JPS5651983A - Preparation of fixed protease - Google Patents

Preparation of fixed protease

Info

Publication number
JPS5651983A
JPS5651983A JP12928679A JP12928679A JPS5651983A JP S5651983 A JPS5651983 A JP S5651983A JP 12928679 A JP12928679 A JP 12928679A JP 12928679 A JP12928679 A JP 12928679A JP S5651983 A JPS5651983 A JP S5651983A
Authority
JP
Japan
Prior art keywords
protease
fibroin
aqueous solution
stability
fixed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP12928679A
Other languages
Japanese (ja)
Other versions
JPS6013673B2 (en
Inventor
Kenichi Otsubo
Hiroshi Nakayama
Hiroshi Jinno
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kanebo Ltd
Original Assignee
Kanebo Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kanebo Ltd filed Critical Kanebo Ltd
Priority to JP12928679A priority Critical patent/JPS6013673B2/en
Publication of JPS5651983A publication Critical patent/JPS5651983A/en
Publication of JPS6013673B2 publication Critical patent/JPS6013673B2/en
Expired legal-status Critical Current

Links

Abstract

PURPOSE: To prepare fixed protease having improved stability, especially improved heat stability, pH stability, stability with time, etc., by depositing fibroin and protease in an aqueous solution of fibroin through salting out.
CONSTITUTION: An aqueous solution of fibroin with a fibroin concentration 2W 20wt% is blended with an aqueous solution of an alkali protease and/or neutral protease with a protease concentration 0.5W30wt% in pH 3W3.5 at 0W15°C and the blend is adjusted to pH 3W5.5. The blend is added to an aqueous solution of ammonium sulfate and adjusted to pH 5.0W9.0, preferably 5.5W8.0 so that fibroin and protease are deposited through salting out. The precipitate is washed with water and dried at not higher than 60°C to give fixed protease containing 0.1W20wt%, preferably 1W15wt% alkali or neutral protease.
COPYRIGHT: (C)1981,JPO&Japio
JP12928679A 1979-10-05 1979-10-05 Method for producing immobilized protease Expired JPS6013673B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP12928679A JPS6013673B2 (en) 1979-10-05 1979-10-05 Method for producing immobilized protease

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP12928679A JPS6013673B2 (en) 1979-10-05 1979-10-05 Method for producing immobilized protease

Publications (2)

Publication Number Publication Date
JPS5651983A true JPS5651983A (en) 1981-05-09
JPS6013673B2 JPS6013673B2 (en) 1985-04-09

Family

ID=15005816

Family Applications (1)

Application Number Title Priority Date Filing Date
JP12928679A Expired JPS6013673B2 (en) 1979-10-05 1979-10-05 Method for producing immobilized protease

Country Status (1)

Country Link
JP (1) JPS6013673B2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7007493B2 (en) * 2018-10-03 2022-01-24 オリンパス株式会社 Power supply for endoscopes

Also Published As

Publication number Publication date
JPS6013673B2 (en) 1985-04-09

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