JPS5615687A - Immobilized protease and its preparation - Google Patents

Immobilized protease and its preparation

Info

Publication number
JPS5615687A
JPS5615687A JP8970979A JP8970979A JPS5615687A JP S5615687 A JPS5615687 A JP S5615687A JP 8970979 A JP8970979 A JP 8970979A JP 8970979 A JP8970979 A JP 8970979A JP S5615687 A JPS5615687 A JP S5615687A
Authority
JP
Japan
Prior art keywords
protease
fibroin
aqueous solution
solution
alkali
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP8970979A
Other languages
Japanese (ja)
Other versions
JPS6013672B2 (en
Inventor
Hiroshi Nakayama
Yukio Horikawa
Tetsuro Oota
Kenichi Otsubo
Hiroshi Jinno
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kanebo Ltd
Original Assignee
Kanebo Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kanebo Ltd filed Critical Kanebo Ltd
Priority to JP8970979A priority Critical patent/JPS6013672B2/en
Publication of JPS5615687A publication Critical patent/JPS5615687A/en
Publication of JPS6013672B2 publication Critical patent/JPS6013672B2/en
Expired legal-status Critical Current

Links

Abstract

PURPOSE: To produce an immobilized protease having improved thermal stability and pH stability industrially and at a low cost, by incorporating an alkali protease and/or neutral protease in a specific wt% into fibroin.
CONSTITUTION: An aqueous solution of fibroin is incorporated with an aqueous solution of an alkali protease and/or neutral protease adjusted to pH5W5.5. The fibroin and the protease are salted out and precipitated by the use of an inorganic and/or organic salt, and the resulted precipitate is washed with water and dried to prepare an immobilized protease. The aqueous solution of fibroin is obtaned by dissolving sericin scoured and removed from silk, e.g. raw silk or cocoon, in an aqueous solution of an alkali metal salt, or further dialyzing and desalting the solution, and the concentration of the equeous solution is preferably 2W20wt%.
COPYRIGHT: (C)1981,JPO&Japio
JP8970979A 1979-07-13 1979-07-13 Immobilized protease and its production method Expired JPS6013672B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP8970979A JPS6013672B2 (en) 1979-07-13 1979-07-13 Immobilized protease and its production method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP8970979A JPS6013672B2 (en) 1979-07-13 1979-07-13 Immobilized protease and its production method

Publications (2)

Publication Number Publication Date
JPS5615687A true JPS5615687A (en) 1981-02-14
JPS6013672B2 JPS6013672B2 (en) 1985-04-09

Family

ID=13978291

Family Applications (1)

Application Number Title Priority Date Filing Date
JP8970979A Expired JPS6013672B2 (en) 1979-07-13 1979-07-13 Immobilized protease and its production method

Country Status (1)

Country Link
JP (1) JPS6013672B2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62188177U (en) * 1986-05-22 1987-11-30
JP3024212U (en) * 1995-10-30 1996-05-17 有限会社ゴーイング東京 Filter element for engine air cleaner

Also Published As

Publication number Publication date
JPS6013672B2 (en) 1985-04-09

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