JPS5639783A - Fixed protease and its preparation - Google Patents

Fixed protease and its preparation

Info

Publication number
JPS5639783A
JPS5639783A JP11319679A JP11319679A JPS5639783A JP S5639783 A JPS5639783 A JP S5639783A JP 11319679 A JP11319679 A JP 11319679A JP 11319679 A JP11319679 A JP 11319679A JP S5639783 A JPS5639783 A JP S5639783A
Authority
JP
Japan
Prior art keywords
protease
fibroin
stability
fixed
acidic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP11319679A
Other languages
Japanese (ja)
Other versions
JPS6158160B2 (en
Inventor
Hiroshi Nakayama
Kenichi Otsubo
Hiroshi Jinno
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kanebo Ltd
Original Assignee
Kanebo Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kanebo Ltd filed Critical Kanebo Ltd
Priority to JP11319679A priority Critical patent/JPS5639783A/en
Publication of JPS5639783A publication Critical patent/JPS5639783A/en
Publication of JPS6158160B2 publication Critical patent/JPS6158160B2/ja
Granted legal-status Critical Current

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  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PURPOSE: To prepare a fixed protease having improved stability, especially thermal stability, pH stability, and stability with time, by adding an acidic protease to fibroin.
CONSTITUTION: An aqueous solution of fibroin having a fibroin concentration 2W20wt% is blended with an aqueous solution of acidic protease having a pH 8W11 and a protease concentration 0.5W30wt% at a solution temperature of 0W15°C to give a mixed solution, which is adjusted to pH 8W11, preferably 8.5W10.5, and fibroin and protease are precipitaed through salting out by using an inorganic salt and/or organic salt, e.g., ammonium sulfate, sodium citrate, etc. to prepare precipitate. The precipitate is wahsed with water with pH 8W11 and dried at not higher than 60°C at normal pressure or under reduced pressure to give a fixed protease containing 0.1W20wt%, preferably 1W15wt% acidic protease in a powdered fibroin.
COPYRIGHT: (C)1981,JPO&Japio
JP11319679A 1979-09-03 1979-09-03 Fixed protease and its preparation Granted JPS5639783A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP11319679A JPS5639783A (en) 1979-09-03 1979-09-03 Fixed protease and its preparation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP11319679A JPS5639783A (en) 1979-09-03 1979-09-03 Fixed protease and its preparation

Publications (2)

Publication Number Publication Date
JPS5639783A true JPS5639783A (en) 1981-04-15
JPS6158160B2 JPS6158160B2 (en) 1986-12-10

Family

ID=14605977

Family Applications (1)

Application Number Title Priority Date Filing Date
JP11319679A Granted JPS5639783A (en) 1979-09-03 1979-09-03 Fixed protease and its preparation

Country Status (1)

Country Link
JP (1) JPS5639783A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0297559A (en) * 1988-10-03 1990-04-10 Toshiba Silicone Co Ltd Heat-conductive silicone composition
JPH0488060A (en) * 1990-08-01 1992-03-19 Shin Etsu Chem Co Ltd Oganopolysiloxane composition and cured gel thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0297559A (en) * 1988-10-03 1990-04-10 Toshiba Silicone Co Ltd Heat-conductive silicone composition
JPH0536456B2 (en) * 1988-10-03 1993-05-31 Toshiba Silicone
JPH0488060A (en) * 1990-08-01 1992-03-19 Shin Etsu Chem Co Ltd Oganopolysiloxane composition and cured gel thereof

Also Published As

Publication number Publication date
JPS6158160B2 (en) 1986-12-10

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