JPS6043381A - 固定化生物活性物質の製造法 - Google Patents
固定化生物活性物質の製造法Info
- Publication number
- JPS6043381A JPS6043381A JP15267583A JP15267583A JPS6043381A JP S6043381 A JPS6043381 A JP S6043381A JP 15267583 A JP15267583 A JP 15267583A JP 15267583 A JP15267583 A JP 15267583A JP S6043381 A JPS6043381 A JP S6043381A
- Authority
- JP
- Japan
- Prior art keywords
- biologically active
- active substance
- aqueous solution
- polymerization
- vinyl monomer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004519 manufacturing process Methods 0.000 title claims description 12
- 239000000126 substance Substances 0.000 title abstract description 13
- 230000000975 bioactive effect Effects 0.000 title abstract description 11
- 239000007864 aqueous solution Substances 0.000 claims abstract description 37
- 239000000178 monomer Substances 0.000 claims abstract description 25
- 238000006116 polymerization reaction Methods 0.000 claims abstract description 25
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 claims abstract description 25
- 229920002554 vinyl polymer Polymers 0.000 claims abstract description 25
- 102000004190 Enzymes Human genes 0.000 claims abstract description 9
- 108090000790 Enzymes Proteins 0.000 claims abstract description 9
- 229920000642 polymer Polymers 0.000 claims abstract description 9
- 230000001678 irradiating effect Effects 0.000 claims abstract description 8
- 244000005700 microbiome Species 0.000 claims abstract description 6
- 229940088623 biologically active substance Drugs 0.000 claims description 34
- 239000012528 membrane Substances 0.000 claims description 19
- 230000003100 immobilizing effect Effects 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 11
- 229940088598 enzyme Drugs 0.000 abstract description 7
- 238000006243 chemical reaction Methods 0.000 abstract description 6
- 241000589540 Pseudomonas fluorescens Species 0.000 abstract description 3
- 239000004366 Glucose oxidase Substances 0.000 abstract description 2
- 108010015776 Glucose oxidase Proteins 0.000 abstract description 2
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 abstract description 2
- 229940116332 glucose oxidase Drugs 0.000 abstract description 2
- 235000019420 glucose oxidase Nutrition 0.000 abstract description 2
- 239000000243 solution Substances 0.000 abstract description 2
- 239000013543 active substance Substances 0.000 description 9
- 238000000034 method Methods 0.000 description 8
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 4
- 229920005597 polymer membrane Polymers 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 108010093096 Immobilized Enzymes Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 2
- 239000003431 cross linking reagent Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000020169 heat generation Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 2
- -1 pericillinase Proteins 0.000 description 2
- 229920002635 polyurethane Polymers 0.000 description 2
- 239000004814 polyurethane Substances 0.000 description 2
- 238000001223 reverse osmosis Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 229920000536 2-Acrylamido-2-methylpropane sulfonic acid Polymers 0.000 description 1
- XHZPRMZZQOIPDS-UHFFFAOYSA-N 2-Methyl-2-[(1-oxo-2-propenyl)amino]-1-propanesulfonic acid Chemical compound OS(=O)(=O)CC(C)(C)NC(=O)C=C XHZPRMZZQOIPDS-UHFFFAOYSA-N 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 108091005658 Basic proteases Proteins 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 229920002284 Cellulose triacetate Polymers 0.000 description 1
- 108010089254 Cholesterol oxidase Proteins 0.000 description 1
- 108010066906 Creatininase Proteins 0.000 description 1
- 108010073324 Glutaminase Proteins 0.000 description 1
- 102000009127 Glutaminase Human genes 0.000 description 1
