JPS5999257A - 抗体検出用試薬の水性溶媒 - Google Patents
抗体検出用試薬の水性溶媒Info
- Publication number
- JPS5999257A JPS5999257A JP21024182A JP21024182A JPS5999257A JP S5999257 A JPS5999257 A JP S5999257A JP 21024182 A JP21024182 A JP 21024182A JP 21024182 A JP21024182 A JP 21024182A JP S5999257 A JPS5999257 A JP S5999257A
- Authority
- JP
- Japan
- Prior art keywords
- aqueous solvent
- antigen
- antibody
- reaction
- antibody detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003125 aqueous solvent Substances 0.000 title claims description 19
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 18
- 238000001514 detection method Methods 0.000 title claims description 13
- 230000001580 bacterial effect Effects 0.000 claims description 22
- 238000000034 method Methods 0.000 claims description 21
- 241000894006 Bacteria Species 0.000 claims description 19
- 238000006243 chemical reaction Methods 0.000 claims description 19
- 239000000284 extract Substances 0.000 claims description 14
- 230000004520 agglutination Effects 0.000 claims description 11
- 241000588724 Escherichia coli Species 0.000 claims description 10
- 102000004190 Enzymes Human genes 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 7
- 230000008105 immune reaction Effects 0.000 claims description 5
- 238000003127 radioimmunoassay Methods 0.000 claims description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 244000063299 Bacillus subtilis Species 0.000 claims description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 3
- 239000000427 antigen Substances 0.000 description 25
- 102000036639 antigens Human genes 0.000 description 25
- 108091007433 antigens Proteins 0.000 description 25
- 239000006286 aqueous extract Substances 0.000 description 9
- 241001494479 Pecora Species 0.000 description 8
- 210000003743 erythrocyte Anatomy 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 230000006798 recombination Effects 0.000 description 7
- 210000002966 serum Anatomy 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 239000002504 physiological saline solution Substances 0.000 description 6
- 239000002904 solvent Substances 0.000 description 6
- 210000000601 blood cell Anatomy 0.000 description 5
- 238000005215 recombination Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 4
- 210000003850 cellular structure Anatomy 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 230000035931 haemagglutination Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- QYSGYZVSCZSLHT-UHFFFAOYSA-N octafluoropropane Chemical compound FC(F)(F)C(F)(F)C(F)(F)F QYSGYZVSCZSLHT-UHFFFAOYSA-N 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000002525 ultrasonication Methods 0.000 description 2
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 206010029719 Nonspecific reaction Diseases 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000008451 emotion Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000000644 isotonic solution Substances 0.000 description 1
- 210000005228 liver tissue Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Communicable Diseases (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21024182A JPS5999257A (ja) | 1982-11-29 | 1982-11-29 | 抗体検出用試薬の水性溶媒 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21024182A JPS5999257A (ja) | 1982-11-29 | 1982-11-29 | 抗体検出用試薬の水性溶媒 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5999257A true JPS5999257A (ja) | 1984-06-07 |
| JPH0359382B2 JPH0359382B2 (enrdf_load_stackoverflow) | 1991-09-10 |
Family
ID=16586116
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP21024182A Granted JPS5999257A (ja) | 1982-11-29 | 1982-11-29 | 抗体検出用試薬の水性溶媒 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5999257A (enrdf_load_stackoverflow) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010101157A1 (ja) | 2009-03-02 | 2010-09-10 | 日本たばこ産業株式会社 | 生物学的試料中の物質を検出する方法 |
| WO2010114031A1 (ja) | 2009-03-31 | 2010-10-07 | 日本たばこ産業株式会社 | 生物学的試料中の物質を検出する方法 |
| WO2010114029A1 (ja) | 2009-03-31 | 2010-10-07 | 日本たばこ産業株式会社 | 生物学的試料中のｓｉｔｈ－１の抗体を検出する方法 |
-
1982
- 1982-11-29 JP JP21024182A patent/JPS5999257A/ja active Granted
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010101157A1 (ja) | 2009-03-02 | 2010-09-10 | 日本たばこ産業株式会社 | 生物学的試料中の物質を検出する方法 |
| WO2010114031A1 (ja) | 2009-03-31 | 2010-10-07 | 日本たばこ産業株式会社 | 生物学的試料中の物質を検出する方法 |
| WO2010114029A1 (ja) | 2009-03-31 | 2010-10-07 | 日本たばこ産業株式会社 | 生物学的試料中のｓｉｔｈ－１の抗体を検出する方法 |
| US9034590B2 (en) | 2009-03-31 | 2015-05-19 | Japan Tobacco Inc. | Method for detecting substance in biological sample |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0359382B2 (enrdf_load_stackoverflow) | 1991-09-10 |
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