JPS5718999A - Enzymatic measurement of hydro-type peroxidized lipid - Google Patents
Enzymatic measurement of hydro-type peroxidized lipidInfo
- Publication number
- JPS5718999A JPS5718999A JP9260280A JP9260280A JPS5718999A JP S5718999 A JPS5718999 A JP S5718999A JP 9260280 A JP9260280 A JP 9260280A JP 9260280 A JP9260280 A JP 9260280A JP S5718999 A JPS5718999 A JP S5718999A
- Authority
- JP
- Japan
- Prior art keywords
- glutathion
- lipid
- hydro
- type
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
PURPOSE: By the action of glutathion peroxidase, oxidized glutathion is formed from glutathion and titled lipid, futher NADP is formed with a reductase in the presence of NADPH to measure the reduction in NADPH whereby the titled substance is determined.
CONSTITUTION: A glutathion reductace solution, a glutathion solution, and an NADPH solution are placed together with a buffer solution in a test tube and further, a buffer solution of glutathion peroxidase and a hydro-type peroxidized lipid or a lipid containing the same as added to the test tube. The mixture is combined with a surfactant such as sodium dodecylsulfate or Triton-X and/or an alcohol to effeft the enzymatic reactions and the light absorbance is measured before and after the reaction respectively to know the difference and calculate the amount of the hydro-type peroxidized lipid.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9260280A JPS5718999A (en) | 1980-07-09 | 1980-07-09 | Enzymatic measurement of hydro-type peroxidized lipid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9260280A JPS5718999A (en) | 1980-07-09 | 1980-07-09 | Enzymatic measurement of hydro-type peroxidized lipid |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5718999A true JPS5718999A (en) | 1982-01-30 |
Family
ID=14058995
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP9260280A Pending JPS5718999A (en) | 1980-07-09 | 1980-07-09 | Enzymatic measurement of hydro-type peroxidized lipid |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5718999A (en) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5729158B2 (en) * | 1979-07-30 | 1982-06-21 |
-
1980
- 1980-07-09 JP JP9260280A patent/JPS5718999A/en active Pending
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5729158B2 (en) * | 1979-07-30 | 1982-06-21 |
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