JPS5872522A - Preparation of iron-enriched nutriment - Google Patents

Abstract

PURPOSE:The titled nutriment, containing a nicrobial protein in which iron is bonded as a chelate as an iron active constituent in a nutriment consisting of a suitable composition, and capable of improving the iron deficient anemic state. CONSTITUTION:An iron-enriched nutriment containing a microbial protein, e.g. chlorella or yeast, in which iron is bonded as a chelate, as an active constituent for iron supply in a nutriment consisting of a suitable composition, e.g. an enzyme capable of decomposing the cellular membrane. The nutriment has remarkably improved absorbability and very remarkable safety for the living body, and is particularly optimum for improving the anemia in young pigs. The iron content may be adjusted to 1,300mg% or less concentration by incorporating an adequate nutriment therewith. The nutriment is applicable as a convalescent agent for iron deficient anemia.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for producing an iron-enriched nutritional supplement for improving iron-induced anemia, and is particularly useful for improving anemia in piglets.

The amount of hemoglobin (g/dl) at birth of piglets usually shows the same normal value as that of adult pigs, but the amount of hemoglobin decreases rapidly after the beginning of small-feeding, and the value becomes normal by the third month of life. The value reached around 70-70, clearly showing a state of iron-fired anemia, and since this state did not recover easily, i
It is said that this has a negative effect on the growth of piglets. Therefore, conventionally, it has been customary to perform intramuscular injection of iron dextran (approximately 100 mg of iron) in order to bring the hemoglobin level to a normal value.

However, piglets have a very strong ability to absorb iron in their intestinal tracts from the time they are born, and even if they do not rely on artificial injections as mentioned above, if they are given an appropriate iron supplement orally, they can absorb iron as nutrients. It is well absorbed and has the ability to maintain a good iron balance in the body. Furthermore, oral administration of iron preparations is physiologically more natural than relying on the artificial injections described above, and therefore, there is a strong demand for the development of appropriate oral iron preparations.

Oral iron preparations can also be made from inorganic or organic iron salts, but these do not have a very good iron absorption rate, are not physiologically favorable, and may have harmful effects in some cases. also appears.

The present invention has confirmed that when iron is used in a chelate bond with a protein method, the absorption rate is significantly improved and the safety for the living body is extremely remarkable.Based on this, the present invention is aimed at improving anemia in piglets. This makes it possible to produce optimal iron-fortified nutrients.

In other words, the method of the present invention enhances the iron content in a nutritional supplement by containing microbial proteins such as chlorella and yeast that have chelated iron in the nutritional supplement as an active ingredient for iron supplementation. The present invention relates to a method for producing an iron-enriched nutrient, which is characterized in that a cell membrane-degrading enzyme is added to the nutrient used as necessary.

A method for chelating iron ions to microbial proteins such as chlorella and yeast that are used as feed will be specifically explained below using chlorella algae as an example. Chelation of iron ions to yeast is carried out in the same manner as above.Dissolve 20g of iron sulfate (Fe80m, )H,O) in 81g of water and add approximately 1kg of dried Chlorella algae to this, stirring while stirring. After leaving it at room temperature for a while, the supernatant was removed and the residue (iron chelate chlorella) was washed by adding water to remove unreacted iron sulfate dissolved in the water, and this operation was repeated. After separating the residue by drying, the remaining residue is subjected to a drying treatment to obtain iron-chelated chlorella powder. (Yield about 80 cm).

The iron content in this iron chelate chlorella powder varies slightly depending on the chlorella raw material used, but it is approximately 1% in 100g of this product.
The amount of iron chelated with about 1g of 3001F is l OO
It was confirmed by atomic absorption spectrometry that the amount was 0 mg or more. However, when iron is similarly chelated using yeast,
It was confirmed by atomic absorption spectrometry that the amount of iron chelated by yeast was about 500 to 600q, and it was confirmed that the amount of iron chelated by chlorella was larger.

However, chlorella is known to have a relatively high iron and carbon content in foods (loog iron content is 100 to 300).
mg), it is confirmed that the above method strengthens the carbon content of iron by 5 to 10 times. In addition, regarding the administration effect of the above-mentioned iron chelate chlorella powder on iron-induced anemia,
A test using white rats revealed that the dose of iron chelate chlorella powder can be made as low as the dose of chlorella itself, and that the in vivo absorption rate of iron chelate chlorella is extremely high. confirmed.

According to the present invention, the iron content can be adjusted to a concentration of 1300 mg% or less by blending other nutrients as appropriate, and can be widely applied as a recovery agent for iron-induced anemia. A specific example of the composition of the invention method will be described.

