JPS5992353A - 不溶性担体粒子を用いる凝集反応測定方法 - Google Patents
不溶性担体粒子を用いる凝集反応測定方法Info
- Publication number
- JPS5992353A JPS5992353A JP20216682A JP20216682A JPS5992353A JP S5992353 A JPS5992353 A JP S5992353A JP 20216682 A JP20216682 A JP 20216682A JP 20216682 A JP20216682 A JP 20216682A JP S5992353 A JPS5992353 A JP S5992353A
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- antigen
- insoluble carrier
- reaction
- serum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 23
- 239000002245 particle Substances 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims description 16
- 230000003311 flocculating effect Effects 0.000 title abstract 2
- 230000004520 agglutination Effects 0.000 claims description 12
- 238000004220 aggregation Methods 0.000 claims description 11
- 230000002776 aggregation Effects 0.000 claims description 11
- 239000011541 reaction mixture Substances 0.000 claims description 2
- 239000000427 antigen Substances 0.000 abstract description 25
- 102000036639 antigens Human genes 0.000 abstract description 25
- 108091007433 antigens Proteins 0.000 abstract description 25
- 210000002966 serum Anatomy 0.000 abstract description 18
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 abstract description 4
- 238000002835 absorbance Methods 0.000 abstract description 4
- 239000003085 diluting agent Substances 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 4
- 241001465754 Metazoa Species 0.000 abstract description 3
- 239000007853 buffer solution Substances 0.000 abstract description 3
- 238000007865 diluting Methods 0.000 abstract description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 abstract description 2
- 241000894006 Bacteria Species 0.000 abstract description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 abstract description 2
- 239000010419 fine particle Substances 0.000 abstract description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract description 2
- 229910052751 metal Inorganic materials 0.000 abstract description 2
- 239000002184 metal Substances 0.000 abstract description 2
- 150000002739 metals Chemical class 0.000 abstract description 2
- 239000011707 mineral Substances 0.000 abstract description 2
- 239000002504 physiological saline solution Substances 0.000 abstract description 2
- 239000000843 powder Substances 0.000 abstract description 2
- 241001478240 Coccus Species 0.000 abstract 1
- 241000191940 Staphylococcus Species 0.000 abstract 1
- 241000194017 Streptococcus Species 0.000 abstract 1
- 239000007795 chemical reaction product Substances 0.000 abstract 1
- 229910052809 inorganic oxide Inorganic materials 0.000 abstract 1
- 229920000620 organic polymer Polymers 0.000 abstract 1
- 239000004816 latex Substances 0.000 description 15
- 229920000126 latex Polymers 0.000 description 15
- 238000005259 measurement Methods 0.000 description 11
- 239000003153 chemical reaction reagent Substances 0.000 description 7
- 241000283973 Oryctolagus cuniculus Species 0.000 description 5
- 238000010790 dilution Methods 0.000 description 5
- 239000012895 dilution Substances 0.000 description 5
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 108060003951 Immunoglobulin Proteins 0.000 description 4
- 102000018358 immunoglobulin Human genes 0.000 description 4
- 239000008363 phosphate buffer Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000000405 serological effect Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000004471 Glycine Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 229940072221 immunoglobulins Drugs 0.000 description 2
- 238000000691 measurement method Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001235 sensitizing effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- 108010075016 Ceruloplasmin Proteins 0.000 description 1
- 102100023321 Ceruloplasmin Human genes 0.000 description 1
- 241000283977 Oryctolagus Species 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 241000295644 Staphylococcaceae Species 0.000 description 1
- 102000004338 Transferrin Human genes 0.000 description 1
- 108090000901 Transferrin Proteins 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000009918 complex formation Effects 0.000 description 1
