JPS5718999A - Enzymatic measurement of hydro-type peroxidized lipid - Google Patents

Enzymatic measurement of hydro-type peroxidized lipid

Info

Publication number
JPS5718999A
JPS5718999A JP9260280A JP9260280A JPS5718999A JP S5718999 A JPS5718999 A JP S5718999A JP 9260280 A JP9260280 A JP 9260280A JP 9260280 A JP9260280 A JP 9260280A JP S5718999 A JPS5718999 A JP S5718999A
Authority
JP
Japan
Prior art keywords
glutathion
lipid
hydro
type
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP9260280A
Other languages
Japanese (ja)
Inventor
Fumio Kuzutani
Kenichi Hirano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP9260280A priority Critical patent/JPS5718999A/en
Publication of JPS5718999A publication Critical patent/JPS5718999A/en
Pending legal-status Critical Current

Links

Abstract

PURPOSE: By the action of glutathion peroxidase, oxidized glutathion is formed from glutathion and titled lipid, futher NADP is formed with a reductase in the presence of NADPH to measure the reduction in NADPH whereby the titled substance is determined.
CONSTITUTION: A glutathion reductace solution, a glutathion solution, and an NADPH solution are placed together with a buffer solution in a test tube and further, a buffer solution of glutathion peroxidase and a hydro-type peroxidized lipid or a lipid containing the same as added to the test tube. The mixture is combined with a surfactant such as sodium dodecylsulfate or Triton-X and/or an alcohol to effeft the enzymatic reactions and the light absorbance is measured before and after the reaction respectively to know the difference and calculate the amount of the hydro-type peroxidized lipid.
COPYRIGHT: (C)1982,JPO&Japio
JP9260280A 1980-07-09 1980-07-09 Enzymatic measurement of hydro-type peroxidized lipid Pending JPS5718999A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP9260280A JPS5718999A (en) 1980-07-09 1980-07-09 Enzymatic measurement of hydro-type peroxidized lipid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP9260280A JPS5718999A (en) 1980-07-09 1980-07-09 Enzymatic measurement of hydro-type peroxidized lipid

Publications (1)

Publication Number Publication Date
JPS5718999A true JPS5718999A (en) 1982-01-30

Family

ID=14058995

Family Applications (1)

Application Number Title Priority Date Filing Date
JP9260280A Pending JPS5718999A (en) 1980-07-09 1980-07-09 Enzymatic measurement of hydro-type peroxidized lipid

Country Status (1)

Country Link
JP (1) JPS5718999A (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5729158B2 (en) * 1979-07-30 1982-06-21

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5729158B2 (en) * 1979-07-30 1982-06-21

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