JPS56158092A - Preparation of heat-resistant glucokinase - Google Patents
Preparation of heat-resistant glucokinaseInfo
- Publication number
- JPS56158092A JPS56158092A JP6318080A JP6318080A JPS56158092A JP S56158092 A JPS56158092 A JP S56158092A JP 6318080 A JP6318080 A JP 6318080A JP 6318080 A JP6318080 A JP 6318080A JP S56158092 A JPS56158092 A JP S56158092A
- Authority
- JP
- Japan
- Prior art keywords
- culture
- molds
- centrifuging
- strain
- glucokinase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To prepare the titled substance easily by industrially large scale production, by cultivating a thermophilic bacterium belonging to "Bacillus stearothermophilus".
CONSTITUTION: UK788 strain (FERM P 5141) belonging to "Bacillus stearothermophilus" is cultivated especially by liquid culture in a general medium comprising common untrient sources of bacteria, e.g., carbon source such as glucose, molasses, etc., nitrogen source such as urea, ammonium sulfate, inorganic salts such as K, Na, Cu, etc. In the case of batch cultivation, the culture is preferably carried out until the latter period of logarithmic propagation, and in the case of continuous cultivation, continuous culture of the substance circulation type is adopted, and the rate of dilution is controlled in the vicinity of the maximum rate of the relative propagation of UK788 strain. Molds are collected from the culture by centrifuging, etc. Heat-resistant glucokinase is isolated and purified by a conventional method, e.g., adding an organic solvent, etc. to a supernatant liquid obtained by grinding the molds and centrifuging to attain purification through fractionation, adsorbing the molds on a carrier followed by purification, etc.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6318080A JPS56158092A (en) | 1980-05-12 | 1980-05-12 | Preparation of heat-resistant glucokinase |
| DK496080A DK496080A (en) | 1979-11-22 | 1980-11-20 | MICROORGANISM CULTURE AND ITS APPLICATION FOR MANUFACTURING INTRACELLULAR PRODUCTS |
| CA000365249A CA1156573A (en) | 1980-04-18 | 1980-11-21 | Strain uk 788 and process for producing a useful enzyme |
| EP80107281A EP0029976B1 (en) | 1979-11-22 | 1980-11-21 | A biologically pure culture of strain ifo 14093 and a process for producing an intracellular component herefrom |
| DE8080107281T DE3070039D1 (en) | 1979-11-22 | 1980-11-21 | A biologically pure culture of strain ifo 14093 and a process for producing an intracellular component herefrom |
| US06/209,097 US4331762A (en) | 1980-04-18 | 1980-11-21 | Bacillus stearothermophilus strain UK 788 and process for producing a useful enzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6318080A JPS56158092A (en) | 1980-05-12 | 1980-05-12 | Preparation of heat-resistant glucokinase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS56158092A true JPS56158092A (en) | 1981-12-05 |
| JPS6251590B2 JPS6251590B2 (en) | 1987-10-30 |
Family
ID=13221778
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6318080A Granted JPS56158092A (en) | 1979-11-22 | 1980-05-12 | Preparation of heat-resistant glucokinase |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS56158092A (en) |
-
1980
- 1980-05-12 JP JP6318080A patent/JPS56158092A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6251590B2 (en) | 1987-10-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE314463T1 (en) | MALTOSE-TREHALOSE CONVERTING ENZYME, PRODUCTION AND USE THEREOF | |
| EE03114B1 (en) | Method for the production of cyclosporin A. | |
| JPS56160998A (en) | Production of biotin active substance biotin vitamer | |
| JPS56158092A (en) | Preparation of heat-resistant glucokinase | |
| JPS5688799A (en) | Preparation of l-lysine | |
| JPS5675097A (en) | Thermophilic aspartase and its preparation | |
| JPS52125686A (en) | Process for growing yeast in high yield | |
| ATE22702T1 (en) | PRODUCTION OF GLUCOSE DEHYDROGENASE. | |
| JPS5682099A (en) | Production of glutathion | |
| JPS55114295A (en) | Production of guanosine by fermentation | |
| EP0110422A3 (en) | Process for producing l-aspartic acid | |
| GB1084431A (en) | Improvements in and relating to lipases | |
| JPS6474999A (en) | Production of optically active (r)-(-)-beta-hydroxylactic acid | |
| JPS5675095A (en) | Preparation of heat-resistant acetic acid kinase | |
| US3792160A (en) | Method of treating inflammation and composition therefor | |
| JPS5799198A (en) | Preparation of l-phenylalanine | |
| JPS5234984A (en) | Production of adenine derivatives by fermentation process | |
| JPS5648891A (en) | Preparation of polysaccharide | |
| JPS57138387A (en) | Preparation of branching enzyme | |
| JPS6463391A (en) | Production of free protein a | |
| JPS57129695A (en) | Preparation of coenzyme q10 by fermentation process | |
| JPS5791191A (en) | Production of cell bodies of high content of pyruvate kinase | |
| JPS5432686A (en) | Flocculation of microorganisms | |
| JPS57146574A (en) | Preparation of nucleotide pyrophosphatase | |
| JPS6439996A (en) | Production of l-threonine by fermentation |