JPS5651983A - Preparation of fixed protease - Google Patents
Preparation of fixed proteaseInfo
- Publication number
- JPS5651983A JPS5651983A JP12928679A JP12928679A JPS5651983A JP S5651983 A JPS5651983 A JP S5651983A JP 12928679 A JP12928679 A JP 12928679A JP 12928679 A JP12928679 A JP 12928679A JP S5651983 A JPS5651983 A JP S5651983A
- Authority
- JP
- Japan
- Prior art keywords
- protease
- fibroin
- aqueous solution
- stability
- fixed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
PURPOSE: To prepare fixed protease having improved stability, especially improved heat stability, pH stability, stability with time, etc., by depositing fibroin and protease in an aqueous solution of fibroin through salting out.
CONSTITUTION: An aqueous solution of fibroin with a fibroin concentration 2W 20wt% is blended with an aqueous solution of an alkali protease and/or neutral protease with a protease concentration 0.5W30wt% in pH 3W3.5 at 0W15°C and the blend is adjusted to pH 3W5.5. The blend is added to an aqueous solution of ammonium sulfate and adjusted to pH 5.0W9.0, preferably 5.5W8.0 so that fibroin and protease are deposited through salting out. The precipitate is washed with water and dried at not higher than 60°C to give fixed protease containing 0.1W20wt%, preferably 1W15wt% alkali or neutral protease.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12928679A JPS6013673B2 (en) | 1979-10-05 | 1979-10-05 | Method for producing immobilized protease |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP12928679A JPS6013673B2 (en) | 1979-10-05 | 1979-10-05 | Method for producing immobilized protease |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5651983A true JPS5651983A (en) | 1981-05-09 |
JPS6013673B2 JPS6013673B2 (en) | 1985-04-09 |
Family
ID=15005816
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP12928679A Expired JPS6013673B2 (en) | 1979-10-05 | 1979-10-05 | Method for producing immobilized protease |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6013673B2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP7007493B2 (en) * | 2018-10-03 | 2022-01-24 | オリンパス株式会社 | Power supply for endoscopes |
-
1979
- 1979-10-05 JP JP12928679A patent/JPS6013673B2/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
JPS6013673B2 (en) | 1985-04-09 |
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