JPS5779898A - Preparation of human-type insulin - Google Patents

Preparation of human-type insulin

Info

Publication number
JPS5779898A
JPS5779898A JP55154620A JP15462080A JPS5779898A JP S5779898 A JPS5779898 A JP S5779898A JP 55154620 A JP55154620 A JP 55154620A JP 15462080 A JP15462080 A JP 15462080A JP S5779898 A JPS5779898 A JP S5779898A
Authority
JP
Japan
Prior art keywords
insulin
desdipeptide
swine
enzyme
type insulin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP55154620A
Other languages
Japanese (ja)
Other versions
JPH0320237B2 (en
Inventor
Shigenori Tanaka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seikagaku Corp
Original Assignee
Seikagaku Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seikagaku Corp filed Critical Seikagaku Corp
Priority to JP55154620A priority Critical patent/JPS5779898A/en
Publication of JPS5779898A publication Critical patent/JPS5779898A/en
Publication of JPH0320237B2 publication Critical patent/JPH0320237B2/ja
Granted legal-status Critical Current

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PURPOSE: To prepare the titled substance, easily, in high efficiency, by breaking the peptide bonds of swine-type insulin with an enzyme acting specifically to the bond, and forming peptide bonds using the same enzyme.
CONSTITUTION: An enzyme acting specifically to the Pro-Lys bond in the B- chain of swine-type insulin, e.g. peptidase produced by the bacteria belonging to Flavobacterium genus or peptidase separated from the kidney of a lamb, is added to an aqueous medium. Swine-type insulin is hydrolyzed in the aqueous medium to obtain desdipeptide-B29,B30-insulin. The amino group of the desdipeptide-B29,B30-insulin is protected to obtain X2-desdipeptide-B29,B30-insulin, which is condensed with N -X-Lys-Thr in the presence of the above enzyme. The reaction is carried out by using the latter reactant in excess to the former one, and rapidly removing the reaction product from the reaction system.
COPYRIGHT: (C)1982,JPO&Japio
JP55154620A 1980-11-05 1980-11-05 Preparation of human-type insulin Granted JPS5779898A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP55154620A JPS5779898A (en) 1980-11-05 1980-11-05 Preparation of human-type insulin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP55154620A JPS5779898A (en) 1980-11-05 1980-11-05 Preparation of human-type insulin

Publications (2)

Publication Number Publication Date
JPS5779898A true JPS5779898A (en) 1982-05-19
JPH0320237B2 JPH0320237B2 (en) 1991-03-18

Family

ID=15588154

Family Applications (1)

Application Number Title Priority Date Filing Date
JP55154620A Granted JPS5779898A (en) 1980-11-05 1980-11-05 Preparation of human-type insulin

Country Status (1)

Country Link
JP (1) JPS5779898A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0087238A1 (en) * 1982-02-08 1983-08-31 Biogen N.V. Am improved method for preparing human insulin from non-human insulin
CN110305819A (en) * 2019-08-16 2019-10-08 南京工业大学 One plant of feather efficient degrading bacterial strain and its application

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0087238A1 (en) * 1982-02-08 1983-08-31 Biogen N.V. Am improved method for preparing human insulin from non-human insulin
CN110305819A (en) * 2019-08-16 2019-10-08 南京工业大学 One plant of feather efficient degrading bacterial strain and its application

Also Published As

Publication number Publication date
JPH0320237B2 (en) 1991-03-18

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