Example 1 Iron chelate chlorella powder was produced by the method described above using chlorella algae, and 700 g of this powder was taken, and 150 g each of yeast and wheat bud powder (roasted) were added thereto as other nutrients. and mixed to obtain IKg of iron-enriched nutritional supplement. As a result of atomic absorption spectrometry, the carbon content of iron was 8'78 mg per 100 g.

Example 2 Iron chelate yeast powder was produced using yeast cells in the above-described method. 700 g of this powder was taken, and 150 g each of chlorella and wheat seven sprout powder were added thereto as other nutritional supplements and mixed. Iron-enriched nutritional supplement IKg was obtained. Award: As a result of atomic absorption analysis, the carbon content of iron is 100 gcp45
It was 3 mg.

Example 3 An iron-enriched nutrient was obtained by adding and mixing 3 g of chitalase as a cell membrane degrading enzyme to I kg of the iron-enriched nutrient prepared in Example 1.The carbon content of iron was the same as in Example 1. .

Example 4 An iron-enriched nutrient was obtained by adding and mixing 3 g of chytalase as a cell membrane degrading enzyme to 1" of the iron-enriched nutrient according to Example 2. The carbon content of iron was the same as in Example 2. be.

However, in the nutritional supplements used in Examples 1 and 2, it is significant to add substances that have an animal growth promoting effect, such as chlorella extract, and in the present invention. Nutritional supplements containing such substances may also be used.

Next, a detailed description will be given of the results of a test on the effect of improving anemia in piglets using the iron-enriched nutrient prepared in Example 3 of the present invention.

Eight piglets were divided into a test group (intake of iron-enriched nutrients) and a control group using eight piglets immediately after separation, and the results of rearing for 20 days after birth were observed and observed.

In the test group, each animal received 1 g of the iron-enriched nutritional supplement according to the present invention (according to Example 3) per day and was allowed to breastfeed, while in the control group, animals were allowed to breastfeed only, and both animals were weaned on the 20th day.

The state of recovery from anemia during the test period was measured by hemoglobin level (cyan, methemoglobin method) and hematocrit value (capillary -
Observations were made using the high-speed centrifugation method), reticulocyte value (Pletsker method), and serum iron (Matsubara Minho).

The above measurement results show that the amount of hemoglobin (g/d/
), hematocrit/) value (%), and reticulocyte value (ch) are as shown in Figures 1 to 3, and serum iron was 106 μg/dl in the test group and 233 μg/dl in the control group.
It was μg/61.

As is clear from Figures 1 and 2, the amount of hemoglobin and hematocrit value, which are indicators of iron-induced anemia, are as follows:
Based on the administration of iron-fortified nutritional supplement according to the method of the present invention for 20 days,
It is confirmed that the condition has clearly recovered to above normal values. In addition, looking at the hematopoietic status of the bone marrow, the reticulocyte value (those with high values have a high number of immature red blood cells and a low number of normal red blood cells) was lower than the control group, indicating that red blood cell hematopoiesis. It has also been shown to function well. Furthermore, as mentioned above, the serum iron concentration was clearly much higher in the test group.

In summary, it was determined that the anemic state was completely recovered by administering the iron-fortified nutritional supplement according to the method of the present invention for 20 days. In addition, it was confirmed at a glance that the piglets in the test group had better coat and skin color and luster than piglets in the control group, and were in a healthier state with superior vitality. It was found from Banko that the effect of the iron-fortified nutritional supplement according to the method of the present invention on anemia recovery is extremely remarkable.

The iron-enriched nutritional supplement produced by the method of the present invention described in detail above can be orally taken into the body throughout life, has an extremely good absorption rate, and is extremely effective in improving anemia in piglets. Therefore, the method of the present invention is truly optimal as a method for producing iron-fortified nutrients for animals such as piglets.

[Brief explanation of the drawing]

The figures show the test results for confirming the anemia improvement effect on piglets of the iron-enriched nutritional supplement produced by the method of the present invention. Figure 3 shows the test results for reticulocyte values. Figure 1 Hemoglobin amount (g/dl) Day 0 10 Day Canada Figure 2 Hematocrit value (%) Figure 3 Reticulocyte value (CH) Day 0 10 Day 20

Claims (4)

[Claims] (1) Manufacture of an iron-enriched nutritional supplement, which is characterized by containing a microbial protein chelating iron as an effective iron-replenishing ingredient in a nutritional supplement composed of an appropriate formulation, thereby enhancing the iron content in the nutritional supplement. Law. (2) A method for producing an iron-enriched nutritional supplement according to claim 1, which uses chlorella as the microbial protein. (3) A method for producing an iron-enriched nutritional supplement according to claim 1, which uses yeast as the microbial protein. (4) A method for producing an iron-enriched nutrient according to claims 1 to 3, which uses a nutrient to which a cell membrane degrading enzyme is added.