JPS5621600A - Determination of lipid peroxide - Google Patents

Determination of lipid peroxide

Info

Publication number
JPS5621600A
JPS5621600A JP9616579A JP9616579A JPS5621600A JP S5621600 A JPS5621600 A JP S5621600A JP 9616579 A JP9616579 A JP 9616579A JP 9616579 A JP9616579 A JP 9616579A JP S5621600 A JPS5621600 A JP S5621600A
Authority
JP
Japan
Prior art keywords
lipid peroxide
reacting
glutathione
determination
gsh
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP9616579A
Other languages
Japanese (ja)
Other versions
JPS5729158B2 (en
Inventor
Michiya Kimura
Toshiaki Miura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HORIUCHI ITAROU SHIYOUTEN KK
HORIUCHI ITAROU SHOTEN KK
Yamasa Shoyu KK
Original Assignee
HORIUCHI ITAROU SHIYOUTEN KK
HORIUCHI ITAROU SHOTEN KK
Yamasa Shoyu KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HORIUCHI ITAROU SHIYOUTEN KK, HORIUCHI ITAROU SHOTEN KK, Yamasa Shoyu KK filed Critical HORIUCHI ITAROU SHIYOUTEN KK
Priority to JP9616579A priority Critical patent/JPS5621600A/en
Publication of JPS5621600A publication Critical patent/JPS5621600A/en
Publication of JPS5729158B2 publication Critical patent/JPS5729158B2/ja
Granted legal-status Critical Current

Links

Landscapes

  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To provide a simple and highly sensitive means for the determination of lipid peroxide, by reacting a specimen containing lipid peroxide with glutathione peroxidase in the presence of reduced glutathione, and converting the produced oxidized glutathione to a fluorescent material.
CONSTITUTION: A highly sensitive micro-determination of th degree of peroxidation of a lipid peroxide in the living body is carried out by (1) reacting the lipid peroxide with glutathione peroxidase in the presence of reduced glutathione (GSH) to convert GSH to oxidized glutathione (GSSG), (2) reacting and eliminating the residual unreacted GSH with an SH reagent (e.g. N-ethylmaleimide), (3) making the reaction mixture to alkaline state, (4) reacting GSSG with o-phthalaldehyde, (5) exciting the produced fluorescent material with light having a wavelength of 343nm and measuring the intensity of the emitting light at a wavelength of 420nm, and (6) determining the concentration of the lipid peroxide in the specimen from a calibration curruve prepared by using specimens having known concentrations.
COPYRIGHT: (C)1981,JPO&Japio
JP9616579A 1979-07-30 1979-07-30 Determination of lipid peroxide Granted JPS5621600A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP9616579A JPS5621600A (en) 1979-07-30 1979-07-30 Determination of lipid peroxide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP9616579A JPS5621600A (en) 1979-07-30 1979-07-30 Determination of lipid peroxide

Publications (2)

Publication Number Publication Date
JPS5621600A true JPS5621600A (en) 1981-02-28
JPS5729158B2 JPS5729158B2 (en) 1982-06-21

Family

ID=14157716

Family Applications (1)

Application Number Title Priority Date Filing Date
JP9616579A Granted JPS5621600A (en) 1979-07-30 1979-07-30 Determination of lipid peroxide

Country Status (1)

Country Link
JP (1) JPS5621600A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56117798A (en) * 1980-02-21 1981-09-16 Toyo Jozo Co Ltd Kit for measuring lipoid peroxide

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5718999A (en) * 1980-07-09 1982-01-30 Fumio Kuzutani Enzymatic measurement of hydro-type peroxidized lipid
JPS60254700A (en) * 1984-05-31 1985-12-16 富士通株式会社 Cooling liquid dipping circuit board

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56117798A (en) * 1980-02-21 1981-09-16 Toyo Jozo Co Ltd Kit for measuring lipoid peroxide
US4367285A (en) * 1980-02-21 1983-01-04 Toyo Jozo Company, Ltd. Assaying lipid peroxide in lipid compositions
JPS6236680B2 (en) * 1980-02-21 1987-08-07 Toyo Jozo Kk

Also Published As

Publication number Publication date
JPS5729158B2 (en) 1982-06-21

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