JPS57111446A - Determing method of latex agglutination - Google Patents

Determing method of latex agglutination

Info

Publication number
JPS57111446A
JPS57111446A JP18535980A JP18535980A JPS57111446A JP S57111446 A JPS57111446 A JP S57111446A JP 18535980 A JP18535980 A JP 18535980A JP 18535980 A JP18535980 A JP 18535980A JP S57111446 A JPS57111446 A JP S57111446A
Authority
JP
Japan
Prior art keywords
latex
antigen
antibody
reagent
styrene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP18535980A
Other languages
Japanese (ja)
Other versions
JPS6352706B2 (en
Inventor
Toichi Yamada
Michio Kuge
Satoshi Obana
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sekisui Chemical Co Ltd
Original Assignee
Sekisui Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sekisui Chemical Co Ltd filed Critical Sekisui Chemical Co Ltd
Priority to JP18535980A priority Critical patent/JPS57111446A/en
Publication of JPS57111446A publication Critical patent/JPS57111446A/en
Publication of JPS6352706B2 publication Critical patent/JPS6352706B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form

Abstract

PURPOSE:To determine optically an antigen-antibody reaction with high sensibility without accompanying nonspecific agglutination, by using a latex reagent obtained by treatment of styrene copolymer latex. CONSTITUTION:Styrene and <=10wt% of that of sodium styrene sulphonate is copolymerized in an aqueous medium at 50-100 deg.C for about 5-50hr using a persulphate (for example sodium persulphate) as an initiator in the absence of an emulsifier. The obtained latex is heated at about 50-100 deg.C for about 15-100hr on an alkaline condition of about 7.5-12.5% pH to form a single dispersion latex having 0.05-2mu average particle size. The latex is sensitized by contacting with an antibody (or antigen) at about 20-37 deg.C in an aqueous solvent, and treated with a serum contg. sensitized antibody (or antigen), that is an antiserum to obtaind a latex reagent. The antigen-antibody reaction is determined optically by using the above-mentioned reagent.
JP18535980A 1980-12-29 1980-12-29 Determing method of latex agglutination Granted JPS57111446A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP18535980A JPS57111446A (en) 1980-12-29 1980-12-29 Determing method of latex agglutination

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP18535980A JPS57111446A (en) 1980-12-29 1980-12-29 Determing method of latex agglutination

Publications (2)

Publication Number Publication Date
JPS57111446A true JPS57111446A (en) 1982-07-10
JPS6352706B2 JPS6352706B2 (en) 1988-10-19

Family

ID=16169405

Family Applications (1)

Application Number Title Priority Date Filing Date
JP18535980A Granted JPS57111446A (en) 1980-12-29 1980-12-29 Determing method of latex agglutination

Country Status (1)

Country Link
JP (1) JPS57111446A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5992353A (en) * 1982-11-19 1984-05-28 Shinotesuto Kenkyusho:Kk Measuring method of flocculating reaction using insoluble carrier particle
KR20000063268A (en) * 2000-06-13 2000-11-06 김형순 Model Colloid for Diagnostic Usage, and Method for Producing Thereof
CN104685359A (en) * 2012-07-31 2015-06-03 积水医疗株式会社 Latex agglutination inhibition immunoassay

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5992353A (en) * 1982-11-19 1984-05-28 Shinotesuto Kenkyusho:Kk Measuring method of flocculating reaction using insoluble carrier particle
JPH0331227B2 (en) * 1982-11-19 1991-05-02 Shinotesuto Kk
KR20000063268A (en) * 2000-06-13 2000-11-06 김형순 Model Colloid for Diagnostic Usage, and Method for Producing Thereof
CN104685359A (en) * 2012-07-31 2015-06-03 积水医疗株式会社 Latex agglutination inhibition immunoassay
CN104685359B (en) * 2012-07-31 2017-09-05 积水医疗株式会社 Latex agglutination suppresses immunoassays

Also Published As

Publication number Publication date
JPS6352706B2 (en) 1988-10-19

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