JPS568684A - Purification of acyl-coenzyme a oxidase - Google Patents
Purification of acyl-coenzyme a oxidaseInfo
- Publication number
- JPS568684A JPS568684A JP8132479A JP8132479A JPS568684A JP S568684 A JPS568684 A JP S568684A JP 8132479 A JP8132479 A JP 8132479A JP 8132479 A JP8132479 A JP 8132479A JP S568684 A JPS568684 A JP S568684A
- Authority
- JP
- Japan
- Prior art keywords
- oxidase
- coenzyme
- acyl
- adsorbed
- pref
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE: To obtain high purity acyl-coenzyme A oxidase, by contacting an acyl- coenzyme A oxidase-containing liquid with a compound bonded to a water-soluble carrier, and eluting the adsorbed oxidase.
CONSTITUTION: A solution containing acyl-coenzyme A oxidase is brought into contact with a water-soluble carrier bonded with a group of formula CH3-(CH2)n- (n is 1W3) or NH2-(CH2)m-(m is 2W8), and the adsorbed acyl-coenzyme A oxidase is eluted to obtain the purified objective product. The acyl-coenzyme A oxidase- containing liquid can be prepared by culturing various microorganisms in an enriched medium. The medium pref. contains 0.5% of oleic acid, 0.5% of palmitic acid, 0.3% of yeast extract, 0.5% of (NH4)H2PO4, 0.25% of KH2PO4, and 0.1% of MgSO4.7H2O, and the culturing is pref. carried out aerobically, at 20W40°C for 1W5 days.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP8132479A JPS6013664B2 (en) | 1979-06-29 | 1979-06-29 | Method for purifying acyl coenzyme A oxidase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP8132479A JPS6013664B2 (en) | 1979-06-29 | 1979-06-29 | Method for purifying acyl coenzyme A oxidase |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS568684A true JPS568684A (en) | 1981-01-29 |
JPS6013664B2 JPS6013664B2 (en) | 1985-04-09 |
Family
ID=13743207
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP8132479A Expired JPS6013664B2 (en) | 1979-06-29 | 1979-06-29 | Method for purifying acyl coenzyme A oxidase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6013664B2 (en) |
-
1979
- 1979-06-29 JP JP8132479A patent/JPS6013664B2/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
JPS6013664B2 (en) | 1985-04-09 |
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