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Sagami Chemical Research Institute
Tosoh Corp
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Sagami Chemical Research Institute
Toyo Soda Manufacturing Co Ltd
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Application filed by Sagami Chemical Research Institute, Toyo Soda Manufacturing Co LtdfiledCriticalSagami Chemical Research Institute
Priority to JP14508380ApriorityCriticalpatent/JPS5768785A/en
Publication of JPS5768785ApublicationCriticalpatent/JPS5768785A/en
Publication of JPS638748B2publicationCriticalpatent/JPS638748B2/ja
PURPOSE: To In cutting a peptide bond or carrying out a formation reaction using a metal protease, to enhance the activity of metal protease, by using methylamine or methylenediamin.
CONSTITUTION: For example, in forming amino acids shown by the formulasIand II by the formation of a peptide bond between the amino acid shown by the formula I(Z is benzyloxycarbonyl; Asp is aspartic acid residue) and the amino acid shown by the formula II(X is phenylalanine, tryprophan, tyrosine, leucine, isoleucine residue; R is lower alkyl or amino) or by the cutting of peptide bond shown by the formula III(Z, Asp, X, and R are as shown before) through an enzymatic reaction using a metal protease in an aqueous medium, about 0.5W2.5 mole methylamine or methylenediamine per l aqueous medium is added to the reaction system.
COPYRIGHT: (C)1982,JPO&Japio
JP14508380A1980-10-181980-10-18Activity enhancement of protease
GrantedJPS5768785A
(en)
Amino acid and peptide esters of leuco-indo anilines, process for their preparation and agents containing these compounds for the detection of proteolytic enzymes