- WOBHKFSMXKNTIM-UHFFFAOYSA-N Hydroxyethyl methacrylate Chemical compound CC(=C)C(=O)OCCO WOBHKFSMXKNTIM-UHFFFAOYSA-N 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 239000004642 Polyimide Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 241000235527 Rhizopus Species 0.000 description 1
- 241000187392 Streptomyces griseus Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000223230 Trichosporon Species 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 108010092464 Urate Oxidase Proteins 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- 108700040099 Xylose isomerases Proteins 0.000 description 1
- NNLVGZFZQQXQNW-ADJNRHBOSA-N [(2r,3r,4s,5r,6s)-4,5-diacetyloxy-3-[(2s,3r,4s,5r,6r)-3,4,5-triacetyloxy-6-(acetyloxymethyl)oxan-2-yl]oxy-6-[(2r,3r,4s,5r,6s)-4,5,6-triacetyloxy-2-(acetyloxymethyl)oxan-3-yl]oxyoxan-2-yl]methyl acetate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](OC(C)=O)[C@H]1OC(C)=O)O[C@H]1[C@@H]([C@@H](OC(C)=O)[C@H](OC(C)=O)[C@@H](COC(C)=O)O1)OC(C)=O)COC(=O)C)[C@@H]1[C@@H](COC(C)=O)O[C@@H](OC(C)=O)[C@H](OC(C)=O)[C@H]1OC(C)=O NNLVGZFZQQXQNW-ADJNRHBOSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 102000020006 aldose 1-epimerase Human genes 0.000 description 1
- 108091022872 aldose 1-epimerase Proteins 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000001573 invertase Substances 0.000 description 1
- 235000011073 invertase Nutrition 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 229920002492 poly(sulfone) Polymers 0.000 description 1
- 229920002239 polyacrylonitrile Polymers 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920001721 polyimide Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15267583A JPS6043381A (ja) | 1983-08-22 | 1983-08-22 | 固定化生物活性物質の製造法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15267583A JPS6043381A (ja) | 1983-08-22 | 1983-08-22 | 固定化生物活性物質の製造法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6043381A true JPS6043381A (ja) | 1985-03-07 |
| JPH0320233B2 JPH0320233B2 (enrdf_load_stackoverflow) | 1991-03-18 |
Family
ID=15545646
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15267583A Granted JPS6043381A (ja) | 1983-08-22 | 1983-08-22 | 固定化生物活性物質の製造法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6043381A (enrdf_load_stackoverflow) |
-
1983
- 1983-08-22 JP JP15267583A patent/JPS6043381A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0320233B2 (enrdf_load_stackoverflow) | 1991-03-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4273873A (en) | Preparation of antithrombogenic polymeric materials | |
| US3962038A (en) | Preparation of water-insoluble enzymes | |
| EP0562371B1 (de) | Immobilisierung biochemischer Substanzen | |
| Bandhyopadhyay et al. | Solid matrix characterization of immobilized Pseudomonas putida MTCC 1194 used for phenol degradation | |
| EP0562373A2 (de) | Immobilisierung biochemischer Substanzen | |
| US4999295A (en) | Biocatalyst entrapped in a silk fibroin membrane | |
| JPS58187188A (ja) | 酵素活性物質の固定化法 | |
| JPS6043381A (ja) | 固定化生物活性物質の製造法 | |
| JPH0318877B2 (enrdf_load_stackoverflow) | ||
| CA1036965A (en) | Method of immobilizing an enzyme | |
| JPH01265886A (ja) | 繊維、フィルム状固定化酵素用担体の製造法 | |
| JP2658211B2 (ja) | 生理活性物質固定化用担体の製造法 | |
| Hsiue et al. | Preparation of glucose oxidase membrane for the application of glucose sensor by plasma activation | |
| JP2004105065A (ja) | 生物学的活性体の固定化材およびそれを用いた測定法 | |
| JPH0533720B2 (enrdf_load_stackoverflow) | ||
| GB1560691A (en) | Enzyme membrane | |
| JPS5998690A (ja) | 酵素固定化膜の架橋化方法 | |
| JPH01262794A (ja) | 繊維、フィルム状固定化酵素用担体 | |
| JPS6344885A (ja) | 酵素固定化ダイナミツクス膜 | |
| JPS6115900A (ja) | 変性セルロ−ス系多孔質膜 | |
| JPS62180787A (ja) | 酵素含有ろ過材及びその製法及びその使用方法 | |
| JP2944194B2 (ja) | 固定化酵素の製造法 | |
| JPS6157628A (ja) | 生理活性物質固定化膜の製造法 | |
| JPH0575430B2 (enrdf_load_stackoverflow) | ||
| JPH0453490A (ja) | 生理活性物質を固定化した担持物とその製造方法 |