012134 supernatant fraction Substances 0.000 description 1
- 229940126585 therapeutic drug Drugs 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 239000012581 transferrin Substances 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP58153426A JPS6045534A (ja) | 1983-08-22 | 1983-08-22 | 肝実質細胞増殖因子 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP58153426A JPS6045534A (ja) | 1983-08-22 | 1983-08-22 | 肝実質細胞増殖因子 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS6045534A true JPS6045534A (ja) | 1985-03-12 |
JPH0472840B2 JPH0472840B2 (enrdf_load_stackoverflow) | 1992-11-19 |
Family
ID=15562249
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP58153426A Granted JPS6045534A (ja) | 1983-08-22 | 1983-08-22 | 肝実質細胞増殖因子 |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6045534A (enrdf_load_stackoverflow) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6322526A (ja) * | 1986-07-14 | 1988-01-30 | Shuji Hashimoto | 肝細胞増殖因子 |
JPS643199A (en) * | 1987-06-25 | 1989-01-06 | Yasushi Daikuhara | Monoclonal antibody |
WO1993008821A1 (en) * | 1991-11-07 | 1993-05-13 | Toshikazu Nakamura | Side effect inhibitor for cancer therapy |
US5547856A (en) * | 1992-05-18 | 1996-08-20 | Genentech, Inc. | Hepatocyte growth factor variants |
US5580963A (en) * | 1992-05-18 | 1996-12-03 | Genentech, Inc. | Single-chain hepatocyte growth factor variants |
US5736506A (en) * | 1991-06-03 | 1998-04-07 | Mitsubishi Kasei Corporation | Method of inhibiting the degradation of hepatocyte growth factor |
US5879910A (en) * | 1992-05-18 | 1999-03-09 | Genetech, Inc. | Hepatocyte growth factor protease domain variants |
US6033688A (en) * | 1995-09-12 | 2000-03-07 | Genentech, Inc. | Cystic fibrosis therapy |
JP2009524622A (ja) * | 2006-01-25 | 2009-07-02 | オクタファルマ アクチェン ゲゼルシャフト | 創傷治癒支援因子の精製および使用 |
-
1983
- 1983-08-22 JP JP58153426A patent/JPS6045534A/ja active Granted
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6322526A (ja) * | 1986-07-14 | 1988-01-30 | Shuji Hashimoto | 肝細胞増殖因子 |
JPS643199A (en) * | 1987-06-25 | 1989-01-06 | Yasushi Daikuhara | Monoclonal antibody |
US5736506A (en) * | 1991-06-03 | 1998-04-07 | Mitsubishi Kasei Corporation | Method of inhibiting the degradation of hepatocyte growth factor |
WO1993008821A1 (en) * | 1991-11-07 | 1993-05-13 | Toshikazu Nakamura | Side effect inhibitor for cancer therapy |
US5547856A (en) * | 1992-05-18 | 1996-08-20 | Genentech, Inc. | Hepatocyte growth factor variants |
US5580963A (en) * | 1992-05-18 | 1996-12-03 | Genentech, Inc. | Single-chain hepatocyte growth factor variants |
US5879910A (en) * | 1992-05-18 | 1999-03-09 | Genetech, Inc. | Hepatocyte growth factor protease domain variants |
US6033688A (en) * | 1995-09-12 | 2000-03-07 | Genentech, Inc. | Cystic fibrosis therapy |
JP2009524622A (ja) * | 2006-01-25 | 2009-07-02 | オクタファルマ アクチェン ゲゼルシャフト | 創傷治癒支援因子の精製および使用 |
Also Published As
Publication number | Publication date |
---|---|
JPH0472840B2 (enrdf_load_stackoverflow) | 1992-11-19 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Gohda et al. | Purification and partial characterization of hepatocyte growth factor from plasma of a patient with fulminant hepatic failure. | |
Moghaddam et al. | Expression of platelet-derived endothelial cell growth factor in Escherichia coli and confirmation of its thymidine phosphorylase activity | |
Russell et al. | Partial characterization of a hepatocyte growth factor from rat platelets | |
Nakamura et al. | Partial purification and characterization of human hepatoma-derived growth factor | |
US5004805A (en) | Hepatocyte growth factor | |
Fett et al. | Isolation and characterization of angiogenin, an angiogenic protein from human carcinoma cells | |
Matsuoka et al. | Stimulation of hepatic lipocyte collagen production by Kupffer cell‐derived transforming growth factor β: implication for a pathogenetic role in alcoholic liver fibrogenesis | |
Greenberg et al. | Transglutaminases: multifunctional cross‐linking enzymes that stabilize tissues | |
Zetter et al. | Stimulation of human vascular endothelial cell growth by a platelet‐derived growth factor and thrombin | |
JPH0449246A (ja) | 賢疾患治療剤 | |
Miyazono et al. | A platelet factor that stimulates the proliferation of vascular endothelial cells | |
JPS6045534A (ja) | 肝実質細胞増殖因子 | |
JPH0386900A (ja) | 新規なトロンビン結合性物質及びその製法 | |
JPH03195496A (ja) | 成長因子 | |
JPH0372429A (ja) | 血小板減少症の治療剤 | |
JPH02117698A (ja) | 血管内皮細胞成長因子 | |
Gohda et al. | Human hepatocyte growth factor in blood of patients with fulminant hepatic failure: basic aspects | |
JPH04352800A (ja) | 平滑筋分裂促進因子 | |
Nakamura et al. | Two types of growth inhibitor in rat platelets for primary cultured rat hepatocytes | |
JPH0635480B2 (ja) | 肝実質細胞増殖因子 | |
Chiu et al. | Placental keratinocyte growth factor: partial purification and comparison with epidermal growth factor | |
JPH06508111A (ja) | 甲状腺由来の軟骨細胞刺激因子 | |
Samarut | Isolation of an erythropoietic stimulating factor from the serum of anemic chicks | |
Imaizumi et al. | Non-heparin-binding endothelial cell growth factor from bovine omentum | |
Nagayama et al. | Mouse bone collagenase inhibitor: purification and partial characterization of the inhibitor from mouse calvaria cultures |