- -1 de-IgG Proteins 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 231100000517 death Toxicity 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 238000007720 emulsion polymerization reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920002285 poly(styrene-co-acrylonitrile) Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 239000012581 transferrin Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20216682A JPS5992353A (ja) | 1982-11-19 | 1982-11-19 | 不溶性担体粒子を用いる凝集反応測定方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP20216682A JPS5992353A (ja) | 1982-11-19 | 1982-11-19 | 不溶性担体粒子を用いる凝集反応測定方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5992353A true JPS5992353A (ja) | 1984-05-28 |
| JPH0331227B2 JPH0331227B2 (enrdf_load_stackoverflow) | 1991-05-02 |
Family
ID=16453050
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP20216682A Granted JPS5992353A (ja) | 1982-11-19 | 1982-11-19 | 不溶性担体粒子を用いる凝集反応測定方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5992353A (enrdf_load_stackoverflow) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62168051A (ja) * | 1986-01-17 | 1987-07-24 | Green Cross Corp:The | 凝集反応試験用水性溶媒 |
| JPS63115061A (ja) * | 1986-10-31 | 1988-05-19 | Sekisui Chem Co Ltd | 免疫測定法 |
| JPH06324043A (ja) * | 1993-03-23 | 1994-11-25 | Boehringer Mannheim Gmbh | 粒子状キャリヤー物質での免疫試験におけるフック作用 の低減 |
| CN103293299A (zh) * | 2012-02-24 | 2013-09-11 | 上海新波生物技术有限公司 | 拓宽双抗体夹心免疫检测浓度范围的方法及其试剂盒 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114689844A (zh) * | 2020-12-31 | 2022-07-01 | 广东菲鹏生物有限公司 | 一种胶乳免疫比浊胶乳试剂及制备方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5631648A (en) * | 1979-06-19 | 1981-03-31 | Siber George | Test in which cohesion is caused by contact with body fluid containing antigen and method of preparing reagent for said test |
| JPS579723A (en) * | 1980-06-20 | 1982-01-19 | Mitsubishi Chem Ind Ltd | Stabilizing agent for immunological reaction and measuring method of antigen-antibody reaction |
| JPS57111446A (en) * | 1980-12-29 | 1982-07-10 | Sekisui Chem Co Ltd | Determing method of latex agglutination |
-
1982
- 1982-11-19 JP JP20216682A patent/JPS5992353A/ja active Granted
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5631648A (en) * | 1979-06-19 | 1981-03-31 | Siber George | Test in which cohesion is caused by contact with body fluid containing antigen and method of preparing reagent for said test |
| JPS579723A (en) * | 1980-06-20 | 1982-01-19 | Mitsubishi Chem Ind Ltd | Stabilizing agent for immunological reaction and measuring method of antigen-antibody reaction |
| JPS57111446A (en) * | 1980-12-29 | 1982-07-10 | Sekisui Chem Co Ltd | Determing method of latex agglutination |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62168051A (ja) * | 1986-01-17 | 1987-07-24 | Green Cross Corp:The | 凝集反応試験用水性溶媒 |
| JPS63115061A (ja) * | 1986-10-31 | 1988-05-19 | Sekisui Chem Co Ltd | 免疫測定法 |
| JPH06324043A (ja) * | 1993-03-23 | 1994-11-25 | Boehringer Mannheim Gmbh | 粒子状キャリヤー物質での免疫試験におけるフック作用 の低減 |
| CN103293299A (zh) * | 2012-02-24 | 2013-09-11 | 上海新波生物技术有限公司 | 拓宽双抗体夹心免疫检测浓度范围的方法及其试剂盒 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0331227B2 (enrdf_load_stackoverflow) | 1991-05-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4080264A (en) | Immunoassay by light scattering spectroscopy | |
| EP0005978A1 (en) | Process for detecting and quantifying an antigen or antibody | |
| JPS6314783B2 (enrdf_load_stackoverflow) | ||
| JPS6362699B2 (enrdf_load_stackoverflow) | ||
| US20110081731A1 (en) | Method of Assaying Antigen and Reagent Therefor | |
| JP4033853B2 (ja) | リガンドの検出のための固相アッセイ | |
| JPS6365369A (ja) | 抗原−抗体反応の測定法 | |
| JPS5992353A (ja) | 不溶性担体粒子を用いる凝集反応測定方法 | |
| JP3513075B2 (ja) | 免疫測定法及びそのための試薬 | |
| JPH01301165A (ja) | 免疫測定法 | |
| JPS61280568A (ja) | 体液成分の測定方法 | |
| JPH0712818A (ja) | 免疫学的検出方法 | |
| JP4507439B2 (ja) | ラテックス免疫比濁測定法及びそれに用いるキット | |
| JPH0448265A (ja) | 免疫測定法 | |
| JPH01158354A (ja) | 免疫学的多成分測定用試薬と測定方法 | |
| JPH0783929A (ja) | 抗原または抗体濃度の測定方法 | |
| JPS5896251A (ja) | 抗原又は抗体の測定方法および測定用試薬 | |
| JPH0560757A (ja) | 抗原の定量法及び抗原定量用免疫試薬 | |
| JPS6262291B2 (enrdf_load_stackoverflow) | ||
| JPH03118472A (ja) | インスリン定量方法及び定量試薬 | |
| JPH01253654A (ja) | 安定なラテックス試薬 | |
| JPS6293663A (ja) | 抗原又は抗体濃度の測定方法 | |
| JPS63115061A (ja) | 免疫測定法 | |
| JPS63149564A (ja) | 抗原抗体反応の判定法 | |
| CN114324855A (zh) | 糖类抗原ca72-4的检测试剂盒 |